The E1/E2-preference of gastric H,K-ATPase mutants.

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Title:	The E1/E2-preference of gastric H,K-ATPase mutants.
Author(s):	Pont, J.J.H.H.M. de ; Swarts, H.G.P. ; Willems, P.H.G.M. ; Koenderink, J.B.
Publication year:	2003
Source:	Annals of the New York Academy of Sciences, vol. 986, (2003), pp. 175-182
ISSN:	0077-8923
Publication type:	Article / Letter to editor
Please use this identifier to cite or link to this item : https://hdl.handle.net/2066/142699
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Subject:	UMCN 5.4: Renal disorders
Organization:	Biochemistry (UMC)
Journal title:	Annals of the New York Academy of Sciences
Volume:	vol. 986
Page start:	p. 175
Page end:	p. 182
Abstract:	Gastric H,K-ATPase has, in the absence of ATP and added ions, a preference for the E(2) conformation. Mutations in the cation-binding pocket often result in a preference for the E(1)-conformation. This can be paralleled by the occurrence of K(+)-independent ATPase activity. These two phenomena could be separated by combined mutagenesis of several residues in and around the cation-binding pocket. Models of the three-dimensional structure of H,K-ATPase visualize the relationship between the E(1)/E(2) preference and the structure.