New Biological Insights from Better Structure Models
Publication year
2016Source
Journal of Molecular Biology, 428, 6, (2016), pp. 1375-93ISSN
Publication type
Article / Letter to editor

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Organization
CMBI
Journal title
Journal of Molecular Biology
Volume
vol. 428
Issue
iss. 6
Page start
p. 1375
Page end
p. 93
Subject
Radboudumc 19: Nanomedicine RIMLS: Radboud Institute for Molecular Life SciencesAbstract
Structure validation is a key component of all steps in the structure determination process, from structure building, refinement, deposition, and evaluation all the way to post-deposition optimisation of structures in the Protein Data Bank (PDB) by re-refinement and re-building. Today, many aspects of protein structures are understood better than 10years ago, and combined with improved software and more computing power, the automated PDB_REDO procedure can significantly improve about 85% of all X-ray structures ever deposited in the PDB. We review structure validation, structure improvement, and a series of validation resources and facilities that give access to improved PDB files and to reports on the quality of the original and the improved structures. Post-deposition optimisation generally leads to improved protein structures and a series of examples will illustrate how that, in turn, leads to improved or even novel biological insights.
This item appears in the following Collection(s)
- Academic publications [202802]
- Electronic publications [100870]
- Faculty of Medical Sciences [80020]
- Open Access publications [69592]
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