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Title: Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.
Author(s): Hurst, S.F.; Kidd, S.E.; Morrissey, C.O.; Snelders, E. ; Melchers, W.J.G. ; Castelli, M.V.; Mellado, E.; Simmon, K.; Petti, C.A.; Richardson, S.; Zhang, S.; Romanelli, A.M.; Wickes, B.L.; Valk, H.A. de; Klaassen, C.H.; Balajee, S.A.
Publication year: 2009
Source: Journal of Clinical Microbiology, vol. 47, iss. 5, (2009), pp. 1562-1564
ISSN: 0095-1137
DOI: https://doi.org/10.1128/JCM.00124-09
Publication type: Article / Letter to editor

Please use this identifier to cite or link to this item : https://hdl.handle.net/2066/80126   https://hdl.handle.net/2066/80126

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Subject: N4i 2: Invasive mycoses and compromised host
NCMLS 1: Infection and autoimmunity
Organization: Medical Microbiology
Journal title:
Journal of Clinical Microbiology
Volume: vol. 47
Issue: iss. 5
Page start: p. 1562
Page end: p. 1564
Abstract:
Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

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