TY - JOUR AU - Majumder, P.P. AU - Mhlanga, M.M.K. AU - Shalek, A.K. PY - 2020 UR - https://hdl.handle.net/2066/227598 TI - The Human Cell Atlas and equity: lessons learned EP - 1511 SN - 1078-8956 SP - 1509 JF - Nature Medicine VL - vol. 26 PS - 3 p. DO - https://doi.org/10.1038/s41591-020-1100-4 ER - TY - JOUR AU - Bruijn, S.E. de AU - Fiorentino, Alessia AU - Ottaviani, Daniele AU - Fanucchi, Stephanie AU - Melo, Uira S. AU - Corral-Serrano, J.C. AU - Mulders, T.W.F. AU - Albert, S. AU - Gilissen, C.F. AU - Aben, M.J. AU - Corominas, J. AU - Kremer, H. AU - Hoyng, C.B. AU - Mhlanga, M.M.K. AU - Cremers, F.P.M. AU - Roosing, S. AU - Hardcastle, Alison J. PY - 2020 UR - https://hdl.handle.net/2066/227412 TI - Structural Variants Create New Topological-Associated Domains and Ectopic Retinal Enhancer-Gene Contact in Dominant Retinitis Pigmentosa EP - 814 SN - 0002-9297 IS - iss. 5 SP - 802 JF - American Journal of Human Genetics VL - vol. 107 DO - https://doi.org/10.1016/j.ajhg.2020.09.002 ER - TY - JOUR AU - Moorlag, S.J.C.F.M. AU - Rodriguez Rosales, Y.A. AU - Gillard, J.J. AU - Fanucchi, Stephanie AU - Theunissen, K. AU - Novakovic, Boris AU - Bont, C.M. de AU - Fok, E.T. AU - Mourits, V.P. AU - Koeken, V.A.C.M. AU - Bree, L.C.J. de AU - Pruijn, G.J.M. AU - Crevel, R. van AU - Joosten, L.A.B. AU - Joosten, I. AU - Mhlanga, M.M.K. AU - Diavatopoulos, D.A. AU - Chavakis, T. AU - Netea, M.G. PY - 2020 UR - https://hdl.handle.net/2066/227444 TI - BCG Vaccination Induces Long-Term Functional Reprogramming of Human Neutrophils SN - 2211-1247 IS - iss. 7 JF - Cell Reports VL - vol. 33 DO - https://doi.org/10.1016/j.celrep.2020.108387 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/227444/227444.pdf?sequence=1 ER - TY - JOUR AU - Prange, K.H.M. AU - Mandoli, A. AU - Kuznetsova, T. AU - Wang, S. AU - Sotoca, A.M. AU - Marneth, A.E. AU - Van der Reijden, B.A. AU - Stunnenberg, H. AU - Martens, J.H.A. PY - 2017 UR - https://hdl.handle.net/2066/168892 TI - MLL-AF9 and MLL-AF4 oncofusion proteins bind a distinct enhancer repertoire and target the RUNX1 program in 11q23 acute myeloid leukemia EP - 11 SN - 0950-9232 SP - 1 JF - Oncogene PS - 11 p. DO - https://doi.org/10.1038/onc.2016.488 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/168892/168892.pdf?sequence=1 ER - TY - JOUR AU - van den Boom, V. AU - Maat, H. AU - Geugien, M. AU - Lopez, A.R. AU - Sotoca, A.M. AU - Jaques, J. AU - Brouwers-Vos, A.Z. AU - Fusetti, F. AU - Groen, R.W.J. AU - Yuan, H.P. AU - Martens, A.C.M. AU - Stunnenberg, H.G. AU - Vellenga, E. AU - Martens, J.H.A. AU - Schuringa, J.J. PY - 2016 UR - https://hdl.handle.net/2066/156956 TI - Non-canonical PRC1.1 Targets Active Genes Independent of H3K27me3 and Is Essential for Leukemogenesis EP - 346 SN - 2211-1247 IS - iss. 2 SP - 332 JF - Cell Reports VL - vol. 14 DO - https://doi.org/10.1016/j.celrep.2015.12.034 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/156956/156956.pdf?sequence=1 ER - TY - GEN AU - Zoelen, E.J.J. van PY - 2016 UR - https://hdl.handle.net/2066/162611 PB - Nijmegen : Radboud Universiteit TI - Celbiologie, een bewuste keuze? N1 - Rede uitgesproken bij het afscheid als hoogleraar Celbiologie aan de Faculteit der Natuurwetenschappen, Wiskunde en Informatica van de Radboud Universiteit, 18 november 2016 PS - 27 p. ER - TY - JOUR AU - Sotoca Covaleda, A.M. AU - Prange, K.H.M. AU - Reijnders, B. AU - Mandoli, A. AU - Nguyen, L.N. AU - Stunnenberg, H.G. AU - Martens, J.H.A. PY - 2015 UR - https://hdl.handle.net/2066/145003 TI - The oncofusion protein FUS-ERG targets key hematopoietic regulators and modulates the all-trans retinoic acid signaling pathway in t(16;21) acute myeloid leukemia EP - 1976 SN - 0950-9232 SP - 1965 JF - Oncogene VL - vol. 35 DO - https://doi.org/10.1038/onc.2015.261 ER - TY - JOUR AU - Oliveira, J.M.P.F. de AU - Oliveira, H. AU - Pinho, S. AU - Pimentel, F. AU - Almeida, L. AU - Zoelen, E.J.J. van AU - Santos, C. dos PY - 2014 UR - https://hdl.handle.net/2066/135376 TI - Cytotoxic and genotoxic activity of fisetin (3, 3 ', 4 ', 7-tetrahydroxyflavone) in an osteosarcoma in vitro model EP - 1412 SN - 0032-0943 IS - iss. 16 SP - 1412 JF - Planta Medica VL - vol. 80 ER - TY - JOUR AU - Oliveira, J.M.P.F. de AU - Almeida, L. AU - Pimentel, F. AU - Zoelen, E.J.J. van AU - Santos, C. dos PY - 2014 UR - https://hdl.handle.net/2066/135421 TI - Analysis of stably expressed genes with low-dose etoposide for toxicological studies in osteosarcoma EP - 1452 SN - 0032-0943 IS - iss. 16 SP - 1451 JF - Planta Medica VL - vol. 80 ER - TY - THES AU - Meijer, I.M.J. PY - 2014 SN - 97890902 UR - https://hdl.handle.net/2066/122889 PB - [S.l. : s.n.] TI - Ubiquitination and deubiquitination of ERBB receptors N1 - Radboud Universiteit Nijmegen, 24 januari 2014 N1 - Promotor : Zoelen, E.J.J. van Co-promotor : Leeuwen, J.E.M. van PS - 223 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/122889/122889.pdf?sequence=1 ER - TY - JOUR AU - Hensen, S.M.M. AU - Heldens, L. AU - Enckevort, C.M.W. van AU - Genesen, S.T. van AU - Pruijn, G.J.M. AU - Lubsen, N.H. PY - 2013 UR - https://hdl.handle.net/2066/111502 TI - Activation of the antioxidant response in methionine deprived human cells results in an HSF1-independent increase in HSPA1A mRNA levels EP - 1251 SN - 0300-9084 IS - iss. 6 SP - 1245 JF - Biochimie VL - vol. 95 DO - http://dx.doi.org/10.1016/j.biochi.2013.01.017 ER - TY - JOUR AU - Hensen, S.M. AU - Heldens, L. AU - Genesen, S.T. van AU - Pruijn, G.J.M. AU - Lubsen, N.H. PY - 2013 UR - https://hdl.handle.net/2066/122421 TI - A delayed antioxidant response in heat-stressed cells expressing a non-DNA binding HSF1 mutant EP - 473 SN - 1355-8145 IS - iss. 4 SP - 455 JF - Cell Stress & Chaperones VL - vol. 18 N1 - 10 juni 2013 DO - http://dx.doi.org/10.1007/s12192-012-0400-0 ER - TY - JOUR AU - Blok, E.J. AU - Kuppen, P.J. AU - Leeuwen, J.E. van AU - Sier, C.F. PY - 2013 UR - https://hdl.handle.net/2066/111474 TI - Cytoplasmic Overexpression of HER2: a Key Factor in Colorectal Cancer EP - 51 SN - 1179-5549 SP - 41 JF - Clinical Medicine Insights: Oncology VL - vol. 7 DO - http://dx.doi.org/10.4137/cmo.s10811 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/111474/111474.pdf?sequence=1 ER - TY - JOUR AU - Rietjens, I.M.C.M. AU - Sotoca Covaleda, A.M. AU - Vervoort, J. AU - Louisse, J. PY - 2013 UR - https://hdl.handle.net/2066/111537 TI - Mechanisms underlying the dualistic mode of action of major soy isoflavones in relation to cell proliferation and cancer risks EP - 113 SN - 1613-4125 IS - iss. 1 SP - 100 JF - Molecular Nutrition & Food Research (Formerly Nahrung/Food) VL - vol. 57 DO - https://doi.org/10.1002/mnfr.201200439 ER - TY - JOUR AU - Meijer, I.M.J. AU - Rotterdam, W. van AU - Zoelen, E.J.J. van AU - Leeuwen, J.E.M. van PY - 2013 UR - https://hdl.handle.net/2066/103221 TI - Cbl and Itch binding sites in ERBB4 CYT-1 and CYT-2 mediate K48- and K63-polyubiquitination, respectively EP - 478 SN - 0898-6568 IS - iss. 2 SP - 470 JF - Cellular Signalling VL - vol. 25 DO - https://doi.org/10.1016/j.cellsig.2012.11.008 ER - TY - CHAP AU - Sotoca, A.M. AU - Weber, M. AU - Zoelen, E.J.J. van PY - 2013 UR - https://hdl.handle.net/2066/103443 PB - Hershey : IGI Global TI - Gene Expression Regulation underlying Osteo-, Adipo-, and Chondro-Genic Lineage Commitment of Human Mesenchymal Stem Cells EP - 94 SN - 9781466625068 SP - 76 CT - Daskalaki, A. (ed.), Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications DO - https://doi.org/10.4018/978-1-4666-2506-8.ch004 ER - TY - JOUR AU - Meijer, I.M. AU - Kerperien, J. AU - Sotoca, A.M. AU - Zoelen, Joop van AU - Leeuwen, J.E. van PY - 2013 UR - https://hdl.handle.net/2066/111442 TI - The Usp8 deubiquitination enzyme is post-translationally modified by tyrosine and serine phosphorylation EP - 930 SN - 0898-6568 IS - iss. 4 SP - 919 JF - Cellular Signalling VL - vol. 25 DO - https://doi.org/10.1016/j.cellsig.2013.01.003 ER - TY - JOUR AU - Hupkes, M. AU - Azevedo, R. AU - Jansen, H. AU - Zoelen, Joop van AU - Dechering, K.J. PY - 2013 UR - https://hdl.handle.net/2066/111520 TI - Identification of Novel Bacterial M.SssI DNA Methyltransferase Inhibitors EP - 355 SN - 1087-0571 IS - iss. 3 SP - 348 JF - Journal of Biomolecular Screening VL - vol. 18 DO - https://doi.org/10.1177/1087057112465009 ER - TY - JOUR AU - Sotoca, A.M. AU - Roelofs-Hendriks, J. AU - Boeren, S. AU - Kraan, P.M. van der AU - Vervoort, J. AU - Zoelen, E.J.J. van AU - Piek, E. PY - 2013 UR - https://hdl.handle.net/2066/122879 TI - Comparative proteome approach demonstrates that platelet-derived growth factor C and D efficiently induce proliferation while maintaining multipotency of hMSCs EP - 2662 SN - 0014-4827 IS - iss. 17 SP - 2649 JF - Experimental Cell Research VL - vol. 319 DO - https://doi.org/10.1016/j.yexcr.2013.07.027 ER - TY - JOUR AU - Weber, M. AU - Henkel, S.G. AU - Vlaic, S. AU - Guthke, R. AU - Zoelen, Joop van AU - Driesch, D. PY - 2013 UR - https://hdl.handle.net/2066/227601 TI - Inference of dynamical gene-regulatory networks based on time-resolved multi-stimuli multi-experiment data applying NetGenerator V2.0 EP - 1(28) SN - 1752-0509 IS - iss. 1 SP - 1(1) JF - BMC Systems Biology VL - vol. 7 DO - https://doi.org/10.1186/1752-0509-7-1 ER - TY - JOUR AU - Weber, M. AU - Henkel, S. AU - Vlaic, S. AU - Guthke, R. AU - Zoelen, Joop van AU - Driesch, D. PY - 2013 UR - https://hdl.handle.net/2066/111523 TI - Inference of dynamical gene-regulatory networks based on time-resolved multi-stimuli multi-experiment data applying NetGenerator V2.0 EP - 16 SN - 1752-0509 IS - iss. 1 SP - 1 JF - BMC Systems Biology VL - vol. 7 DO - https://doi.org/10.1186/1752-0509-7-1 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/111523/111523.pdf?sequence=1 ER - TY - JOUR AU - Weber, M. AU - Sotoca, A.M. AU - Kupfer, P. AU - Guthke, R. AU - Zoelen, E.J.J. van PY - 2013 UR - https://hdl.handle.net/2066/122885 AB - B TI - Dynamic modelling of microrna regulation during mesenchymal stem cell differentiation SN - 1752-0509 IS - iss. 1 SP - 124 JF - BMC Systems Biology VL - vol. 7 DO - https://doi.org/10.1186/1752-0509-7-124 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/122885/122885.pdf?sequence=1 ER - TY - JOUR AU - Hensen, S.M.M. AU - Heldens, L. AU - Enckevort, C.M.W. van AU - Genesen, S.T. van AU - Pruijn, G.J.M. AU - Lubsen, N.H. PY - 2012 UR - https://hdl.handle.net/2066/103412 TI - Heat shock factor 1 is inactivated by amino acid deprivation EP - 755 SN - 1355-8145 IS - iss. 6 SP - 743 JF - Cell Stress & Chaperones VL - vol. 17 DO - http://dx.doi.org/10.1007/s12192-012-0347-1 ER - TY - JOUR AU - Watering, F.C.J. van de AU - Beucken, J.J.J.P van den AU - Woning, S.P. van der AU - Briest, A. AU - Eek, A. AU - Qureshi, H. AU - Winnubst, L. AU - Boerman, O.C. AU - Jansen, J.B.M.J. PY - 2012 UR - https://hdl.handle.net/2066/108847 AB - Bone morphogenic protein-2 (BMP-2) is a well-known growth factor that can improve the biological performance of bone substitute materials. BMP-2 produced via bacterial expression systems are non-glycosylated (ng) whereas native and recombinant equivalents produced in mammalian cell expression systems are glycosylated (g) proteins. ngBMP-2 is less soluble, resulting in lower BMP-2 release from carriers as used as bone substitute materials. This seems promising for reducing the amount of included growth factor in bone substitute materials. Hence, it was hypothesized that ngBMP-2 would induce formation of the same amount of bone at an ectopic site at lower dosage as gBMP-2. To that end, gBMP-2 and ngBMP-2 were firstly in vitro comparatively evaluated for biological activity and release from a calcium phosphate (CaP) based bone substitute material. Thereafter, an ectopic implantation model in rats was used, in which gBMP-2 and ngBMP2 were loaded in various dosages (2-20 mug/implant) on the CaP-based bone substitute material and implanted for 4 and 12 weeks. The results revealed that both the in vitro biological activity of and the in vitro release of ngBMP-2 are lower compared to gBMP2. Upon ectopic implantation, however, ngBMP-2 loaded implants induced more bone formation at lower concentrations from 4-weeks onward compared to gBMP-2 equivalents, indicating the value of ngBMP-2 as a potential alternative for mammalian produced recombinant BMP-2 for bone regenerative therapies. TI - Non-glycosylated BMP-2 can induce ectopic bone formation at lower concentrations compared to glycosylated BMP-2. EP - 77 SN - 0168-3659 IS - iss. 1 SP - 69 JF - Journal of Controlled Release VL - vol. 159 DO - https://doi.org/10.1016/j.jconrel.2011.12.041 ER - TY - CHAP AU - Zoelen, E.J.J. van PY - 2012 UR - https://hdl.handle.net/2066/103404 PB - Brasov : Transilvania University Press TI - Growth control in normal cells, tumor cells and stem cells EP - 22 SP - 15 CT - Chesca, A. (ed.), Growth control in normal cells, tumor cells and stem cells ER - TY - JOUR AU - Meijer, I.M.J. AU - Rotterdam, W. van AU - Zoelen, Joop van AU - Leeuwen, J.E. van PY - 2012 UR - https://hdl.handle.net/2066/103684 TI - Recycling of EGFR and ErbB2 is associated with impaired Hrs tyrosine phosphorylation and decreased deubiquitination by AMSH EP - 1988 SN - 0898-6568 SP - 1981 JF - Cellular Signalling VL - vol. 24 DO - https://doi.org/10.1016/j.cellsig.2012.07.006 ER - TY - JOUR AU - Heldens, G. AU - Davidson, E.N AU - Vitters, E.L AU - Schreurs, B.W AU - Piek, E. AU - Berg, W.B. van den AU - Kraan, P.M. van der PY - 2012 UR - https://hdl.handle.net/2066/93779 TI - Catabolic Factors and Osteoarthritis-Conditioned Medium Inhibit Chondrogenesis of Human Mesenchymal Stem Cells EP - 54 SN - 1937-3341 IS - iss. 1-2 SP - 45 JF - Tissue Engineering Part A VL - vol. 18 DO - https://doi.org/10.1089/ten.tea.2011.0083 ER - TY - THES AU - Dernison, M.M. PY - 2012 SN - 9789461914125 UR - https://hdl.handle.net/2066/100592 PB - [S.l. : s.n.] TI - Pacemaking activity in normal rat kidney fibroblasts N1 - Radboud Universiteit Nijmegen, 13 november 2012 N1 - Promotores : Zoelen, E.J.J. van, Gielen, C.C.A.M. Co-promotor : Theuvenet, A.P.R. PS - 190 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/100592/100592.pdf?sequence=1 ER - TY - JOUR AU - Almirza, W.H.M.A. AU - Peters, P.H.J. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2012 UR - https://hdl.handle.net/2066/94044 TI - Role of Trpc channels, Stim1 and Orai1 in PGF(2 alpha)-induced calcium signaling in NRK fibroblasts EP - 21 SN - 0143-4160 IS - iss. 1 SP - 12 JF - Cell Calcium VL - vol. 51 DO - https://doi.org/10.1016/j.ceca.2011.10.001 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/94044/94044.pdf?sequence=1 ER - TY - JOUR AU - Jenks, B.G. AU - Galas, L. AU - Kuribara, M. AU - Desrues, L. AU - Kidane, A.H. AU - Vaudry, H. AU - Scheenen, W.J.J.M. AU - Roubos, E.W. AU - Tonon, M.C. PY - 2011 UR - https://hdl.handle.net/2066/84446 TI - Analysis of the melanotrope cell neuroendocrine interface in two amphibian species, Rana ridibunda and Xenopus laevis: A celebration of 35 years of collaborative research EP - 67 SN - 0016-6480 IS - iss. 1 SP - 57 JF - General and Comparative Endocrinology VL - vol. 170 PS - 11 p. DO - https://doi.org/10.1016/j.ygcen.2010.09.022 ER - TY - JOUR AU - Hupkes, M. AU - Someren, E.P. van AU - Middelkamp, S.H.A. AU - Piek, E. AU - Zoelen, Joop van AU - Dechering, K.J. PY - 2011 UR - https://hdl.handle.net/2066/91686 TI - DNA methylation restricts spontaneous multi-lineage differentiation of mesenchymal progenitor cells, but is stable during growth factor-induced terminal differentiation EP - 849 SN - 0167-4889 IS - iss. 5 SP - 839 JF - Biochimica et Biophysica Acta. Molecular Cell Research VL - vol. 1813 PS - 11 p. DO - https://doi.org/10.1016/j.bbamcr.2011.01.022 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/91686/91686.pdf?sequence=1 ER - TY - JOUR AU - Hupkes, M. AU - Jonsson, M.K. AU - Scheenen, W.J.J.M. AU - Rotterdam, W. van AU - Sotoca Covaleda, A.M. AU - Someren, E.P. van AU - Heyden, M.A. van der AU - Veen, T.A. van AU - Ravestein-Van Os, R.I. van AU - Bauerschmidt, S. AU - Piek, E. AU - Ypey, D.L. AU - Zoelen, E.J.J. van AU - Dechering, K.J. PY - 2011 UR - https://hdl.handle.net/2066/91778 TI - Epigenetics: DNA demethylation promotes skeletal myotube maturation EP - 3872 SN - 0892-6638 IS - iss. 11 SP - 3861 JF - The Faseb Journal VL - vol. 25 DO - https://doi.org/10.1096/fj.11-186122 ER - TY - JOUR AU - Kuribara, M. AU - Kidane, A.H. AU - Vos, G.A. AU - Gouw, D. de AU - Roubos, E.W. AU - Scheenen, W.J.J.M. AU - Jenks, B.G. PY - 2011 UR - https://hdl.handle.net/2066/91803 TI - Extracellular-signal regulated kinase regulates production of pro-opiomelanocortin in pituitary melanotroph cells. EP - 268 SN - 0953-8194 IS - iss. 3 SP - 261 JF - Journal of Neuroendocrinology VL - vol. 23 N1 - 1 maart 2011 DO - https://doi.org/10.1111/j.1365-2826.2010.02103.x ER - TY - JOUR AU - Vrolix, K. AU - Niks, E.H. AU - Panse, R. Le AU - Ostaijen-ten Dam, M.M. van AU - Muris, A.H. AU - Zijde, C.M. Jol-van der AU - Tol, M.J.D. van AU - Losen, M. AU - Molenaar, P.C. AU - Zoelen, E.J.J. van AU - Berrih-Aknin, S. AU - Baets, M.H. De AU - Verschuuren, J. AU - Martinez-Martinez, P. PY - 2011 UR - https://hdl.handle.net/2066/92264 TI - Reduced thymic expression of ErbB receptors without auto-antibodies against synaptic ErbB in myasthenia gravis EP - 165 SN - 0165-5728 IS - iss. 1-2 SP - 158 JF - Journal of Neuroimmunology VL - vol. 232 DO - https://doi.org/10.1016/j.jneuroim.2010.10.024 ER - TY - THES AU - Damen, E. PY - 2010 SN - 9789090254098 UR - https://hdl.handle.net/2066/77567 PB - [S.l. : s.n.] TI - The role of the retromer complex in receptor trafficking N1 - RU Radboud Universiteit Nijmegen, 5 juli 2010 PS - 245 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/77567/77567.pdf?sequence=1 ER - TY - THES AU - Woning, S.P. van der PY - 2010 SN - 9789090249636 UR - https://hdl.handle.net/2066/74880 PB - [S.l. : s.n.] TI - Molecular mechanisms that underlie the receptor specificity of ErbB ligands N1 - RU Radboud Universiteit Nijmegen, 12 januari 2010 PS - 243 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/74880/74880.pdf?sequence=1 ER - TY - JOUR AU - Kappe, G. AU - Purkiss, A.G. AU - Genesen, S.T. van AU - Slingsby, C AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83594 TI - Explosive expansion of beta gamma-crystallin genes in the ancestral vertebrate (vol 71, 219, 2010) EP - 318 SN - 0022-2844 IS - iss. 4 SP - 317 JF - Journal of Molecular Evolution VL - vol. 71 PS - 2 p. DO - http://dx.doi.org/10.1007/s00239-010-9387-2 ER - TY - JOUR AU - Kappe, G. AU - Purkiss, A.G. AU - Genesen, S.T. van AU - Slingsby, C AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83598 TI - Explosive expansion of beta gamma-crystallin genes in the ancestral vertebrate EP - 230 SN - 0022-2844 IS - iss. 3 SP - 219 JF - Journal of Molecular Evolution VL - vol. 71 PS - 12 p. DO - http://dx.doi.org/10.1007/s00239-010-9379-2 ER - TY - JOUR AU - Hageman, J. AU - Rujano, M.A. AU - Waarde, M. van AU - Kakkar, V. AU - Dirks, R.P. AU - Govorukhina, N. AU - Oosterveld-Hut, H.M.J. AU - Lubsen, N.H. AU - Kampinga, H.H. PY - 2010 UR - https://hdl.handle.net/2066/84379 TI - A dnajb chaperone subfamily with hdac-dependent activities suppresses toxic protein aggregation EP - 369 SN - 1097-2765 IS - iss. 3 SP - 355 JF - Molecular Cell VL - vol. 37 PS - 15 p. DO - http://dx.doi.org/10.1016/j.molcel.2010.01.001 ER - TY - JOUR AU - Haan, J.R. de AU - Wehrens, H.R.M.J. AU - Bauerschmidt, S. AU - Schaik, R.C. Van AU - Piek, E. AU - Buydens, L.M.C. PY - 2010 UR - https://hdl.handle.net/2066/83312 TI - Beyond single p-value cut-offs: Methods to improve decision making in GO enrichment analysis of microarray experiments EP - 18 SN - 1443-2250 IS - iss. 1 SP - 1 JF - Online Journal of Bioinformatics VL - vol. 11 PS - 18 p. ER - TY - JOUR AU - Dirks, R.P.H. AU - Geel, R. van AU - Hensen, S.M.M. AU - Genesen, S.T. van AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83587 TI - Manipulating heat shock factor-1 in xenopus tadpoles: Neuronal tissues are refractory to exogenous expression SN - 1932-6203 IS - iss. 4 JF - PLoS One VL - vol. 5 PS - 10 p. DO - https://doi.org/10.1371/journal.pone.0010158 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/83587/83587.pdf?sequence=1 ER - TY - JOUR AU - Dimke, H. AU - Wijst, J.A.J. van der AU - Alexander, T. AU - Meijer, I.M.J. AU - Mulder, G.M. AU - Goor, H. van AU - Tejpar, S. AU - Hoenderop, J.G.J. AU - Bindels, R.J.M. PY - 2010 UR - https://hdl.handle.net/2066/88763 AB - A mutation in pro-EGF causes isolated hypomagnesemia, and monoclonal antibodies targeting the extracellular domain of the EGF receptor (EGFR) affect epithelial Mg(2+) transport. The effect of the EGFR tyrosine kinase inhibitor erlotinib on Mg(2+) homeostasis, however, remains unknown. Here, we injected C57BL/6 mice with erlotinib for 23 days and observed a small but significant decrease in serum Mg(2+) concentrations at days 16 and 23, but the fractional excretion of Mg(2+) remained unchanged after 23 days. Semiquantitative immunohistochemical evaluation did not reveal detectable changes in renal expression of transient receptor potential melastatin 6 (TRPM6) protein, the channel that mediates Mg(2+) reabsorption. Patch clamp analysis in TRPM6-expressing cells demonstrated that 30 muM erlotinib inhibited EGF-induced changes in TRPM6 current density and tyrosine phosphorylation of EGFR; 0.3 muM erlotinib did not have these effects. Furthermore, 30 muM erlotinib inhibited EGF-stimulated increases in the mobile fraction of endomembrane TRPM6 channels. In summary, erlotinib can influence Mg(2+) handling but its effect on the systemic Mg(2+) concentration seems less potent than that observed with antibody-based EGFR inhibitors. These data suggest that typical human dosages of erlotinib are unlikely to severely affect serum Mg(2+) concentrations. TI - Effects of the EGFR Inhibitor Erlotinib on Magnesium Handling. EP - 1316 SN - 1046-6673 IS - iss. 8 SP - 1309 JF - Journal of the American Society of Nephrology VL - vol. 21 N1 - 1 augustus 2010 DO - https://doi.org/10.1681/ASN.2009111153 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/88763/88763.pdf?sequence=1 ER - TY - JOUR AU - Heldens, L. AU - Dirks, R.P.H. AU - Hensen, S.M.M. AU - Onnekink, C. AU - Genesen, S.T. van AU - Rustenburg, F. AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83516 TI - Co-chaperones are limiting in a depleted chaperone network EP - 4048 SN - 1420-682X IS - iss. 23 SP - 4035 JF - Cellular and Molecular Life Sciences VL - vol. 67 DO - https://doi.org/10.1007/s00018-010-0430-7 ER - TY - JOUR AU - Piek, E. AU - Sleumer, L.S. AU - Someren, E.P. van AU - Heuver, L. AU - Haan, J.R. de AU - Grijs, I. de AU - Gilissen, C.F.H.A. AU - Hendriks, J.M. AU - van Ravestein-van Os, R.I. AU - Bauerschmidt, S. AU - Dechering, K.J. AU - Zoelen, E.J.J. van PY - 2010 UR - https://hdl.handle.net/2066/84264 TI - Osteo-transcriptomics of human mesenchymal stem cells: Accelerated gene expression and osteoblast differentiation induced by vitamin d reveals c-myc as an enhancer of bmp2-induced osteogenesis EP - 627 SN - 8756-3282 IS - iss. 3 SP - 613 JF - Bone VL - vol. 46 PS - 15 p. DO - https://doi.org/10.1016/j.bone.2009.10.024 ER - TY - JOUR AU - Haan, J.R. de AU - Piek, E. AU - Schaik, R.C. Van AU - Vlieg, J. de AU - Bauerschmidt, S. AU - Buydens, L.M.C. AU - Wehrens, H.R.M.J. PY - 2010 UR - https://hdl.handle.net/2066/83407 TI - Integrating gene expression and go classification for pca by preclustering EP - 10 SN - 1471-2105 SP - 1 JF - BMC Bioinformatics VL - vol. 11 PS - 10 p. DO - https://doi.org/10.1186/1471-2105-11-158 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/83407/83407.pdf?sequence=1 ER - TY - JOUR AU - Vaes, B.L.T. AU - Lute, C. AU - Woning, S.P. van der AU - Piek, E. AU - Vermeer, J. AU - Blom, H.J. AU - Mathers, J.C. AU - Muller, M. AU - Groot, L. de AU - Steegenga, W.T. PY - 2010 UR - https://hdl.handle.net/2066/83427 TI - Inhibition of methylation decreases osteoblast differentiation via a non-DNA-dependent methylation mechanism EP - 523 SN - 8756-3282 IS - iss. 2 SP - 514 JF - Bone VL - vol. 46 PS - 10 p. DO - https://doi.org/10.1016/j.bone.2009.09.033 ER - TY - JOUR AU - Meijer, I.M.J. AU - Leeuwen, J.E.M. van PY - 2010 UR - https://hdl.handle.net/2066/83789 TI - Erbb2 is a target for usp8-deiated deubquitination EP - 467 SN - 0898-6568 IS - iss. 2 SP - 458 JF - Cellular Signalling VL - vol. 23 PS - 10 p. DO - https://doi.org/10.1016/j.cellsig.2010.10.023 ER - TY - JOUR AU - Almirza, W.H.M. AU - Peters, P.H.J. AU - Meerwijk, W.P.M. van AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2010 UR - https://hdl.handle.net/2066/83828 TI - Different roles of inositol 1,4,5-trisphosphate receptor subtypes in prostaglandin f-2 alpha-induced calcium oscillations and pacemaking activity of nrk fibroblasts EP - 553 SN - 0143-4160 IS - iss. 6 SP - 544 JF - Cell Calcium VL - vol. 47 DO - https://doi.org/10.1016/j.ceca.2010.05.004 ER - TY - JOUR AU - Dimke, H. AU - Wijst, J.A.J. van der AU - Alexander, T. AU - Meijer, I.M.J. AU - Mulder, G.M. AU - Goor, H. van AU - Tejpar, S. AU - Hoenderop, J.G.J. AU - Bindels, R.J. PY - 2010 UR - https://hdl.handle.net/2066/84268 TI - Effects of the egfr inhibitor erlotinib on magnesium handling EP - 1316 SN - 1046-6673 IS - iss. 8 SP - 1309 JF - Journal of the American Society of Nephrology VL - vol. 21 PS - 18 p. DO - https://doi.org/10.1681/ASN.2009111153 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/84268/84268.pdf?sequence=1 ER - TY - JOUR AU - Dernison, M.M. AU - Almirza, W.H.M.A. AU - Kusters, J.M.A.M. AU - Meerwijk, W.P.M. van AU - Gielen, C.C.A.M. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2010 UR - https://hdl.handle.net/2066/84300 TI - Growth-dependent modulation of capacitative calcium entry in normal rat kidney fibroblasts EP - 1053 SN - 0898-6568 IS - iss. 7 SP - 1044 JF - Cellular Signalling VL - vol. 22 PS - 10 p. DO - https://doi.org/10.1016/j.cellsig.2010.02.007 ER - TY - JOUR AU - Sundvall, M. AU - Veikkolainen, V. AU - Kurppa, K. AU - Salah, Z. AU - Tvorogov, D. AU - Zoelen, Joop van AU - Aqeilan, R. AU - Elenius, K. PY - 2010 UR - https://hdl.handle.net/2066/83476 TI - Cell death or survival promoted by alternative isoforms of erbb4 EP - 4286 SN - 1059-1524 IS - iss. 23 SP - 4275 JF - Molecular Biology of the Cell VL - vol. 21 PS - 12 p. DO - https://doi.org/10.1091/mbc.E10-04-0332 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/83476/83476.pdf?sequence=1 ER - TY - JOUR AU - Haan, J.R. de AU - Bauerschmidt, S. AU - Schaik, R.C. Van AU - Piek, E. AU - Buydens, L.M.C. AU - Wehrens, H.R.M.J. PY - 2009 UR - https://hdl.handle.net/2066/75948 TI - Robust ANOVA for microarray data EP - 44 SN - 0169-7439 IS - iss. 1 SP - 38 JF - Chemometrics and Intelligent Laboratory Systems VL - vol. 98 PS - 7 p. DO - https://doi.org/10.1016/j.chemolab.2009.04.011 ER - TY - JOUR AU - Hannink, G.J. AU - Piek, E. AU - Hendriks, J.M. AU - Kraan, P.M. van der AU - Schreurs, B.W. AU - Buma, P. PY - 2009 UR - https://hdl.handle.net/2066/75115 TI - Biological effects of rinsing morsellised bone grafts before and after impaction. EP - 866 SN - 0341-2695 IS - iss. 3 SP - 861 JF - International Orthopaedics VL - vol. 33 PS - 6 p. DO - https://doi.org/10.1007/s00264-007-0513-8 ER - TY - JOUR AU - Woning, S.P. van der AU - Venselaar, H. AU - Rotterdam, W. van AU - Jacobs-Oomen, S. AU - Leeuwen, J.E.M. van AU - Zoelen, E.J.J. van PY - 2009 UR - https://hdl.handle.net/2066/75949 TI - Role of the C-terminal linear region of EGF-like growth factors in ErbB specificity EP - 172 SN - 0897-7194 IS - iss. 3 SP - 163 JF - Growth Factors VL - vol. 27 PS - 10 p. DO - https://doi.org/10.1080/08977190902891010 ER - TY - JOUR AU - Woning, S.P. van der AU - Venselaar, H. AU - Rotterdam, W. van AU - Jacobs-Oomen, S. AU - Leeuwen, J.E.M. van AU - Zoelen, E.J.J. van PY - 2009 UR - https://hdl.handle.net/2066/75950 AB - The epidermal growth factor (EGF)-like growth factors bind their ErbB receptors in a highly selective manner. Recently, we have shown that the sequence YYDLL in the C-terminal linear region is compatible with binding to all ligand-binding ErbB receptors. In the present study, we show that introduction of the YYDLL sequence into the ErbB1 specific ligands EGF and transforming growth factor-alpha (TGFalpha) broadened their receptor specificity towards ErbB4. Upon introduction of the YYDLL sequence into epiregulin, which by itself binds ErbB1 and ErbB4 but not ErbB3, its binding specificity was broadened to ErbB3, concomitant with enhanced affinity for ErbB4. Introduction of the YYDLL sequence into NRG1beta resulted in a 10-fold increase in affinity for ErbB3, without affecting its receptor specificity. Remarkably, the strongly enhanced affinity for ErbB3 negatively influenced their mitogenic activity towards cells coexpressing ErbB2 and ErbB3. These observations are discussed in terms of the optimised ErbB affinity, selectivity and mitogenic potential that have taken place during evolution. TI - Role of the C-terminal linear region of EGF-like growth factors in ErbB specificity. EP - 172 SN - 0897-7194 IS - iss. 3 SP - 163 JF - Growth Factors VL - vol. 27 DO - https://doi.org/10.1080/08977190902891010 ER - TY - JOUR AU - Jansen, S.M. AU - Sleumer, L.S. AU - Damen, E. AU - Meijer, I.M.J. AU - Zoelen, E.J.J. van AU - Leeuwen, J.E.M. van PY - 2009 UR - https://hdl.handle.net/2066/75956 TI - ErbB2 and ErbB4 Cbl binding sites can functionally replace the ErbB1 Cbl binding site EP - 818 SN - 0898-6568 IS - iss. 5 SP - 810 JF - Cellular Signalling VL - vol. 21 PS - 9 p. DO - https://doi.org/10.1016/j.cellsig.2009.01.028 ER - TY - JOUR AU - Woning, S.P. van der AU - Zoelen, E.J.J. van PY - 2009 UR - https://hdl.handle.net/2066/75966 TI - Quantification of ErbB3 receptor density on human breast cancer cells, using a stable radio-labeled mutant of NRG1beta EP - 289 SN - 0006-291X IS - iss. 2 SP - 285 JF - Biochemical and Biophysical Research Communications VL - vol. 378 PS - 5 p. DO - https://doi.org/10.1016/j.bbrc.2008.11.034 ER - TY - JOUR AU - Toepoel, M. AU - Steegers-Theunissen, R.P.M. AU - Ouborg, N.J. AU - Franke, B. AU - et al. AU - Joosten, P.H.L.J. AU - Zoelen, E.J.J. van PY - 2009 UR - https://hdl.handle.net/2066/76108 TI - Interaction of PDGFRA Promoter Haplotypes and Maternal Environmental Exposures in the Risk of Spina Bifida EP - 636 SN - 1542-0752 IS - iss. 7 SP - 629 JF - Birth Defects Research Part A-Clinical and Molecular Teratology VL - vol. 85 PS - 8 p. DO - https://doi.org/10.1002/bdra.20574 ER - TY - JOUR AU - Toepoel, M. AU - Steegers-Theunissen, R.P.M. AU - Ouborg, N.J. AU - Franke, B. AU - Gonzalez-Zuloet Ladd, A.M. AU - Joosten, P.H. AU - Zoelen, E.J.J. van PY - 2009 UR - https://hdl.handle.net/2066/80133 AB - BACKGROUND: Neural tube defects are multifactorial malformations involving both environmental exposures, such as maternal nutrition, and genetic factors. Aberrant expression of the platelet-derived growth factor alpha-receptor (PDGFRA) gene has been implicated in neural-tube-defect etiology in both mice and humans. METHODS: We investigated possible interactions between the PDGFRA promoter haplotype of mother and child, as well as maternal glucose, myo-inositol, and zinc levels, in relation to spina bifida offspring. Distributions were determined of the PDGFRA promoter haplotypes H1 and H2 in a Dutch cohort, consisting of 88 spina bifida children with 56 of their mothers, and 74 control children with 72 of their mothers, as well as maternal plasma glucose, myo-inositol, and red blood cell zinc concentrations. RESULTS: A significantly higher frequency of H1 was observed in children with spina bifida than in controls (30.1 vs. 20.3%; OR = 1.69, 95% CI 1.02-2.83). High maternal body mass index (BMI) and glucose were significant risk factors for both H1 and H2 children, whereas low myo-inositol and zinc were risk factors for H2 but not for H1 children. Stepwise multiple logistic regression analysis showed that high maternal glucose and low myo-inositol are the main risk factors for H2 spina bifida children, whereas for H1 spina bifida children, maternal BMI was the main risk factor. Interestingly, H1 mothers (median 165.5 cm) showed a significantly lower body height than H2 mothers (median 169.1 cm; p = 0.003). CONCLUSIONS: These data suggest that the child's PDGFRA promoter haplotype is differentially sensitive for periconceptional exposure to glucose, myo-inositol, and zinc in the risk of spina bifida. TI - Interaction of PDGFRA promoter haplotypes and maternal environmental exposures in the risk of spina bifida. EP - 636 SN - 1542-0752 IS - iss. 7 SP - 629 JF - Birth Defects Research Part A-Clinical and Molecular Teratology VL - vol. 85 DO - https://doi.org/10.1002/bdra.20574 ER - TY - JOUR AU - Kidane, A.H. AU - Heinrich, G. AU - Dirks, R.P.H. AU - Ruyck, B.A. de AU - Lubsen, N.H. AU - Roubos, E.W. AU - Jenks, B.G. PY - 2008 UR - https://hdl.handle.net/2066/72169 TI - Differential neuroendocrine expression of multiple brain-derived neurotrophic factor transcripts EP - 1368 SN - 0013-7227 IS - iss. 3 SP - 1361 JF - Endocrinology VL - vol. 150 PS - 8 p. DO - http://dx.doi.org/10.1210/en.2008-0993 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/72169/72169.pdf?sequence=1 ER - TY - JOUR AU - Franck, E. AU - Hulsen, T. AU - Huynen, M.A. AU - Jong, W.W.W. de AU - Lubsen, N.H. AU - Madsen, O. PY - 2008 UR - https://hdl.handle.net/2066/70352 AB - The orientation of closely linked genes in mammalian genomes is not random: there are more head-to-head (h2h) gene pairs than expected. To understand the origin of this enrichment in h2h gene pairs, we have analyzed the phylogenetic distribution of gene pairs separated by less than 600 bp of intergenic DNA (gene duos). We show here that a lack of head-to-tail (h2t) gene duos is an even more distinctive characteristic of mammalian genomes, with the platypus genome as the only exception. In nonmammalian vertebrate and in nonvertebrate genomes, the frequency of h2h, h2t, and tail-to-tail (t2t) gene duos is close to random. In tetrapod genomes, the h2t and t2t gene duos are more likely to be part of a larger gene cluster of closely spaced genes than h2h gene duos; in fish and urochordate genomes, the reverse is seen. In human and mouse tissues, the expression profiles of gene duos were skewed toward positive coexpression, irrespective of orientation. The organization of orthologs of both members of about 40% of the human gene duos could be traced in other species, enabling a prediction of the organization at the branch points of gnathostomes, tetrapods, amniotes, and euarchontoglires. The accumulation of h2h gene duos started in tetrapods, whereas that of h2t and t2t gene duos only started in amniotes. The apparent lack of evolutionary conservation of h2t and t2t gene duos relative to that of h2h gene duos is thus a result of their relatively late origin in the lineage leading to mammals; we show that once they are formed h2t and t2t gene duos are as stable as h2h gene duos. TI - Evolution of closely linked gene pairs in vertebrate genomes. EP - 1921 SN - 0737-4038 IS - iss. 9 SP - 1909 JF - Molecular Biology and Evolution VL - vol. 25 PS - 12 p. DO - http://dx.doi.org/10.1093/molbev/msn136 ER - TY - JOUR AU - Franck, E. AU - Hulsen, T. AU - Huynen, M.A. AU - Jong, W.W.W. de AU - Lubsen, N.H. AU - Madsen, O. PY - 2008 UR - https://hdl.handle.net/2066/70351 TI - Evolution of closely linked gene pairs in vertebrate genomes EP - 1921 SN - 0737-4038 IS - iss. 9 SP - 1909 JF - Molecular Biology and Evolution VL - vol. 25 PS - 13 p. DO - https://doi.org/10.1093/molbev/msn136 ER - TY - JOUR AU - Hannink, G.J. AU - Piek, E. AU - Hendriks, J.M.A. AU - Kraan, P.M. van der AU - Schreurs, W AU - Buma, P. PY - 2008 UR - https://hdl.handle.net/2066/70162 TI - Biological effects of rinsing morsellised bone grafts before and after impaction EP - 6 SN - 0341-2695 IS - iss. 1 SP - 1 JF - International Orthopaedics VL - vol. e-pub PS - 6 p. DO - https://doi.org/10.1007/s00264-007-0513-8 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/70162/70162.pdf?sequence=1 ER - TY - JOUR AU - Christis, C. AU - Lubsen, N.H. AU - Braakman, I. PY - 2008 UR - https://hdl.handle.net/2066/71339 TI - Protein folding includes oligomerization - examples from the endoplasmic reticulum and cytosol EP - 4727 SN - 1742-464X IS - iss. 19 SP - 4700 JF - FEBS Journal VL - vol. 275 DO - https://doi.org/10.1111/j.1742-4658.2008.06590.x ER - TY - JOUR AU - Dernison, M.M. AU - Kusters, J.M.A.M. AU - Peters, P.H.J. AU - Meerwijk, W.P. van AU - Ypey, D.L. AU - Gielen, C.C.A.M. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2008 UR - https://hdl.handle.net/2066/69487 AB - Cultures of normal rat kidney (NRK) fibroblasts may display spontaneous calcium action potentials which propagate throughout the cellular monolayer. Pacemaking activity of NRK cells was studied by patch clamp electrophysiology and vital calcium imaging, using a new experimental approach in which a ring was placed on the monolayer in order to physically separate pacemakers within or under the ring and follower cells outside the ring. Stimulation of cells inside the ring with IP(3)-generating hormones such as prostaglandin F(2alpha) (PGF(2alpha)) resulted in the induction of periodic action potentials outside the ring, which were abolished when the L-type calcium channel blocker nifedipine was added outside the ring, but not inside the ring. PGF(2alpha)-treated cells displayed asynchronous IP(3)-mediated calcium oscillations of variable frequency, while follower cells outside the ring showed synchronous calcium transients which coincided with the propagating action potential. Mathematical modelling indicated that addition of PGF(2alpha) inside the ring induced both a membrane potential gradient and an intracellular IP(3) gradient, both of which are essential for the induction of pacemaking activity under the ring. These data show that intercellular coupling between PGF(2alpha)-treated and non-treated cells is essential for the generation of a functional pacemaker area whereby synchronization of calcium oscillations occurs by activation of L-type calcium channels. TI - Local induction of pacemaking activity in a monolayer of electrically coupled quiescent NRK fibroblasts. EP - 440 SN - 0143-4160 IS - iss. 5 SP - 429 JF - Cell Calcium VL - vol. 44 PS - 12 p. DO - https://doi.org/10.1016/j.ceca.2008.02.005 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/69487/69487.pdf?sequence=1 ER - TY - JOUR AU - Jong, J. de AU - Stoop, H. AU - Gillis, A.J. AU - Hersmus, R. AU - Gurp, R.J. Van AU - Geijn, G.J.M. van de AU - Drunen, E. van AU - Beverloo, H.B. AU - Schneider, D.T. AU - Sherlock, J.K. AU - Baeten, J. AU - Kitazawa, S. AU - Zoelen, E.J.J. van AU - Roozendaal, K. van AU - Oosterhuis, J.W. AU - Looijenga, L.H.J. PY - 2008 UR - https://hdl.handle.net/2066/70202 TI - Further characterization of the first seminoma cell line TCam-2 EP - 196 SN - 1045-2257 IS - iss. 3 SP - 185 JF - Genes, Chromosomes & Cancer VL - vol. 47 PS - 12 p. DO - https://doi.org/10.1002/gcc.20520 ER - TY - JOUR AU - Toepoel, M. AU - Joosten, P. AU - Knobbe, C.B. AU - Afink, G.B. AU - Zotz, R.B. AU - Steegers-Theunissen, R.P.M. AU - Reifenberger, G. AU - Zoelen, E.J.J. van PY - 2008 UR - https://hdl.handle.net/2066/72306 TI - Haplotype-specific expression of the human PDGFRA gene correlates with the risk of glioblastomas EP - 329 SN - 0020-7136 IS - iss. 2 SP - 322 JF - International Journal of Cancer VL - vol. 123 PS - 8 p. DO - https://doi.org/10.1002/ijc.23432 ER - TY - JOUR AU - Almirza, W.H. AU - Dernison, M.M. AU - Peters, P.H.J. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2008 UR - https://hdl.handle.net/2066/72307 TI - Role of the prostanoid FP receptor in action potential generation and phenotypic transformation of NRK fibroblasts EP - 2029 SN - 0898-6568 IS - iss. 11 SP - 2022 JF - Cellular Signalling VL - vol. 20 PS - 8 p. DO - https://doi.org/10.1016/j.cellsig.2008.07.013 ER - TY - JOUR AU - Kusters, J.M.A.M. AU - Meerwijk, W.P. van AU - Ypey, D.L. AU - Theuvenet, A.P.R. AU - Gielen, C.C.A.M. PY - 2008 UR - https://hdl.handle.net/2066/70278 AB - We have investigated synchronization and propagation of calcium oscillations, mediated by gap junctional excitation transmission. For that purpose we used an experimentally based model of normal rat kidney (NRK) cells, electrically coupled in a one-dimensional configuration (linear strand). Fibroblasts such as NRK cells can form an excitable syncytium and generate spontaneous inositol 1,4,5-trisphosphate (IP(3))-mediated intracellular calcium waves, which may spread over a monolayer culture in a coordinated fashion. An intracellular calcium oscillation in a pacemaker cell causes a membrane depolarization from within that cell via calcium-activated chloride channels, leading to an L-type calcium channel-based action potential (AP) in that cell. This AP is then transmitted to the electrically connected neighbor cell, and the calcium inflow during that transmitted AP triggers a calcium wave in that neighbor cell by opening of IP(3) receptor channels, causing calcium-induced calcium release (CICR). In this way the calcium wave of the pacemaker cell is rapidly propagated by the electrically transmitted AP. Propagation of APs in a strand of cells depends on the number of terminal pacemaker cells, the L-type calcium conductance of the cells, and the electrical coupling between the cells. Our results show that the coupling between IP(3)-mediated calcium oscillations and AP firing provides a robust mechanism for fast propagation of activity across a network of cells, which is representative for many other cell types such as gastrointestinal cells, urethral cells, and pacemaker cells in the heart. TI - Fast calcium wave propagation mediated by electrically conducted excitation and boosted by CICR. EP - C930 SN - 0363-6143 IS - iss. 4 SP - C917 JF - American Journal of Physiology : Cell Physiology VL - vol. 294 PS - 14 p. DO - https://doi.org/10.1152/ajpcell.00181.2007 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/70278/70278.pdf?sequence=2 ER - TY - GEN AU - Lubsen, N.H. PY - 2008 UR - https://hdl.handle.net/2066/129288 TI - Met het oog op stress N1 - Rede bij Ambtsaanvaarding, 26 juni 2008 N1 - Radboud Universiteit Nijmegen : [S.n.] PS - 11 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/129288/129288.pdf?sequence=1 ER - TY - BOOK AU - Alliston, T. AU - Piek, E. AU - Derynck, R. PY - 2007 UR - https://hdl.handle.net/2066/36538 PB - New York, USA : Cold Spring Harbor Laboratory Press TI - TGF-Bèta family signaling in skeletal development, maintenance, and disease. ER - TY - BOOK AU - Derynck, R. AU - Piek, E. AU - Schneider, R.A. AU - Choy, L. AU - Alliston, T. PY - 2007 UR - https://hdl.handle.net/2066/36539 PB - [S.l.] : Cold Spring Harbor Laboratory Press TI - TGF-beta family signaling in mesenchymal differentiation ER - TY - JOUR AU - Haan, J.R. de AU - Wehrens, Ron AU - Bauerschmidt, S. AU - Piek, E. AU - Schaik, R.C. Van AU - Buydens, L.M.C. PY - 2007 UR - https://hdl.handle.net/2066/34606 TI - Interpretation of ANOVA models for microarray data using PCA. EP - 190 SN - 1367-4803 IS - iss. 2 SP - 184 JF - Bioinformatics VL - vol. 23 DO - http://dx.doi.org/10.1093/bioinformatics/btl572 ER - TY - JOUR AU - Alwan, H.A.J. AU - Leeuwen, J.E.M. van PY - 2007 UR - https://hdl.handle.net/2066/36647 AB - Whereas poly-ubiquitination targets protein substrates for proteasomal degradation, mono-ubiquitination is known to regulate protein trafficking in the endosomal system and to target cargo proteins for lysosomal degradation. The role of the de-ubiquitinating enzymes AMSH and UBPY in endosomal trafficking of cargo proteins such as the epidermal growth factor receptor (EGFR) has only very recently been the subject of study and is already a matter of debate. Although one report (Mizuno, E., Iura, T., Mukai, A., Yoshimori, T., Kitamura, N., and Komada, M. (2005) Mol. Biol. Cell 16, 5163-5174) concludes that UBPY negatively regulates EGFR degradation by de-ubiquitinating the EGFR on endosomes, another report (Row, P. E., Prior, I. A., McCullough, J., Clague, M. J., and Urbe, S. (2006) J. Biol. Chem. 281, 12618-12624) concludes that UBPY-mediated EGFR de-ubiquitination is essential for EGFR degradation. Here, we demonstrate that Usp8/UBPY, the mammalian ortholog of budding yeast Ubp4/Doa4, constitutively co-precipitates in a bivalent manner with the EGFR. Moreover, UBPY is a substrate for Src-family tyrosine kinases that are activated after ligand-induced EGFR activation. Using overexpression of three different recombinant dominant negative UBPY mutants (UBPY C748A mutant, UBPY 1-505, and UBPY 640-1080) in NIH3T3 and HEK293 cells, we demonstrate that UBPY affects both constitutive and ligand-induced (i) EGFR ubiquitination, (ii) EGFR expression levels, and (iii) the appearance of intermediate EGFR degradation products as well as (iv) downstream mitogen-activated protein kinase signal transduction. Our findings provide further evidence in favor of the model that UBPY-mediated EGFR de-ubiquitination promotes EGFR degradation. TI - UBPY-mediated epidermal growth factor receptor (EGFR) de-ubiquitination promotes EGFR degradation EP - 1669 SN - 1083-351X IS - iss. 3 SP - 1658 JF - Journal of Biological Chemistry VL - vol. 282 DO - https://doi.org/10.1074/jbc.M604711200 ER - TY - CONF AU - Kusters, J.M. AU - Cortes, J.M. AU - Meerwijk, W.P. van AU - Ypey, D.L. AU - Theuvenet, A.P.R. AU - Gielen, C.C.A.M. PY - 2007 UR - https://hdl.handle.net/2066/36278 PB - American Institute of Physics : American Institute of Physics TI - Oscillatory activity in cells: Multi-stability and hysteresis. In Cooperative behavior in neural systems EP - 50 SN - 0735403902 SP - 40 CT - AIP Conference Proceedings L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/36278/36278.pdf?sequence=1 ER - TY - JOUR AU - Kusters, J.M. AU - Cortes, J.M. AU - Meerwijk, W.P. van AU - Ypey, D.L. AU - Theuvenet, A.P.R. AU - Gielen, C.C.A.M. PY - 2007 UR - https://hdl.handle.net/2066/34645 AB - Many cells reveal oscillatory behavior. Some cells reveal action-potential firing resulting from Hodgkin-Huxley (HH) type dynamics of ion channels in the cell membrane. Another type of oscillation relates to periodic inositol triphospate (IP3)-mediated calcium transients in the cytosol. In this study we present a bifurcation analysis of a cell with an excitable membrane and an IP3-mediated intracellular calcium oscillator. With IP3 concentration as a control parameter the model reveals a complex, rich spectrum of both stable and unstable solutions with hysteresis corresponding to experimental data. Our results reveal the emergence of complex behavior due to interactions between subcomponents with a relatively simple dynamical behavior. TI - Hysteresis and bistability in a realistic cell model for calcium oscillations and action potential firing EP - 098107-4 SN - 0031-9007 IS - iss. 9 SP - 098107 JF - Physical Review Letters VL - vol. 98 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/34645/34645.pdf?sequence=1 ER - TY - JOUR AU - Kusters, J.M.A.M. AU - Cortes, J.M. AU - Meerwijk, W.P. van AU - Ypey, D.L. AU - Theuvenet, A.P.R. AU - Gielen, C.C.A.M. PY - 2007 UR - https://hdl.handle.net/2066/34646 AB - Many cells reveal oscillatory behavior. Some cells reveal action-potential firing resulting from Hodgkin-Huxley (HH) type dynamics of ion channels in the cell membrane. Another type of oscillation relates to periodic inositol triphospate (IP3)-mediated calcium transients in the cytosol. In this study we present a bifurcation analysis of a cell with an excitable membrane and an IP3-mediated intracellular calcium oscillator. With IP3 concentration as a control parameter the model reveals a complex, rich spectrum of both stable and unstable solutions with hysteresis corresponding to experimental data. Our results reveal the emergence of complex behavior due to interactions between subcomponents with a relatively simple dynamical behavior. TI - Hysteresis and bistability in a realistic cell model for calcium oscillations and action potential firing. EP - 098107 SN - 0031-9007 IS - iss. 9 SP - 098107 JF - Physical Review Letters VL - vol. 98 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/34646/34646.pdf?sequence=1 ER - TY - CHAP AU - Kusters, J.M.A.M. AU - Cortes, J.M. AU - Meerwijk, W.P.M. van AU - Ypey, D.L. AU - Theuvenet, A.P.R. AU - Gielen, C.C.A.M. PY - 2007 UR - https://hdl.handle.net/2066/129291 PB - Meville, New York : American Institute of Physics TI - Oscillatory activity in cells: Multi-stability and hysteresis. EP - 50 SP - 40 CT - Ninth Granada Lectures ER - TY - THES AU - Vaes, B.L.T. PY - 2007 SN - 9789090218533 UR - https://hdl.handle.net/2066/30023 AB - The frequently occurring bone disorder osteoporosis is characterized by a strong increase in bone fracture risk, caused by a dramatically disturbed balance in the activity of the cells that degrade bone (osteoclasts) and cells that synthesize new bone (osteoblasts). Therapies against osteoporosis are currently based on inhibition of osteoclast activity in order to reduce the amount of bone resorption. An alternative strategy would be to stimulate new bone formation by enhancing the activity of osteoblasts. The main objective of this thesis was to identify genes of which expression and function are specifically associated with the differentiation of mesenchymal stem cells into osteoblasts. Therefore, we used microarray-based gene expression profiling to study the transcriptome of differentiating osteoblasts during in vitro cell culture as well as in their physiological context during skeletogenesis. The analyses revealed many genes of which expression in osteoblasts had not previously been detected. Novel markers of osteoblast differentiation and bone development were identified as well as putative in vivo target genes of the transcription factor Runx2 which is a key regulator of skeletal development. One of the markers identified encodes the protein Wif1 (Wnt Inhibitory Factor 1), which was shown to be specifically expressed in osteoblasts during embryonic development. Typically, genes encoding antagonists of the Wnt signalling pathway were strongly expressed during late stages of osteoblast differentiation and implicate an important role of Wnt antagonism in the maturation of osteoblasts. Given the role of Wnt signalling in the maintenance of bone mass, this class of proteins may be potential drug targets to treat bone disorders. This thesis shows that the combination of microarray technology and bioinformatic analyses has provided new possibilities to the understanding of bone biology. Consequently, our studies have contributed to a more detailed knowledge on osteoblast differentiation and provide focus points to develop strategies that will prevent and treat bone disorders. PB - Nijmegen : [S.n.] TI - Exploration of the mouse osteoblast transcriptome N1 - Radboud University, Applied Biology, 4 juni 2007 N1 - Promotores : Olijve, W., Zoelen, E.J.J. van Co-promotor : Dechering, K.J. PS - 264 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/30023/30023.pdf?sequence=1 ER - TY - THES AU - Toepoel, M. PY - 2007 UR - https://hdl.handle.net/2066/30027 AB - Gene transcription is regulated by specific DNA sequences surrounding and within genes, so-called cis-acting factors, such as the promoter. Transcription factors or trans-acting factors, bind to these regulatory DNA elements in a sequence specific manner to influence the rate of transcription. Consequently, sequence variation in regulatory DNA elements, referred to as DNA polymorphisms, may alter protein binding and thus transcription of neighboring genes, and subsequently may also influence susceptibility to diseases. The gene encoding the platelet-derived growth factor receptor-alpha (PDGFRA) has been associated with a number of pathological conditions, such as neural tube defects (spina bifida) and various cancers including gliomas. The studies described in this thesis were undertaken to identify both cis- and trans-acting factors that regulate PDGFRA transcription and to determine their involvement in gliomas and NTDs. We identified ten polymorphisms in the human PDGFRA promoter, which together form five haplotypes: H1, H2alpha, H2beta, H2gamma and H2delta, of which H1and H2alpha are the most common. H1 mediates low, while H2alpha mediates high transcriptional activity. Our results indicate that this difference in activity can partly be regulated at the epigenetic level. From several case-control studies it appeared the low activity haplotype H1 was overrepresented in spina bifida cases, while it was underrepresented in glioblastoma cases. This suggests that low PDGFRA expression predisposes to neural tube defects, while at the same time protecting against gliomas. Furthermore, PDGFRA haplotypes appeared to differentially interact with maternal nutritional factors in predisposition to spina bifida, and also affected body growth. Finally, we identified a number of PDGFRA upstream regulators, some of which themselves have been directly associated with NTDs PB - Nijmegen : [S.n.] TI - PDGFRA transcriptional regulation in human physiology and disease N1 - Radboud University, Cell Biology, 22 mei 2007 N1 - Promotor : Zoelen, E.J.J. van PS - 132 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/30027/30027.pdf?sequence=1 ER - TY - THES AU - Alwan, H.A.J. PY - 2007 UR - https://hdl.handle.net/2066/30196 PB - Nijmegen : [S.n.] TI - UBPY-dependent deubiquitination as a means to control EGFR signaling N1 - Radboud university, Cell Biology, 11 juni 2007 N1 - Promotor : Zoelen, E.J.J. van PS - 113 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/30196/30196.pdf?sequence=1 ER - TY - JOUR AU - Hornberg, J.J. AU - Dekker, H.M. AU - Peters, P.H.J. AU - Langerak, P. AU - Westerhoff, H.V. AU - Lankelma, J. AU - Zoelen, E.J.J. van PY - 2006 UR - https://hdl.handle.net/2066/35867 TI - Epidermal Growth Factor Receptor-Induced Activator Protein 1 Activity Controls Density-Dependent Growth Inhibition in Normal Rat Kidney Fibroblasts EP - 108 SN - 1073-6085 IS - iss. 2 SP - 101 JF - Molecular Biotechnology VL - vol. 34 ER - TY - JOUR AU - Wingens, M. AU - Jacobs-Oomen, S. AU - Woning, S.P. van der AU - Stortelers, C. AU - Zoelen, E.J.J. van PY - 2006 UR - https://hdl.handle.net/2066/35868 TI - Epidermal growth factor mutant with wild-type affinity for both ErbB1 and ErbB3 EP - 4710 SN - 0006-2960 IS - iss. 14 SP - 4703 JF - Biochemistry VL - vol. 45 ER - TY - JOUR AU - Alwan, H.A.J. AU - Leeuwen, J.E.M. van PY - 2006 UR - https://hdl.handle.net/2066/34663 TI - UBPY-mediated EGFR deubiquitination promotes EGFR degradation EP - 1669 SN - 0021-9258 IS - iss. 3 SP - 1658 JF - Journal of Biological Chemistry ER - TY - JOUR AU - Verhoeckx, K.C. AU - Doornbos, R.P. AU - Witkamp, R.F. AU - Greef, J. van der AU - Rodenburg, R.J.T. PY - 2006 UR - https://hdl.handle.net/2066/36068 AB - Vascular endothelial growth factor (VEGF), oncostatin M (OSM), and granulocyte chemotactic protein-2 (GCP-2/CXCL6) are up-regulated in U937 macrophages and peripheral blood macrophages exposed to LPS, beta-adrenergic receptor (beta2-AR) agonists (e.g. zilpaterol, and clenbuterol) and some other agents that induce intracellular cAMP (prostaglandin E2, forskolin, and butyryl cAMP). LPS in combination with beta2-agonists and cAMP elevating agents had an additional effect on the release of VEGF, OSM, and CXCL6. These proteins are up-regulated after 16-24 h of exposure and this is mediated by the beta2-AR, as determined by time course experiments and the use of a specific beta2-AR antagonist (ICI 118551). Beta2-AR agonists are used as bronchodilators in the treatment of asthma, but appear to have no effect on the chronic inflammation of the disease. However, the up-regulation of VEGF, OSM, and CXCL6 may have adverse effects on the inflammatory process of asthma. These mediators are involved in the recruitment of neutrophils, airway remodelling and angiogenesis, known features of chronic inflammatory diseases. We propose that the up-regulation of these proteins could play a role in the adverse effects of prolonged excessive usage of beta2-AR agonists on the airways besides the desensitization of the beta2-AR. TI - Beta-adrenergic receptor agonists induce the release of granulocyte chemotactic protein-2, oncostatin M, and vascular endothelial growth factor from macrophages. EP - 7 SN - 1567-5769 IS - iss. 1 SP - 1 JF - International Immunopharmacology VL - vol. 6 DO - https://doi.org/10.1016/j.intimp.2005.05.013 ER - TY - JOUR AU - Damen, E. AU - Krieger, E. AU - Nielsen, J.E. AU - Eygensteyn, J. AU - Leeuwen, J.E.M. van PY - 2006 UR - https://hdl.handle.net/2066/35221 TI - The human Vps29 retromer component is a metallo-phophoesterase for a cation-independent mannose 6-phosphate receptor substrate peptide EP - 409 SN - 0264-6021 IS - iss. 3 SP - 399 JF - Biochemical Journal VL - vol. 398 DO - https://doi.org/10.1042/BJ20060033 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35221/35221.pdf?sequence=1 ER - TY - JOUR AU - Vaes, B.L.T. AU - Ducy, P. AU - Sijbers, A.M. AU - Hendriks, J.M.A. AU - Someren, E.P. van AU - Jong, N.G. de AU - Heuvel, E.R. van den AU - Olijve, W. AU - Zoelen, E.J.J. van PY - 2006 UR - https://hdl.handle.net/2066/35643 TI - Microarray analysis on Runx2-deficient mouse embryos reveals novel Runx2 functions and target genes during intramembranous and endochondral bone formation EP - 738 SN - 8756-3282 IS - iss. 4 SP - 724 JF - Bone VL - vol. 39 DO - https://doi.org/10.1016/j.bone.2006.04.024 ER - TY - JOUR AU - Woning, S.P. van der AU - Rotterdam, W. van AU - Nabuurs, S.B. AU - Venselaar, H. AU - Jacobs-Oomen, S. AU - Wingens, M. AU - Vriend, G. AU - Stortelers, C. AU - Zoelen, E.J.J. van PY - 2006 UR - https://hdl.handle.net/2066/35633 AB - Epidermal growth factor (EGF)-like growth factors bind their ErbB receptors in a highly selective manner, but the molecular basis for this specificity is poorly understood. We have previously shown that certain residues in human EGF (Ser(2)-Asp(3)) and TGFalpha (Glu(26)) are not essential for their binding to ErbB1 but prevent binding to ErbB3 and ErbB4. In the present study, we have used a phage display approach to affinity-optimize the C-terminal linear region of EGF-like growth factors for binding to each ErbB receptor and thereby shown that Arg(45) in EGF impairs binding to both ErbB3 and ErbB4. By omitting all these so-called negative constraints from EGF, we designed a ligand designated panerbin that binds ErbB1, ErbB3, and ErbB4 with similarly high affinity as their wild-type ligands. Homology models, based on the known crystal structure of TGFalpha-bound ErbB1, showed that panerbin is able to bind ErbB1, ErbB3, and ErbB4 in a highly similar manner with respect to position and number of interaction sites. Upon in silico introduction of the experimentally known negative constraints into panerbin, we found that Arg(45) induced local charge repulsion and Glu(26) induced steric hindrance in a receptor-specific manner, whereas Ser(2)-Asp(3) impaired binding due to a disordered conformation. Furthermore, radiolabeled panerbin was used to quantify the level of all three receptors on human breast cancer cells in a single radioreceptor assay. It is concluded that the ErbB specificity of EGF-like growth factors primarily results from the presence of a limited number of residues that impair the unintended interaction with other ErbB receptors. TI - Negative constraints underlie the ErbB specificity of epidermal growth factor-like ligands EP - 40040 SN - 1083-351X IS - iss. 52 SP - 40033 JF - Journal of Biological Chemistry VL - vol. 281 DO - https://doi.org/10.1074/jbc.M603168200 ER - TY - JOUR AU - Verhoeckx, K.C. AU - Gaspari, M. AU - Bijlsma, S. AU - Greef, J. van der AU - Witkamp, R.F. AU - Doornbos, R.P. AU - Rodenburg, R.J.T. PY - 2005 UR - https://hdl.handle.net/2066/32622 AB - Two-dimensional difference gel electrophoresis (DIGE) in combination with univariate (Student's t-test) and multivariate data analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to study the anti-inflammatory effects of the beta(2)-adrenergic receptor (beta(2)-AR) agonist zilpaterol. U937 macrophages were exposed to the endotoxin lipopolysaccharide (LPS) to induce an inflammatory reaction, which was inhibited by the addition of zilpaterol (LZ). This inhibition was counteracted by addition of the beta(2)-AR antagonist propranolol (LZP). The extracellular proteome of the U937 cells induced by the three treatments were examined by DIGE. PCA was used as an explorative tool to investigate the clustering of the proteome dataset. Using this tool, the dataset obtained from cells treated with LPS and LZP were separated from those obtained from LZ treated cells. PLS-DA, a multivariate data analysis tool that also takes correlations between protein spots and class assignment into account, correctly classified the different extracellular proteomes and showed that many proteins were differentially expressed between the proteome of inflamed cells (LPS and LZP) and cells in which the inflammatory response was inhibited (LZ). The Student's t-test revealed 8 potential protein biomarkers, each of which was expressed at a similar level in the LPS and LZP treated cells, but differently expressed in the LZ treated cells. Two of the identified proteins, macrophage inflammatory protein-1beta (MIP-1beta) and macrophage inflammatory protein-1alpha (MIP-1alpha) are known secreted proteins. The inhibition of MIP-1beta by zilpaterol and the involvement of the beta(2)-AR and cAMP were confirmed using a specific immunoassay. TI - In search of secreted protein biomarkers for the anti-inflammatory effect of beta2-adrenergic receptor agonists: application of DIGE technology in combination with multivariate and univariate data analysis tools. EP - 2023 SN - 1535-3893 IS - iss. 6 SP - 2015 JF - Journal of Proteome Research VL - vol. 4 DO - https://doi.org/10.1021/pr050183u ER - TY - JOUR AU - Verhoeckx, K.C. AU - Doornbos, R.P. AU - Greef, J. van der AU - Witkamp, R.F. AU - Rodenburg, R.J.T. PY - 2005 UR - https://hdl.handle.net/2066/32642 AB - In this study the anti-inflammatory properties of zilpaterol, a beta2-adrenergic receptor (AR) agonist specifically developed as a growth promoter in cattle were investigated. Although zilpaterol has a different structure compared with the beta2-AR agonists known to date, it was noted that it was able to bind to both the beta2-AR (Ki = 1.1 x 10(-6)) and the beta1-AR (Ki = 1.0 x 10(-5)). Using lipopolysaccharide (LPS)-exposed U937 macrophages, the production of cyclic adenosine-3',5'-cyclic monophosphate (cAMP) and tumor necrosis factor alpha (TNF-alpha) were investigated. Zilpaterol inhibited TNF-alpha release and induced intracellular cAMP levels in a dose-dependent manner. The inhibition of TNF-alpha release and induction of cAMP production was mainly mediated via the beta2-AR, as indicated by addition of beta1- and beta2-specific antagonists. The effects of zilpaterol were investigated in LPS-treated male Wistar rats after pretreatment with zilpaterol. Zilpaterol dosed at 500 microg/kg body weight reduced the TNF-alpha plasma levels. In conclusion, zilpaterol is a beta2-adrenergic agonist and an inhibitor of TNF-alpha production induced by LPS both in vivo and in vitro. TI - Inhibitory effects of the beta-adrenergic receptor agonist zilpaterol on the LPS-induced production of TNF-alpha in vitro and in vivo. EP - 537 SN - 0140-7783 IS - iss. 6 SP - 531 JF - Journal of Veterinary Pharmacology and Therapeutics VL - vol. 28 DO - https://doi.org/10.1111/j.1365-2885.2005.00691.x ER - TY - JOUR AU - van de Poll, M.L. AU - Rotterdam, W. van AU - Gadellaa, M.M. AU - Jacobs-Oomen, S. AU - Zoelen, E.J.J. van PY - 2005 UR - https://hdl.handle.net/2066/32682 AB - EGF activates the ErbB1 receptor, but there appears only a limited correlation between its receptor binding affinity and mitogenic activity. This is indicated by our present observation that in cells with high ErbB1 expression, including SUM102 breast tumor cells, low affinity EGF/Notch chimeras have similarly high mitogenic activity as EGF, in spite of the fact that EGF is superior in inducing receptor tyrosine phosphorylation and p42/p44 MAP-kinase activity. However, as a result of receptor-mediated internalisation high-affinity ligands such as EGF are depleted much more rapidly from the extracellular medium than low-affinity EGF/Notch chimeras. As a consequence, the mitogenic activity of EGF on ErbB1 overexpressing cells is limited by substantial degradation of internalised ligand in the period before cells enter S-phase, a phenomenon that is not observed for low affinity mutant ligands. The mitogenic activity of EGF on ErbB1 overexpressing cells does therefore not only depend on the applied concentration but also on the total amount of ligand added, and is strongly underestimated when tested in a limited assay volume. No such dependence on the incubation volume was observed for EGF activity on cells with low ErbB1 expression levels and on cells for which EGF is growth inhibitory. TI - Ligand depletion negatively controls the mitogenic activity of epidermal growth factor EP - 641 SN - 0014-4827 IS - iss. 2 SP - 630 JF - Experimental Cell Research VL - vol. 304 DO - https://doi.org/10.1016/j.yexcr.2004.12.011 ER - TY - JOUR AU - Joosten, P.H.L.J. AU - Zoelen, E.J.J. van AU - Murre, C. PY - 2005 UR - https://hdl.handle.net/2066/32833 AB - Many mouse models exist for neural tube defects (NTDs), but only few of them are relevant for human patients that are born alive with spina bifida aperta. NTDs in humans show a complex inheritance, which most likely result from the involvement of a variety of predisposing genetic and environmental factors. Hints toward the identity of predisposing genetic factors for human NTDs could come from mouse studies on the development of the neural tube and spinal cord, as well as from studies on associated features of this type of diseases. Among such features is the observation that pregnancies affected by a neural tube defect frequently show changes in thymus morphology, and in both neonatal and maternal T-cell repertoire. The genes for E2a and Pax1 have both been implicated in not only paraxial mesodermal development, but also in that of the immune system. Moreover, Pax1 mutant mice have been shown to display NTDs in digenic mouse models. In the present study we have investigated the phenotype of E2a null mutant mice that are also heterozygous for the so-called undulated mutation in Pax1. Here we report that such double-mutant mice develop a non-lethal NTD that strongly resembles the classic human NTD: spina bifida aperta, associated with defects of the axial skeleton, immune system and urinary tract. TI - Pax1/E2a Double-Mutant Mice Develop Non-Lethal Neural Tube Defects that Resemble Human Malformations EP - 987 SN - 0962-8819 IS - iss. 6 SP - 983 JF - Transgenic Research VL - vol. 14 DO - https://doi.org/10.1007/s11248-005-2540-9 ER - TY - JOUR AU - Vaes, B.L.T. AU - Dechering, K.J. AU - Someren, E.P. van AU - Hendriks, J.M. AU - Ven, C.J. van de AU - Feijen, A. AU - Mummery, C.L. AU - Reinders, M.J. AU - Olijve, W. AU - Zoelen, E.J.J. van AU - Steegenga, W.T. PY - 2005 UR - https://hdl.handle.net/2066/32739 AB - Wnt signaling has been implicated in regulating bone formation by controlling osteoblast proliferation and function. Although stabilization of beta-catenin by Wnt has been shown to increase alkaline phosphatase expression and osteoblast differentiation, the precise role of Wnt signaling during the process of osteoblast differentiation is largely unknown. In this study, we used microarray technology to investigate expression regulation of Wnt signaling components during in vitro osteoblast differentiation. Expression was analyzed during bone morphogenetic protein 2 (BMP2)-induced osteoblast differentiation of murine C2C12 and MC3T3 cells and data were compared with expression in BMP2-treated NIH3T3 fibroblasts. During osteoblast differentiation, particularly strong expression regulation of the Wnt antagonists Sfrp2 (secreted frizzled related protein 2) and Wif1 (Wnt inhibitory factor 1) was observed in the late phase of differentiation. In situ expression analysis in murine tail vertebrae supported Wif1 expression during late phase bone cell differentiation, since Wif1 was found to be expressed in vivo in trabecular, but not in cortical bone. We further analyzed the effects of continuous activation of Wnt signaling by lithium chloride and observed that osteoblast differentiation was reduced, as measured by expression of osteoblast marker genes encoding alkaline phosphatase, osteocalcin, and osterix, as well as by the amount of calcium release. Taken together, our data indicate that endogenous expression of Wnt antagonists by osteoblasts provides a negative Wnt feedback loop which is essential in controlling osteoblast maturation. TI - Microarray analysis reveals expression regulation of Wnt antagonists in differentiating osteoblasts EP - 811 SN - 8756-3282 IS - iss. 5 SP - 803 JF - Bone VL - vol. 36 DO - https://doi.org/10.1016/j.bone.2005.02.001 ER - TY - JOUR AU - Kusters, M.J.A.M. AU - Dernison, M.M. AU - Meerwijk, W.P. van AU - Ypey, D.L. AU - Theuvenet, A.P.R. AU - Gielen, C.C.A.M. PY - 2005 UR - https://hdl.handle.net/2066/33009 TI - Stabilizing Role of Calcium Store-Dependent Plasma Membrane Calcium Channels in Action-Potential Firing and Intracellular Calcium Oscillations EP - 3756 SN - 0006-3495 IS - iss. 6 SP - 3741 JF - Biophysical Journal VL - vol. 89 DO - https://doi.org/10.1529/biophysj.105.062984 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/33009/33009.pdf?sequence=1 ER - TY - JOUR AU - Someren, E.P. van AU - Vaes, B.L.T. AU - Steegenga, W.T. AU - Sijbers, A.M. AU - Dechering, K.J. AU - Reinders, M.J. PY - 2005 UR - https://hdl.handle.net/2066/32676 AB - MOTIVATION: We propose a reverse engineering scheme to discover genetic regulation from genome-wide transcription data that monitors the dynamic transcriptional response after a change in cellular environment. The interaction network is estimated by solving a linear model using simultaneous shrinking of the least absolute weights and the prediction error. RESULTS: The proposed scheme has been applied to the murine C2C12 cell-line stimulated to undergo osteoblast differentiation. Results show that our method discovers genetic interactions that display significant enrichment of co-citation in literature. More detailed study showed that the inferred network exhibits properties and hypotheses that are consistent with current biological knowledge. AVAILABILITY: Software is freely available for academic use as a Matlab package, called GENLAB: http://genlab.tudelft.nl/genlab.html. SUPPLEMENTARY INFORMATION: Additional data, results and figures can be found at http://genlab.tudelft.nl/larna.html. TI - Least Absolute Regression Network Analysis of the murine osteoblast differentiation network EP - 484 SN - 1367-4803 IS - iss. 4 SP - 477 JF - Bioinformatics VL - vol. 22 DO - https://doi.org/10.1093/bioinformatics/bti816 ER - TY - JOUR AU - Ottenheijm, C.A.C. AU - Heunks, L.M.A. AU - Sieck, G.C. AU - Zhan, W.Z. AU - Jansen, S.M. AU - Degens, H. AU - Boo, T.M. de AU - Dekhuijzen, P.N.R. PY - 2005 UR - https://hdl.handle.net/2066/32406 AB - RATIONALE: Hypercapnic respiratory failure because of inspiratory muscle weakness is the most important cause of death in chronic obstructive pulmonary disease (COPD). However, the pathophysiology of failure of the diaphragm to generate force in COPD is in part unclear. OBJECTIVES: The present study investigated contractile function and myosin heavy chain content of diaphragm muscle single fibers from patients with COPD. METHODS: Skinned muscle fibers were isolated from muscle biopsies from the diaphragm of eight patients with mild to moderate COPD and five patients without COPD (mean FEV(1) % predicted, 70 and 100%, respectively). Contractile function of single fibers was assessed, and afterwards, myosin heavy chain content was determined in these fibers. In diaphragm muscle homogenates, the level of ubiquitin-protein conjugation was determined. RESULTS: Diaphragm muscle fibers from patients with COPD showed reduced force generation per cross-sectional area, and reduced myosin heavy chain content per half sarcomere. In addition, these fibers had decreased Ca2+ sensitivity of force generation, and slower cross-bridge cycling kinetics. Our observations were present in fibers expressing slow and 2A isoforms of myosin heavy chain. Ubiquitin-protein conjugation was increased in diaphragm muscle homogenates of patients with mild to moderate COPD. CONCLUSIONS: Early in the development of COPD, diaphragm fiber contractile function is impaired. Our data suggest that enhanced diaphragm protein degradation through the ubiquitin-proteasome pathway plays a role in loss of contractile protein and, consequently, failure of the diaphragm to generate force. TI - Diaphragm dysfunction in chronic obstructive pulmonary disease. EP - 205 SN - 1073-449X IS - iss. 2 SP - 200 JF - American Journal of Respiratory and Critical Care Medicine VL - vol. 172 DO - https://doi.org/10.1164/rccm.200502-262OC ER - TY - JOUR AU - Harks, G.A. AU - Peters, P.H.J. AU - van Dongen, J.L. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2005 UR - https://hdl.handle.net/2066/33256 AB - We have used normal rat kidney (NRK) fibroblasts as an in vitro model system to study cell transformation. These cells obtain a transformed phenotype upon stimulation with growth-modulating factors such as retinoic acid (RA) or transforming growth factor-beta (TGF-beta). Patch-clamp experiments showed that transformation is paralleled by a profound membrane depolarization from around -70 to -20 mV. This depolarization is caused by a compound in the medium conditioned by transformed NRK cells, which enhances intracellular Ca2+ levels and thereby activates Ca2+-dependent Cl- channels. This compound was identified as prostaglandin F2alpha (PGF2alpha) using electrospray ionization mass spectrometry. The active concentration in the medium conditioned by transformed NRK cells as determined using an enzyme immunoassay was 19.7 +/- 2.5 nM (n = 6), compared with 1.5 +/- 0.1 nM (n = 3) conditioned by nontransformed NRK cells. Externally added PGF2alpha was able to trigger NRK cells that had grown to density arrest to restart their proliferation. This proliferation was inhibited when the FP receptor (i.e., natural receptor for PGF2alpha) was blocked by AL-8810. RA-induced phenotypic transformation of NRK cells was partially (approximately 25%) suppressed by AL-8810. Our results demonstrate that PGF2alpha acts as an autocrine enhancer and paracrine inducer of cell transformation and suggest that it may play a crucial role in carcinogenesis in general. TI - Autocrine production of prostaglandin F2alpha enhances phenotypic transformation of normal rat kidney fibroblasts EP - 7 SN - 0363-6143 IS - iss. 1 SP - C130 JF - American Journal of Physiology : Cell Physiology VL - vol. 289 DO - https://doi.org/10.1152/ajpcell.00416.2004 ER - TY - JOUR AU - Toepoel, M. AU - Ackerschott, B. AU - Zoelen, E.J.J. van PY - 2005 UR - https://hdl.handle.net/2066/32571 AB - We have previously shown that polymorphisms in the promoter of the human platelet-derived growth factor alpha-receptor (PDGFRA) gene can be grouped into five distinct haplotypes, designated H1, H 2 alpha, H 2 beta, H 2 gamma and H 2 delta, and that specific combinations of these promoter haplotypes predispose to neural tube defects (NTDs). These promoter haplotypes differ strongly in their ability to drive reporter gene expression in various human cell lines, with highest activity for H 2 alpha and H 2 beta. Here, we show that the haplotype-linked PDGFRA promoter region extends to 3.6 kb upstream from the transcription start site, and contains a total of ten polymorphic sites. For two of these polymorphic sites, i.e. -909 C/A and +68 GAins/del, we observed differential binding of nuclear proteins from human osteosarcoma (HOS) cells. The protein complex binding specifically to -909 C, which is present in all haplotypes except the low activity haplotype H 2 gamma, contained members of the upstream stimulatory factor (USF) family of transcription factors. Furthermore, we identified a protein complex of 125 kDa which bound specifically to the low activity haplotype H1 at position +68 GAdel and may represent an H1-specific PDGFRA transcriptional repressor. The current identification of cis-acting elements in the PDGFRA promoter and the transcription factors that bind them, provides a new strategy for the identification of genes that are potentially involved in neural tube defects. TI - Haplotype-dependent binding of nuclear proteins to the promoter of the neural tube defects-associated platelet-derived growth factor alpha-receptor gene EP - 357 SN - 0006-3002 IS - iss. 3 SP - 350 JF - Biochimica et Biophysica Acta VL - vol. 1741 ER - TY - THES AU - Jong, D.S. de PY - 2005 SN - 9090196943 UR - https://hdl.handle.net/2066/32614 PB - [S.l. : s.n.] TI - Identification of novel regulators involved in early phase osteoblast differentiation N1 - RU Radboud Universiteit Nijmegen, 26 oktober 2005 N1 - Promotores : Olijve, W., Zoelen, E.J.J. van PS - 156 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/32614/32614.pdf?sequence=1 ER - TY - JOUR AU - Nakagawa, T. AU - Li, J. AU - Garcia, G. AU - Mu, W. AU - Piek, E. AU - Bottinger, E.P. AU - Chen, Y. AU - Zhu, H.J. AU - Kang, D. AU - Schreiner, G.F. AU - Lan, H.Y. AU - Johnson, R.J. PY - 2004 UR - https://hdl.handle.net/2066/57438 AB - Background. Angiogenesis has a key role in numerous disease processes. One of the most important angiogenic factors is vascular endothelial growth factor (VEGF-A), whereas thrombospondin-1 (TSP-1) is a major antiangiogenic factor. Recent studies have shown that VEGF-A as well as TSP-1 is regulated by transforming growth factor-beta1 (TGF-beta1), but the mechanism remains unclear. Methods. We examined the role of TGF-beta1 and its signaling pathways in mediating expression of these two molecules. Rat proximal tubular cells (NRK52E) were stimulated with TGF-beta1 to induce VEGF-A and TSP-1 synthesis. To clarify roles of receptor-activated Smads (R-Smads), we blocked Smad signaling using overexpression of the inhibitory Smad, Smad7, and by using fibroblasts from wild-type or knockout mice. To confirm the antiantigenic role of Smads, soluble Flt-1 regulation in response to TGF-beta1 was also examined. In addition, the effect of conditioned media from NRK52E and Smad knockout cells was examined on endothelial cell proliferation. Results. Induction of VEGF-A and TSP-1 by TGF-beta1 in NRK52E cells was associated with activation of pathway-restricted R-Smads (Smad2 and 3) and blocking these Smads by overexpression of Smad7 blocked their induction. By using of Smad knockout cells, Smad3 was shown to have a key role in the stimulation of VEGF-A expression whereas Smad2 was critical for TSP-1 expression. Consistent with the hypothesis that Smad2 has an antiangiogenic function, we also demonstrated that Smad2, but not Smad3, mediated the expression of VEGF-A antagonist, soluble VEGF-A receptor sFlt-1, in response to TGF-beta1. Conditioned media from NRK52E, which was stimulated by TGF-beta1 for 24 hours, did not induce endothelial cell proliferation. However, conditioned media from Smad2 knockout induced endothelial cell proliferation, whereas endothelial cell proliferation was inhibited by Smad3 knockout-derived conditioned media. Conclusion. R-Smads have distinct roles in mediating the expression of pro- and antiangiogenic growth factors in response to TGF-beta1. TI - TGF-beta induces proangiogenic and antiangiogenic factors via parallel but distinct Smad pathways EP - 613 SN - 2157-1724 IS - iss. 2 SP - 605 JF - Kidney International. Supplement VL - vol. 66 DO - https://doi.org/10.1111/j.1523-1755.2004.00780.x ER - TY - JOUR AU - Torres, J.J. AU - Cornelisse, L.N. AU - Harks, E.G. AU - Meerwijk, W.P. van AU - Theuvenet, A.P.R. AU - Ypey, D.L. PY - 2004 UR - https://hdl.handle.net/2066/57337 AB - Normal rat kidney (NRK) fibroblasts change their excitability properties through the various stages of cell proliferation. The present mathematical model has been developed to explain excitability of quiescent (serum deprived) NRK cells. It includes as cell membrane components, on the basis of patch-clamp experiments, an inwardly rectifying potassium conductance (G(Kir)), an L-type calcium conductance (G(CaL)), a leak conductance (G(leak)), an intracellular calcium-activated chloride conductance [G(Cl(Ca))], and a gap junctional conductance (G(gj)), coupling neighboring cells in a hexagonal pattern. This membrane model has been extended with simple intracellular calcium dynamics resulting from calcium entry via G(CaL) channels, intracellular buffering, and calcium extrusion. It reproduces excitability of single NRK cells and cell clusters and intercellular action potential (AP) propagation in NRK cell monolayers. Excitation can be evoked by electrical stimulation, external potassium-induced depolarization, or hormone-induced intracellular calcium release. Analysis shows the roles of the various ion channels in the ultralong ( approximately 30 s) NRK cell AP and reveals the particular role of intracellular calcium dynamics in this AP. We support our earlier conclusion that AP generation and propagation may act as a rapid mechanism for the propagation of intracellular calcium waves, thus contributing to fast intercellular calcium signaling. The present model serves as a starting point to further analyze excitability changes during contact inhibition and cell transformation. TI - Modeling action potential generation and propagation in NRK fibroblasts. EP - 65 SN - 0363-6143 IS - iss. 4 SP - C851 JF - American Journal of Physiology : Cell Physiology VL - vol. 287 DO - https://doi.org/10.1152/ajpcell.00220.2003 ER - TY - JOUR AU - Jong, D.S. de AU - Vaes, B.L.T. AU - Dechering, K.J. AU - Feijen, A. AU - Hendriks, J.M. AU - Wehrens, H.R.M.J. AU - Mummery, C.L. AU - Zoelen, E.J.J. van AU - Olijve, W. AU - Steegenga, W.T. PY - 2004 UR - https://hdl.handle.net/2066/58213 AB - Key regulatory components of the BMP-induced osteoblast differentiation cascade remain to be established. Microarray and subsequent expression analyses in mice identified two transcription factors, Hey1 and Tcf7, with in vitro and in vivo expression characteristics very similar to Cbfa1. Transfection studies suggest that Tcf7 modulates BMP2-induced osteoblast differentiation. This study contributes to a better definition of the onset of BMP-induced osteoblast differentiation. INTRODUCTION: Elucidation of the genetic cascade guiding mesenchymal stem cells to become osteoblasts is of extreme importance for improving the treatment of bone-related diseases such as osteoporosis. The aim of this study was to identify regulators of the early phases of bone morphogenetic protein (BMP)2-induced osteoblast differentiation. MATERIALS AND METHODS: Osteoblast differentiation of mouse C2C12 cells was induced by treatment with BMP2, and regulation of gene expression was studied during the subsequent 24 h using high-density microarrays. The regulated genes were grouped by means of model-based clustering, and protein functions were assigned. Real-time quantitative RT-PCR analysis was used to validate BMP2-induced gene expression patterns in C2C12 cells. Osteoblast specificity was studied by comparing these expression patterns with those in C3H10T1/2 and NIH3T3 cells under similar conditions. In situ hybridization of mRNA in embryos at embryonic day (E)14.5 and E16.5 of gestation and on newborn mouse tails were used to study in vivo expression patterns. Cells constitutively expressing the regulated gene Tcf7 were used to investigate its influence on BMP-induced osteoblast differentiation. RESULTS AND CONCLUSIONS: A total of 184 genes and expressed sequence tags (ESTs) were differentially expressed in the first 24 h after BMP2 treatment and grouped in subsets of immediate early, intermediate early, and late early response genes. Signal transduction regulatory factors mainly represented the subset of immediate early genes. Regulation of expression of these genes was direct, independent of de novo protein synthesis and independent of the cell type studied. The intermediate early and late early genes consisted primarily of genes related to processes that modulate morphology, basement membrane formation, and synthesis of extracellular calcified matrix. The late early genes require de novo protein synthesis and show osteoblast specificity. In vivo and in vitro experiments showed that the transcription factors Hey1 and Tcf7 exhibited expression characteristics and cell type specificity very similar to those of the osteoblast specific transcription factor Cbfa1, and constitutive expression of Tcf7 in C2C12 cells differentially regulated osteoblast differentiation marker genes. TI - Identification of novel regulators associated with early-phase osteoblast differentiation. EP - 958 SN - 0884-0431 IS - iss. 6 SP - 947 JF - Journal of Bone and Mineral Research VL - vol. 19 DO - https://doi.org/10.1359/JBMR.040216 ER - TY - JOUR AU - Rampaart, L.J.A. AU - Camina, J.P. AU - Beekwilder, J.P. AU - Harks, G.A. AU - Dernison, M.M. AU - Peters, P.H.J. AU - VanMeerwijk, M.P.M. AU - Theuvenet, A.P.R. AU - Ypey, D.L. PY - 2004 UR - https://hdl.handle.net/2066/59365 TI - The local anesthetic n-Butyl-p-AminoBenzoate (BAB) blocks gap junctional channels in fibroblastic normal rat kidney (NRK) cells EP - 584A SN - 0006-3495 IS - iss. 1 SP - 584A JF - Biophysical Journal VL - vol. 86 ER - TY - JOUR AU - Harks, G.A. AU - Peters, P.H.J. AU - Dongen, J. Van AU - Dernison, M.M. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2004 UR - https://hdl.handle.net/2066/57697 TI - Activation of FP-prostanoid receptors is involved in phenotypic transformation and concomitant depolarization of NRK fibroblasts EP - 459A SN - 0006-3495 IS - iss. 1 SP - 459A JF - Biophysical Journal VL - vol. 86 ER - TY - JOUR AU - Jong, D.S. de AU - Steegenga, W.T. AU - Hendriks, J.M. AU - Zoelen, E.J.J. van AU - Olijve, W. AU - Dechering, K.J. PY - 2004 UR - https://hdl.handle.net/2066/57750 AB - The bone morphogenetic protein (BMP)-induced Smad signal transduction pathway is an important positive regulator of osteoblast differentiation. BMP and other members of the transforming growth factor-beta (TGF-beta) family have distinct effects on osteoblast differentiation, depending on cell type and cell differentiation status. In C2C12 mesenchymal cells, BMP-induced osteoblast differentiation can be blocked by TGF-beta. In a search for key regulators of osteoblast differentiation we have used microarray analysis to identify genes which are differentially regulated by BMP2 and TGF-beta. Within the first 24 h following the onset of differentiation, 61 BMP2-regulated genes were identified of which the BMP2 effect was counteracted by TGF-beta. The majority of these differentially expressed transcripts are related to signal transduction. Notably, our data show that three Notch signal transduction pathway genes, Lfng, Hey1, and Hes1, are differentially regulated by BMP2 and TGF-beta. This suggests that these genes might function as the focal point for interaction of Smad and Notch signaling during osteoblast differentiation. TI - Regulation of Notch signaling genes during BMP2-induced differentiation of osteoblast precursor cells. EP - 107 SN - 0006-291X IS - iss. 1 SP - 100 JF - Biochemical and Biophysical Research Communications VL - vol. 320 DO - https://doi.org/10.1016/j.bbrc.2004.05.150 ER - TY - JOUR AU - Kusters, M.J.A.M. AU - Theuvenet, A.P.R. AU - Ypey, D.L. AU - Gielen, C.C.A.M. PY - 2004 UR - https://hdl.handle.net/2066/57376 TI - Coupling of action potential firing and Ca-oscillations in a monolayer of gap junctionally coupled NRK fibroblasts EP - 303A SN - 0006-3495 IS - iss. 1 SP - 303A JF - Biophysical Journal VL - vol. 86 ER - TY - JOUR AU - Brouwers, A.A.M. AU - Vermeij-Keers, C. AU - Zoelen, E.J.J. van AU - Gooren, L.J.G. PY - 2004 UR - https://hdl.handle.net/2066/57808 AB - Clubbed digits resemble the human embryonic fingers and toes, which took like the digits of a claw. Clubbed digits, thus, may represent the return of the embryonic claw and may even represent the claws man has lost during evolution, if ontogenesis realty recapitulates phylogenesis. We put forward the hypothesis that secondary clubbing, Like gynecomastia, is caused by a pathologic condition, which alters hormone levels in the blood, leading to the activation of 'dormant' genes, resulting in the development of an organ. However, the nature of the diseases that cause clubbing suggests that these hormones may actually be cytokines, acting as hormones. The nature of these cytokines is not known. They may be identified by comparing their blood levels or the combination of their blood levels to the presence or absence of clubbing, but also to the degree of clubbing and its disappearance after treatment of the primary disease. (C) 2003 Elsevier Ltd. All rights reserved. TI - Clubbed fingers: the claws we lost? EP - 324 SN - 0306-9877 IS - iss. 3 SP - 321 JF - Medical Hypotheses VL - vol. 62 DO - https://doi.org/10.1016/S0306-9877(03)00300-1 ER - TY - JOUR AU - Torres, J.J. AU - Cornelisse, L.N. AU - Harks, G.A. AU - Theuvenet, A.P.R. AU - Ypey, D.L. PY - 2003 UR - https://hdl.handle.net/2066/124039 TI - Modelling action potential generation and propagation in normal rat kidney fibroblasts. EP - 865 SN - 0363-6143 IS - iss. 4 SP - 851 JF - American Journal of Physiology : Cell Physiology VL - vol. 287 ER - TY - JOUR AU - Harks, G.A. AU - Torres, J.J. AU - Cornelisse, L.N. AU - Ypey, D.L. AU - Theuvenet, A.P.R. PY - 2003 UR - https://hdl.handle.net/2066/124034 TI - Ionic basis for excitability of normal rat kidney (NRK) fibroblasts. EP - 503 SN - 0021-9541 SP - 493 JF - Journal of Cellular Physiology VL - vol. 196 DO - https://doi.org/10.1002/jcp.10346 ER - TY - JOUR AU - Camina, J.P. AU - Diaz-Rodriguez, E. AU - Harks, G.A. AU - Theuvenet, A.P.R. AU - Ypey, D.L. AU - Casanueva, F.F. PY - 2003 UR - http://repository.ubn.ru.nl/handle/2066/127208 TI - Lipid factor (bVLF) from bovine vitreous body evokes in EGFR-T17 cells a Ca2+-dependent K+ current associated with inositol 1,4,5,-triphosphate-independent Ca2+ mobilization EP - 118 SN - 0021-9541 SP - 108 JF - Journal of Cellular Physiology VL - vol. 195 DO - https://doi.org/10.1002/jcp.10233 ER - TY - JOUR AU - Harks, G.A. AU - Camina, J.P. AU - Peters, P.H.J. AU - Ypey, D.L. AU - Scheenen, W.J.J.M. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2003 UR - https://hdl.handle.net/2066/129295 TI - Besides affecting intracellular calcium signaling, 2-APB reversibly blocks gap junctional coupling in confluent monolayers, thereby allowing the measurement of single-cell membrane currents in undissociated cells EP - 943 SN - 0892-6638 SP - 941 JF - The Faseb Journal VL - vol. 17 DO - https://doi.org/10.1096/fj.02-0786fje ER - TY - JOUR AU - Poorter, L.M.I. de AU - Geerts, W.G. AU - Theuvenet, A.P.R. AU - Keltjens, J.T.M. PY - 2003 UR - https://hdl.handle.net/2066/129296 TI - Bioenergetics of the formyl-methanofuran dehydrogenase and heterodisulfide reductase reactions in Methanothermobacter thermautotrophicus EP - 75 SN - 0014-2956 SP - 66 JF - European Journal of Biochemistry VL - vol. 270 DO - https://doi.org/10.1046/j.1432-1033.2003.03362.x ER - TY - JOUR AU - Wanka, F. AU - Zoelen, E.J.J. van PY - 2003 UR - https://hdl.handle.net/2066/129300 TI - Force generation by cellular motors EP - 1033 SN - 1425-8153 SP - 1017 JF - Cellular & Molecular Biology Letters VL - vol. 8 ER - TY - JOUR AU - Alwan, H.A.J. AU - Zoelen, E.J.J. van AU - Leeuwen, J.E.M. van PY - 2003 UR - https://hdl.handle.net/2066/129301 TI - Ligand-induced lysosomal epidermal growth factor receptor (EGFR) degradation is preceded by proteasome-dependent EGFR de-ubiquitination EP - 35790 SN - 1083-351X SP - 35781 JF - Journal of Biological Chemistry VL - vol. 278 DO - https://doi.org/10.1074/jbc.M301326200 ER - TY - JOUR AU - Looijenga, L.H.J. AU - Stoop, H. AU - Leeuw, H.P.J.C. de AU - Gouveia Brazao, C.A. De AU - Gillis, A.J.M. AU - Roozendaal, C.E.P. van AU - Zoelen, E.J.J. van AU - Weber, R.F.A. AU - Wolffenbuttel, K.P. AU - Dekken, H. van AU - Honecker, F. AU - Bokemeyer, C. AU - Perlman, E.J. AU - Kononen, J. AU - Sauter, G. AU - Oosterhuis, J.W. PY - 2003 UR - https://hdl.handle.net/2066/129302 TI - POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors EP - 2250 SN - 0008-5472 SP - 2244 JF - Cancer Research VL - vol. 63 ER - TY - JOUR AU - Harks, G.A. AU - Scheenen, W.J.J.M. AU - Peters, P.H.J. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2003 UR - https://hdl.handle.net/2066/129303 TI - Prostaglandin F2alpha induces unsynchronized intracellular calcium oscillations in monolayers of gap junctionally coupled NRK fibroblasts EP - 86 SN - 0031-6768 SP - 78 JF - Pflügers Archiv : European Journal of Physiology VL - vol. 447 DO - https://doi.org/10.1007/s00424-003-1126-8 ER - TY - JOUR AU - Stortelers, C. AU - Woning, S.P. van der AU - Jacobs-Oomen, S. AU - Wingens, M. AU - Zoelen, E.J.J. van PY - 2003 UR - https://hdl.handle.net/2066/129304 TI - Selective formation of ErbB-2/ErbB-3 heterodimers depends on the ErbB-3 affinity of epidermal growth factor-like ligands EP - 12063 SN - 1083-351X SP - 12055 JF - Journal of Biological Chemistry VL - vol. 278 DO - https://doi.org/10.1074/jbc.M211948200 ER - TY - JOUR AU - Wingens, M. AU - Walma, T. AU - Ingen, H. van AU - Stortelers, C. AU - Leeuwen, J.E.M. van AU - Zoelen, E.J.J. van AU - Vuister, G.W. PY - 2003 UR - https://hdl.handle.net/2066/79479 TI - Structural analysis of an epidermal growth factor/transforming growth factor-alpha chimera with unique ErbB binding specificity EP - 39123 SN - 1083-351X IS - iss. 40 SP - 39114 JF - Journal of Biological Chemistry VL - vol. 278 DO - https://doi.org/10.1074/jbc.M305603200 ER - TY - JOUR AU - Wanka, F. AU - Zoelen, E.J.J. van PY - 2003 UR - https://hdl.handle.net/2066/129298 TI - Cellular organelle transport and positioning by plasma streaming EP - 1045 SN - 1425-8153 SP - 1035 JF - Cellular & Molecular Biology Letters VL - vol. 8 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/129298/129298.pdf?sequence=1 ER - TY - JOUR AU - Hooge, A.S.K. de AU - Loo, F.A.J. van de AU - Bennink, M.B. AU - Jong, D.S. de AU - Arntz, O.J. AU - Lubberts, G.J.H. AU - Richards, C.D. AU - Berg, W.B. van den PY - 2002 UR - https://hdl.handle.net/2066/142117 AB - Oncostatin M (OSM) has been described as a bone-remodeling factor either stimulating osteoblast activity or osteoclast formation in vitro. To elucidate the in vivo effect of OSM on bone remodeling, we injected an adenoviral vector encoding murine OSM in knee joints of mice. OSM strongly induced interleukin (IL)-6 gene expression, a known mediator of osteoclast development. We investigated the OSM effect in wild-type and IL-6-deficient mice and found a similar degree of OSM-induced joint inflammation. Within the first week of inflammation, the periosteum along the femur and tibia increased in cell number and stained positive for the osteoblast marker alkaline phosphatase. At these sites bone apposition occurred in both strains as demonstrated by Goldner and Von Kossa staining. In vitro OSM enhanced the effect of bone morphogenetic protein-2 on osteoblast differentiation. Immunohistochemistry demonstrated expression of receptor activator of nuclear factor-kappa B ligand (RANKL) and its receptor, receptor activator of nuclear factor-kappa B (RANK), in the periosteum but osteoclasts were not detected at sites of bone apposition. Induced mRNA expression for the receptor activator of nuclear factor-kappa B ligand inhibitor osteoprotegerin probably controlled osteoclast development during OSM overexpression. Our results show that OSM favors bone apposition at periosteal sites instead of resorption in vivo. This effect was not dependent on or inhibited by IL-6. TI - Adenoviral transfer of murine oncostatin M elicits periosteal bone apposition in knee joints of mice, despite synovial inflammation and up-regulated expression of interleukin-6 and receptor activator of nuclear factor-kappa B ligand. EP - 1743 SN - 0002-9440 IS - iss. 5 SP - 1733 JF - American Journal of Pathology VL - vol. 160 DO - https://doi.org/10.1016/S0002-9440(10)61120-0 ER - TY - JOUR AU - Joosten, P.H.L.J. AU - Toepoel, M. AU - Oosterhout, D. van AU - Afink, G.B. AU - Zoelen, E.J.J. van PY - 2002 UR - https://hdl.handle.net/2066/129306 TI - A regulating element essential for PDGRFA transcription is recognized by neural tube defect-associated PRX homeobox transcription factors EP - 260 SN - 0925-4439 SP - 254 JF - Biochimica et Biophysica Acta. Molecular Basis of Disease VL - vol. 1588 DO - https://doi.org/10.1016/S0925-4439(02)00175-8 ER - TY - JOUR AU - Vaes, B.L.T. AU - Dechering, K.J. AU - Feijen, A. AU - Hendriks, J.M.A. AU - Lefevre, C. AU - Mummery, C.L. AU - Olijve, W. AU - Zoelen, E.J.J. van AU - Steegenga, W.T. PY - 2002 UR - https://hdl.handle.net/2066/129307 TI - Comprehensive microarray analysis of bone morphogenetic protein 2-induced osteoblast differentiation resulting in the identification of novel markers for bone development EP - 2118 SN - 0884-0431 SP - 2106 JF - Journal of Bone and Mineral Research VL - vol. 17 DO - https://doi.org/10.1359/jbmr.2002.17.12.2106 ER - TY - JOUR AU - Stortelers, C. AU - Poll, M.L.M. van de AU - Lenferink, A.E.G. AU - Gadellaa, M.M. AU - Zoelen, C. van AU - Zoelen, E.J.J. van PY - 2002 UR - https://hdl.handle.net/2066/129310 TI - Epidermal growth factor contains both positive and negative determinants for interaction with ErbB2/ErbB-3 heterodimers EP - 4301 SN - 0006-2960 SP - 4292 JF - Biochemistry VL - vol. 41 DO - https://doi.org/10.1021/bi012016n ER - TY - JOUR AU - Palumbo, C. AU - Roozendaal, K. van AU - Gillis, A.J.M. AU - Gurp, R.J.H.L.M. van AU - Munnik, H. de AU - Oosterhuis, J.W. AU - Zoelen, E.J.J. van AU - Looijenga, L.H.J. PY - 2002 UR - https://hdl.handle.net/2066/129311 TI - Expression of the PDGF (-receptor 1.5 kb transcript, OCT-4), and c-KIT in human normal and malignant tissues. Implications for the early diagnosis of testicular germ cell tumours and for our understanding of regulatory mechanisms EP - 477 SN - 0022-3417 SP - 467 JF - Journal of Pathology VL - vol. 196 DO - https://doi.org/10.1002/path.1064 ER - TY - JOUR AU - Stortelers, C. AU - Souriau, C. AU - Liempt, E. van AU - Poll, M.L.M. van de AU - Zoelen, E.J.J. van PY - 2002 UR - https://hdl.handle.net/2066/129314 TI - Role of the N-terminus of epidermal growth factor in ErbB-2/ErbB-3 binding studied by phage display EP - 8741 SN - 0006-2960 SP - 8732 JF - Biochemistry VL - vol. 41 DO - https://doi.org/10.1021/bi025878c ER - TY - JOUR AU - Harks, G.A. AU - Roos, A.D.G. de AU - Peters, P.H.J. AU - Haan, L. de AU - Brouwer, A.P.M. de AU - Ypey, D.L. AU - Zoelen, E.J.J. van AU - Theuvenet, A.P.R. PY - 2001 UR - https://hdl.handle.net/2066/185645 AB - The effect of fenamates on gap junctional intercellular communication was investigated in monolayers of normal rat kidney (NRK) fibroblasts and of SKHep1 cells overexpressing the gap junction protein connexin43 (Cx43). Using two different methods to study gap junctional intercellular communication, single electrode voltage-clamp step response measurements and dye microinjection, we show that fenamates are reversible blockers of Cx43-mediated intercellular communication. After adding fenamates to a confluent monolayer of electrically coupled NRK fibroblasts, the voltage step-induced capacitive current transient changed from a transient characteristic for charging multiple coupled cell capacitances to one characteristic for a single cell in isolation. The capacitance of completely uncoupled cells was 19.7 +/- 1.0 pF (mean +/- S.E.M.; n = 11). Junctional conductance between the patched cell and the surrounding cells in the monolayer changed from >140.7 +/- 9.6 nS (mean +/- S.E.M.; n = 14) to <1.4 +/- 0.4 nS (mean +/- S.E.M.; n = 11) after uncoupling. Electrical coupling could be restored to >51.8 +/- 4.2 nS (mean +/- S.E.M.; n = 11) by washout of the fenamates. Voltage-clamp step response measurements showed that the potency of fenamates in inhibiting electrical coupling decreases in the order meclofenamic acid > niflumic acid > flufenamic acid. The half-maximal concentration determined by dye-coupling experiments was 25 and 40 microM for meclofenamic acid and flufenamic acid, respectively. Inhibition of gap junctional communication by fenamates did not involve changes in intracellular calcium or pH, and was unrelated to protein kinase C activity or an inhibition of cyclooxygenase activity. Voltage-clamp step response measurements in confluent monolayers of SKHep1 cells that had been stably transfected with Cx43 revealed that fenamates are potent blockers of Cx43-mediated intercellular communication. In conclusion, fenamates represent a novel class of reversible gap junction blockers that can be used to study the role of Cx43-mediated gap junctional intercellular communication in biological processes. 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PY - 1999 UR - https://hdl.handle.net/2066/129338 TI - Functional characterization of two promoters in the human bone morphogenetic protein-4 gene EP - 1441 SN - 0884-0431 SP - 1432 JF - Journal of Bone and Mineral Research VL - vol. 14 DO - https://doi.org/10.1359/jbmr.1999.14.8.1432 ER - TY - JOUR AU - Laat, S.W. de AU - Boonstra, J.J. AU - Defize, L.H.K. AU - Kruijer, W. AU - Saag, P.T. van der AU - Tertoolen, L.F.J. AU - Zoelen, E.J.J. van AU - Hertog, J.F. den PY - 1999 UR - https://hdl.handle.net/2066/129339 TI - Growth factor signalling EP - 691 SN - 0214-6282 SP - 681 JF - International Journal of Developmental Biology VL - vol. 43 ER - TY - JOUR AU - Boersma, C.J.C. AU - Bloemen, M. AU - Hendriks, J.M.A. AU - Berkel, E.A.T. van AU - Olijve, W. 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PY - 1995 UR - https://hdl.handle.net/2066/197651 TI - Fine mapping of the human bone morphogenetic protein-4 (BMP-4) gene to chromosome 14q22-q23 by in situ hybridization EP - 560 SN - 0888-7543 SP - 559 JF - Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes VL - vol. 27 DO - https://doi.org/10.1006/geno.1995.1096 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/197651/197651.pdf?sequence=1 ER - TY - JOUR AU - Poll, M.L.M. van de AU - Lenferink, A.E.G. AU - Vugt, A.H.M. van AU - Jacobs, J.J.L. AU - Janssen, J.W.H. AU - Joldersma, M. AU - Zoelen, E.J.J. van PY - 1995 UR - https://hdl.handle.net/2066/216426 TI - A single amino acid exchange, Arg 45 to Ala, generates an epidermal growth facor (EGF) mutant with high affinity for the chicken EGF receptor EP - 22343 SN - 1083-351X SP - 22337 JF - Journal of Biological Chemistry VL - vol. 270 DO - https://doi.org/10.1074/jbc.270.38.22337 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/216426/216426.pdf?sequence=1 ER - TY - GEN AU - Jansen, S.M. AU - Kuyper, de A.L.C.M. PY - 1975 UR - https://hdl.handle.net/2066/222283 AB - Changes in primary educational system / attitude to reorganization / traditional teaching, individual freedom of the teacher, role perception / attitude to specific aspects of new style in teaching / use of educational appliances / opinion about headmaster / topics at meetings with colleagues / the aims of primary education / attitude to christian schools / perception of reasons why own teaching is not up to date. Background variables: basic characteristics/ place of work/ education/ organizational membership PB - DANS EASY TI - Onderwijzers werkzaam in het Katholiek basis onderwijs 1970~Teachers in Roman-Catholic primary education 1970 DO - https://doi.org/10.17026/dans-xdr-27gf ER -