Genetic variation in T-box binding element functionally affects SCN5A/SCN10A enhancer.
Publication year
2012Source
Journal of Clinical Investigation, 122, 7, (2012), pp. 2519-30ISSN
Publication type
Article / Letter to editor
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Organization
Intensive Care
Journal title
Journal of Clinical Investigation
Volume
vol. 122
Issue
iss. 7
Page start
p. 2519
Page end
p. 30
Subject
N4i 1: Pathogenesis and modulation of inflammation NCEBP 6: Quality of nursing and allied health careAbstract
The contraction pattern of the heart relies on the activation and conduction of the electrical impulse. Perturbations of cardiac conduction have been associated with congenital and acquired arrhythmias as well as cardiac arrest. The pattern of conduction depends on the regulation of heterogeneous gene expression by key transcription factors and transcriptional enhancers. Here, we assessed the genome-wide occupation of conduction system-regulating transcription factors TBX3, NKX2-5, and GATA4 and of enhancer-associated coactivator p300 in the mouse heart, uncovering cardiac enhancers throughout the genome. Many of the enhancers colocalized with ion channel genes repressed by TBX3, including the clustered sodium channel genes Scn5a, essential for cardiac function, and Scn10a. We identified 2 enhancers in the Scn5a/Scn10a locus, which were regulated by TBX3 and its family member and activator, TBX5, and are functionally conserved in humans. We also provided evidence that a SNP in the SCN10A enhancer associated with alterations in cardiac conduction patterns in humans disrupts TBX3/TBX5 binding and reduces the cardiac activity of the enhancer in vivo. Thus, the identification of key regulatory elements for cardiac conduction helps to explain how genetic variants in noncoding regulatory DNA sequences influence the regulation of cardiac conduction and the predisposition for cardiac arrhythmias.
This item appears in the following Collection(s)
- Academic publications [246165]
- Electronic publications [133717]
- Faculty of Medical Sciences [93268]
- Open Access publications [107229]
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