In vitro response to alkaline phosphatase coatings immobilized onto titanium implants using electrospray deposition or polydopamine-assisted deposition

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Publication year
2014Source
Journal of Biomedical Materials Research Part A, 102, 4, (2014), pp. 1102-9ISSN
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Article / Letter to editor

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Organization
Dentistry
Journal title
Journal of Biomedical Materials Research Part A
Volume
vol. 102
Issue
iss. 4
Page start
p. 1102
Page end
p. 9
Subject
Radboudumc 10: Reconstructive and regenerative medicine RIMLS: Radboud Institute for Molecular Life SciencesAbstract
Immobilization of biomolecules onto implant surfaces is one of the most straightforward strategies to control the interaction between an implant and its biological environment. Recently, it was shown that the enzyme alkaline phosphatase (ALP) could be efficiently immobilized onto titanium implants in a single step using polydopamine. We hypothesized that such polydopamine-ALP coatings can enhance the early attachment of cells and increase mineralization. Therefore, the current study aimed at immobilization of ALP onto titanium by means of either one- or two-step polydopamine-assisted immobilization or electrospray deposition, the comparative characterization of these experimental substrates and subsequent cell behavioral analysis using primary osteoblast-like cells. Uncoated titanium and ALP-free polydopamine coatings served as controls. Despite significant ALP surface activity and lower water contact for angles ALP-containing surface modifications, only marginal effects on early cell behavior (i.e., cell spreading) and osteogenic differentiation (i.e., proliferation, differentiation and mineralization) were observed in comparison to uncoated titanium. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1102-1109, 2014.
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- Faculty of Medical Sciences [86218]
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