A molecular model for the synergic interaction between GABA and general anesthetics
SourceEuropean Journal of Pharmacology, 371, 2/3, (1999), pp. 213-226
Article / Letter to editor
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SW OZ DCC SMN
European Journal of Pharmacology
Within the context of the discussion about rational polytherapy, we determined the effects of four anaesthetics on the binding of [H-3]t-butylbicycloorthobenzoate ([H-3]TBOB) to the GABA(A) receptor complex in the presence of several concentrations of GABA (gamma-aminobutyric acid), in order to build a molecular model that can describe and quantify the interactions between the compounds. The empirical isobole method revealed that GABA and the anaesthetics acted synergically in displacing [H-3]TBOB. This synergy could be described by a simple molecular model in which both GABA and the anaesthetics displaced [H-3]TBOB allosterically and in which GABA allosterically enhanced the binding of the anaesthetics. To get information about the interaction between GABA and anaesthetics, we used [H-3]TBOB as a tracer ligand. The model indicated that GABA enhanced the affinity of thiopental 3.0-fold, propofol 5.0-fold, the neuroactive steroids Org 20599 3.5-fold and Org 20549 13-fold. Insight into the molecular mechanism and strength of these interactions can help clinicians to choose therapeutically optimal drug and dose combinations: a step towards rational polytherapy.
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