Author(s):
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Ma, L.; Kutty, G.; Jia, Q.; Imamichi, H.; Huang, L.; Atzori, C.;
Beckers, P.J.A.
; Groner, G.; Beard, C.B.; Kovacs, J.A.
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Subject:
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Malaria: parasite-host interaction Malaria: gastheer-parasiet interactie |
Journal title:
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The Journal of Infectious Diseases
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Abstract:
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Variation in the tandem repeats in the expression site of the human-derived Pneumocystis carinii major surface glycoprotein gene was characterized by denaturing gel electrophoresis. The number of repeats in 147 isolates ranged from 2 to 6, with 2, 3, and 4 repeats being the most common. Sequence analysis identified 3 types of repeat units that differed by 1 nucleotide, which suggests a hierarchy of evolution of human-derived P. carinii. Examination of sequential samples obtained from 6 patients at an interval of 10-90 days showed an identical repeat pattern in each patient. However, in 2 of 4 patients with 2-3 different samples obtained within 4 days, different repeat patterns were observed among the samples. Quantifying the number of repeats by denaturing gel electrophoresis is a simple and rapid-typing method that can be used alone or in combination with other methods to study the epidemiology of P. carinii.
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