Protein enrichment by capture-release based on strain-promoted cycloaddition of azide with bicyclononyne (BCN).
until further notice
SourceBioorganic & Medicinal Chemistry, 20, 2, (2012), pp. 655-661
Article / Letter to editor
Display more detailsDisplay less details
Synthetic Organic Chemistry
Laboratory of Genetic, Endocrine and Metabolic Diseases
Physical Organic Chemistry
Bioorganic & Medicinal Chemistry
SubjectDCN NN - Brain networks and neuronal communication; DCN PAC - Perception action and control IGMD 4: Glycostation disorders; IGMD 8: Mitochondrial medicine; Synthetic Organic Chemistry
An enrichment strategy was devised for azide derivatized macromolecules, based on strain-promoted alkyne-azide cycloaddition (SPAAC) and a cleavable linker. A ring-strained alkyne, bicyclo[6.1.0]non-4-yne (BCN), was covalently attached to agarose beads via a hydrazine-sensitive linker. Benchmark studies of the resulting 'azido-trap' beads were performed with a fluorogenic coumarin derivative, leading to efficient capture of the azidocoumarin with concomitant fluorescence staining of the beads via SPAAC. The versatility of the beads for specific protein enrichment was shown by an effective and highly specific capture-release strategy for enrichment of azido-containing Candida antarctica lipase B (CalB) from a mixture of proteins. This approach is suited for selective enrichment of (glyco)proteins after metabolic incorporation of azides for subsequent (glyco)proteomics studies.
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.