Cloning, characterization, and mRNA expression analysis of novel human fetal cochlear cDNAs.

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Title:	Cloning, characterization, and mRNA expression analysis of novel human fetal cochlear cDNAs.
Author(s):	Luijendijk, M.W.J. ; Pol, T.J.R. van de; Duijnhoven, G.C.F. van; Hollander, A.I. den ; Caat, J. ten; Limpt, V. van; Brunner, H.G. ; Kremer, J.M.J. ; Cremers, F.P.M.
Publication year:	2003
Source:	Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, vol. 82, iss. 4, (2003), pp. 480-490
ISSN:	0888-7543
DOI:	http://dx.doi.org/10.1016/S0888-7543(03)00150-2
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/134330
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Subject:	UMCN 3.3: Neurosensory disorders UMCN 5.1: Genetic defects of metabolism
Organization:	Human Genetics Otorhinolaryngology
Journal title:	Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes
Volume:	vol. 82
Issue:	iss. 4
Page start:	p. 480
Page end:	p. 490
Abstract:	To identify novel genes that are expressed specifically or preferentially in the cochlea, we constructed a cDNA library enriched for human cochlear cDNAs using a suppression subtractive hybridization technique. We analyzed 2640 clones by sequencing and BLAST similarity searches. One hundred and fifty-five different cDNA fragments mapped in nonsyndromic hearing impairment loci for which the causative gene has not been cloned yet. Approximately 30% of the clones show no similarity to any known human gene or expressed sequence tag (EST). Clones mapping in nonsyndromic deafness loci and a selection of clones that represent novel ESTs were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) of RNA derived from 12 human fetal tissues. Our data suggest that a quarter of the novel genes in our library are preferentially expressed in fetal cochlea. These may play a physiologically important role in the hearing process and represent candidate genes for hereditary hearing impairment.