Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

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Title:	Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells
Author(s):	Nagelkerke, A.P.; Span, P.N
Publication year:	2016
In:	Tumor Microenvironment: Study Protocols, pp. 1-10
Publisher:	Cham : Springer Int Publishing Ag
ISBN:	00652598
Series:	Advances in Experimental Medicine and Biology ; 899
DOI:	http://dx.doi.org/10.1007/978-3-319-26666-4_1
Publication type:	Part of book or chapter of book
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/166262
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Subject:	Molecular Materials
Organization:	Molecular Materials
Book title:	Tumor Microenvironment: Study Protocols
Page start:	p. 1
Page end:	p. 10
Abstract:	Phospho-H2AX or gamma-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against gamma-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.