The matrix metalloproteinase inhibitor BB-94 improves the strength of intestinal anastomoses in the rat.
SourceInternational Journal of Colorectal Disease, 17, 5, (2002), pp. 348-54
Article / Letter to editor
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International Journal of Colorectal Disease
SubjectSepsis and non-bacterial generalized inflammation: causes and effects (sepsis and inflammation); Sepsis en niet-bacteriële gegeneraliseerde ontsteking: mogelijke oorzaken en gevolgen (sepsis en ontsteking)
BACKGROUND AND AIMS: The strength of intestinal anastomoses is relatively low in the first days after operation, possibly as a result of localized degradation of the supporting matrix by enzymes from the matrix metalloproteinase (MMP) family. This study examined whether BB-94, a broad spectrum inhibitor of MMP activity, could enhance anastomotic strength. MATERIALS AND METHODS: Male Wistar rats received anastomoses in both ileum and colon. From the day before operation onwards, animals were treated daily with BB-94 intraperitoneally at a dose of 30 mg/kg or with saline only. Rats were killed 1, 3, or 7 days after operation, and anastomotic bursting pressure and breaking strength were measured. On day 3 anastomotic hydroxyproline levels were measured, and MMP (gelatinase) activity was analyzed by gelatin zymography. RESULTS: BB-94 strongly enhanced wound strength, but only on day 3, when it was at its lowest. Daily administration increased median colonic and ileal breaking strength by 27% and 108%, respectively; colonic and ileal bursting pressure were increased by 54% and 58%, respectively. MMP activities were significantly lowered in anastomotic extracts from the rats treated with BB-94.CONCLUSION: Administration of BB-94 enhances anastomotic strength. Specific inhibition of MMP activity should be investigated further as a means to preserve anastomotic integrity.
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- Faculty of Medical Sciences 
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