Abstract
AIM: To evaluate the effect of alkaline phosphatase (ALP) immobilization onto Bio-Gide((R)) in vitro, and to study the in vivo performance of ALP-enriched Bio-Gide((R)) and/or Bio-Oss((R)) with the purpose to enhance periodontal regeneration. MATERIALS AND METHODS: Alkaline phosphatase ALP was immobilized onto Bio-Gide((R)) and Bio-Oss((R)) . Forty-eight rats received periodontal defects, which were treated according to one of the following strategies: Bio-Gide((R)), Bio-Gide((R)) -ALP, Bio-Gide((R)) -ALP/Bio-Oss((R)), Bio-Gide((R)) /Bio-Oss((R)) -ALP, Bio-Gide((R)) -ALP/Bio-Oss((R)) -ALP, or empty. Micro-CT and histological analysis were performed. RESULTS: A 30 min ALP-deposition time was determined as optimal from mineralization capacity assessment and consequently used as Bio-Gide((R)) -ALP membranes in the animal experiment. In vivo results showed that after 2 weeks, the defect and implanted materials were still visible, an inflammatory response was present, and membrane degradation was ongoing. Bone formation, although limited, was observed in the majority of Bio-Gide((R)) -ALP specimens and all of the Bio-Gide((R)) /Bio-Oss((R)) -ALP specimens, and was significantly higher compared with Bio-Gide((R)) and empty controls. After 6 weeks, the defects and particles were still visible, whereas membranes were completely degraded. The inflammatory response was decreased and bone formation appeared superior for Bio-Gide((R)) -ALP treated defects. CONCLUSION: Immobilization of ALP onto guided tissue regeneration (GTR)/ guided bone regeneration (GBR)-materials (Bio-Gide((R)) and Bio-Oss((R))) can enhance the performance of these materials in GTR/GBR procedures.
