High detection rate of clinically relevant genomic abnormalities in plasma cells enriched from patients with multiple myeloma
Publication year
2012Source
Genes, Chromosomes & Cancer, 51, 11, (2012), pp. 997-1006ISSN
Publication type
Article / Letter to editor
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Organization
Haematology
Human Genetics
Journal title
Genes, Chromosomes & Cancer
Volume
vol. 51
Issue
iss. 11
Page start
p. 997
Page end
p. 1006
Subject
NCMLS 6: Genetics and epigenetic pathways of disease ONCOL 3: Translational researchAbstract
Multiple myeloma is a heterogeneous disease, which is characterized by the occurrence of specific genomic abnormalities that are both of diagnostic and prognostic relevance. Since the detection of these abnormalities through molecular-genetic techniques is hampered by the overall low percentage of plasma cells present in primary bone marrow aspirates, we assessed the efficacy of these techniques in enriched plasma cell fractions from 61 multiple myeloma patients. Using interphase FISH, genomic abnormalities could be detected in 96% of the enriched samples as compared to 61% in the cultured whole bone marrow samples. We also found that microarray-based genomic profiling of enriched plasma samples facilitates the detection of additional, possibly clinically relevant, genomic abnormalities. We conclude that the genomic delineation of enriched plasma cells from multiple myeloma patients results in a significantly increased detection rate of clinically relevant genomic abnormalities. In order to facilitate molecular-genetic data interpretation, we recommend morphological assessment of plasma cell purity after enrichment. (c) 2012 Wiley Periodicals, Inc.
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- Faculty of Medical Sciences [92893]
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