Regulation of TRIB3 mRNA and Protein in Breast Cancer
Publication year
2012Source
PLoS One, 7, 11, (2012), article e49439ISSN
Publication type
Article / Letter to editor

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Organization
Laboratory of Genetic, Endocrine and Metabolic Diseases
Radiation Oncology
Journal title
PLoS One
Volume
vol. 7
Issue
iss. 11
Subject
IGMD 6: Hormonal regulation ONCOL 5: Aetiology, screening and detection; ONCOL 3: Translational researchAbstract
Tribbles homolog 3 (TRIB3) is a scaffold protein activated under hypoxic conditions and involved in several cell survival and proliferation pathways. Recently, we reported opposite associations of TRIB3 mRNA and protein with breast cancer prognosis. In this study, we investigated this discrepancy between TRIB3 mRNA and protein in human breast cancer. We provide several lines of evidence demonstrating that TRIB3 is a stabile protein which levels are not controlled by rapid protein breakdown. Interestingly, we were able to show that during anoxia TRIB3 mRNA translation was profoundly inhibited. Hypoxia induced micro RNA 24 was not responsible for the translational repression of TRIB3. Furthermore miRNA-24 expression levels in breast cancer patient specimens showed no correlation with TRIB3 mRNA or TRIB3 protein levels, or with prognosis. Thus, the expression of miRNA-24 does not explain the difference between mRNA and protein expression of TRIB3 in this cohort of breast cancer patients. In conclusion, TRIB3 protein is a stable protein which levels are predominantly regulated by translational control of TRIB3 mRNA transcript.
This item appears in the following Collection(s)
- Academic publications [204980]
- Electronic publications [103240]
- Faculty of Medical Sciences [81051]
- Open Access publications [71779]
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