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Title: Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
Author(s): Vodovar, N.; Bronkhorst, A.W.; Cleef, K.W.R. van; Miesen, P.; Blanc, H.; Rij, R.P. van ; Saleh, M.C.
Publication year: 2012
Source: PLoS One, vol. 7, iss. 1, (2012), pp. e30861
ISSN: 1932-6203
DOI: http://dx.doi.org/10.1371/journal.pone.0030861
Publication type: Article / Letter to editor

Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/109736   http://hdl.handle.net/2066/109736

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Subject: N4i 1: Pathogenesis and modulation of inflammation NCMLS 1: Infection and autoimmunity
Organization: Medical Microbiology
Journal title:
PLoS One
Volume: vol. 7
Issue: iss. 1
Page start: p. e30861
Page end: p. e30861
Abstract:
The siRNA pathway is an essential antiviral mechanism in insects. Whether other RNA interference pathways are involved in antiviral defense remains unclear. Here, we report in cells derived from the two main vectors for arboviruses, Aedes albopictus and Aedes aegypti, the production of viral small RNAs that exhibit the hallmarks of ping-pong derived piwi-associated RNAs (piRNAs) after infection with positive or negative sense RNA viruses. Furthermore, these cells produce endogenous piRNAs that mapped to transposable elements. Our results show that these mosquito cells can initiate de novo piRNA production and recapitulate the ping-pong dependent piRNA pathway upon viral infection. The mechanism of viral-piRNA production is discussed.

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