Bacterial responses to a simulated colon tumor microenvironment
SourceMolecular & Cellular Proteomics, 11, 10, (2012), pp. 851-862
Article / Letter to editor
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Laboratory of Genetic, Endocrine and Metabolic Diseases
Paediatrics - OUD tm 2017
Molecular & Cellular Proteomics
SubjectIGMD 3: Genomic disorders and inherited multi-system disorders; IGMD 7: Iron metabolism N4i 1: Pathogenesis and modulation of inflammation; IGMD 7: Iron metabolism ONCOL 5: Aetiology, screening and detection; N4i 1 - pathogenesis and modulation of inflammation Oncol 5 - Aetiology, screening and detection; NCMLS 4: Energy and redox metabolism IGMD 8: Mitochondrial medicine; NCMLS 5: Membrane transport and intracellular motility IGMD 9: Renal disorder
One of the few bacteria that have been consistently linked to colorectal cancer (CRC) is the opportunistic pathogen Streptococcus gallolyticus. Infections with this bacterium are generally regarded as an indicator for colonic malignancy, while the carriage rate of this bacterium in the healthy large intestine is relatively low. We speculated that the physiological changes accompanying the development of CRC might favor the colonization of this bacterium. To investigate whether colon tumor cells can support the survival of S. gallolyticus, this bacterium was grown in spent medium of malignant colonocytes to simulate the altered metabolic conditions in the CRC microenvironment. These in vitro simulations indicated that S. gallolyticus had a significant growth advantage in these spent media, which was not observed for other intestinal bacteria. Under these conditions, bacterial responses were profiled by proteome analysis and metabolic shifts were analyzed by (1)H-NMR-spectroscopy. In silico pathway analysis of the differentially expressed proteins and metabolite analysis indicated that this advantage resulted from the increased utilization of glucose, glucose derivates, and alanine. Together, these data suggest that tumor cell metabolites facilitate the survival of S. gallolyticus, favoring its local outgrowth and providing a possible explanation for the specific association of S. gallolyticus with colonic malignancy.
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