Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

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Title:	Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells
Author(s):	Nagelkerke, A.P.; Span, P.N.
Publication year:	2016
Source:	Advances in Experimental Medicine and Biology, vol. 899, (2016), pp. 1-10
ISSN:	0065-2598
DOI:	http://dx.doi.org/10.1007/978-3-319-26666-4_1
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : https://hdl.handle.net/2066/171535
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Subject:	Radboudumc 17: Women's cancers RIHS: Radboud Institute for Health Sciences
Organization:	Laboratory Medicine Radiation Oncology
Journal title:	Advances in Experimental Medicine and Biology
Volume:	vol. 899
Page start:	p. 1
Page end:	p. 10
Abstract:	Phospho-H2AX or gamma-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against gamma-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.