Studies towards the potential of poliovirus as a vector for the expression of HPV 16 virus-like-particles.

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Title:	Studies towards the potential of poliovirus as a vector for the expression of HPV 16 virus-like-particles.
Author(s):	Kuppeveld, F.J.M. van ; Jong, A.S. de ; Dijkman, H.B.P.M. ; Andino, R.; Melchers, W.J.G.
Publication year:	2002
Source:	FEMS Immunology and Medical Microbiology, vol. 34, iss. 3, (2002), pp. 201-208
ISSN:	0928-8244
DOI:	https://doi.org/10.1016/S0928-8244(02)00396-6
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/120709
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Subject:	Inflammatory reactions in the kidneys Pathogenesis, epidemiology, and treatment of microbial infections Immunologische ontstekingsprocessen in de nier Pathogenese, epidemiologie en behandeling van microbiële infecties
Organization:	Medical Microbiology Pathology
Journal title:	FEMS Immunology and Medical Microbiology
Volume:	vol. 34
Issue:	iss. 3
Page start:	p. 201
Page end:	p. 208
Classification:	BrainCell AgingImmunityKidney Anti-Infective Agents, QuinoloneAntibioticsAntifungal AgentsArthritis, InfectiousAspergillosisBacteria, AerobicBlood-Borne PathogensCandidiasisCross InfectionCytokinesFungemiaGram-Negative Aerobic BacteriaGram-Negative BacteriaGram-Negative Bacterial InfectionsLung Diseases, FungalLung Diseases, FungalMeningitis, FungalMolecular ProbesOpportunistic InfectionsOpportunistic InfectionsReiter's DiseaseSuperinfectionYeasts
Abstract:	Development of human cervical carcinomas is associated with infection by certain human papillomavirus (HPV) types. Thus, protection against HPV infection through vaccination may prevent development of cervical cancer. The purpose of this study was to investigate the possibility of using a poliovirus recombinant vector to induce immunity against HPV. A poliovirus recombinant was constructed which contained the complete coding sequence of the HPV 16 major capsid protein L1, between the P1 and P2 region of the poliovirus polyprotein. A replication-competent virus was obtained after transfection of the recombinant RNA into tissue culture cells. Electron microscopically examination of cells infected with the poliovirus-HPV L1 recombinant indicated that HPV 16 L1 self-assembles into virus-like particles. To investigate the immunological response in vivo, susceptible transgenic mice carrying the poliovirus receptor were infected with the recombinant poliovirus. In all mice a modest but consistent immune response against HPV 16 was observed. Based on these results, the potential for picornavirus-derived vectors in vaccine development against HPV infection is discussed.