Publication year
2016Source
Radiation Research, 185, 5, (2016), pp. 496-504ISSN
Publication type
Article / Letter to editor

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Organization
Radiation Oncology
Journal title
Radiation Research
Volume
vol. 185
Issue
iss. 5
Page start
p. 496
Page end
p. 504
Subject
Radboudumc 9: Rare cancers RIHS: Radboud Institute for Health SciencesAbstract
Quantitative data is presented that shows significant changes in cellular metabolism in a head and neck cancer cell line 30 min after irradiation. A head and neck cancer cell line (UM-SCC-22B) and a comparable normal cell line, normal oral keratinocyte (NOK) were each separately exposed to 10 Gy and treated with a control drug for disrupting metabolism (potassium cyanide; KCN). The metabolic changes were measured live by fluorescence lifetime imaging of the intrinsically fluorescent intermediate metabolite nicotinamide adenosine dinucleotide (NADH) fluorescence; this method is sensitive to the ratio of bound to free NADH. The results indicated a prompt shift in metabolic signature in the cancer cell line, but not in the normal cell line. Control KCN treatment demonstrated expected metabolic fluxes due to mitochondrial disruption. The detected radiation shift in the cancer cells was blunted in the presence of both a radical scavenger and a HIF-1alpha inhibitor. The HIF-1alpha abundance as detected by immunohistochemical staining also increased substantially for these cancer cells, but not for the normal cells. This type of live-cell metabolic monitoring could be helpful for future real-time studies and in designing adaptive radiotherapy approaches.
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- Academic publications [227613]
- Faculty of Medical Sciences [86193]
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