TRPV4 channels in the human urogenital tract play a role in cell junction formation and epithelial barrier.
until further notice
SourceActa Physiologica, 218, 1, (2016), pp. 38-48
Article / Letter to editor
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SubjectRadboudumc 10: Reconstructive and regenerative medicine RIMLS: Radboud Institute for Molecular Life Sciences; Radboudumc 11: Renal disorders RIMLS: Radboud Institute for Molecular Life Sciences; Radboudumc 15: Urological cancers RIMLS: Radboud Institute for Molecular Life Sciences
AIM: The molecular interactions between Transient Receptor Potential Vanilloid subtype 4 channels (TRPV4) and cell junction formation were investigated in the human and mouse urogenital tract. MATERIALS AND METHODS: A qualitative study was performed to investigate TRPV4 channels, adherence junctions (AJ's) and tight junctions (TJ's) in kidney, ureter and bladder tissues from humans and wild type and transgenic TRPV4 knockout (-/-) mice with immunohistochemistry, western blotting, immunoprecipitation and reverse-trasnscription-PCR. Cell junction formation in the wild type and TRPV4 knockout (-/-) mouse was evaluated with immunohistochemistry and Transmission Electron Microscope (TEM) techniques. RESULTS: TRPV4 channels are predominantly located in membranes of epithelial cells of the bladder, ureter and the collecting ducts of the kidney. There is a molecular interaction between the TRPV4 channel and the AJ. TEM evaluation showed that AJ formation is disrupted in the TRPV4 -/- mouse resulting in deficient intercellular connections and integrity of the epithelium. CONCLUSIONS: TRPV4 is believed to be a mechanoreceptor in the bladder. This study demonstrates that TRPV4 is also involved in intercellular connectivity and structural integrity of the epithelium. This article is protected by copyright. All rights reserved.
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