Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells
Publication year
2016Publisher
Cham : Springer Int Publishing Ag
Series
Advances in Experimental Medicine and Biology ; 899
ISBN
9783319266640
In
Tumor Microenvironment: Study Protocols, pp. 1-10Publication type
Part of book or chapter of book

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Organization
Molecular Materials
Book title
Tumor Microenvironment: Study Protocols
Page start
p. 1
Page end
p. 10
Subject
Advances in Experimental Medicine and Biology; Molecular MaterialsAbstract
Phospho-H2AX or gamma-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against gamma-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.
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- Academic publications [227881]
- Faculty of Science [33782]
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