Publication year
2015Source
Archives of Oral Biology, 60, 3, (2015), pp. 501-7ISSN
Publication type
Article / Letter to editor
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Organization
Orthodontics and Oral Biology
Tumorimmunology
Dentistry
Journal title
Archives of Oral Biology
Volume
vol. 60
Issue
iss. 3
Page start
p. 501
Page end
p. 7
Subject
Radboudumc 10: Reconstructive and regenerative medicine RIMLS: Radboud Institute for Molecular Life Sciences; Radboudumc 17: Women's cancers RIMLS: Radboud Institute for Molecular Life SciencesAbstract
OBJECTIVE: Glycogen synthase kinase-3beta (Gsk-3beta)/beta-catenin signaling regulates development of the secondary palate. It has been unclear about the effects of Gsk-3beta/beta-catenin signaling on palatal fusion and osteogenic differentiation in palatal shelves. DESIGN: In this study, palatal shelves from mouse embryonic day 13 (E13) were cultured in vitro with or without lithium chloride (LiCl). Palatal fusion was evaluated by haematoxylin-eosin staining. The expression of osteogenic markers in palatal shelves was measured by quantitative PCR, and immunohistochemical staining. Cell proliferation and apoptosis were examined by Ki-67 immunohistochemical and TUNEL staining, respectively. Gsk-3beta expression was evaluated by quantitative PCR and Western blotting. beta-catenin protein expression was evaluated by Western blotting. RESULTS: After the treatment with 10 mM LiCl, palatal shelves failed to fuse, and the mRNA and protein levels of osteogenic markers were reduced compared with controls. The number of Ki67-positive cell in the palatal osteoid was significantly higher in the LiCl group than in the controls. The apoptotic cells in the midline epithelial seam were reduced by LiCl. Gsk-3beta mRNA and protein expression levels decreased and beta-catenin protein expression levels increased by treatment of LiCl. CONCLUSION: Our findings show that LiCl-mediated GSK3beta inhibition prevents palatal fusion and osteogenic differentiation in palatal shelves by increased beta-catenin signaling. It indicated that overactivation of canonical Wnt signaling might impair the fusion of the secondary palate.
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- Faculty of Medical Sciences [90358]
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