Amplified fragment length polymorphism fingerprinting is an effective technique to distinguish streptococcus pneumoniae from other Streptococci and an efficient alternative to pulsed-field gel electrophoresis for molecular typing of pneumococci.

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Title:	Amplified fragment length polymorphism fingerprinting is an effective technique to distinguish streptococcus pneumoniae from other Streptococci and an efficient alternative to pulsed-field gel electrophoresis for molecular typing of pneumococci.
Author(s):	Neeleman, C. ; Klaassen, C.H.W.; Valk, H.A. de; Ruiter, M.T. de; Mouton †, J.W.
Publication year:	2004
Source:	Journal of Clinical Microbiology, vol. 42, iss. 1, (2004), pp. 369-371
ISSN:	0095-1137
DOI:	https://doi.org/10.1128/JCM.42.1.369-371.2004
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : https://hdl.handle.net/2066/58092
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Subject:	UMCN 4.1: Microbial pathogenesis and host defense
Organization:	Intensive Care Biochemistry (UMC) Medical Microbiology
Journal title:	Journal of Clinical Microbiology
Volume:	vol. 42
Issue:	iss. 1
Page start:	p. 369
Page end:	p. 371
Abstract:	Amplified fragment length polymorphism versus pulsed-field gel electrophoresis was used for fingerprinting of 85 macrolide-resistant pneumococcal isolates identified by using primarily phenotypic methods. Confirmation of identification by 16S rRNA sequencing revealed that 27 isolates were actually nonpneumococci. Amplified fragment length polymorphism but not pulsed-field gel electrophoresis offered simultaneous and accurate discrimination between pneumococci and nonpneumococcal species.