Soft tissue reaction to microgrooved poly-L-lactic acid implants loaded with transforming growth factor beta(3).
Publication year
2003Source
Tissue Engineering, 9, 1, (2003), pp. 117-126ISSN
Publication type
Article / Letter to editor

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Organization
Orthodontics and Oral Biology
Periodontology and Biomaterials
Journal title
Tissue Engineering
Volume
vol. 9
Issue
iss. 1
Page start
p. 117
Page end
p. 126
Subject
UMCN 4.3: Tissue engineering and reconstructive surgeryAbstract
In both normal and disturbed wound healing, the generation of large, contracting scars can raise serious functional and cosmetic problems. A possible strategy to minimize or avoid the generation of scar tissue surrounding an implant is to apply transforming growth factor-beta(3) (TGF-beta(3)) to the implant. TGF-beta(3) (0, 1, or 2.5 microg) was freeze-dried onto poly-L-lactic acid (PLA) microgrooved substrates (width, 10 microm; depth, 1 microm) and implanted subcutaneously on the backs of rats for 2 and 8 weeks. After sacrifice, implants and surrounding tissue were histologically processed. Light microscopic and histomorphometric evaluation of capsule thickness, capsule quality, and implant-tissue interface was performed. In addition, we stained for alpha-smooth muscle actin (SMA), collagen, and ED-1 (a monocyte-macrophage marker). All implants were surrounded by a fibrous capsule. Capsules of the implants loaded with 1 or 2.5 microg of TGF-beta(3) showed significantly higher capsule quality. This meant that capsules were more mature compared with implants without TGF-beta(3). However, no significant differences were found in terms of thickness of the capsules or quality of the interface. Finally, apparently significant differences were also found in the expression of alpha-SMA, when comparing the various growth factor concentrations at both implantation points. In conclusion, the use of microgrooved PLA substrates with TGF-beta(3) did not lead to an overall improvement of periimplant tissue healing.
This item appears in the following Collection(s)
- Academic publications [205105]
- Faculty of Medical Sciences [81055]
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