Resveratrol prevents nicotine-induced teratogenesis in cultured mouse embryos

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Title:	Resveratrol prevents nicotine-induced teratogenesis in cultured mouse embryos
Author(s):	Lin, C.; Yon, J.M.; Jung, A.Y. ; Lee, J.G.; Jung, K.Y.; Kang, J.K.; Lee, B.J.; Yun, Y.W.; Nam, S.Y.
Publication year:	2012
Source:	Reproductive Toxicology, vol. 34, iss. 3, (2012), pp. 340-346
ISSN:	0890-6238
DOI:	http://dx.doi.org/10.1016/j.reprotox.2012.05.097
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : http://hdl.handle.net/2066/109391
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Subject:	NCEBP 1: Molecular epidemiology ONCOL 5: Aetiology, screening and detection
Organization:	Health Evidence
Former Organization:	Epidemiology, Biostatistics & HTA
Journal title:	Reproductive Toxicology
Volume:	vol. 34
Issue:	iss. 3
Page start:	p. 340
Page end:	p. 346
Abstract:	Nicotine, a major toxic component in tobacco smoke, leads to severe embryonic damage during organogenesis in embryos. We investigated whether resveratrol would positively influence nicotine-induced teratogenesis in mouse embryos (embryonic day 8.5) cultured for 48 h using a whole embryo culture system. Embryos exposed to nicotine (1mM) revealed significantly severe morphological anomalies, increased levels of caspase-3 mRNA and lipid peroxidation, and decreased levels of cytoplasmic superoxide dismutase (SOD), mitochondrial manganese SOD, cytosolic glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, hypoxia-inducible factor 1alpha, Bcl-x(L), and sirtuin1 (SIRT1) mRNAs and SOD activity compared to those in the normal control group. However, when resveratrol (1x10(-8) muM or 1x10(-7) muM) was added concurrently to the embryos exposed to nicotine, all the parameters in above improved conspicuously. These findings indicate that resveratrol has a noted protective effect against nicotine-induced teratogenesis in mouse embryos through its antioxidative and anti-apoptotic effects.