Analysis of variation in tandem repeats in the intron of the major surface glycoprotein expression site of the human form of Pneumocystis carinii.

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Title:	Analysis of variation in tandem repeats in the intron of the major surface glycoprotein expression site of the human form of Pneumocystis carinii.
Author(s):	Ma, L.; Kutty, G.; Jia, Q.; Imamichi, H.; Huang, L.; Atzori, C.; Beckers, P.J.A. ; Groner, G.; Beard, C.B.; Kovacs, J.A.
Publication year:	2002
Source:	Journal of Infectious Diseases, vol. 186, iss. 11, (2002), pp. 1647-1654
ISSN:	0022-1899
DOI:	https://doi.org/10.1086/345721
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/160888
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Subject:	Malaria: parasite-host interaction Malaria: gastheer-parasiet interactie
Organization:	Medical Microbiology
Journal title:	Journal of Infectious Diseases
Volume:	vol. 186
Issue:	iss. 11
Page start:	p. 1647
Page end:	p. 1654
Abstract:	Variation in the tandem repeats in the expression site of the human-derived Pneumocystis carinii major surface glycoprotein gene was characterized by denaturing gel electrophoresis. The number of repeats in 147 isolates ranged from 2 to 6, with 2, 3, and 4 repeats being the most common. Sequence analysis identified 3 types of repeat units that differed by 1 nucleotide, which suggests a hierarchy of evolution of human-derived P. carinii. Examination of sequential samples obtained from 6 patients at an interval of 10-90 days showed an identical repeat pattern in each patient. However, in 2 of 4 patients with 2-3 different samples obtained within 4 days, different repeat patterns were observed among the samples. Quantifying the number of repeats by denaturing gel electrophoresis is a simple and rapid-typing method that can be used alone or in combination with other methods to study the epidemiology of P. carinii.