Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa

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Title:	Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa
Author(s):	Erkek, S.; Hisano, M.; Liang, C.Y.; Gill, M.; Murr, R.; Dieker, J.W.C. ; Schubeler, D.; Vlag, J. van der ; Stadler, M.B.; Peters, A.H.
Publication year:	2013
Source:	Nature Structural & Molecular Biology, vol. 20, iss. 7, (2013), pp. 868-875
ISSN:	1545-9993
DOI:	https://doi.org/10.1038/nsmb.2599
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/119148
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Subject:	NCMLS 3: Tissue engineering and pathology N4i 4: Auto-immunity, transplantation and immunotherapy NCMLS 7: Chemical and physical biology
Organization:	Biochemistry (UMC) Nephrology
Journal title:	Nature Structural & Molecular Biology
Volume:	vol. 20
Issue:	iss. 7
Page start:	p. 868
Page end:	p. 875
Abstract:	In mammalian spermatozoa, most but not all of the genome is densely packaged by protamines. Here we reveal the molecular logic underlying the retention of nucleosomes in mouse spermatozoa, which contain only 1% residual histones. We observe high enrichment throughout the genome of nucleosomes at CpG-rich sequences that lack DNA methylation. Residual nucleosomes are largely composed of the histone H3.3 variant and are trimethylated at Lys4 of histone H3 (H3K4me3). Canonical H3.1 and H3.2 histones are also enriched at CpG-rich promoters marked by Polycomb-mediated H3K27me3, a modification predictive of gene repression in preimplantation embryos. Histone variant-specific nucleosome retention in sperm is strongly associated with nucleosome turnover in round spermatids. Our data show evolutionary conservation of the basic principles of nucleosome retention in mouse and human sperm, supporting a model of epigenetic inheritance by nucleosomes between generations.