Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation
Publication year
2013Source
Journal of Biological Chemistry, 288, 8, (2013), pp. 5530-8ISSN
Publication type
Article / Letter to editor
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Organization
Biochemistry (UMC)
Journal title
Journal of Biological Chemistry
Volume
vol. 288
Issue
iss. 8
Page start
p. 5530
Page end
p. 8
Subject
NCMLS 3: Tissue engineering and pathologyAbstract
As our understanding of what guides the behavior of multi- and pluripotent stem cells deepens, so too does our ability to utilize certain cues to manipulate their behavior and maximize their therapeutic potential. Engineered, biologically functionalized materials have the capacity to influence stem cell behavior through a powerful combination of biological, mechanical, and topographical cues. Here, we present the development of a novel electrospun scaffold, functionalized with glycosaminoglycans (GAGs) ionically immobilized onto the fiber surface. Bound GAGs retained the ability to interact with GAG-binding molecules and, crucially, presented GAG sulfation motifs fundamental to mediating stem cell behavior. Bound GAG proved to be biologically active, rescuing the neural differentiation capacity of heparan sulfate-deficient mouse embryonic stem cells and functioning in concert with FGF4 to facilitate the formation of extensive neural processes across the scaffold surface. The combination of GAGs with electrospun scaffolds creates a biomaterial with potent applicability for the propagation and effective differentiation of pluripotent stem cells.
This item appears in the following Collection(s)
- Academic publications [246860]
- Electronic publications [134253]
- Faculty of Medical Sciences [93474]
- Open Access publications [107774]
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