Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
Publication year
2013Source
PLoS One, 8, 8, (2013), article e72610ISSN
Publication type
Article / Letter to editor

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Organization
Dentistry
Orthopaedics
Journal title
PLoS One
Volume
vol. 8
Issue
iss. 8
Subject
NCMLS 3: Tissue engineering and pathologyAbstract
The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the sustained release of bone morphogenetic protein 2 (BMP-2) influences osteogenicity of tissue engineered bioprinted constructs. BMP-2 loaded on gelatin microparticles (GMPs) was used as a sustained release system, which was dispersed in hydrogel-based constructs and compared to direct inclusion of BMP-2 in alginate or control GMPs. The constructs were supplemented with goat multipotent stromal cells (gMSCs) and biphasic calcium phosphate to study osteogenic differentiation and bone formation respectively. BMP-2 release kinetics and bioactivity showed continuous release for three weeks coinciding with osteogenicity. Osteogenic differentiation and bone formation of bioprinted GMP containing constructs were investigated after subcutaneous implantation in mice or rats. BMP-2 significantly increased bone formation, which was not influenced by the release timing. We showed that 3D printing of controlled release particles is feasible and that the released BMP-2 directs osteogenic differentiation in vitro and in vivo.
This item appears in the following Collection(s)
- Academic publications [202863]
- Electronic publications [101052]
- Faculty of Medical Sciences [80039]
- Open Access publications [69734]
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