TY - THES AU - Meijer, I.M.J. PY - 2014 SN - 97890902 UR - https://hdl.handle.net/2066/122889 PB - [S.l. : s.n.] TI - Ubiquitination and deubiquitination of ERBB receptors N1 - Radboud Universiteit Nijmegen, 24 januari 2014 N1 - Promotor : Zoelen, E.J.J. van Co-promotor : Leeuwen, J.E.M. van PS - 223 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/122889/122889.pdf?sequence=1 ER - TY - THES AU - Dernison, M.M. PY - 2012 SN - 9789461914125 UR - https://hdl.handle.net/2066/100592 PB - [S.l. : s.n.] TI - Pacemaking activity in normal rat kidney fibroblasts N1 - Radboud Universiteit Nijmegen, 13 november 2012 N1 - Promotores : Zoelen, E.J.J. van, Gielen, C.C.A.M. Co-promotor : Theuvenet, A.P.R. PS - 190 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/100592/100592.pdf?sequence=1 ER - TY - THES AU - Damen, E. PY - 2010 SN - 9789090254098 UR - https://hdl.handle.net/2066/77567 PB - [S.l. : s.n.] TI - The role of the retromer complex in receptor trafficking N1 - RU Radboud Universiteit Nijmegen, 5 juli 2010 PS - 245 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/77567/77567.pdf?sequence=1 ER - TY - THES AU - Woning, S.P. van der PY - 2010 SN - 9789090249636 UR - https://hdl.handle.net/2066/74880 PB - [S.l. : s.n.] TI - Molecular mechanisms that underlie the receptor specificity of ErbB ligands N1 - RU Radboud Universiteit Nijmegen, 12 januari 2010 PS - 243 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/74880/74880.pdf?sequence=1 ER - TY - THES AU - Vaes, B.L.T. PY - 2007 SN - 9789090218533 UR - https://hdl.handle.net/2066/30023 AB - The frequently occurring bone disorder osteoporosis is characterized by a strong increase in bone fracture risk, caused by a dramatically disturbed balance in the activity of the cells that degrade bone (osteoclasts) and cells that synthesize new bone (osteoblasts). Therapies against osteoporosis are currently based on inhibition of osteoclast activity in order to reduce the amount of bone resorption. An alternative strategy would be to stimulate new bone formation by enhancing the activity of osteoblasts. The main objective of this thesis was to identify genes of which expression and function are specifically associated with the differentiation of mesenchymal stem cells into osteoblasts. Therefore, we used microarray-based gene expression profiling to study the transcriptome of differentiating osteoblasts during in vitro cell culture as well as in their physiological context during skeletogenesis. The analyses revealed many genes of which expression in osteoblasts had not previously been detected. Novel markers of osteoblast differentiation and bone development were identified as well as putative in vivo target genes of the transcription factor Runx2 which is a key regulator of skeletal development. One of the markers identified encodes the protein Wif1 (Wnt Inhibitory Factor 1), which was shown to be specifically expressed in osteoblasts during embryonic development. Typically, genes encoding antagonists of the Wnt signalling pathway were strongly expressed during late stages of osteoblast differentiation and implicate an important role of Wnt antagonism in the maturation of osteoblasts. Given the role of Wnt signalling in the maintenance of bone mass, this class of proteins may be potential drug targets to treat bone disorders. This thesis shows that the combination of microarray technology and bioinformatic analyses has provided new possibilities to the understanding of bone biology. Consequently, our studies have contributed to a more detailed knowledge on osteoblast differentiation and provide focus points to develop strategies that will prevent and treat bone disorders. PB - Nijmegen : [S.n.] TI - Exploration of the mouse osteoblast transcriptome N1 - Radboud University, Applied Biology, 4 juni 2007 N1 - Promotores : Olijve, W., Zoelen, E.J.J. van Co-promotor : Dechering, K.J. PS - 264 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/30023/30023.pdf?sequence=1 ER - TY - THES AU - Toepoel, M. PY - 2007 UR - https://hdl.handle.net/2066/30027 AB - Gene transcription is regulated by specific DNA sequences surrounding and within genes, so-called cis-acting factors, such as the promoter. Transcription factors or trans-acting factors, bind to these regulatory DNA elements in a sequence specific manner to influence the rate of transcription. Consequently, sequence variation in regulatory DNA elements, referred to as DNA polymorphisms, may alter protein binding and thus transcription of neighboring genes, and subsequently may also influence susceptibility to diseases. The gene encoding the platelet-derived growth factor receptor-alpha (PDGFRA) has been associated with a number of pathological conditions, such as neural tube defects (spina bifida) and various cancers including gliomas. The studies described in this thesis were undertaken to identify both cis- and trans-acting factors that regulate PDGFRA transcription and to determine their involvement in gliomas and NTDs. We identified ten polymorphisms in the human PDGFRA promoter, which together form five haplotypes: H1, H2alpha, H2beta, H2gamma and H2delta, of which H1and H2alpha are the most common. H1 mediates low, while H2alpha mediates high transcriptional activity. Our results indicate that this difference in activity can partly be regulated at the epigenetic level. From several case-control studies it appeared the low activity haplotype H1 was overrepresented in spina bifida cases, while it was underrepresented in glioblastoma cases. This suggests that low PDGFRA expression predisposes to neural tube defects, while at the same time protecting against gliomas. Furthermore, PDGFRA haplotypes appeared to differentially interact with maternal nutritional factors in predisposition to spina bifida, and also affected body growth. Finally, we identified a number of PDGFRA upstream regulators, some of which themselves have been directly associated with NTDs PB - Nijmegen : [S.n.] TI - PDGFRA transcriptional regulation in human physiology and disease N1 - Radboud University, Cell Biology, 22 mei 2007 N1 - Promotor : Zoelen, E.J.J. van PS - 132 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/30027/30027.pdf?sequence=1 ER - TY - THES AU - Alwan, H.A.J. PY - 2007 UR - https://hdl.handle.net/2066/30196 PB - Nijmegen : [S.n.] TI - UBPY-dependent deubiquitination as a means to control EGFR signaling N1 - Radboud university, Cell Biology, 11 juni 2007 N1 - Promotor : Zoelen, E.J.J. van PS - 113 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/30196/30196.pdf?sequence=1 ER - TY - THES AU - Jong, D.S. de PY - 2005 SN - 9090196943 UR - https://hdl.handle.net/2066/32614 PB - [S.l. : s.n.] TI - Identification of novel regulators involved in early phase osteoblast differentiation N1 - RU Radboud Universiteit Nijmegen, 26 oktober 2005 N1 - Promotores : Olijve, W., Zoelen, E.J.J. van PS - 156 p. L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/32614/32614.pdf?sequence=1 ER -