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Publication year
2015Source
International Journal of Biochemistry & Cell Biology, 63, (2015), pp. 66-70ISSN
Publication type
Article / Letter to editor

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Organization
Paediatrics - OUD tm 2017
Biochemistry (UMC)
Pharmacology-Toxicology
Journal title
International Journal of Biochemistry & Cell Biology
Volume
vol. 63
Page start
p. 66
Page end
p. 70
Subject
Radboudumc 6: Metabolic Disorders RIMLS: Radboud Institute for Molecular Life SciencesAbstract
Mitochondria are double membrane organelles involved in various key cellular processes. Governed by dedicated protein machinery, mitochondria move and continuously fuse and divide. These "mitochondrial dynamics" are bi-directionally linked to mitochondrial and cell functional state in space and time. Due to the action of the electron transport chain (ETC), the mitochondrial inner membrane displays a inside-negative membrane potential (Deltapsi). The latter is considered a functional readout of mitochondrial "health" and required to sustain normal mitochondrial ATP production and mitochondrial fusion. During the last decade, live-cell microscopy strategies were developed for simultaneous quantification of Deltapsi and mitochondrial morphology. This revealed that ETC dysfunction, changes in Deltapsi and aberrations in mitochondrial structure often occur in parallel, suggesting they are linked potential targets for therapeutic intervention. Here we discuss how combining high-content and high-throughput strategies can be used for analysis of genetic and/or drug-induced effects at the level of individual organelles, cells and cell populations. This article is part of a Directed Issue entitled: Energy Metabolism Disorders and Therapies.
This item appears in the following Collection(s)
- Academic publications [204994]
- Electronic publications [103280]
- Faculty of Medical Sciences [81051]
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