The transition of mouse pluripotent stem cells from the naive to the primed state requires Fas signaling through 3-O sulfated heparan sulfate structures recognized by the HS4C3 antibody
Publication year
2013Source
Biochemical and Biophysical Research Communications, 430, 3, (2013), pp. 1175-81ISSN
Publication type
Article / Letter to editor
Display more detailsDisplay less details
Organization
Biochemistry (UMC)
Journal title
Biochemical and Biophysical Research Communications
Volume
vol. 430
Issue
iss. 3
Page start
p. 1175
Page end
p. 81
Subject
NCMLS 3: Tissue engineering and pathologyAbstract
The characteristics of pluripotent embryonic stem cells of human and mouse are different. The properties of human embryonic stem cells (hESCs) are similar to those of mouse epiblast stem cells (mEpiSCs), which are in a later developmental pluripotency state, the so-called "primed state" compared to mouse embryonic stem cells (mESCs) which are in a naive state. As a result of the properties of the primed state, hESCs proliferate slowly, cannot survive as single cells, and can only be transfected with genes at low efficiency. Generating hESCs in the naive state is necessary to overcome these problems and allow their application in regenerative medicine. Therefore, clarifying the mechanism of the transition between the naive and primed states in pluripotent stem cells is important for the establishment of stable methods of generating naive state hESCs. However, the signaling pathways which contribute to the transition between the naive and primed states are still unclear. In this study, we carried out induction from mESCs to mEpiSC-like cells (mEpiSCLCs), and observed an increase in the activation of Fas signaling during the induction. The expression of Fgf5, an epiblast marker, was diminished by inhibition of Fas signaling using the caspase-8 and -3 blocking peptides, IETD and DEVD, respectively. Furthermore, during the induction, we observed increased expression of 3-O sulfated heparan sulfate (HS) structures synthesized by HS 3-O-sulfotransferase (3OST), which are recognized by the HS4C3 antibody (HS4C3-binding epitope). Knockdown of 3OST-5 reduced Fas signaling and the potential for the transition to mEpiSCLCs. This indicates that the HS4C3-binding epitope is necessary for the transition to the primed state. We propose that Fas signaling through the HS4C3-binding epitope contributes to the transition from the naive state to the primed state.
This item appears in the following Collection(s)
- Academic publications [246625]
- Faculty of Medical Sciences [93367]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.