Gateway to understanding microparticles: standardized isolation and identification of plasma membrane-derived vesicles

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Title:	Gateway to understanding microparticles: standardized isolation and identification of plasma membrane-derived vesicles
Author(s):	Dinkla, S. ; Brock, R.E. ; Joosten, I. ; Bosman, G.J.C.G.M.
Publication year:	2013
Source:	Nanomedicine, vol. 8, iss. 10, (2013), pp. 1657-1668
ISSN:	1743-5889
DOI:	http://dx.doi.org/10.2217/NNM.13.149
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/119282
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Subject:	N4i 4: Auto-immunity, transplantation and immunotherapy N4i 4: Auto-immunity, transplantation and immunotherapy NCMLS 2: Immune Regulation NCMLS 7: Chemical and physical biology
Organization:	Laboratory of Medical Immunology Biochemistry (UMC)
Journal title:	Nanomedicine
Volume:	vol. 8
Issue:	iss. 10
Page start:	p. 1657
Page end:	p. 1668
Abstract:	Microparticles (MPs) are small plasma membrane-derived vesicles that can expose molecules originating from their parental cells. As vectors of biological information they are likely to play an active role in both homeostasis and pathogenesis, making them promising biomarkers and nanomedicine tools. Therefore, there is an urgent need for standardization of MP isolation and analysis protocols to propel our understanding of MP biology to the next level. Based on current methodology and recent insights, this review proposes an optimized protocol for the isolation and biochemical characterization of MPs.