Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine
SourceMethods in Molecular Biology, 1264, (2015), pp. 161-9
Article / Letter to editor
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Paediatrics - OUD tm 2017
Methods in Molecular Biology
SubjectRadboudumc 6: Metabolic Disorders RIMLS: Radboud Institute for Molecular Life Sciences
Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.
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