TY - JOUR AU - Onnekink, C. AU - Kappel, R.M. AU - Boelens, Wilbert C. AU - Pruijn, G. PY - 2020 UR - https://hdl.handle.net/2066/224930 TI - Low molecular weight silicones induce cell death in cultured cells EP - 12 SN - 2045-2322 SP - 1 JF - Scientific Reports VL - vol. 10 DO - https://doi.org/10.1038/s41598-020-66666-7 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/224930/224930.pdf?sequence=1 ER - TY - JOUR AU - Kissel, T. AU - Reijm, S. AU - Slot, L.M. AU - Cavallari, M. AU - Wortel, C.M. AU - Vergroesen, R.D. AU - Bonger, K.M. AU - Reth, M. AU - Toes, R.E.M. PY - 2020 UR - https://hdl.handle.net/2066/218818 TI - Antibodies and B cells recognising citrullinated proteins display a broad cross-reactivity towards other post-translational modifications EP - 480 SN - 0003-4967 IS - iss. 4 SP - 472 JF - Annals of the Rheumatic Diseases VL - vol. 79 DO - https://doi.org/10.1136/annrheumdis-2019-216499 ER - TY - JOUR AU - Bright, A.R. AU - Genesen, S.T. van AU - Li, Q. AU - Heeringen, S.J. van AU - Grasso, A. AU - Veenstra, G.J.C. PY - 2020 UR - https://hdl.handle.net/2066/226891 TI - Combinatorial action of transcription factors in open chromatin contributes to early cellular heterogeneity and organizer mesendoderm specification JF - Biorxiv DO - https://doi.org/10.1101/2020.02.26.966168v1 ER - TY - JOUR AU - Bont, C.M. de AU - Stokman, M. AU - Faas, Priscilla AU - Thurlings, R.M. AU - Boelens, W.C. AU - Wright, Helen L. AU - Pruijn, G.J.M. PY - 2020 UR - https://hdl.handle.net/2066/222128 TI - Autoantibodies to neutrophil extracellular traps represent a potential serological biomarker in rheumatoid arthritis SN - 0896-8411 JF - Journal of Autoimmunity VL - vol. 113 DO - https://doi.org/10.1016/j.jaut.2020.102484 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/222128/222128.pdf?sequence=1 ER - TY - JOUR AU - Kleerekooper, Iris AU - Herbert, M.K. AU - Kuiperij, B. AU - Sato, D. AU - Fujihara, Kazuo AU - Callegaro, Dagoberto AU - Verbeek, M.M. AU - Petzold, A. PY - 2020 UR - https://hdl.handle.net/2066/220046 TI - CSF levels of glutamine synthetase and GFAP to explore astrocytic damage in seronegative NMOSD EP - 611 SN - 0022-3050 IS - iss. 6 SP - 605 JF - Journal of Neurology, Neurosurgery, and Psychiatry VL - vol. 91 DO - https://doi.org/10.1136/jnnp-2019-322286 ER - TY - JOUR AU - Kappel, R. AU - Pruijn, G. PY - 2020 UR - https://hdl.handle.net/2066/226658 TI - Explantation of Silicone Breast Implants Ameliorates Gel Bleed Related Health Complaints in Women with Breast Implant Illness EP - 7 SN - 2378-3656 IS - iss. 3 SP - 1 JF - Clinical Medical Reviews and Case Reports VL - vol. 7 DO - https://doi.org/10.23937/2378-3656/1410301 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/226658/226658.pdf?sequence=1 ER - TY - JOUR AU - Ohto-Fujita, E. AU - Hayasaki, S. AU - Atomi, A. AU - Fujiki, S. AU - Watanabe, T. AU - Boelens, W.C. AU - Shimizu, M. AU - Atomi, Y. PY - 2020 UR - https://hdl.handle.net/2066/227645 TI - Dynamic localization of alpha B-crystallin at the microtubule cytoskeleton network in beating heart cells EP - 137 SN - 0021-924X IS - iss. 2 SP - 125 JF - Journal of Biochemistry VL - vol. 168 DO - https://doi.org/10.1093/jb/mvaa025 ER - TY - JOUR AU - Moorlag, S.J.C.F.M. AU - Rodriguez Rosales, Y.A. AU - Gillard, J.J. AU - Fanucchi, Stephanie AU - Theunissen, K. AU - Novakovic, Boris AU - Bont, C.M. de AU - Fok, E.T. AU - Mourits, V.P. AU - Koeken, V.A.C.M. AU - Bree, L.C.J. de AU - Pruijn, G.J.M. AU - Crevel, R. van AU - Joosten, L.A.B. AU - Joosten, I. AU - Mhlanga, M.M.K. AU - Diavatopoulos, D.A. AU - Chavakis, T. AU - Netea, M.G. PY - 2020 UR - https://hdl.handle.net/2066/227444 TI - BCG Vaccination Induces Long-Term Functional Reprogramming of Human Neutrophils SN - 2211-1247 IS - iss. 7 JF - Cell Reports VL - vol. 33 DO - https://doi.org/10.1016/j.celrep.2020.108387 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/227444/227444.pdf?sequence=1 ER - TY - JOUR AU - Pieters, B.J. AU - Wuts, M.H.M. AU - Poater, Jordi AU - Kumar, Kiran AU - White, P. AU - Kamps, J. AU - Pruijn, G. AU - Matthias Bickelhaupt, F. AU - Mecinovic, J. PY - 2020 UR - https://hdl.handle.net/2066/220109 TI - Mechanism of biomolecular recognition of trimethyllysine by the fluorinated aromatic cage of KDM5A PHD3 finger SN - 2399-3669 IS - iss. 1 JF - Communications Chemistry VL - vol. 3 DO - https://doi.org/10.1038/s42004-020-0313-2 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/220109/220109.pdf?sequence=1 ER - TY - JOUR AU - Uhd, Jesper AU - Miotke, Laura AU - Ji, Hanlee P. AU - Dunaeva, Marina AU - Pruijn, G. AU - Jorgensen, Christian AU - Hansen, J. AU - Astakhova, Kira PY - 2020 UR - https://hdl.handle.net/2066/225089 TI - Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay EP - 5844 SN - 0003-2654 IS - iss. 17 SP - 5836 JF - Analyst VL - vol. 145 DO - https://doi.org/10.1039/d0an00676a ER - TY - JOUR AU - Boelens, Wilbert C. PY - 2020 UR - https://hdl.handle.net/2066/219179 TI - Structural aspects of the human small heat shock proteins related to their functional activities SN - 1355-8145 JF - Cell Stress & Chaperones DO - https://doi.org/10.1007/s12192-020-01093-1 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/219179/219179.pdf?sequence=1 ER - TY - JOUR AU - Lelieveldt, L.P.W.M. AU - Eising, S. AU - Wijen, Abel AU - Bonger, Kimberly M. PY - 2019 UR - https://hdl.handle.net/2066/208944 TI - Vinylboronic acid-caged prodrug activation using click-to-release tetrazine ligation EP - 8821 SN - 1477-0520 IS - iss. 39 SP - 8816 JF - Organic & Biomolecular Chemistry VL - vol. 17 DO - https://doi.org/10.1039/c9ob01881f L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/208944/208944.pdf?sequence=1 ER - TY - JOUR AU - Rietveld, A. AU - Lassche, S. AU - Engelen, B.G.M. van AU - Kusters, B. AU - Saris, C.G.J. AU - Pruijn, G.J.M. PY - 2019 UR - https://hdl.handle.net/2066/214820 TI - Nonspecific pattern of muscular cN-1A expression in inclusion body myositis SN - 2520-1026 IS - iss. 1 JF - BMC Rheumatology VL - vol. 3 DO - https://doi.org/10.1186/s41927-019-0078-3 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/214820/214820.pdf?sequence=1 ER - TY - JOUR AU - Bont, C.M. de AU - Eerden, N. AU - Boelens, W.C. AU - Pruijn, G. PY - 2019 UR - https://hdl.handle.net/2066/212264 TI - Neutrophil proteases degrade autoepitopes of NET-associated proteins EP - 8 SN - 0009-9104 SP - 1 JF - Clinical and Experimental Immunology VL - vol. 199 DO - https://doi.org/10.1111/cei.13392 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/212264/212264.pdf?sequence=1 ER - TY - JOUR AU - Maio, Antonio De AU - Cauvi, David M. AU - Capone, Ricardo AU - Bello, Ivan AU - Vree Egberts, W.T.M. AU - Arispe, Nelson AU - Boelens, Wilbert PY - 2019 UR - https://hdl.handle.net/2066/207681 TI - The small heat shock proteins, HSPB1 and HSPB5, interact differently with lipid membranes EP - 956 SN - 1355-8145 IS - iss. 5 SP - 947 JF - Cell Stress & Chaperones VL - vol. 24 DO - https://doi.org/10.1007/s12192-019-01021-y ER - TY - JOUR AU - Bont, C.M. de AU - Boelens, W.C. AU - Pruijn, G.J.M. PY - 2019 UR - https://hdl.handle.net/2066/214837 TI - Proteolytic modification of NET components affects their recognition by autoantibodies EP - 64 SN - 0014-2972 IS - iss. 1 SP - 63 JF - European Journal of Clinical Investigation VL - vol. 49 DO - https://doi.org/10.1111/eci.13108 ER - TY - JOUR AU - Berden, Giel AU - Derksen, M. AU - Houthuijs, Kas J. AU - Martens, Jonathan AU - Oomens, J. PY - 2019 UR - https://hdl.handle.net/2066/206038 TI - An automatic variable laser attenuator for IRMPD spectroscopy and analysis of power-dependence in fragmentation spectra EP - 8 SN - 1387-3806 SP - 1 JF - International Journal of Mass Spectrometry VL - vol. 443 DO - https://doi.org/10.1016/j.ijms.2019.05.013 ER - TY - JOUR AU - Bode, S.A. AU - Timmermans, S.B.P.E. AU - Eising, S. AU - Gemert, S.P.W. van AU - Bonger, K.M. AU - Lowik, D.W.P.M. PY - 2019 UR - https://hdl.handle.net/2066/201113 TI - Click to enter: activation of oligo-arginine cell-penetrating peptides by bioorthogonal tetrazine ligations EP - 705 SN - 2041-6520 IS - iss. 3 SP - 701 JF - Chemical Science VL - vol. 10 DO - https://doi.org/10.1039/c8sc04394a L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/201113/201113.pdf?sequence=1 ER - TY - JOUR AU - de Bont, Cynthia M. AU - Boelens, W.C. AU - Pruijn, G.J.M. PY - 2019 UR - https://hdl.handle.net/2066/214819 TI - NETosis, complement, and coagulation: a triangular relationship EP - 27 SN - 1672-7681 IS - iss. 1 SP - 19 JF - Cellular & Molecular Immunology VL - vol. 16 DO - https://doi.org/10.1038/s41423-018-0024-0 ER - TY - JOUR AU - Rietveld, A. AU - Lim, J. AU - Visser, M de AU - Engelen, B.G.M. van AU - Pruijn, G. AU - Benveniste, O. AU - Kooi, A. van der AU - Saris, C.G.J. PY - 2019 UR - https://hdl.handle.net/2066/207009 AB - The diagnosis and classification of idiopathic inflammatory myopathies are based mainly on clinical and histological features. The discovery of myositis-specific and myositis-associated antibodies has simplified the (sub)classification of inflammatory myopathies. Patients suspected of having an idiopathic inflammatory myopathy should undergo routine antibody testing to gain more insight into distinct phenotypes, comorbidities, treatment response and prognosis. Furthermore, autoantibody testing can help in patients with atypical patterns of weakness or with an unresolved limb-girdle myopathic phenotype, or interstitial lung disease. However, some important technical and methodological issues can hamper the interpretation of antibody testing; for example, some antibodies are not included in the widely available line blots. We aim to provide a practical review of the use of autoantibody testing in idiopathic inflammatory myopathies in clinical practice. TI - Autoantibody testing in idiopathic inflammatory myopathies EP - 294 SN - 1474-7758 IS - iss. 4 SP - 284 JF - Practical Neurology VL - vol. 19 DO - https://doi.org/10.1136/practneurol-2017-001742 ER - TY - JOUR AU - Eising, S. AU - Linden, Nicole G.A. van der AU - Kleinpenning, F. AU - Bonger, K.M. PY - 2018 UR - https://hdl.handle.net/2066/191214 TI - Vinylboronic Acids as Efficient Bioorthogonal Reactants for Tetrazine Labeling in Living Cells EP - 986 SN - 1043-1802 IS - iss. 4 SP - 982 JF - Bioconjugate Chemistry VL - vol. 29 DO - https://doi.org/10.1021/acs.bioconjchem.7b00796 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/191214/191214.pdf?sequence=1 ER - TY - JOUR AU - Gracht, Anouk M.F. van der AU - Geus, Mark A.R. de AU - Camps, Marcel G.M. AU - Ruckwardt, Tracy J. AU - Sarris, Alexi J.C. AU - Bremmers, Jessica AU - Bonger, K.M. AU - Ossendorp, Ferry AU - Kasteren, Sander, I van PY - 2018 UR - https://hdl.handle.net/2066/193094 TI - Chemical Control over T-Cell Activation in Vivo Using Deprotection of trans-Cyclooctene-Modified Epitopes EP - 1576 SN - 1554-8929 IS - iss. 6 SP - 1569 JF - Acs Chemical Biology VL - vol. 13 DO - https://doi.org/10.1021/acschembio.8b00155 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/193094/193094.pdf?sequence=1 ER - TY - JOUR AU - Clark, Alice R. AU - Vree Egberts, W.T.M. AU - Kondrat, Frances D.L. AU - Hilton, Gillian R. AU - Ray, Nicholas J. AU - Cole, Ambrose R. AU - Boelens, Wilbert C. AU - Slingsby, C PY - 2018 UR - https://hdl.handle.net/2066/195544 TI - Terminal Regions Confer Plasticity to the Tetrameric Assembly of Human HspB2 and HspB3 EP - 3310 SN - 0022-2836 IS - iss. 18 SP - 3297 JF - Journal of Molecular Biology VL - vol. 430 DO - https://doi.org/10.1016/j.jmb.2018.06.047 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/195544/195544.pdf?sequence=1 ER - TY - JOUR AU - Schoonen, Lise AU - Eising, S. AU - Eldijk, M.B. van AU - Bresseleers, Jaleesa AU - Pijl, Margo van der AU - Nolte, R.J.M. AU - Bonger, K.M. AU - Hest, J. van PY - 2018 UR - https://hdl.handle.net/2066/191177 TI - Modular, Bioorthogonal Strategy for the Controlled Loading of Cargo into a Protein Nanocage EP - 1193 SN - 1043-1802 IS - iss. 4 SP - 1186 JF - Bioconjugate Chemistry VL - vol. 29 DO - https://doi.org/10.1021/acs.bioconjchem.7b00815 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/191177/191177.pdf?sequence=1 ER - TY - JOUR AU - Eising, S. AU - Engwerda, A.H.J. AU - Riedijk, Xian AU - Bickelhaupt, F.Matthias AU - Bonger, K.M. PY - 2018 UR - https://hdl.handle.net/2066/196225 TI - Highly Stable and Selective Tetrazines for the Coordination-Assisted Bioorthogonal Ligation with Vinylboronic Acids EP - 3059 SN - 1043-1802 IS - iss. 9 SP - 3054 JF - Bioconjugate Chemistry VL - vol. 29 DO - https://doi.org/10.1021/acs.bioconjchem.8b00439 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/196225/196225.pdf?sequence=1 ER - TY - JOUR AU - Perino, M. AU - Mierlo, G. van AU - Karemaker, I.D. AU - Genesen, Siebe van AU - Vermeulen, Michiel AU - Marks, Hendrik AU - Heeringen, S.J. van AU - Veenstra, Gert Jan C. PY - 2018 UR - https://hdl.handle.net/2066/194082 TI - MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding EP - 1016 SN - 1061-4036 IS - iss. 7 SP - 1002 JF - Nature Genetics VL - vol. 50 DO - https://doi.org/10.1038/s41588-018-0134-8 ER - TY - JOUR AU - Kleinpenning, F. AU - Eising, S. AU - Berkenbosch, Tim AU - Garzero, Veronica AU - Schaart, Judith M. AU - Bonger, K.M. PY - 2018 UR - https://hdl.handle.net/2066/214648 TI - Correction to Subcellular Protein Labeling by a Spatially Restricted Arylamine N-Acetyltransferase EP - 313 SN - 1554-8929 IS - iss. 2 SP - 313 JF - Acs Chemical Biology VL - vol. 14 DO - https://doi.org/10.1021/acschembio.8b01091 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/214648/214648.pdf?sequence=1 ER - TY - JOUR AU - Kleinpenning, F. AU - Eising, S. AU - Berkenbosch, Tim AU - Garzero, Veronica AU - Schaart, Judith M. AU - Bonger, K.M. PY - 2018 UR - https://hdl.handle.net/2066/195304 TI - Subcellular Protein Labeling by a Spatially Restricted Arylamine N-Acetyltransferase EP - 1937 SN - 1554-8929 IS - iss. 8 SP - 1932 JF - Acs Chemical Biology VL - vol. 13 DO - https://doi.org/10.1021/acschembio.8b00178 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/195304/195304.pdf?sequence=1 ER - TY - JOUR AU - Lelieveldt, L.P.W.M. AU - Kristyanto, Hendy AU - Pruijn, G. AU - Scherer, H.U. AU - Toes, R.E.M. AU - Bonger, K.M. PY - 2018 UR - https://hdl.handle.net/2066/199106 TI - Sequential Prodrug Strategy To Target and Eliminate ACPA-Selective Autoreactive B Cells EP - 5573 SN - 1543-8384 IS - iss. 12 SP - 5565 JF - Molecular Pharmaceutics VL - vol. 15 DO - https://doi.org/10.1021/acs.molpharmaceut.8b00741 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/199106/199106.pdf?sequence=1 ER - TY - JOUR AU - Polachek, A. AU - Vree Egberts, W.T.M. AU - Fireman, E. AU - Druckman, Ido AU - Stark, M.A. AU - Paran, D. AU - Pruijn, G. AU - Elkayam, O. PY - 2018 UR - https://hdl.handle.net/2066/191472 TI - Sputum Anticitrullinated Protein Antibodies in Patients With Long-standing Rheumatoid Arthritis EP - 126 SN - 1076-1608 IS - iss. 3 SP - 122 JF - Journal of Clinical Rheumatology VL - vol. 24 DO - https://doi.org/10.1097/RHU.0000000000000619 ER - TY - JOUR AU - Embaby, Ahmed M. AU - Lelieveldt, L.P.W.M. AU - Diness, Frederik AU - Meldal, Morten PY - 2018 UR - https://hdl.handle.net/2066/199095 TI - Metallo-Organozymes with Specific Proteolytic Activity EP - 17428 SN - 0947-6539 IS - iss. 66 SP - 17424 JF - Chemistry : a European Journal VL - vol. 24 DO - https://doi.org/10.1002/chem.201803666 ER - TY - JOUR AU - Eising, S. AU - Xin, Bo-Tao AU - Kleinpenning, F. AU - Heming, Jurriaan J.A. AU - Florea, Bogdan I. AU - Overkleeft, Herman S. AU - Bonger, K.M. PY - 2018 UR - https://hdl.handle.net/2066/194248 TI - Coordination-Assisted Bioorthogonal Chemistry: Orthogonal Tetrazine Ligation with Vinylboronic Acid and a Strained Alkene EP - 1652 SN - 1439-4227 IS - iss. 15 SP - 1648 JF - Chembiochem. VL - vol. 19 DO - https://doi.org/10.1002/cbic.201800275 ER - TY - JOUR AU - Bont, C.M. de AU - Koopman, W.J.H. AU - Boelens, W.C. AU - Pruijn, G. PY - 2018 UR - https://hdl.handle.net/2066/196146 TI - Stimulus-dependent chromatin dynamics, citrullination, calcium signaling and ROS production during NET formation. EP - 1629 SN - 0167-4889 SP - 1621 JF - Biochimica et Biophysica Acta. Molecular Cell Research VL - vol. 2018:1865 DO - https://doi.org/10.1016/j.bbamcr.2018.08.014 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/196146/196146.pdf?sequence=1 ER - TY - JOUR AU - Friederich, Marisa W. AU - Timal, S. AU - Powell, Christopher A. AU - Dallabona, Cristina AU - Kurolap, Alina AU - Palacios-Zambrano, Sara AU - Lefeber, D.J. AU - Pruijn, G. AU - Smeitink, J.A.M. AU - Veltman, J.A. AU - Rodenburg, R.J.T. AU - Hove, Johan L.K. Van PY - 2018 UR - https://hdl.handle.net/2066/196650 TI - Pathogenic variants in glutamyl-tRNA(Gln) amidotransferase subunits cause a lethal mitochondrial cardiomyopathy disorder SN - 2041-1723 JF - Nature Communications VL - vol. 9 PS - 14 p. DO - https://doi.org/10.1038/s41467-018-06250-w L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/196650/196650.pdf?sequence=1 ER - TY - JOUR AU - Dunaeva, M. AU - Blom, J. AU - Thurlings, R.M. AU - Pruijn, G.J.M. PY - 2018 UR - https://hdl.handle.net/2066/195841 TI - Circulating serum miR-223-3p and miR-16-5p as possible biomarkers of early rheumatoid arthritis EP - 385 SN - 0009-9104 IS - iss. 3 SP - 376 JF - Clinical and Experimental Immunology VL - vol. 193 DO - https://doi.org/10.1111/cei.13156 ER - TY - JOUR AU - Rietveld, A. AU - Hoogen, L.L. van den AU - Bizzaro, Nicola AU - Blokland, Sofie L.M. AU - Daehnrich, Cornelia AU - Gottenberg, J.-E. AU - Engelen, B.G.M. van AU - Saris, C.G.J. AU - Pruijn, G. PY - 2018 UR - https://hdl.handle.net/2066/192050 TI - Autoantibodies to Cytosolic 5 '-Nucleotidase 1A in Primary Sjogren's Syndrome and Systemic Lupus Erythematosus SN - 1664-3224 JF - Frontiers in Immunology VL - vol. 9 DO - https://doi.org/10.3389/fimmu.2018.01200 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/192050/192050.pdf?sequence=1 ER - TY - JOUR AU - Carra, S. AU - Alberti, S. AU - Arrigo, P.A. AU - Benesch, J.L. AU - Benjamin, I.J. AU - Boelens, W.C. AU - Bartelt-Kirbach, B. AU - Brundel, B. AU - Buchner, J. AU - Bukau, B. AU - Carver, J.A. AU - Ecroyd, H. AU - Emanuelsson, C. AU - Finet, S. AU - Golenhofen, N. AU - Goloubinoff, P. AU - Gusev, N. AU - Haslbeck, M. AU - Hightower, L.E. AU - Kampinga, H.H. AU - Klevit, R.E. AU - Liberek, K. AU - McHaourab, H.S. AU - McMenimen, K.A. AU - Poletti, A. AU - Quinlan, R. AU - Strelkov, S.V. AU - Toth, M.E. AU - Vierling, E. AU - Tanguay, R.M. PY - 2017 UR - https://hdl.handle.net/2066/174455 TI - The growing world of small heat shock proteins: From structure to functions EP - 611 SN - 1355-8145 IS - iss. 4 SP - 601 JF - Cell Stress & Chaperones VL - vol. 22 DO - http://dx.doi.org/10.1007/s12192-017-0787-8 ER - TY - JOUR AU - Bloemers, H.P.J. AU - Boelens, W.C. PY - 2017 UR - https://hdl.handle.net/2066/174422 TI - In memoriam Wilfried de Jong (1942-2016) EP - 454 SN - 1355-8145 IS - iss. 4 SP - 453 JF - Cell Stress & Chaperones VL - vol. 22 DO - http://dx.doi.org/10.1007/s12192-016-0738-9 ER - TY - JOUR AU - Bawadekar, M. AU - Shim, D. AU - Johnson, C.J. AU - Warner, T.F. AU - Rebernick, R. AU - Damgaard, D. AU - Nielsen, C.H. AU - Pruijn, G.J.M. AU - Nett, J.E. AU - Shelef, M.A. PY - 2017 UR - https://hdl.handle.net/2066/169078 TI - Peptidylarginine deiminase 2 is required for tumor necrosis factor alpha-induced citrullination and arthritis, but not neutrophil extracellular trap formation EP - 47 SN - 0896-8411 SP - 39 JF - Journal of Autoimmunity VL - vol. 80 DO - http://dx.doi.org/10.1016/j.jaut.2017.01.006 ER - TY - JOUR AU - Steinbusch, M.M.F. AU - Caron, M.M.J. AU - Surtel, D.A.M. AU - Friedrich, F. AU - Lausch, E. AU - Pruijn, G. AU - Verhesen, W. AU - Schroen, B.L.M. AU - van Rhijn, L.W. AU - Zabel, B. AU - Welting, T.J.M. PY - 2017 UR - https://hdl.handle.net/2066/177137 TI - Expression of RMRP RNA is regulated in chondrocyte hypertrophy and determines chondrogenic differentiation EP - 15 SN - 2045-2322 SP - 1 JF - Scientific Reports VL - vol. 7 PS - 15 p. DO - https://doi.org/10.1038/s41598-017-06809-5 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/177137/177137.pdf?sequence=1 ER - TY - JOUR AU - Dunaeva, M. AU - Waltenberger, J. PY - 2017 UR - https://hdl.handle.net/2066/177141 TI - Hh signaling in regeneration of the ischemic heart EP - 3490 SN - 1420-682X IS - iss. 19 SP - 3481 JF - Cellular and Molecular Life Sciences VL - vol. 74 DO - http://dx.doi.org/10.1007/s00018-017-2534-9 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/177141/177141.pdf?sequence=1 ER - TY - JOUR AU - Bode, S.A. AU - Kruis, I.C. AU - Adams, P.J.H.M. AU - Boelens, W.C. AU - Pruijn, G.J.M. AU - Hest, J.C.M. van AU - Lowik, D.W.P.M. PY - 2017 UR - https://hdl.handle.net/2066/166194 TI - Coiled-Coil-Mediated Activation of Oligoarginine Cell-Penetrating Peptides EP - 188 SN - 1439-4227 IS - iss. 2 SP - 185 JF - Chembiochem. VL - vol. 18 DO - https://doi.org/10.1002/cbic.201600614 ER - TY - JOUR AU - Dunaeva, M. AU - Derksen, M. AU - Pruijn, G.J.M. PY - 2017 UR - https://hdl.handle.net/2066/177153 TI - LINE-1 Hypermethylation in serum cell-free DNA of relapsing remitting multiple sclerosis patients EP - 8 SN - 0893-7648 SP - 1 JF - Molecular Neurobiology PS - 8 p. DO - https://doi.org/10.1007/s12035-017-0679-z L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/177153/177153.pdf?sequence=1 ER - TY - JOUR AU - Rothwell, S. AU - Cooper, R.G. AU - Lundberg, I.E. AU - Gregersen, P.K. AU - Hanna, M.G. AU - Machado, P.M. AU - Herbert, M.K. AU - Pruijn, G.J.M. AU - Lilleker, J.B. AU - Roberts, M AU - Bowes, J. AU - Seldin, M.F. AU - Vencovsky, J. AU - Danko, K. AU - Limaye, V. AU - Selva-O'Callaghan, A. AU - Platt, H. AU - Molberg, O. AU - Benveniste, O. AU - Radstake, T.R. AU - Doria, A. AU - De Bleecker, J. AU - De Paepe, B. AU - Gieger, C. AU - Meitinger, T. AU - Winkelmann, J. AU - Amos, C.I. AU - Ollier, W.E. AU - Padyukov, L. AU - Lee, A.T van der AU - Lamb, J.A. AU - Chinoy, H. PY - 2017 UR - https://hdl.handle.net/2066/169069 TI - Immune-Array Analysis in Sporadic Inclusion Body Myositis Reveals HLA-DRB1 Amino Acid Heterogeneity Across the Myositis Spectrum EP - 1099 SN - 2326-5191 IS - iss. 5 SP - 1090 JF - Arthritis & Rheumatology VL - vol. 69 PS - 10 p. DO - https://doi.org/10.1002/art.40045 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/169069/169069.pdf?sequence=1 ER - TY - JOUR AU - Wortmann, S.B. AU - Timal, S. AU - Venselaar, H. AU - Wintjes, L.T. AU - Kopajtich, R. AU - Feichtinger, R.G. AU - Onnekink, C. AU - Mühlmeister, M. AU - Brandt, U. AU - Smeitink, J.A.M. AU - Veltman, J.A. AU - Sperl, W. AU - Lefeber, D.J. AU - Pruijn, G. AU - Stojanovic, V. AU - Freisinger, P. AU - Spronsen, F. van AU - Derks, T.G. AU - Veenstra-Knol, H.E. AU - Mayr, J.A. AU - Rötig, A. AU - Tarnopolsky, M. AU - Prokisch, H. AU - Rodenburg, R.J.T. PY - 2017 UR - https://hdl.handle.net/2066/182439 TI - Biallelic variants in WARS2 encoding mitochondrial tryptophanyl-tRNA synthase in six individuals with mitochondrial encephalopathy EP - 1795 SN - 1059-7794 IS - iss. 12 SP - 1786 JF - Human Mutation VL - vol. 38 DO - https://doi.org/10.1002/humu.23340 ER - TY - JOUR AU - Lilleker, J.B. AU - Rietveld, A. AU - Pye, S.R. AU - Mariampillai, K. AU - Benveniste, O. AU - Peeters, M.T.J. AU - Miller, J.A.L. AU - Hanna, M.G. AU - Machado, P.M. AU - Parton, M.J. AU - Gheorghe, K.R. AU - Badrising, U.A. AU - Lundberg, I.E. AU - Sacconi, S. AU - Herbert, M.K. AU - McHugh, N.J. AU - Lecky, B.R.F. AU - Brierley, C. AU - Hilton-Jones, D. AU - Lamb, J.A. AU - Roberts, M.E. AU - Cooper, R.G. AU - Saris, C.G.J. AU - Pruijn, G. AU - Chinoy, H. AU - Engelen, B.G.M. van PY - 2017 UR - https://hdl.handle.net/2066/174409 TI - Cytosolic 5 '-nucleotidase 1A autoantibody profile and clinical characteristics in inclusion body myositis EP - 868 SN - 0003-4967 IS - iss. 5 SP - 862 JF - Annals of the Rheumatic Diseases VL - vol. 76 DO - https://doi.org/10.1136/annrheumdis-2016-210453 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/174409/174409.pdf?sequence=1 ER - TY - JOUR AU - Conrad, K. AU - Andrade, L.E.C. AU - Chan, E.K.L. AU - Mahler, M. AU - Meroni, P.L. AU - Pruijn, G.J.M. AU - Steiner, G. AU - Shoenfeld, Y. PY - 2016 UR - https://hdl.handle.net/2066/159975 TI - From autoantibody research to standardized diagnostic assays in the management of human diseases - report of the 12th Dresden Symposium on Autoantibodies EP - 796 SN - 0961-2033 IS - iss. 8 SP - 787 JF - Lupus VL - vol. 25 DO - http://dx.doi.org/10.1177/0961203316644337 ER - TY - JOUR AU - Damgaard, D. AU - Bjorn, M.E. AU - Steffensen, M.A. AU - Pruijn, G.J.M. AU - Nielsen, C.H. PY - 2016 UR - https://hdl.handle.net/2066/158780 TI - Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase EP - 14 SN - 1478-6354 SP - 7 JF - Arthritis Research & Therapy VL - vol. 18 DO - http://dx.doi.org/10.1186/s13075-016-1000-7 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/158780/158780.pdf?sequence=1 ER - TY - JOUR AU - Jakobsson, P.J. AU - Fernandes-Cerqueira, C. AU - Sohrabian, A. AU - Albrecht, I. AU - Notarnicola, A. AU - Ossipova, E. AU - Lengqvist, J. AU - Fathi, M. AU - Pruijn, G.J.M. AU - Grunewald, J. AU - Ronnelid, J. AU - Lundberg, I.E. PY - 2016 UR - https://hdl.handle.net/2066/161490 TI - Characterization of extracellular histidyl-tRNA synthetase in myositis EP - 8 SN - 0300-9742 SP - 8 JF - Scandinavian Journal of Rheumatology VL - vol. 45 DO - http://dx.doi.org/10.1136/annrheumdis-2016-eular.5670 ER - TY - JOUR AU - Kramp, S.L. AU - Karayev, D. AU - Shen, G. AU - Metzger, A.L. AU - Morris, R.I. AU - Karayev, E. AU - Lam, Y. AU - Kazdan, R.M. AU - Pruijn, G. AU - Saschenbrecker, S. AU - Dähnrich, C. AU - Schlumberger, W. PY - 2016 UR - https://hdl.handle.net/2066/166205 TI - Development and evaluation of a standardized ELISA for the determination of autoantibodies against cN-1A (Mup44, NT5C1A) in sporadic inclusion body myositis EP - 32 SN - 2038-3274 IS - iss. 1 SP - 16 JF - Autoimmunity Highlights VL - vol. 7 PS - 16 p. DO - http://dx.doi.org/10.1007/s13317-016-0088-8 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/166205/166205.pdf?sequence=1 ER - TY - JOUR AU - Rombouts, Y. AU - Willemze, A. AU - Beers, J.J.B.C. van AU - Shi, J. AU - Kerkman, P.F. AU - van Toorn, L. AU - Janssen, G.M.C. AU - Zaldumbide, A. AU - Hoeben, R.C. AU - Pruijn, G. AU - Deelder, A.M. AU - Wolbink, G. AU - Rispens, T. AU - van Veelen, P.A. AU - Huizinga, T.W.J. AU - Wuhrer, M. AU - Trouw, L.A. AU - Scherer, H.U. AU - Toes, R.E.M. PY - 2016 UR - https://hdl.handle.net/2066/166218 TI - Extensive glycosylation of ACPA-IgG variable domains modulates binding to citrullinated antigens in rheumatoid arthritis EP - 585 SN - 0003-4967 IS - iss. 3 SP - 578 JF - Annals of the Rheumatic Diseases VL - vol. 75 DO - http://dx.doi.org/10.1136/annrheumdis-2014-206598 ER - TY - JOUR AU - Lilleker, J. AU - Rietveld, A AU - Pye, S. AU - Lamb, J. AU - Roberts, M AU - Cooper, R. AU - Pruijn, G. AU - Chinoy, H. AU - van Engelen, B. PY - 2016 UR - https://hdl.handle.net/2066/166230 TI - Implications of Anti-Cn1a Serotype in Inclusion Body Myositis EP - 2 SN - 0022-3050 IS - iss. 12 SP - 1 JF - Journal of Neurology, Neurosurgery, and Psychiatry VL - vol. 87 PS - 1 p. DO - http://dx.doi.org/10.1136/jnnp-2016-315106.27 ER - TY - JOUR AU - Joshua, V. AU - Schobers, L. AU - Titcombe, P.J. AU - Israelsson, L. AU - Ronnelid, J. AU - Hansson, M. AU - Catrina, A.I. AU - Pruijn, G. AU - Malmstrom, V. PY - 2016 UR - https://hdl.handle.net/2066/166266 TI - Antibody responses to de novo identified citrullinated fibrinogen peptides in rheumatoid arthritis and visualization of the corresponding B cells EP - 18 SN - 1478-6354 SP - 9 JF - Arthritis Research & Therapy VL - vol. 18 PS - 9 p. DO - http://dx.doi.org/10.1186/s13075-016-1181-0 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/166266/166266.pdf?sequence=1 ER - TY - JOUR AU - Eising, S. AU - Lelivelt, F. AU - Bonger, K.M. PY - 2016 UR - https://hdl.handle.net/2066/163349 TI - Vinylboronic Acids as Fast Reacting, Synthetically Accessible, and Stable Bioorthogonal Reactants in the Carboni-Lindsey Reaction EP - 12247 SN - 1433-7851 IS - iss. 40 SP - 12243 JF - Angewandte Chemie. International Edition VL - vol. 55 DO - http://dx.doi.org/10.1002/anie.201605271 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/163349/163349.pdf?sequence=1 ER - TY - JOUR AU - Kruis, I.C. AU - Lowik, D. AU - Boelens, W.C. AU - van Hest, J.C.M. AU - Pruijn, G. PY - 2016 UR - https://hdl.handle.net/2066/161532 TI - An integrated, peptide-based approach to site-specific protein immobilization for detection of biomolecular interactions EP - 5328 SN - 0003-2654 IS - iss. 18 SP - 5321 JF - Analyst VL - vol. 141 DO - https://doi.org/10.1039/c6an00154h L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/161532/161532pos.pdf?sequence=1 ER - TY - JOUR AU - Herbert, M.K. AU - Stammen-Vogelzangs, J. AU - Verbeek, M.M. AU - Rietveld, A. AU - Lundberg, I.E. AU - Chinoy, H. AU - Lamb, J.A. AU - Cooper, R.G. AU - Roberts, M.J. AU - Badrising, U.A. AU - Bleecker, J.L. De AU - Machado, P.M. AU - Hanna, M.G. AU - Plestilova, L. AU - Vencovsky, J. AU - Engelen, B.G.M. van AU - Pruijn, G.J.M. PY - 2016 UR - https://hdl.handle.net/2066/166344 AB - OBJECTIVES: The diagnosis of inclusion body myositis (IBM) can be challenging as it can be difficult to clinically distinguish from other forms of myositis, particularly polymyositis (PM). Recent studies have shown frequent presence of autoantibodies directed against cytosolic 5'-nucleotidase 1A (cN-1A) in patients with IBM. We therefore, examined the autoantigenicity and disease specificity of major epitopes of cN-1A in patients with sporadic IBM compared with healthy and disease controls. METHODS: Serum samples obtained from patients with IBM (n=238), PM and dermatomyositis (DM) (n=185), other autoimmune diseases (n=246), other neuromuscular diseases (n=93) and healthy controls (n=35) were analysed for the presence of autoantibodies using immunodominant cN-1A peptide ELISAs. RESULTS: Autoantibodies directed against major epitopes of cN-1A were frequent in patients with IBM (37%) but not in PM, DM or non-autoimmune neuromuscular diseases (<5%). Anti-cN-1A reactivity was also observed in some other autoimmune diseases, particularly Sjogren's syndrome (SjS; 36%) and systemic lupus erythematosus (SLE; 20%). CONCLUSIONS: In summary, we found frequent anti-cN-1A autoantibodies in sera from patients with IBM. Heterogeneity in reactivity with the three immunodominant epitopes indicates that serological assays should not be limited to a distinct epitope region. The similar reactivities observed for SjS and SLE demonstrate the need to further investigate whether distinct IBM-specific epitopes exist. TI - Disease specificity of autoantibodies to cytosolic 5'-nucleotidase 1A in sporadic inclusion body myositis versus known autoimmune diseases EP - 701 SN - 0003-4967 IS - iss. 4 SP - 696 JF - Annals of the Rheumatic Diseases VL - vol. 75 DO - https://doi.org/10.1136/annrheumdis-2014-206691 ER - TY - JOUR AU - Buggenum, A.G.L. van AU - Gerlach, J.P. AU - Eising, S. AU - Schoonen, L. AU - Eijl, R.A.P.M. van AU - Tanis, E.J. AU - Hogeweg, M. AU - Hubner, N.C. AU - van Hest, J.C.M. AU - Bonger, K.M. AU - Mulder, K.W. PY - 2016 UR - https://hdl.handle.net/2066/156910 TI - A covalent and cleavable antibody-DNA conjugation strategy for sensitive protein detection via immuno-PCR EP - 12 SN - 2045-2322 SP - 1 JF - Scientific Reports VL - vol. 6 DO - https://doi.org/10.1038/srep22675 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/156910/156910.pdf?sequence=1 ER - TY - JOUR AU - Maten, E. van der AU - Bont, C.M. de AU - Groot, R. de AU - Jonge, M.I. de AU - Langereis, J.D. AU - Flier, M. van der PY - 2016 UR - https://hdl.handle.net/2066/171105 AB - Bacterial pathogens not only stimulate innate immune receptors, but also activate the complement system. Crosstalk between complement C5a receptor (C5aR) and other innate immune receptors is known to enhance the proinflammatory cytokine response. An important determinant of the magnitude of complement activation is the activity of the alternative pathway, which serves as an amplification mechanism for complement activation. Both alternative pathway activity as well as plasma levels of factor H, a key inhibitor of the alternative pathway, show large variation within the human population. Here, we studied the effect of factor H-mediated regulation of the alternative pathway on bacterial-induced proinflammatory cytokine responses. We used the human pathogen Streptococcus pneumoniae as a model stimulus to induce proinflammatory cytokine responses in human peripheral blood mononuclear cells. Serum containing active complement enhanced pneumococcal induced proinflammatory cytokine production through C5a release and C5aR crosstalk. We found that inhibition of the alternative pathway by factor H, with a concentration equivalent to a high physiological level, strongly reduced C5a levels and decreased proinflammatory cytokine production in human peripheral blood mononuclear cells. This suggests that variation in alternative pathway activity due to variation in factor H plasma levels affects individual cytokine responses during infection. TI - Alternative pathway regulation by factor H modulates Streptococcus pneumoniae induced proinflammatory cytokine responses by decreasing C5a receptor crosstalk EP - 286 SN - 1043-4666 SP - 281 JF - Cytokine VL - vol. 88 DO - https://doi.org/10.1016/j.cyto.2016.09.025 ER - TY - JOUR AU - Pruijn, G.J.M. PY - 2015 UR - https://hdl.handle.net/2066/150338 TI - Citrullination and carbamylation in the pathophysiology of rheumatoid arthritits EP - 5 SN - 1664-3224 SP - 1 JF - Frontiers in Immunology VL - vol. 6 PS - 5 p. DO - http://dx.doi.org/10.3389/fimmu.2015.00192 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/150338/150338.pdf?sequence=1 ER - TY - JOUR AU - Collins, S.R. AU - Yang, H.W. AU - Bonger, K.M. AU - Guignet, E.G. AU - Wandless, T.J. AU - Meyer, T. PY - 2015 UR - https://hdl.handle.net/2066/144898 TI - Using light to shape chemical gradients for parallel and automated analysis of chemotaxis SN - 1744-4292 IS - iss. 4 JF - Molecular Systems Biology VL - vol. 11 DO - http://dx.doi.org/10.15252/msb.20156027 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/144898/144898.pdf?sequence=1 ER - TY - JOUR AU - Hensen, S.M.M. AU - Boelens, W.C. AU - Bonger, K.M. AU - Cruchten, R.T.P. van AU - Delft, F.L. van AU - Pruijn, G.J.M. PY - 2015 UR - https://hdl.handle.net/2066/144977 TI - Phenylglyoxal-Based Visualization of Citrullinated Proteins on Western Blots EP - 6600 SN - 1420-3049 IS - iss. 4 SP - 6592 JF - Molecules VL - vol. 20 DO - http://dx.doi.org/10.3390/molecules20046592 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/144977/144977.pdf?sequence=1 ER - TY - JOUR AU - Dunaeva, M. AU - Buddingh, B. AU - Toes, R.M. AU - Luime, J. AU - Lubberts, E. AU - Pruijn, G.J.M. PY - 2015 UR - https://hdl.handle.net/2066/170166 TI - Decreased serum cell-free DNA levels in rheumatoid arthritis EP - 30 SN - 2038-3274 IS - iss. 1-2 SP - 23 JF - Autoimmunity Highlights VL - vol. 6 DO - http://dx.doi.org/10.1007/s13317-015-0066-6 ER - TY - JOUR AU - Herbert, M.K. AU - Pruijn, G.J.M. PY - 2015 UR - https://hdl.handle.net/2066/150325 TI - Novel serology testing for sporadic inclusion body myositis: disease-specificity and diagnostic utility EP - 600 SN - 1040-8711 IS - iss. 6 SP - 595 JF - Current Opinion in Rheumatology VL - vol. 27 DO - https://doi.org/10.1097/BOR.0000000000000216 ER - TY - JOUR AU - Derksen, M. AU - Mertens, V. AU - Pruijn, G.J.M. PY - 2015 UR - https://hdl.handle.net/2066/150327 TI - RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences EP - 3050 SN - 2218-273X IS - iss. 4 SP - 3029 JF - Biomolecules VL - vol. 5 DO - https://doi.org/10.3390/biom5043029 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/150327/150327.pdf?sequence=1 ER - TY - JOUR AU - Polachek, A. AU - Egberts, W.V. AU - Fireman, E. AU - Paran, D. AU - Drokman, I. AU - Kaufman, I. AU - Wigler, I. AU - Caspi, D. AU - Levartovsky, D. AU - Pruijn, G.J.M. AU - Elkayam, O. PY - 2014 UR - https://hdl.handle.net/2066/140072 TI - Sputum anti-citrullinated protein antibodies in patients with long standing rheumatoid arthritis EP - S189 SN - 2326-5191 IS - iss. S10 SP - S189 JF - Arthritis & Rheumatology VL - vol. 66 DO - http://dx.doi.org/10.1002/art.38914 ER - TY - JOUR AU - Kappel, R.M. AU - Tervaert, J.W.C. AU - Pruijn, G.J.M. PY - 2014 UR - http://repository.ubn.ru.nl/handle/2066/128314 TI - Autoimmune/inflammatory syndrome induced by adjuvants (asia) due to silicone implant incompatibility syndrome in three sisters EP - 258 SN - 0392-856X IS - iss. 2 SP - 256 JF - Clinical and Experimental Rheumatology VL - vol. 32 ER - TY - JOUR AU - Assohou-Luty, C. AU - Raijmakers, R. AU - Benckhuijsen, W.E. AU - Stammen-Vogelzangs, J. AU - Ru, A. de AU - Veelen, P.A. van AU - Franken, K.L.M.C. AU - Drijfhout, J.W. AU - Pruijn, G.J.M. PY - 2014 UR - http://repository.ubn.ru.nl/handle/2066/128437 TI - The human peptidylarginine deiminases type 2 and type 4 have distinct substrate specificities EP - 836 SN - 1570-9639 IS - iss. 4 SP - 829 JF - Biochimica et Biophysica Acta. Proteins and Proteomics VL - vol. 1844 DO - http://dx.doi.org/10.1016/j.bbapap.2014.02.019 ER - TY - JOUR AU - Hensen, S.M.M. AU - Pruijn, G.J.M. PY - 2014 UR - http://repository.ubn.ru.nl/handle/2066/128404 TI - Methods for the detection of peptidylarginine deiminase (pad) activity and protein citrullination EP - 396 SN - 1535-9476 IS - iss. 2 SP - 388 JF - Molecular & Cellular Proteomics VL - vol. 13 DO - http://dx.doi.org/10.1074/mcp.R113.033746 ER - TY - JOUR AU - Joshua, V. AU - Schobers, L. AU - Israelsson, L. AU - Hansson, M. AU - Ronnelid, J. AU - Catrina, A.I. AU - Pruijn, G.J.M. AU - Malmstrom, V. PY - 2014 UR - https://hdl.handle.net/2066/133254 TI - B-cell responses to de novo identified citrullinated fibrinogen peptides are associated with the ptpn22 risk allele EP - 55 SN - 0300-9742 SP - 55 JF - Scandinavian Journal of Rheumatology VL - vol. 43 ER - TY - JOUR AU - Damgaard, D. AU - Palarasah, Y. AU - Skjodt, K. AU - Catrina, A.I. AU - Hensen, S.M.M. AU - Pruijn, G.J.M. AU - Nielsen, C.H. PY - 2014 UR - https://hdl.handle.net/2066/133196 TI - Generation of monoclonal antibodies against peptidylarginine deiminase 2 (pad2) and development of a pad2-specific enzyme-linked immunosorbent assay EP - 22 SN - 0022-1759 SP - 15 JF - Journal of Immunological Methods VL - vol. 405 DO - http://dx.doi.org/10.1016/j.jim.2013.12.008 ER - TY - JOUR AU - Niemela, E.H. AU - Oghabian, A. AU - Staals, R.H. AU - Greco, D. AU - Pruijn, G.J.M. AU - Frilander, M.J. PY - 2014 UR - https://hdl.handle.net/2066/133197 TI - Global analysis of the nuclear processing of transcripts with unspliced u12-type introns by the exosome EP - 7369 SN - 0305-1048 IS - iss. 11 SP - 7358 JF - Nucleic Acids Research VL - vol. 42 DO - http://dx.doi.org/10.1093/nar/gku391 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/133197/133197.pdf?sequence=1 ER - TY - JOUR AU - Boelens, W.C. PY - 2014 UR - https://hdl.handle.net/2066/131470 TI - Cell biological roles of alpha b-crystallin EP - 10 SN - 0079-6107 IS - iss. 1 SP - 3 JF - Progress in Biophysics and Molecular Biology VL - vol. 115 DO - http://dx.doi.org/10.1016/j.pbiomolbio.2014.02.005 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2014 UR - http://repository.ubn.ru.nl/handle/2066/128385 TI - How citrullination invaded rheumatoid arthritis research SN - 1478-6354 IS - iss. 1 JF - Arthritis Research & Therapy VL - vol. 16 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/128385/128385.pdf?sequence=1 ER - TY - JOUR AU - Bonger, K.M. AU - Rakhit, R. AU - Payumo, A.Y. AU - Chen, J.K. AU - Wandless, T.J. PY - 2014 UR - http://repository.ubn.ru.nl/handle/2066/128381 TI - General method for regulating protein stability with light EP - 115 SN - 1554-8929 IS - iss. 1 SP - 111 JF - Acs Chemical Biology VL - vol. 9 DO - http://dx.doi.org/10.1021/cb400755b ER - TY - JOUR AU - Aquino, S.G. de AU - Abdollahi-Roodsaz, S. AU - Koenders, M.I. AU - Loo, F.A.J. van de AU - Pruijn, G. AU - Marijnissen, R.J. AU - Walgreen, B. AU - Helsen, M.M.A. AU - Bersselaar, L.A.M. van den AU - Molon, R.S. de AU - Avila Campos, M.J. AU - Cunha, F.Q. AU - Cirelli, J.A. AU - Berg, W.B. van den PY - 2014 UR - https://hdl.handle.net/2066/136500 AB - Increasing epidemiologic evidence supports a link between periodontitis and rheumatoid arthritis. The actual involvement of periodontitis in the pathogenesis of rheumatoid arthritis and the underlying mechanisms remain, however, poorly understood. We investigated the influence of concomitant periodontitis on clinical and histopathologic characteristics of T cell-mediated experimental arthritis and evaluated modulation of type II collagen (CII)-reactive Th cell phenotype as a potential mechanism. Repeated oral inoculations of periodontal pathogens Porphyromonas gingivalis and Prevotella nigrescens induced periodontitis in mice, as evidenced by alveolar bone resorption. Interestingly, concurrent periodontitis induced by both bacteria significantly aggravated the severity of collagen-induced arthritis. Exacerbation of arthritis was characterized by increased arthritic bone erosion, whereas cartilage damage remained unaffected. Both P. gingivalis and P. nigrescens skewed the CII-specific T cell response in lymph nodes draining arthritic joints toward the Th17 phenotype without affecting Th1. Importantly, the levels of IL-17 induced by periodontal pathogens in CII-specific T cells directly correlated with the intensity of arthritic bone erosion, suggesting relevance in pathology. Furthermore, IL-17 production was significantly correlated with periodontal disease-induced IL-6 in lymph node cell cultures. The effects of the two bacteria diverged in that P. nigrescens, in contrast to P. gingivalis, suppressed the joint-protective type 2 cytokines, including IL-4. Further in vitro studies showed that the Th17 induction strongly depended on TLR2 expression on APCs and was highly promoted by IL-1. Our data provide evidence of the involvement of periodontitis in the pathogenesis of T cell-driven arthritis through induction of Ag-specific Th17 response. TI - Periodontal pathogens directly promote autoimmune experimental arthritis by inducing a TLR2- and IL-1-driven Th17 response EP - 4111 SN - 0022-1767 IS - iss. 9 SP - 4103 JF - Journal of Immunology VL - vol. 192 DO - http://dx.doi.org/10.4049/jimmunol.1301970 ER - TY - JOUR AU - Schootbrugge, C. van de AU - Schults, E.M. AU - Bussink, J. AU - Span, P.N. AU - Grenman, R. AU - Pruijn, G.J. AU - Kaanders, J.H. AU - Boelens, W.C. PY - 2014 UR - https://hdl.handle.net/2066/133189 TI - Effect of hypoxia on the expression of alphab-crystallin in head and neck squamous cell carcinoma SN - 1471-2407 JF - BMC Cancer VL - vol. 14 DO - https://doi.org/10.1186/1471-2407-14-252 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/133189/133189.pdf?sequence=1 ER - TY - JOUR AU - Schootbrugge, C. van de AU - Schults, E.M. AU - Bussink, J. AU - Span, P.N. AU - Grenman, R. AU - Pruijn, G.J.M. AU - Kaanders, J.H.A.M. AU - Boelens, W.C. PY - 2014 UR - https://hdl.handle.net/2066/137883 AB - BACKGROUND: The presence of hypoxia in head and neck squamous cell carcinoma (HNSCC) is associated with therapeutic resistance and increased risk of metastasis formation. alphaB-crystallin (HspB5) is a small heat shock protein, which is also associated with metastasis formation in HNSCC. In this study, we investigated whether alphaB-crystallin protein expression is increased in hypoxic areas of HNSCC biopsies and analyzed whether hypoxia induces alphaB-crystallin expression in vitro and in this way may confer hypoxic cell survival. METHODS: In 38 HNSCC biopsies, the overlap between immunohistochemically stained alphaB-crystallin and pimonidazole-adducts (hypoxiamarker) was determined. Moreover, expression levels of alphaB-crystallin were analyzed in HNSCC cell lines under hypoxia and reoxygenation conditions and after exposure to reactive oxygen species (ROS) and the ROS scavenger N-acetylcysteine (NAC). siRNA-mediated knockdown was used to determine the influence of alphaB-crystallin on cell survival under hypoxic conditions. RESULTS: In all biopsies alphaB-crystallin was more abundantly present in hypoxic areas than in normoxic areas. Remarkably, hypoxia decreased alphaB-crystallin mRNA expression in the HNSCC cell lines. Only after reoxygenation, a condition that stimulates ROS formation, alphaB-crystallin expression was increased. alphaB-crystallin mRNA levels were also increased by extracellular ROS, and NAC abolished the reoxygenation-induced alphaB-crystallin upregulation. Moreover, it was found that decreased alphaB-crystallin levels reduced cell survival under hypoxic conditions. CONCLUSIONS: We provide the first evidence that hypoxia stimulates upregulation of alphaB-crystallin in HNSCC. This upregulation was not caused by the low oxygen pressure, but more likely by ROS formation. The higher expression of alphaB-crystallin may lead to prolonged survival of these cells under hypoxic conditions. TI - Effect of hypoxia on the expression of alphaB-crystallin in head and neck squamous cell carcinoma SN - 1471-2407 JF - BMC Cancer VL - vol. 14 DO - https://doi.org/10.1186/1471-2407-14-252 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/137883/137883.pdf?sequence=1 ER - TY - JOUR AU - Mansson, C. AU - Arosio, P. AU - Hussein, R. AU - Kampinga, H.H. AU - Hashem, R.M. AU - Boelens, W.C. AU - Dobson, C.M. AU - Knowles, T.P. AU - Linse, S. AU - Emanuelsson, C. PY - 2014 UR - https://hdl.handle.net/2066/133205 TI - Interaction of the molecular chaperone dnajb6 with growing amyloid-beta 42 (abeta42) aggregates leads to sub-stoichiometric inhibition of amyloid formation EP - 31076 SN - 1083-351X IS - iss. 45 SP - 31066 JF - Journal of Biological Chemistry VL - vol. 289 DO - https://doi.org/10.1074/jbc.M114.595124 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/133205/133205.pdf?sequence=1 ER - TY - JOUR AU - Hensen, S.M.M. AU - Derksen, M. AU - Pruijn, G.J.M. PY - 2014 UR - https://hdl.handle.net/2066/133215 TI - Multiplex peptide-based b cell epitope mapping EP - 308 SN - 1064-3745 SP - 295 JF - Methods in Molecular Biology VL - vol. 1184 DO - https://doi.org/10.1007/978-1-4939-1115-8_16 ER - TY - JOUR AU - Joshua, V. AU - Schobers, L. AU - Israelsson, L. AU - Ronnelid, J. AU - Hansson, M. AU - Catrina, A.I. AU - Pruijn, G.J.M. AU - Malmstrom, V. PY - 2013 UR - https://hdl.handle.net/2066/120105 TI - B-Cell Responses To De Novo Identified Citrullinated Fibrinogen Peptides Are Associated With PTPN22 Risk Allele EP - S392 SN - 0004-3591 SP - S392 JF - Arthritis and Rheumatism VL - vol. 65 ER - TY - JOUR AU - Hensen, S.M.M. AU - Heldens, L. AU - Enckevort, C.M.W. van AU - Genesen, S.T. van AU - Pruijn, G.J.M. AU - Lubsen, N.H. PY - 2013 UR - https://hdl.handle.net/2066/111502 TI - Activation of the antioxidant response in methionine deprived human cells results in an HSF1-independent increase in HSPA1A mRNA levels EP - 1251 SN - 0300-9084 IS - iss. 6 SP - 1245 JF - Biochimie VL - vol. 95 DO - http://dx.doi.org/10.1016/j.biochi.2013.01.017 ER - TY - JOUR AU - Hensen, S.M. AU - Heldens, L. AU - Genesen, S.T. van AU - Pruijn, G.J.M. AU - Lubsen, N.H. PY - 2013 UR - https://hdl.handle.net/2066/122421 TI - A delayed antioxidant response in heat-stressed cells expressing a non-DNA binding HSF1 mutant EP - 473 SN - 1355-8145 IS - iss. 4 SP - 455 JF - Cell Stress & Chaperones VL - vol. 18 N1 - 10 juni 2013 DO - http://dx.doi.org/10.1007/s12192-012-0400-0 ER - TY - JOUR AU - Beers, J.J.B.C. van AU - Zendman, A.J.W. AU - Raijmakers, R. AU - Stammen-Vogelzangs, J. AU - Pruijn, G.J.M. PY - 2013 UR - https://hdl.handle.net/2066/111392 TI - Peptidylarginine deiminase expression and activity in PAD2 knock-out and PAD4-low mice EP - 308 SN - 0300-9084 IS - iss. 2 SP - 299 JF - Biochimie VL - vol. 95 DO - http://dx.doi.org/10.1016/j.biochi.2012.09.029 ER - TY - JOUR AU - Beers, J.J.B.C. van AU - Schwarte, C.M. AU - Stammen-Vogelzangs, J. AU - Oosterink, E. AU - Bozic, B. AU - Pruijn, G.J.M. PY - 2013 UR - https://hdl.handle.net/2066/111447 TI - The rheumatoid arthritis synovial fluid citrullinome reveals novel citrullinated epitopes in apolipoprotein E, myeloid nuclear differentiation antigen, and ss-actin EP - 80 SN - 0004-3591 IS - iss. 1 SP - 69 JF - Arthritis and Rheumatism VL - vol. 65 DO - http://dx.doi.org/10.1002/art.37720 ER - TY - JOUR AU - Sloan, K.E. AU - Mattijssen, S. AU - Lebaron, S. AU - Tollervey, D. AU - Pruijn, G.J.M. AU - Watkins, N.J. PY - 2013 UR - https://hdl.handle.net/2066/117332 TI - Both endonucleolytic and exonucleolytic cleavage mediate ITS1 removal during human ribosomal RNA processing EP - 588 SN - 0021-9525 IS - iss. 5 SP - 577 JF - Journal of Cell Biology VL - vol. 200 DO - http://dx.doi.org/10.1083/jcb.201207131 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/117332/117332.pdf?sequence=1 ER - TY - JOUR AU - Pruijn, G.J.M. PY - 2013 UR - https://hdl.handle.net/2066/123080 TI - Lichamelijke perfectie dankzij of ondanks siliconen EP - 13 IS - iss. 3 SP - 10 JF - Foliolum VL - vol. 26 ER - TY - JOUR AU - Hensen, S.M.M. AU - Beers, J.J.B.C. van AU - Boelens, W.C. AU - Pruijn, G.J.M. PY - 2013 UR - http://repository.ubn.ru.nl/handle/2066/128453 TI - Characterization of citrullinated proteins in complex samples EP - 196 SN - 0003-4967 SP - 196 JF - Annals of the Rheumatic Diseases VL - vol. 72 ER - TY - JOUR AU - Schootbrugge, C. van de AU - Asten, F. van AU - Nagtegaal, I.D. AU - Versleijen-Jonkers, Y.M. AU - Laarhoven, H.W. van AU - Roeffen, M.H. AU - Sweep, F.C. AU - Bussink, J. AU - Kaanders, J.H. AU - Pruijn, G.J.M. AU - Boelens, W.C. AU - Span, P.N. PY - 2013 UR - https://hdl.handle.net/2066/117335 TI - alphaB-crystallin expression is correlated with phospho-ERK1/2 expression in human breast cancer SN - 0393-6155 SP - 0 JF - International Journal of Biological Markers DO - http://dx.doi.org/10.5301/jbm.5000032 ER - TY - JOUR AU - Schootbrugge, C. van de AU - Bussink, J. AU - Span, P.N. AU - Sweep, C.G.J. AU - Grenman, R. AU - Stegeman, H. AU - Pruijn, G.J.M. AU - Kaanders, J.H.A.M. AU - Boelens, W.C. PY - 2013 UR - https://hdl.handle.net/2066/111464 TI - alphaB-crystallin stimulates VEGF secretion and tumor cell migration and correlates with enhanced distant metastasis in head and neck squamous cell carcinoma SN - 1471-2407 JF - BMC Cancer VL - vol. 13 DO - https://doi.org/10.1186/1471-2407-13-128 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/111464/111464.pdf?sequence=1 ER - TY - JOUR AU - Caron, M.M. AU - Steinbusch, M. AU - Reicherter, K. AU - Mattijssen, S. AU - Surtel, D.A. AU - Rhijn, L.W. van AU - Pruijn, G.J.M. AU - Lausch, E. AU - Zabel, B. AU - Welting, T.J. PY - 2013 UR - https://hdl.handle.net/2066/117187 TI - RNase MRP is a novel regulator of endochondral ossification EP - S13 SN - 1063-4584 SP - S12 JF - Osteoarthritis and Cartilage VL - vol. 21 ER - TY - JOUR AU - Chirivi, R.G.S. AU - Rosmalen, J.W.G. van AU - Jenniskens, G.J. AU - Pruijn, G.J.M. AU - Raats, J.M.H. PY - 2013 UR - https://hdl.handle.net/2066/123057 TI - Citrullination: A target for disease intervention in multiple sclerosis and other inflammatory diseases? SN - 2155-9899 SP - 146 JF - Journal of Clinical & Cellular Immunology VL - vol. 4 ER - TY - JOUR AU - Engelsman, J. den AU - Schootbrugge, C. van de AU - Yong, J. AU - Pruijn, G.J.M. AU - Boelens, W.C. PY - 2013 UR - https://hdl.handle.net/2066/117182 TI - Pseudophosphorylated alphaB-crystallin is a nuclear chaperone imported into the nucleus with help of the SMN complex SN - 1932-6203 IS - iss. 9 JF - PLoS One VL - vol. 8 DO - https://doi.org/10.1371/journal.pone.0073489 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/117182/117182.pdf?sequence=1 ER - TY - JOUR AU - Beers, J.J.B.C. van AU - Willemze, A. AU - Jansen, J.J. AU - Engbers, G.H.M. AU - Salden, M. AU - Raats, J. AU - Drijfhout, J.W. AU - Mil, A.H.V. van der Helm-va AU - Toes, R.E.M. AU - Pruijn, G.J.M. PY - 2013 UR - http://repository.ubn.ru.nl/handle/2066/128452 TI - Acpa fine-specificity profiles in early rheumatoid arthritis patients do not correlate with clinical features at baseline or with disease progression SN - 1478-6354 IS - iss. 5 JF - Arthritis Research & Therapy VL - vol. 15 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/128452/128452.pdf?sequence=1 ER - TY - JOUR AU - Beers, J.J.B.C. van AU - Willemze, A. AU - Jansen, J.J. AU - Engbers, G.H.M. AU - Salden, M. AU - Raats, J. AU - Drijfhout, J.W. AU - Helm-van Mil, A.H.M. AU - Toes, R.E. AU - Pruijn, G.J. PY - 2013 UR - https://hdl.handle.net/2066/223922 TI - Acpa fine-specificity profiles in early rheumatoid arthritis patients do not correlate with clinical features at baselinen nor with disease progression SN - 1478-6354 IS - iss. 5 SP - R140 JF - Arthritis Research & Therapy VL - vol. 15 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/223922/223922.pdf?sequence=1 ER - TY - JOUR AU - Pluk, H. AU - Hoeve, B.J.A. van AU - Dooren, S.H. van AU - Stammen-Vogelzangs, J. AU - Heijden, A. van der AU - Schelhaas, H.J. AU - Verbeek, M.M. AU - Badrising, U.A. AU - Arnardottir, S. AU - Gheorghe, K. AU - Lundberg, I.E. AU - Boelens, W.C. AU - Engelen, B.G.M. van AU - Pruijn, Ger PY - 2013 UR - https://hdl.handle.net/2066/117143 AB - OBJECTIVE: Sporadic inclusion body myositis (sIBM) is an inflammatory myopathy characterized by both degenerative and autoimmune features. In contrast to other inflammatory myopathies, myositis-specific autoantibodies had not been found in sIBM patients until recently. We used human skeletal muscle extracts as a source of antigens to detect autoantibodies in sIBM and to characterize the corresponding antigen. METHODS: Autoantibodies to skeletal muscle antigens were detected by immunoblotting. The target antigen was immunoaffinity-purified from skeletal muscle extracts and characterized by mass spectrometry. A cDNA encoding this protein was cloned and expressed in vitro, and its recognition by patient sera was analyzed in an immunoprecipitation assay. Epitopes were mapped using microarrays of overlapping peptides. RESULTS: An Mr 44,000 polypeptide (Mup44) was frequently targeted by sIBM autoantibodies. The target protein was purified, and subsequent mass spectrometry analysis revealed that Mup44 is the cytosolic 5'-nucleotidase 1A (cN1A). By immunoprecipitation of recombinant cN1A, high concentrations of anti-Mup44 autoantibodies were detected in 33% of sIBM patient sera, whereas their prevalence in dermatomyositis, polymyositis, and other neuromuscular disorders appeared to be rare (4.2%, 4.5%, and 3.2%, respectively). Low concentrations of anti-Mup44 antibodies were found in myositis as well as other neuromuscular disorders, but not in healthy controls. Three major autoepitope regions of cN1A were mapped by using microarrays containing a set of overlapping peptides covering the complete cN1A amino acid sequence. INTERPRETATION: Anti-Mup44 autoantibodies, which are targeted to cN1A, represent the first serological biomarker for sIBM and may facilitate the diagnosis of this type of myositis. TI - Autoantibodies to cytosolic 5'-nucleotidase 1A in inclusion body myositis EP - 407 SN - 0364-5134 IS - iss. 3 SP - 397 JF - Annals of Neurology VL - vol. 73 DO - https://doi.org/10.1002/ana.23822 ER - TY - JOUR AU - Schootbrugge, C. van de AU - Bussink, J. AU - Span, P.N. AU - Sweep, C.G.J. AU - Grenman, R. AU - Stegeman, H. AU - Pruijn, G.J.M. AU - Kaanders, J.H.A.M. AU - Boelens, W.C. PY - 2013 UR - https://hdl.handle.net/2066/141895 AB - BACKGROUND: alphaB-crystallin is able to modulate vascular endothelial growth factor (VEGF) secretion. In many solid tumors VEGF is associated with angiogenesis, metastasis formation and poor prognosis. We set out to assess whether alphaB-crystallin expression is correlated with worse prognosis and whether this is related to VEGF secretion and cell motility in head and neck squamous cell carcinoma (HNSCC). METHODS: alphaB-crystallin expression was determined immunohistochemically in tumor biopsies of 38 HNSCC patients. Locoregional control (LRC) and metastasis-free survival (MFS) of the patients were analyzed in relation to alphaB-crystallin expression. Additionally, the effects of alphaB-crystallin knockdown on VEGF secretion and cell motility were studied in vitro. RESULTS: Patients with higher staining fractions of alphaB-crystallin exhibited a significantly shorter MFS (Log-Rank test, p < 0.005). Under normoxic conditions alphaB-crystallin knockdown with two different siRNAs in a HNSCC cell line reduced VEGF secretion 1.9-fold and 2.1-fold, respectively. Under hypoxic conditions, a similar reduction of VEGF secretion was observed, 1.9-fold and 2.2-fold, respectively. The effect on cell motility was assessed by a gap closure assay, which showed that alphaB-crystallin knockdown decreased the rate by which HNSCC cells were able to close a gap by 1.5- to 2.0-fold. CONCLUSIONS: Our data suggest that alphaB-crystallin expression is associated with distant metastases formation in HNSCC patients. This association might relate to the chaperone function of alphaB-crystallin in mediating folding and secretion of VEGF and stimulating cell migration. TI - alphaB-crystallin stimulates VEGF secretion and tumor cell migration and correlates with enhanced distant metastasis in head and neck squamous cell carcinoma SN - 1471-2407 JF - BMC Cancer VL - vol. 13 DO - https://doi.org/10.1186/1471-2407-13-128 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/141895/141895.pdf?sequence=1 ER - TY - JOUR AU - Schootbrugge, C. van de AU - Asten, F van AU - Nagtegaal, I.D. AU - Versleijen-Jonkers, Y.M.H. AU - Laarhoven, H.W.M. van AU - Roeffen, M.H.S. AU - Sweep, C.G.J. AU - Bussink, J. AU - Kaanders, J.H.A.M. AU - Pruijn, G.J.M. AU - Boelens, W.C. AU - Span, P.N. PY - 2013 UR - https://hdl.handle.net/2066/125273 AB - alphaB-crystallin is regarded as a biomarker for triple-negative and/or basal-like breast cancer. In normal breast cells, overexpression of alphaB-crystallin leads to neoplastic-like changes, which likely relate to enhanced expression of phosphorylated ERK1/2 (pERK1/2). In this study, we investigated whether alphaB-crystallin expression is correlated to pERK1/2 expression in breast cancer. In a balanced tissue microarray the expression of alphaB-crystallin and pERK1/2 kinase were determined immunohistochemically, together with the triple-negativity and basal-like markers CK5/6 and SMA and the signaling molecules pAKT, pmTOR, EGFR, and IGF-1R. alphaB-crystallin expression significantly correlated with triple negativity and basal-like markers CK5/6 and SMA (Pearson Chi-square test p=0.004, p=0.001, and p<0.001, respectively). A significant correlation was also observed with pERK1/2 expression (p=0.002). siRNA-mediated knockdown of alphaB-crystallin in the triple-negative breast cell line MDA-MB468 did not show an effect on pERK1/2 expression levels, indicating that lowering the level of alphaB-crystallin does not reduce pERK1/2 expression. Our results confirm that alphaB-crystallin can be used as a biomarker for triple-negative and/or basal-like breast cancer. The expression of alphaB-crystallin correlates with pERK1/2 expression in breast cancer tissue suggesting that therapies targeting alphaB-crystallin might be considered for treatment of triple-negative or basal-like breast cancer. TI - alphaB-crystallin expression is correlated with phospho-ERK1/2 expression in human breast cancer EP - 70 SN - 0393-6155 IS - iss. 4 SP - e365 JF - International Journal of Biological Markers VL - vol. 28 DO - https://doi.org/10.5301/JBM.5000032 ER - TY - JOUR AU - Hensen, S.M.M. AU - Heldens, L. AU - Enckevort, C.M.W. van AU - Genesen, S.T. van AU - Pruijn, G.J.M. AU - Lubsen, N.H. PY - 2012 UR - https://hdl.handle.net/2066/103412 TI - Heat shock factor 1 is inactivated by amino acid deprivation EP - 755 SN - 1355-8145 IS - iss. 6 SP - 743 JF - Cell Stress & Chaperones VL - vol. 17 DO - http://dx.doi.org/10.1007/s12192-012-0347-1 ER - TY - JOUR AU - Beers, J.J.B.C. van AU - Willemze, A. AU - Stammen-Vogelzangs, J. AU - Drijfhout, J.W. AU - Toes, R.E.M. AU - Pruijn, G.J.M. PY - 2012 UR - https://hdl.handle.net/2066/93755 TI - Anti-citrullinated fibronectin antibodies in rheumatoid arthritis are associated with human leukocyte antigen-drb1 shared epitope alleles SN - 1478-6354 IS - iss. 1 JF - Arthritis Research & Therapy VL - vol. 14 DO - http://dx.doi.org/10.1186/ar3744 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/93755/93755.pdf?sequence=1 ER - TY - JOUR AU - Astuti, D. AU - Morris, M.R. AU - Cooper, W.N. AU - Staals, R.H.J. AU - Wake, N.C. AU - Fews, G.A. AU - Gill, H. AU - Gentle, D. AU - Shuib, S. AU - Ricketts, C.J. AU - Cole, T. AU - Essen, A.J. van AU - Lingen, R.A. van AU - Neri, G. AU - Opitz, J.M. AU - Rump, P. AU - Stolte-Dijkstra, I. AU - Muller, F. AU - Pruijn, G.J.M. AU - Latif, F. AU - Maher, E.R. PY - 2012 UR - https://hdl.handle.net/2066/93876 TI - Germline mutations in dis3l2 cause the perlman syndrome of overgrowth and wilms tumor susceptibility EP - 284 SN - 1061-4036 IS - iss. 3 SP - 277 JF - Nature Genetics VL - vol. 44 DO - http://dx.doi.org/10.1038/ng.1071 ER - TY - JOUR AU - Makrygiannakis, D. AU - Revu, S. AU - Engstrom, M. AU - Klint, E. Af AU - Nicholas, A.P. AU - Pruijn, G.J.M. AU - Catrina, A.I. PY - 2012 UR - https://hdl.handle.net/2066/93927 TI - Local administration of glucocorticoids decreases synovial citrullination in rheumatoid arthritis SN - 1478-6354 IS - iss. 1 JF - Arthritis Research & Therapy VL - vol. 14 DO - http://dx.doi.org/10.1186/ar3702 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/93927/93927.pdf?sequence=1 ER - TY - JOUR AU - Geel, R. van AU - Pruijn, G.J.M. AU - Delft, F.L. van AU - Boelens, W.C. PY - 2012 UR - https://hdl.handle.net/2066/94017 TI - Preventing thiol-yne addition improves the specificity of strain-promoted azide-alkyne cycloaddition EP - 398 SN - 1043-1802 IS - iss. 3 SP - 392 JF - Bioconjugate Chemistry VL - vol. 23 DO - http://dx.doi.org/10.1021/bc200365k ER - TY - JOUR AU - Geel, R. van AU - Debets, M.F. AU - Lowik, D.W.P.M. AU - Pruijn, G.J.M. AU - Boelens, W.C. PY - 2012 UR - https://hdl.handle.net/2066/103290 TI - Detection of transglutaminase activity using click chemistry EP - 1263 SN - 0939-4451 IS - iss. 3 SP - 1251 JF - Amino Acids VL - vol. 43 DO - http://dx.doi.org/10.1007/s00726-011-1198-2 ER - TY - JOUR AU - Raijmakers, R. AU - Beers, J.J. van AU - El-Azzouny, M. AU - Visser, N.F. AU - Bozic, B. AU - Pruijn, G.J.M. AU - Heck, A.J. PY - 2012 UR - https://hdl.handle.net/2066/103338 TI - Elevated levels of fibrinogen-derived endogenous citrullinated peptides in synovial fluid of rheumatoid arthritis patients SN - 1478-6354 IS - iss. 3 SP - R114 JF - Arthritis Research & Therapy VL - vol. 14 DO - http://dx.doi.org/10.1186/ar3840 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/103338/103338.pdf?sequence=1 ER - TY - JOUR AU - Beers, J.J. van AU - Zendman, A.J. AU - Raijmakers, R. AU - Stammen-Vogelzangs, J. AU - Pruijn, G.J.M. PY - 2012 UR - https://hdl.handle.net/2066/103611 TI - Peptidylarginine deiminase expression and activity in pad2 knock-out and pad4-low mice SN - 0300-9084 JF - Biochimie DO - http://dx.doi.org/10.1016/j.biochi.2012.09.029 ER - TY - JOUR AU - Heldens, L. AU - Genesen, S.T. van AU - Hanssen, L.L.P. AU - Hageman, J. AU - Kampinga, H.H. AU - Lubsen, N.H. PY - 2012 UR - https://hdl.handle.net/2066/103655 TI - Protein refolding in peroxisomes is dependent upon an HSF1-regulated function EP - 613 SN - 1355-8145 IS - iss. 5 SP - 603 JF - Cell Stress & Chaperones VL - vol. 17 DO - http://dx.doi.org/10.1007/s12192-012-0335-5 ER - TY - JOUR AU - Kappel, R.M. AU - Pruijn, G.J.M. PY - 2012 UR - https://hdl.handle.net/2066/103831 TI - The monobloc hydrogel breast implant, experiences and ideas EP - 233 SN - 0930-343X IS - iss. 3 SP - 229 JF - European Journal of Plastic Surgery VL - vol. 35 DO - http://dx.doi.org/10.1007/s00238-011-0591-0 ER - TY - JOUR AU - Clark, A.R. AU - Lubsen, N.H. AU - Slingsby, C PY - 2012 UR - https://hdl.handle.net/2066/103896 TI - sHSP in the eye lens: Crystallin mutations, cataract and proteostasis EP - 1697 SN - 1357-2725 IS - iss. 10 SP - 1687 JF - International Journal of Biochemistry & Cell Biology VL - vol. 44 DO - http://dx.doi.org/10.1016/j.biocel.2012.02.015 ER - TY - JOUR AU - Mohamed, B.M. AU - Verma, N.K. AU - Davies, A.M. AU - McGowan, A. AU - Crosbie-Staunton, K. AU - Prina-Mello, A. AU - Kelleher, D. AU - Botting, C.H. AU - Causey, C.P. AU - Thompson, P.R. AU - Pruijn, G.J.M. AU - Kisin, E.R. AU - Tkach, A.V. AU - Shvedova, A.A. AU - Volkov, Y. PY - 2012 UR - https://hdl.handle.net/2066/103227 TI - Citrullination of proteins: A common post-translational modification pathway induced by different nanoparticles in vitro and in vivo EP - 1195 SN - 1743-5889 IS - iss. 8 SP - 1181 JF - Nanomedicine VL - vol. 7 DO - http://dx.doi.org/10.2217/nnm.11.177 ER - TY - JOUR AU - Mattijssen, S. AU - Pruijn, G.J.M. PY - 2012 UR - https://hdl.handle.net/2066/94161 TI - Viperin, a key player in the antiviral response EP - 426 SN - 1286-4579 IS - iss. 5 SP - 419 JF - Microbes and Infection VL - vol. 14 DO - http://dx.doi.org/10.1016/j.micinf.2011.11.015 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Beers, J.J.B.C. van AU - Pruijn, G.J.M. PY - 2011 UR - https://hdl.handle.net/2066/91562 TI - Anti-ccp antibodies: The past, the present and the future EP - 398 SN - 1759-4790 IS - iss. 7 SP - 391 JF - Nature Reviews. Rheumatology VL - vol. 7 PS - 8 p. DO - http://dx.doi.org/10.1038/nrrheum.2011.76 ER - TY - JOUR AU - Verhagen, A.P.M. AU - Pruijn, G.J.M. PY - 2011 UR - https://hdl.handle.net/2066/91569 TI - Are the ro rnp-associated y rnas concealing micrornas? Y rna-derived mirnas may be involved in autoimmunity EP - 682 SN - 0265-9247 IS - iss. 9 SP - 674 JF - Bioessays VL - vol. 33 PS - 9 p. DO - http://dx.doi.org/10.1002/bies.201100048 ER - TY - JOUR AU - Beers, J.J.B.C. van AU - Segbers-Lokate, A.M.C. AU - Egberts, W. AU - Schasfoort, R.B.M. AU - Pruijn, G.J.M. PY - 2011 UR - https://hdl.handle.net/2066/91909 TI - Identification of autoantibody profiles by monitoring autoantibody biomarkers in rheumatoid arthritis with microarray surface plasmon resonance imaging EP - S686 SN - 0004-3591 IS - iss. 10 SP - S685 JF - Arthritis and Rheumatism VL - vol. 63 PS - 2 p. ER - TY - JOUR AU - Schoffelen, S. AU - van Eldijk, M.B. AU - Rooijakkers, B. AU - Raijmakers, R. AU - Heck, A.J.R. AU - van Hest, J.C.M. PY - 2011 UR - https://hdl.handle.net/2066/92066 TI - Metal-free and ph-controlled introduction of azides in proteins EP - 705 SN - 2041-6520 IS - iss. 4 SP - 701 JF - Chemical Science VL - vol. 2 PS - 5 p. DO - http://dx.doi.org/10.1039/c0sc00562b ER - TY - JOUR AU - Dooren, S.H.J. van AU - Raijmakers, R. AU - Pluk, H. AU - Lokate, A.M.C. AU - Koemans, T.S. AU - Spanjers, R.E.C. AU - Heck, A.J.R. AU - Boelens, W.C. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2011 UR - https://hdl.handle.net/2066/92165 TI - Oxidative stress-induced modifications of histidyl-trna synthetase affect its trna aminoacylation activity but not its immunoreactivity EP - 553 SN - 0829-8211 IS - iss. 6 SP - 545 JF - Biochemistry and Cell Biology VL - vol. 89 PS - 9 p. DO - http://dx.doi.org/10.1139/O11-055 ER - TY - JOUR AU - Staals, R.H.J. AU - Pruijn, G.J.M. PY - 2011 UR - https://hdl.handle.net/2066/92465 TI - The human exosome and disease EP - 142 SN - 0065-2598 SP - 132 JF - Advances in Experimental Medicine and Biology VL - vol. 702 ER - TY - JOUR AU - Geelen, S.M. van AU - Fuchs, C.E. AU - Sinnema, G. AU - Putte, E.M. van de AU - Geel, R. van AU - Hermans, H.J.M. AU - Kuis, W. PY - 2011 UR - https://hdl.handle.net/2066/92330 TI - Self-investigation in adolescent chronic fatigue syndrome: Narrative changes and health improvement EP - 233 SN - 0738-3991 IS - iss. 2 SP - 227 JF - Patient Education and Counseling VL - vol. 83 PS - 7 p. DO - https://doi.org/10.1016/j.pec.2010.05.023 ER - TY - JOUR AU - Stadt, L.A. van de AU - Horst, A.R. van der AU - Koning, M.H.M.T. de AU - Bos, W.H. AU - Wolbink, G.J. AU - Stadt, R.J. van de AU - Pruijn, G.J.M. AU - Dijkmans, B.A.C. AU - Schaardenburg, D. van AU - Hamann, D. PY - 2011 UR - https://hdl.handle.net/2066/92460 TI - The extent of the anti-citrullinated protein antibody repertoire is associated with arthritis development in patients with seropositive arthralgia EP - 133 SN - 0003-4967 IS - iss. 1 SP - 128 JF - Annals of the Rheumatic Diseases VL - vol. 70 PS - 6 p. DO - https://doi.org/10.1136/ard.2010.132662 ER - TY - JOUR AU - Lambert, W. AU - Soderberg, C.A.G. AU - Rutsdottir, G. AU - Boelens, W.C. AU - Emanuelsson, C. PY - 2011 UR - https://hdl.handle.net/2066/92496 TI - Thiol-exchange in DTSSP crosslinked peptides is proportional to cysteine content and precisely controlled in crosslink detection by two-step LC-MALDI MSMS EP - 1691 SN - 0961-8368 IS - iss. 10 SP - 1682 JF - Protein Science VL - vol. 20 PS - 12 p. DO - http://dx.doi.org/10.1002/pro.699 DO - https://doi.org/10.1002/pro.699 ER - TY - JOUR AU - Canalle, L.A. AU - Vong, T. AU - Adams, P. AU - Delft, F.L. van AU - Raats, J.M.H. AU - Chirivi, R.G.S. AU - van Hest, J.C.M. PY - 2011 UR - https://hdl.handle.net/2066/91629 TI - Clickable enzyme-linked immunosorbent assay EP - 3697 SN - 1525-7797 IS - iss. 10 SP - 3692 JF - Biomacromolecules VL - vol. 12 PS - 6 p. DO - https://doi.org/10.1021/bm2009137 ER - TY - JOUR AU - Akker, J. van den AU - Bavel, E. Van AU - Geel, R. van AU - Matlung, H.L. AU - Tuna, B.G. AU - Janssen, G.M.C. AU - Veelen, P.A. van AU - Boelens, W.C. AU - Mey, J.G.R. De AU - Bakker, E.N.T.P. PY - 2011 UR - https://hdl.handle.net/2066/92483 TI - The redox state of transglutaminase 2 controls arterial remodeling SN - 1932-6203 IS - iss. 8 JF - PLoS One VL - vol. 6 DO - https://doi.org/10.1371/journal.pone.0023067 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/92483/92483.pdf?sequence=1 ER - TY - JOUR AU - Dooren, S.H.J. van AU - Venrooij, W.J.W. van AU - Pruijn, G.M. PY - 2011 UR - https://hdl.handle.net/2066/92105 TI - Myositis-specific autoantibodies: Detection and clinical associations EP - 20 SN - 2038-3274 IS - iss. 1 SP - 5 JF - Autoimmunity Highlights VL - vol. 2 PS - 16 p. ER - TY - JOUR AU - Heldens, L. AU - Hensen, S.M.M. AU - Onnekink, C. AU - Genesen, S.T. van AU - Dirks, R.P. AU - Lubsen, N.H. PY - 2011 UR - https://hdl.handle.net/2066/91546 TI - An Atypical Unfolded Protein Response in Heat Shocked Cells SN - 1932-6203 IS - iss. 8 JF - PLoS One VL - vol. 6 DO - https://doi.org/10.1371/journal.pone.0023512 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/91546/91546.pdf?sequence=1 ER - TY - JOUR AU - Mattijssen, S. AU - Hinson, E.R. AU - Onnekink, C. AU - Hermanns, P. AU - Zabel, B. AU - Cresswell, P. AU - Pruijn, G.J.M. PY - 2011 UR - https://hdl.handle.net/2066/92545 TI - Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease EP - 2480 SN - 1420-682X IS - iss. 14 SP - 2469 JF - Cellular and Molecular Life Sciences VL - vol. 68 PS - 12 p. DO - http://dx.doi.org/10.1007/s00018-010-0568-3 DO - https://doi.org/10.1007/s00018-010-0568-3 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/92545/92545.pdf?sequence=1 ER - TY - JOUR AU - Kappe, G. AU - Purkiss, A.G. AU - Genesen, S.T. van AU - Slingsby, C AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83594 TI - Explosive expansion of beta gamma-crystallin genes in the ancestral vertebrate (vol 71, 219, 2010) EP - 318 SN - 0022-2844 IS - iss. 4 SP - 317 JF - Journal of Molecular Evolution VL - vol. 71 PS - 2 p. DO - http://dx.doi.org/10.1007/s00239-010-9387-2 ER - TY - JOUR AU - Kappe, G. AU - Purkiss, A.G. AU - Genesen, S.T. van AU - Slingsby, C AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83598 TI - Explosive expansion of beta gamma-crystallin genes in the ancestral vertebrate EP - 230 SN - 0022-2844 IS - iss. 3 SP - 219 JF - Journal of Molecular Evolution VL - vol. 71 PS - 12 p. DO - http://dx.doi.org/10.1007/s00239-010-9379-2 ER - TY - JOUR AU - Pruijn, G.J.M. AU - Wiik, A. AU - van Venrooij, W.J. PY - 2010 UR - https://hdl.handle.net/2066/83817 TI - The use of citrullinated peptides and proteins for the diagnosis of rheumatoid arthritis SN - 1478-6354 IS - iss. 1 SP - 203 JF - Arthritis Research & Therapy VL - vol. 12 PS - 8 p. DO - http://dx.doi.org/10.1186/ar2903 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/83817/83817.pdf?sequence=1 ER - TY - JOUR AU - Hands-Taylor, K.L.D. AU - Martino, L. AU - Tata, R. AU - Babon, J.J. AU - Bui, T.T. AU - Drake, A.F. AU - Beavil, R.L. AU - Pruijn, G.J.M. AU - Brown, P.R. AU - Conte, M.R. PY - 2010 UR - https://hdl.handle.net/2066/84372 TI - Heterodimerization of the human rnase p/mrp subunits rpp20 and rpp25 is a prerequisite for interaction with the p3 arm of rnase mrp rna EP - 4066 SN - 0305-1048 IS - iss. 12 SP - 4052 JF - Nucleic Acids Research VL - vol. 38 PS - 15 p. DO - http://dx.doi.org/10.1093/nar/gkq141 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/84372/84372.pdf?sequence=1 ER - TY - JOUR AU - Wiik, A.S. AU - van Venrooij, W.J. AU - Pruijn, G.J.M. PY - 2010 UR - https://hdl.handle.net/2066/84451 TI - All you wanted to know about anti-ccp but were afraid to ask EP - 93 SN - 1568-9972 IS - iss. 2 SP - 90 JF - Autoimmunity Reviews VL - vol. 10 PS - 4 p. DO - http://dx.doi.org/10.1016/j.autrev.2010.08.009 ER - TY - JOUR AU - Staals, R.H.J. AU - Bronkhorst, A.W. AU - Schilders, G.W. AU - Slomovic, S. AU - Schuster, G. AU - Heck, A.J.R. van AU - Raijmakers, R. AU - Pruijn, G.J.M. PY - 2010 UR - https://hdl.handle.net/2066/83868 AB - The exosome is an exoribonuclease complex involved in the degradation and maturation of a wide variety of RNAs. The nine-subunit core of the eukaryotic exosome is catalytically inactive and may have an architectural function and mediate substrate binding. In Saccharomyces cerevisiae, the associated Dis3 and Rrp6 provide the exoribonucleolytic activity. The human exosome-associated Rrp6 counterpart contributes to its activity, whereas the human Dis3 protein is not detectably associated with the exosome. Here, a proteomic analysis of immunoaffinity-purified human exosome complexes identified a novel exosome-associated exoribonuclease, human Dis3-like exonuclease 1 (hDis3L1), which was confirmed to associate with the exosome core by co-immunoprecipitation. In contrast to the nuclear localization of Dis3, hDis3L1 exclusively localized to the cytoplasm. The hDis3L1 isolated from transfected cells degraded RNA in an exoribonucleolytic manner, and its RNB domain seemed to mediate this activity. The siRNA-mediated knockdown of hDis3L1 in HeLa cells resulted in elevated levels of poly(A)-tailed 28S rRNA degradation intermediates, indicating the involvement of hDis3L1 in cytoplasmic RNA decay. Taken together, these data indicate that hDis3L1 is a novel exosome-associated exoribonuclease in the cytoplasm of human cells. TI - Dis3-like 1: a novel exoribonuclease associated with the human exosome. EP - 2367 SN - 0261-4189 IS - iss. 14 SP - 2358 JF - EMBO Journal VL - vol. 29 PS - 10 p. DO - https://doi.org/10.1038/emboj.2010.122 ER - TY - JOUR AU - Smits, P. AU - Mattijssen, S. AU - Morava, E. AU - Brand, M. van den AU - Brandt, F. van den AU - Wijburg, F. AU - Pruijn, G.J.M. AU - Smeitink, J.A.M. AU - Nijtmans, L.G.J. AU - Rodenburg, R.J.T. AU - Heuvel, L.P.W.J. van den PY - 2010 UR - https://hdl.handle.net/2066/88552 AB - Combined oxidative phosphorylation (OXPHOS) system deficiencies are a group of mitochondrial disorders that are associated with a range of clinical phenotypes and genetic defects. They occur in approximately 30% of all OXPHOS disorders and around 4% are combined complex I, III and IV deficiencies. In this study we present two mutations in the mitochondrial tRNA(Trp) (MT-TW) and tRNA(Arg) (MT-TR) genes, m.5556G>A and m.10450A>G, respectively, which were detected in two unrelated patients showing combined OXPHOS complex I, III and IV deficiencies and progressive multisystemic diseases. Both mitochondrial tRNA mutations were almost homoplasmic in fibroblasts and muscle tissue of the two patients and not present in controls. Patient fibroblasts showed a general mitochondrial translation defect. The mutations resulted in lowered steady-state levels and altered conformations of the tRNAs. Cybrid cell lines showed similar tRNA defects and impairment of OXPHOS complex assembly as patient fibroblasts. Our results show that these tRNA(Trp) and tRNA(Arg) mutations cause the combined OXPHOS deficiencies in the patients, adding to the still expanding group of pathogenic mitochondrial tRNA mutations. TI - Functional consequences of mitochondrial tRNA Trp and tRNA Arg mutations causing combined OXPHOS defects. EP - 329 SN - 1018-4813 IS - iss. 3 SP - 324 JF - European Journal of Human Genetics VL - vol. 18 N1 - 1 maart 2010 DO - https://doi.org/10.1038/ejhg.2009.169 ER - TY - JOUR AU - Kappe, G. AU - Boelens, W.C. AU - de Jong, W.W. PY - 2010 UR - https://hdl.handle.net/2066/83639 TI - Why proteins without an alpha-crystallin domain should not be included in the human small heat shock protein family hspb EP - 461 SN - 1355-8145 IS - iss. 4 SP - 457 JF - Cell Stress & Chaperones VL - vol. 15 PS - 5 p. DO - http://dx.doi.org/10.1007/s12192-009-0155-4 ER - TY - JOUR AU - Dirks, R.P.H. AU - Geel, R. van AU - Hensen, S.M.M. AU - Genesen, S.T. van AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83587 TI - Manipulating heat shock factor-1 in xenopus tadpoles: Neuronal tissues are refractory to exogenous expression SN - 1932-6203 IS - iss. 4 JF - PLoS One VL - vol. 5 PS - 10 p. DO - https://doi.org/10.1371/journal.pone.0010158 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/83587/83587.pdf?sequence=1 ER - TY - JOUR AU - Slomovic, S. AU - Fremder, E. AU - Staals, R.H.G. AU - Pruijn, G.J.M. AU - Schuster, G. PY - 2010 UR - https://hdl.handle.net/2066/84302 TI - Addition of poly(a) and poly(a)-rich tails during RNA degradation in the cytoplasm of human cells EP - 7412 SN - 0027-8424 IS - iss. 16 SP - 7407 JF - Proceedings of the National Academy of Sciences USA VL - vol. 107 PS - 6 p. DO - https://doi.org/10.1073/pnas.0910621107 ER - TY - JOUR AU - Mattijssen, S. AU - Welting, T.J.M. AU - Pruijn, G.J.M. PY - 2010 UR - https://hdl.handle.net/2066/84120 TI - RNase MRP and disease EP - 116 SN - 1757-7012 IS - iss. 1 SP - 102 JF - Wiley Interdisciplinary Reviews: Rna VL - vol. 1 PS - 15 p. DO - https://doi.org/10.1002/wrna.9 ER - TY - JOUR AU - van der Woude, D. AU - Dahlqvist, S.R. AU - Ioan-Facsinay, A. AU - Onnekink, C. AU - Schwarte, C.M. AU - Verpoort, K.N. AU - Drijfhout, J.W. AU - Huizinga, T.W.J. AU - Toes, R.E.M. AU - Pruijn, G.J.M. PY - 2010 UR - https://hdl.handle.net/2066/83805 TI - Epitope spreading of the anti-citrullinated protein antibody response occurs before disease onset and is associated with the disease course of early arthritis EP - 1561 SN - 0003-4967 IS - iss. 8 SP - 1554 JF - Annals of the Rheumatic Diseases VL - vol. 69 DO - https://doi.org/10.1136/ard.2009.124537 ER - TY - JOUR AU - Heldens, L. AU - Dirks, R.P.H. AU - Hensen, S.M.M. AU - Onnekink, C. AU - Genesen, S.T. van AU - Rustenburg, F. AU - Lubsen, N.H. PY - 2010 UR - https://hdl.handle.net/2066/83516 TI - Co-chaperones are limiting in a depleted chaperone network EP - 4048 SN - 1420-682X IS - iss. 23 SP - 4035 JF - Cellular and Molecular Life Sciences VL - vol. 67 DO - https://doi.org/10.1007/s00018-010-0430-7 ER - TY - JOUR AU - Elkayam, O. AU - Segal, R. AU - Bendayan, D. AU - van Uitert, R. AU - Onnekink, C. AU - Pruijn, G.J.M. PY - 2010 UR - https://hdl.handle.net/2066/83945 TI - The anti-cyclic citrullinated peptide response in tuberculosis patients is not citrulline-dependent and sensitive to treatment SN - 1478-6354 IS - iss. 1 SP - R12 JF - Arthritis Research & Therapy VL - vol. 12 PS - 7 p. DO - https://doi.org/10.1186/ar2913 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/83945/83945.pdf?sequence=1 ER - TY - JOUR AU - Smits, P. AU - Mattijssen, S. AU - Morava, E. AU - Brand, M. van den AU - Brandt, F. van den AU - Wijburg, F. AU - Pruijn, G.J.M. AU - Smeitink, J.A.M. AU - Nijtmans, L.G.J. AU - Rodenburg, R.J.T. AU - Heuvel, L.P.W.J. van den PY - 2010 UR - https://hdl.handle.net/2066/83310 AB - Combined oxidative phosphorylation (OXPHOS) system deficiencies are a group of mitochondrial disorders that are associated with a range of clinical phenotypes and genetic defects. They occur in approximately 30% of all OXPHOS disorders and around 4% are combined complex I, III and IV deficiencies. In this study we present two mutations in the mitochondrial tRNA(Trp) (MT-TW) and tRNA(Arg) (MT-TR) genes, m.5556G>A and m.10450A>G, respectively, which were detected in two unrelated patients showing combined OXPHOS complex I, III and IV deficiencies and progressive multisystemic diseases. Both mitochondrial tRNA mutations were almost homoplasmic in fibroblasts and muscle tissue of the two patients and not present in controls. Patient fibroblasts showed a general mitochondrial translation defect. The mutations resulted in lowered steady-state levels and altered conformations of the tRNAs. Cybrid cell lines showed similar tRNA defects and impairment of OXPHOS complex assembly as patient fibroblasts. Our results show that these tRNA(Trp) and tRNA(Arg) mutations cause the combined OXPHOS deficiencies in the patients, adding to the still expanding group of pathogenic mitochondrial tRNA mutations. TI - Functional consequences of mitochondrial tRNA Trp and tRNA Arg mutations causing combined OXPHOS defects. EP - 329 SN - 1018-4813 IS - iss. 3 SP - 324 JF - European Journal of Human Genetics VL - vol. 18 N1 - 1 maart 2010 PS - 6 p. DO - https://doi.org/10.1038/ejhg.2009.169 ER - TY - JOUR AU - Kidane, A.H. AU - Heinrich, G. AU - Dirks, R.P.H. AU - de Ruyck, B.A. AU - Lubsen, N.H. AU - Roubos, E.W. AU - Jenks, B.G. PY - 2009 UR - https://hdl.handle.net/2066/75495 TI - Differential neuroendocrine expression of multiple brain-derived neurotrophic factor transcripts EP - 1368 SN - 0013-7227 IS - iss. 3 SP - 1361 JF - Endocrinology VL - vol. 150 PS - 9 p. DO - http://dx.doi.org/10.1210/en.2008-0993 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/75495/75495.pdf?sequence=1 ER - TY - JOUR AU - Hagemann, T.L. AU - Boelens, W.C. AU - Wawrousek, E.F. AU - Messing, A. PY - 2009 UR - https://hdl.handle.net/2066/75823 TI - Suppression of GFAP toxicity by alphaB-crystallin in mouse models of Alexander disease EP - 1199 SN - 0964-6906 IS - iss. 7 SP - 1190 JF - Human Molecular Genetics VL - vol. 18 PS - 10 p. DO - http://dx.doi.org/10.1093/hmg/ddp013 ER - TY - JOUR AU - Borghuis, A. AU - Groenendael, J.M. van AU - Madsen, O. AU - Ouborg, N.J. PY - 2009 UR - https://hdl.handle.net/2066/75995 TI - Phylogenetic analyses of the leaf beetle genus Galerucella: evidence for host switching at speciation? EP - 367 SN - 1055-7903 IS - iss. 2 SP - 361 JF - Molecular Phylogenetics and Evolution VL - vol. 53 PS - 7 p. DO - http://dx.doi.org/10.1016/j.ympev.2009.07.005 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Kox, M. AU - Maat-Schieman, M.L. AU - Veerhuis, R. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2009 UR - https://hdl.handle.net/2066/75841 TI - Small heat shock proteins associated with cerebral amyloid angiopathy of hereditary cerebral hemorrhage with amyloidosis (Dutch type) induce interleukin-6 secretion EP - 240 SN - 0197-4580 IS - iss. 2 SP - 229 JF - Neurobiology of Aging VL - vol. 30 PS - 13 p. DO - https://doi.org/10.1016/j.neurobiolaging.2007.06.001 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Kox, M. AU - Maat-Schieman, M.L. AU - Veerhuis, R. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2009 UR - https://hdl.handle.net/2066/81507 AB - In hereditary cerebral hemorrhage with amyloidosis of the Dutch type (HCHWA-D), severe cerebral amyloid angiopathy (CAA) is associated with an inflammatory reaction. Small heat shock proteins (sHsps) are molecular chaperones and association of HspB8 with CAA in HCHWA-D has been observed. The aims of this study were to investigate (1) if other sHsps are associated with the pathological lesions in HCHWA-D brains, (2) if the amyloid-beta protein (A beta) increases production of sHsps in cultured cerebral cells and (3) if sHsps are involved in the cerebral inflammatory processes in both Alzheimer's disease (AD) and HCHWA-D. We conclude that Hsp20, HspB8 and HspB2 are present in CAA in HCHWA-D, and that A beta did not affect cellular sHsps expression in cultured human brain pericytes and astrocytes. In addition, we demonstrated that Hsp20, HspB2 and HspB8 induced interleukin-6 production in cultured pericytes and astrocytes, which could be antagonized by dexamethasone, whereas other sHsps and A beta were inactive, suggesting that sHsps may be among the key mediators of the local inflammatory response associated with HCHWA-D and AD lesions. TI - Small heat shock proteins associated with cerebral amyloid angiopathy of hereditary cerebral hemorrhage with amyloidosis (Dutch type) induce interleukin-6 secretion. EP - 240 SN - 0197-4580 IS - iss. 2 SP - 229 JF - Neurobiology of Aging VL - vol. 30 DO - https://doi.org/10.1016/j.neurobiolaging.2007.06.001 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2009 UR - https://hdl.handle.net/2066/75459 TI - De vicieuze cirkel die leidt tot reumatoide artritis EP - B232-6 SN - 0028-2162 SP - B232-1 JF - Nederlands Tijdschrift voor Geneeskunde VL - vol. 153 PS - 7 p. ER - TY - JOUR AU - Engelsman, J. AU - Boros, S. AU - Dankers, P.Y. AU - Kamps, B. AU - Vree Egberts, W.T.M. AU - Bode, C.S. AU - Lane, L.A. AU - Aquilina, J.A. AU - Benesch, J.L. AU - Robinson, C.V. AU - Jong, W.W.W. de AU - Boelens, W.C. PY - 2009 UR - https://hdl.handle.net/2066/81177 AB - Various mammalian small heat-shock proteins (sHSPs) can interact with one another to form large polydisperse assemblies. In muscle cells, HSPB2/MKBP (myotonic dystrophy protein kinase-binding protein) and HSPB3 have been shown to form an independent complex. To date, the biochemical properties of this complex have not been thoroughly characterized. In this study, we show that recombinant HSPB2 and HSPB3 can be successfully purified from Escherichia coli cells co-expressing both proteins. Nanoelectrospray ionization mass spectrometry and sedimentation velocity analytical ultracentrifugation analysis showed that HSPB2/B3 forms a series of well defined hetero-oligomers, consisting of 4, 8, 12, 16, 20 and 24 subunits, each maintaining a strict 3:1 HSPB2/HSPB3 subunit ratio. These complexes are thermally stable up to 40 degrees C, as determined by far-UV circular dichroism spectroscopy. Surprisingly, HSPB2/B3 exerted a poor chaperone-like and thermoprotective activity, which is likely related to the low surface hydrophobicity, as revealed by its interaction with the hydrophobic probe 1-anilino-8-naphthalenesulfonic acid. Co-immunoprecipitation experiments demonstrated that the HSPB2/B3 oligomer cannot interact with HSP20, HSP27 or alphaB-crystallin, whereas the homomeric form of HSPB2, thus not in complex with HSPB3, could associate efficiently with HSP20. Taken altogether, this study provides evidence that, despite the high level of sequence homology within the sHSP family the biochemical properties of the HSPB2/B3 complex are distinctly different from those of other sHSPs, indicating that the HSPB2/B3 assembly is likely to possess cellular functions other than those of its family members. TI - The small heat-shock proteins HSPB2 and HSPB3 form well-defined heterooligomers in a unique 3 to 1 subunit ratio. EP - 1032 SN - 0022-2836 IS - iss. 5 SP - 1022 JF - Journal of Molecular Biology VL - vol. 393 DO - https://doi.org/10.1016/j.jmb.2009.08.052 ER - TY - JOUR AU - Reulen, S.W.A. AU - Baal, I. Van AU - Raats, J.M.H. AU - Merkx, M.A.W. PY - 2009 UR - https://hdl.handle.net/2066/75302 TI - Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester EP - 9 SN - 1472-6750 SP - 1 JF - BMC Biotechnology VL - vol. 9 PS - 9 p. DO - https://doi.org/10.1186/1472-6750-9-66 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/75302/75302.pdf?sequence=1 ER - TY - JOUR AU - Roodink, I. AU - Verrijp, K. AU - Raats, J.M.H. AU - Leenders, W.P.J. PY - 2009 UR - https://hdl.handle.net/2066/76003 TI - Plexin D1 is ubiquitously expressed on tumor vessels and tumor cells in solid malignancies SN - 1471-2407 JF - BMC Cancer VL - vol. 9 DO - https://doi.org/10.1186/1471-2407-9-297 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/76003/76003.pdf?sequence=1 ER - TY - JOUR AU - Bagneris, C. AU - Bateman, O.A. AU - Naylor, C.E. AU - Cronin, N. AU - Boelens, W.C. AU - Keep, N.H. AU - Slingsby, C PY - 2009 UR - https://hdl.handle.net/2066/75423 TI - Crystal structures of alpha-crystallin domain dimers of alphaB-crystallin and Hsp20 EP - 1252 SN - 1083-351X IS - iss. 5 SP - 1242 JF - Journal of Biological Chemistry VL - vol. 392 PS - 11 p. ER - TY - JOUR AU - Engelsman, J.P. den AU - Boros, S. AU - Dankers, P.Y. AU - Kamps, B. AU - Vree Egberts, W.T.M. AU - Bode, C.S. AU - Lane, L.A. AU - Aquilina, J.A. AU - Benesch, J.L. AU - Robinson, C.V. AU - Jong, W.W.W. de AU - Boelens, W.C. PY - 2009 UR - https://hdl.handle.net/2066/75686 TI - The small heat-shock proteins HSPB2 and HSPB3 form well-defined heterooligomers in a unique 3 to 1 subunit ratio SN - 1083-351X IS - iss. 5 JF - Journal of Biological Chemistry VL - vol. 393 ER - TY - JOUR AU - Kidane, A.H. AU - Heinrich, G. AU - Dirks, R.P.H. AU - Ruyck, B.A. de AU - Lubsen, N.H. AU - Roubos, E.W. AU - Jenks, B.G. PY - 2008 UR - https://hdl.handle.net/2066/72169 TI - Differential neuroendocrine expression of multiple brain-derived neurotrophic factor transcripts EP - 1368 SN - 0013-7227 IS - iss. 3 SP - 1361 JF - Endocrinology VL - vol. 150 PS - 8 p. DO - http://dx.doi.org/10.1210/en.2008-0993 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/72169/72169.pdf?sequence=1 ER - TY - JOUR AU - Schilders, G.W. AU - Pruijn, G.J.M. PY - 2008 UR - https://hdl.handle.net/2066/71969 TI - Biochemical studies of the mammalian exosome with intact cells EP - 226 SN - 0076-6879 SP - 211 JF - Methods in Enzymology . New York VL - vol. 448 PS - 6 p. DO - http://dx.doi.org/10.1016/S0076-6879(08)02611-6 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Beers, J.J.B.C. van AU - Pruijn, G.J.M. PY - 2008 UR - https://hdl.handle.net/2066/72017 TI - Anti-CCP antibody, a marker for the early detection of rheumatoid arthritis EP - 285 SN - 0077-8923 IS - iss. 1143 SP - 268 JF - Annals of the New York Academy of Sciences VL - vol. 2008 PS - 16 p. ER - TY - JOUR AU - Quraishe, S. AU - Asuni, A. AU - Boelens, W.C. AU - Connor, R. AU - Wyttenbach, A. PY - 2008 UR - https://hdl.handle.net/2066/72366 TI - Expression of the small heat shock protein family in the mouse CNS: Differential anatomical and biochemical compartmentalization EP - 491 SN - 0306-4522 IS - iss. 2 SP - 483 JF - Neuroscience VL - vol. 153 PS - 9 p. DO - http://dx.doi.org/10.1016/j.neuroscience.2008.01.058 ER - TY - JOUR AU - Venrooij, W.J.W. van PY - 2008 UR - https://hdl.handle.net/2066/72473 TI - The diagnostic accuracy of anti-citrulline antibody assessment in the diagnosis of patients suspected of rheumatoid arthritis by a general practitioner EP - 1244 SN - 0028-2162 IS - iss. 21 SP - 1243 JF - Nederlands Tijdschrift voor Geneeskunde VL - vol. 152 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2008 UR - https://hdl.handle.net/2066/72622 TI - An important step towards completing the rheumatoid arthritis cycle EP - 120 SN - 1478-6354 IS - iss. 5 SP - 117 JF - Arthritis Research & Therapy VL - vol. 10 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/72622/72622.pdf?sequence=1 ER - TY - JOUR AU - Roodink, I. AU - Kats, G. AU - Kempen, L.T.C. Van AU - Grunberg, M. AU - Maass, C.N. AU - Verrijp, K. AU - Raats, J.M.H. AU - Leenders, W.P.J. PY - 2008 UR - https://hdl.handle.net/2066/121071 TI - Semaphorin 3E expression correlates inversely with Plexin D1 during tumor progression EP - 1881 SN - 0002-9440 IS - iss. 6 SP - 1873 JF - American Journal of Pathology VL - vol. 173 PS - 9 p. DO - http://dx.doi.org/10.2353/ajpath.2008.080136 ER - TY - JOUR AU - Noordman, Y.E. AU - Augustus, E.D. AU - Schepens, J.T.G. AU - Chirivi, R.G.S. AU - Rios, P. AU - Pulido, R. AU - Hendriks, W.J.A.J. PY - 2008 UR - https://hdl.handle.net/2066/70701 AB - Dimerisation of receptor-type protein tyrosine phosphatases (RPTPs) represents an appealing mechanism to regulate their enzymatic activity. Studies thus far mostly concern the dimerisation behaviour of RPTPs possessing two tandemly oriented catalytic PTP domains. Mouse gene Ptprr encodes four different protein isoforms (i.e. PTPBR7, PTP-SL and PTPPBSgamma-42/37) that contain a single PTP domain. Using selective membrane permeabilisation we here demonstrate that PTP-SL, like PTPBR7, is a single membrane-spanning RPTP. Furthermore, these two receptor-type PTPs constitutively formed homo- and hetero-meric complexes as witnessed in chemical cross-linking and co-immunoprecipitation experiments, in sharp contrast to the cytosolic PTPPBSgamma-42 and PTPPBSgamma-37 PTPRR isoforms. This multimerisation occurs independently of the PTP domain and requires the transmembrane domain and/or the proximal hydrophobic region. Using overexpression of a PTPBR7 mutant that essentially lacks the intracellular PTP domain-containing segment, a monomer-mimicking state was forced upon full-length PTPBR7 immunoprecipitates. This resulted in a significant increase in the enzymatic activity of the PTPRR PTP domain, which strengthens the notion that multimerisation represents a general mechanism to tone down RPTP catalytic activity. TI - Multimerisation of receptor-type protein tyrosine phosphatases PTPBR7 and PTP-SL attenuates enzymatic activity. EP - 286 SN - 0167-4889 IS - iss. 2 SP - 275 JF - Biochimica et Biophysica Acta. Molecular Cell Research VL - vol. 1783 DO - http://dx.doi.org/10.1016/j.bbamcr.2007.10.023 ER - TY - JOUR AU - Franck, E. AU - Hulsen, T. AU - Huynen, M.A. AU - Jong, W.W.W. de AU - Lubsen, N.H. AU - Madsen, O. PY - 2008 UR - https://hdl.handle.net/2066/70352 AB - The orientation of closely linked genes in mammalian genomes is not random: there are more head-to-head (h2h) gene pairs than expected. To understand the origin of this enrichment in h2h gene pairs, we have analyzed the phylogenetic distribution of gene pairs separated by less than 600 bp of intergenic DNA (gene duos). We show here that a lack of head-to-tail (h2t) gene duos is an even more distinctive characteristic of mammalian genomes, with the platypus genome as the only exception. In nonmammalian vertebrate and in nonvertebrate genomes, the frequency of h2h, h2t, and tail-to-tail (t2t) gene duos is close to random. In tetrapod genomes, the h2t and t2t gene duos are more likely to be part of a larger gene cluster of closely spaced genes than h2h gene duos; in fish and urochordate genomes, the reverse is seen. In human and mouse tissues, the expression profiles of gene duos were skewed toward positive coexpression, irrespective of orientation. The organization of orthologs of both members of about 40% of the human gene duos could be traced in other species, enabling a prediction of the organization at the branch points of gnathostomes, tetrapods, amniotes, and euarchontoglires. The accumulation of h2h gene duos started in tetrapods, whereas that of h2t and t2t gene duos only started in amniotes. The apparent lack of evolutionary conservation of h2t and t2t gene duos relative to that of h2h gene duos is thus a result of their relatively late origin in the lineage leading to mammals; we show that once they are formed h2t and t2t gene duos are as stable as h2h gene duos. TI - Evolution of closely linked gene pairs in vertebrate genomes. EP - 1921 SN - 0737-4038 IS - iss. 9 SP - 1909 JF - Molecular Biology and Evolution VL - vol. 25 PS - 12 p. DO - http://dx.doi.org/10.1093/molbev/msn136 ER - TY - JOUR AU - Boros, S. AU - Wilmarth, P.A. AU - Kamps, B. AU - Jong, W.W.W. de AU - Bloemendal, H. AU - Lampi, K. AU - Boelens, W.C. PY - 2008 UR - https://hdl.handle.net/2066/70612 AB - Tissue transglutaminase (tTG) is a Ca(2+)-dependent enzyme catalyzing the formation of covalent crosslinks between peptide-bound glutamine and lysine residues. Lens crystallins, including alphaB-crystallin and several beta-crystallins, are in vitro substrates for tTG. In both human and bovine fetal lens extracts treated with commercially available guinea pig liver tTG we detected the formation of high molecular weight (HMW) aggregates containing crosslinked betaB(2)- and betaA(3)-crystallin. More interestingly, 2D-gel electrophoresis combined with mass spectrometry analysis revealed that glutamines present in the N-terminal arms of betaB(2)- and betaB(3)-crystallins deamidate readily in the presence of tTG. We found that both tTG-catalyzed crosslinking and deamidation disrupt the beta-crystallin complex, suggesting that these tTG-catalyzed modifications can influence the macromolecular assembly of lens crystallins. These data together suggest that tTG can contribute to the age-related deamidation of glutamine residues of lens crystallins. TI - Tissue transglutaminase catalyzes the deamidation of glutamines in lens betaB(2)- and betaB(3)-crystallins. EP - 393 SN - 0014-4835 IS - iss. 2 SP - 383 JF - Experimental Eye Research VL - vol. 86 DO - https://doi.org/10.1016/j.exer.2007.11.011 ER - TY - JOUR AU - Verpoort, K.N. AU - Ioan, A. AU - Pruijn, G.J.M. AU - Toes, R.E. PY - 2008 UR - https://hdl.handle.net/2066/72259 TI - Reply EP - 3278 SN - 0004-3591 IS - iss. 10 SP - 3277 JF - Arthritis and Rheumatism VL - vol. 58 PS - 2 p. ER - TY - JOUR AU - Takahashi, N. AU - Kilsdonk, J.W.J. van AU - Ostendorf, B. AU - Smeets, R. AU - Bruggeman, S.W. AU - Alonso, A. AU - Loo, F.A.J. van de AU - Schneider, M. AU - Berg, W.B. van den AU - Swart, G.W.M. PY - 2008 UR - https://hdl.handle.net/2066/70553 TI - Tumor marker nucleoporin 88 kDa regulates nucleocytoplasmic transport of NF-kappaB. EP - 430 SN - 0006-291X IS - iss. 3 SP - 424 JF - Biochemical and Biophysical Research Communications VL - vol. 374 PS - 7 p. DO - https://doi.org/10.1016/j.bbrc.2008.06.128 ER - TY - JOUR AU - Franck, E. AU - Hulsen, T. AU - Huynen, M.A. AU - Jong, W.W.W. de AU - Lubsen, N.H. AU - Madsen, O. PY - 2008 UR - https://hdl.handle.net/2066/70351 TI - Evolution of closely linked gene pairs in vertebrate genomes EP - 1921 SN - 0737-4038 IS - iss. 9 SP - 1909 JF - Molecular Biology and Evolution VL - vol. 25 PS - 13 p. DO - https://doi.org/10.1093/molbev/msn136 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Zendman, A.J.W. PY - 2008 UR - https://hdl.handle.net/2066/65997 TI - Anti-CCP2 antibodies: An overview and perspective of the diagnostic abilities of this serological marker for early rheumatoid arthritis EP - 39 SN - 1080-0549 IS - iss. 1 SP - 36 JF - Clinical Reviews in Allergy and Immunology VL - vol. 34 PS - 4 p. DO - https://doi.org/10.1007/s12016-007-8029-y L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/65997/65997.pdf?sequence=1 ER - TY - JOUR AU - Beusink, J.B. AU - Lokate, A.M.C. AU - Besselink, G.A.J. AU - Pruijn, G.J.M. AU - Schasfoort, R.B.M. PY - 2008 UR - https://hdl.handle.net/2066/66004 TI - Angle-scanning SPR imaging for detection of biomolecular interactions on microarrays EP - 844 SN - 0956-5663 IS - iss. 6 SP - 839 JF - Biosensors and Bioelectronics VL - vol. 23 PS - 5 p. DO - https://doi.org/10.1016/j.bios.2007.08.025 ER - TY - JOUR AU - Kilsdonk, J.W.J. van AU - Wilting, R.H. AU - Bergers, A.M.G. AU - Muijen, G.N.P. Van AU - Schalkwijk, J. AU - Kempen, L.C.L.T. van AU - Swart, G.W.M. PY - 2008 UR - https://hdl.handle.net/2066/69986 TI - Attenuation of melanoma invasion by a secreted variant of activated leukocyte cell adhesion molecule EP - 3679 SN - 0008-5472 IS - iss. 10 SP - 3671 JF - Cancer Research VL - vol. 68 PS - 9 p. DO - https://doi.org/10.1158/0008-5472.CAN-07-5767 ER - TY - JOUR AU - Kilsdonk, J.W.J. van AU - Wilting, R.H. AU - Bergers, M. AU - Muijen, G.N.P. van AU - Schalkwijk, J. AU - Kempen, L.C.L.T. van AU - Swart, G.W.M. PY - 2008 UR - https://hdl.handle.net/2066/69993 AB - Activated leukocyte cell adhesion molecule (ALCAM/CD166/MEMD), a marker of various cancers and mesenchymal stem cells, is involved in melanoma metastasis. We have exploited a secreted NH(2)-terminal fragment, sALCAM, to test the hypothesis that ALCAM coordinates tissue growth and cell migration. Overexpression of sALCAM in metastatic melanoma cells disturbed clustering of endogenous ALCAM and inhibited activation of matrix metalloproteinase-2 (MMP-2). Exposure of HT1080 fibrosarcoma cells to sALCAM similarly inhibited MMP-2, suggesting a broader effect on ALCAM-positive tumor cells. In contrast to the previously reported, promotive effects of an NH(2)-terminally truncated, transmembrane variant (DeltaN-ALCAM), sALCAM impaired the migratory capacity of transfected cells in vitro, reduced basement membrane penetration in reconstituted human skin equivalents, and diminished metastatic capacity in nude mice. Remarkably, L1 neuronal cell adhesion molecule (L1CAM/CD171), another progression marker of several cancers including melanoma, was suppressed upon sALCAM overexpression but was up-regulated by DeltaN-ALCAM. The partially overlapping and opposite effects induced by alternative strategies targeting ALCAM functions collectively attribute an integrative role to ALCAM in orchestrating cell adhesion, growth, invasion, and proteolysis in the tumor tissue microenvironment and disclose a therapeutic potential for sALCAM. TI - Attenuation of melanoma invasion by a secreted variant of activated leukocyte cell adhesion molecule. EP - 3679 SN - 0008-5472 IS - iss. 10 SP - 3671 JF - Cancer Research VL - vol. 68 PS - 9 p. DO - https://doi.org/10.1158/0008-5472.CAN-07-5767 ER - TY - JOUR AU - Christis, C. AU - Lubsen, N.H. AU - Braakman, I. PY - 2008 UR - https://hdl.handle.net/2066/71339 TI - Protein folding includes oligomerization - examples from the endoplasmic reticulum and cytosol EP - 4727 SN - 1742-464X IS - iss. 19 SP - 4700 JF - FEBS Journal VL - vol. 275 DO - https://doi.org/10.1111/j.1742-4658.2008.06590.x ER - TY - JOUR AU - Poux, C.M. AU - Madsen, O. AU - Glos, J. AU - Jong, W.W.W. de AU - Vences, M. PY - 2008 UR - https://hdl.handle.net/2066/72732 TI - Molecular phylogeny and divergence times of Malagasy tenrecs: Influence of data partitioning and taxon sampling on dating analyses SN - 1471-2148 JF - BMC Evolutionary Biology VL - vol. 8 PS - 16 p. DO - https://doi.org/10.1186/1471-2148-8-102 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/72732/72732.pdf?sequence=1 ER - TY - JOUR AU - Boros, S. AU - Wilmarth, P.A. AU - Kamps, B. AU - Jong, W.W.W. de AU - Bloemendal, H. AU - Lampi, K. AU - Boelens, W.C. PY - 2008 UR - https://hdl.handle.net/2066/72436 TI - Tissue transglutaminase catalyzes the deamidation of glutamines in lens betaB2- and betaB3-crystallins EP - 393 SN - 0014-4835 IS - iss. 2 SP - 383 JF - Experimental Eye Research VL - vol. 86 PS - 11 p. DO - https://doi.org/10.1016/j.exer.2007.11.011 ER - TY - JOUR AU - Welting, T.J.M. AU - Mattijssen, S. AU - Peters, F.M. AU - Doorn, N.L. van AU - Dekkers, L. AU - Venrooij, W.J.W. van AU - Heus, H.A. AU - Bonafe, L. AU - Pruijn, G.J.M. PY - 2008 UR - https://hdl.handle.net/2066/71925 TI - Cartilage-hair hypoplasia-associated mutations in the RNase MRP P3 domain affect RNA folding and ribonucleoprotein assembly EP - 466 SN - 0006-3002 IS - iss. 3 SP - 455 JF - Biochimica et Biophysica Acta VL - vol. 1783 PS - 12 p. ER - TY - JOUR AU - Hof, D. AU - Hoeke, M.O. AU - Raats, J.M.H. PY - 2008 UR - https://hdl.handle.net/2066/72764 TI - Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens EP - 377 SN - 0009-9104 IS - iss. 2 SP - 367 JF - Clinical and Experimental Immunology VL - vol. 151 PS - 11 p. DO - https://doi.org/10.1111/j.1365-2249.2007.03569.x ER - TY - JOUR AU - Raijmakers, R. AU - Zendman, A.J.W. AU - Egberts, W.T.M. Vree AU - Vossenaar, E.R. AU - Raats, J.M.H. AU - Soede-Huijbregts, C. AU - Rutjes, F.P.J.T. AU - Veelen, P.A. van AU - Drijfhout, J.W. AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/34477 TI - Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro EP - 1129 SN - 0022-2836 IS - iss. 4 SP - 1118 JF - Journal of Molecular Biology VL - vol. 367 DO - http://dx.doi.org/10.1016/j.jmb.2007.01.054 ER - TY - JOUR AU - Mahler, M. AU - Raijmakers, R. PY - 2007 UR - https://hdl.handle.net/2066/35278 TI - Novel aspects of autoantibodies to the PM/Scl complex: clinical, genetic and diagnostic insights EP - 437 SN - 1568-9972 IS - iss. 7 SP - 432 JF - Autoimmunity Reviews VL - vol. 6 DO - http://dx.doi.org/10.1016/j.autrev.2007.01.013 ER - TY - JOUR AU - Kessenbrock, K. AU - Raijmakers, R. AU - Fritzler, M.J. AU - Mahler, M. PY - 2007 UR - https://hdl.handle.net/2066/36535 TI - Synthetic peptides: the future of patient management in systemic rheumatic diseases? EP - 2838 SN - 0929-8673 IS - iss. 26 SP - 2831 JF - Current Medicinal Chemistry VL - vol. 14 DO - http://dx.doi.org/10.2174/092986707782360150 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Kox, M. AU - Maat-Schieman, M.L. AU - Veerhuis, R. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2007 UR - https://hdl.handle.net/2066/36466 TI - Small heat shock proteins associated with cerebral amyloid angiopathy of hereditary cerebral hemorrhage with amyloidosis (Dutch type) induce interleukin-6 secretion EP - 240 SN - 0197-4580 IS - iss. 2 SP - 229 JF - Neurobiology of Aging VL - vol. 30 DO - https://doi.org/10.1016/j.neurobiolaging.2007.06.001 ER - TY - JOUR AU - Vannini, A. AU - Cheung, K. AU - Fusconi, M. AU - Stammen-Vogelzangs, J. AU - Drenth, J.P.H. AU - Dall'aglio, A.C. AU - Bianchi, F.B. AU - Bakker-Jonges, L.E. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. AU - Zendman, A.J.W. PY - 2007 UR - https://hdl.handle.net/2066/187121 TI - Anti-cyclic citrullinated peptide positivity in non-rheumatoid arthritis disease samples: citrulline-dependent or not? EP - 516 SN - 0003-4967 IS - iss. 4 SP - 511 JF - Annals of the Rheumatic Diseases VL - vol. 66 ER - TY - JOUR AU - Verpoort, K.N. AU - Cheung, K. AU - Ioan-Facsinay, A. AU - Helm-van Mil, A.H. van der AU - Vries-Bouwstra, J.K. de AU - Allaart, C.F. AU - Drijfhout, J.W. AU - Vries, R.R.P. de AU - Breedveld, F.C. AU - Huizinga, T.W.J. AU - Pruijn, G.J.M. AU - Toes, R.E. PY - 2007 UR - https://hdl.handle.net/2066/34781 TI - Fine specificity of the anti-citrullinated protein antibody response is influenced by the shared epitope alleles EP - 3952 SN - 0004-3591 IS - iss. 12 SP - 3949 JF - Arthritis and Rheumatism VL - vol. 56 DO - https://doi.org/10.1002/art.23127 ER - TY - JOUR AU - Vannini, A. AU - Cheung, K. AU - Fusconi, M. AU - Stammen-Vogelzangs, J. AU - Drenth, J.P.H. AU - Dall'aglio, A.C. AU - Bianchi, F.B. AU - Bakker-Jonges, L.E. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. AU - Zendman, A.J.W. PY - 2007 UR - https://hdl.handle.net/2066/35130 AB - BACKGROUND: Antibodies directed against citrullinated proteins (eg anti-cyclic citrullinated peptide (CCP)) have excellent diagnostic and good prognostic potential for rheumatoid arthritis. Type 1 autoimmune hepatitis (AIH-1) is a chronic liver disease characterised by a variety of serum autoantibodies. Recently, in a large group of patients with AIH-1 without clear rheumatoid arthritis overlap, a relatively high percentage (9%) of anti-CCP2 positivity was scored. OBJECTIVES: To characterise the citrulline-dependence of the observed anti-CCP2 positivity in AIH-1 sera as well as in other groups of patients without rheumatoid arthritis (mainly rheumatic diseases). METHODS: Serum samples of 57 patients with AIH-1 and 66 patients without rheumatoid arthritis, most of them reported as anti-CCP positive, were tested for citrulline-specific reactivity with a second generation anti-CCP kit, with the citrullinated and the corresponding non-citrullinated (arginine-containing) antigen. A subset of AIH-1 sera was also tested with a CCP1 ELISA (and arginine control). RESULTS: The anti-CCP2 reactivity of most non-rheumatoid arthritis rheumatic diseases samples (87-93%) was citrulline-specific, whereas a relatively high percentage of AIH-1 samples (42-50%) turned out to be reactive in a citrulline-independent manner. The use of citrullinated and non-citrullinated CCP1 peptides confirmed a high occurrence of citrulline-independent reactivity in AIH-1 samples. CONCLUSIONS: In rheumatoid arthritis and most non-rheumatoid arthritis rheumatologic disease sera, anti-CCP positivity is citrulline-dependent. However in some patients, particularly patients with AIH-1, citrulline-independent reactivity in the anti-CCP2 test can occur. A positive CCP test in a non-rheumatic disease (eg liver disease) should therefore be interpreted with care, and preferably followed by a control ELISA with a non-citrullinated antigen. TI - Anti-cyclic citrullinated peptide positivity in non-rheumatoid arthritis disease samples: citrulline-dependent or not? EP - 516 SN - 0003-4967 IS - iss. 4 SP - 511 JF - Annals of the Rheumatic Diseases VL - vol. 66 ER - TY - JOUR AU - Muris, J.J. AU - Ylstra, B. AU - Cillessen, S.A. AU - Ossenkoppele, G.J. AU - Kluin-Nelemans, J.C. AU - Eijk, P.P. AU - Nota, B. AU - Tijssen, M. AU - Boer, W.P. de AU - Wiel, M. van de AU - IJssel, P.R.L.A. van den AU - Jansen, P. AU - Bruin, P.C. de AU - Krieken, J.H.J.M. van AU - Meijer, G.A. AU - Meijer, C.J. AU - Oudejans, J.J. PY - 2007 UR - https://hdl.handle.net/2066/36362 AB - Intrinsic resistance of lymphoma cells to apoptosis is a probable mechanism causing chemotherapy resistance and eventual fatal outcome in patients with diffuse large B cell lymphomas (DLBCL). We investigated whether microarray expression profiling of apoptosis related genes predicts clinical outcome in 46 patients with primary nodal DLBCL. Unsupervised cluster analysis using genes involved in apoptosis (n = 246) resulted in three separate DLBCL groups partly overlapping with germinal centre B-lymphocytes versus activated B-cells like phenotype. One group with poor clinical outcome was characterised by high expression levels of pro-and anti-apoptotic genes involved in the intrinsic apoptosis pathway. A second group, also with poor clinical outcome, was characterised by high levels of apoptosis inducing cytotoxic effector genes, possibly reflecting a cellular cytotoxic immune response. The third group showing a favourable outcome was characterised by low expression levels of genes characteristic for both other groups. Our results suggest that chemotherapy refractory DLBCL are characterised either by an intense cellular cytotoxic immune response or by constitutive activation of the intrinsic mediated apoptosis pathway with concomitant downstream inhibition of this apoptosis pathway. Consequently, strategies neutralising the function of apoptosis-inhibiting proteins might be effective as alternative treatment modality in part of chemotherapy refractory DLBCL. TI - Profiling of apoptosis genes allows for clinical stratification of primary nodal diffuse large B-cell lymphomas. EP - 47 SN - 0007-1048 IS - iss. 1 SP - 38 JF - British Journal of Haematology VL - vol. 136 DO - https://doi.org/10.1111/j.1365-2141.2006.06375.x ER - TY - JOUR AU - Dijk, E.L. van AU - Schilders, G.W. AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/34686 TI - Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways EP - 1035 SN - 1355-8382 IS - iss. 7 SP - 1027 JF - Rna : a Publication of the Rna Society VL - vol. 13 DO - https://doi.org/10.1261/rna.575107 ER - TY - JOUR AU - Schilders, G.W. AU - Dijk, E.L. van AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/35046 TI - C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing EP - 2572 SN - 0305-1048 IS - iss. 8 SP - 2564 JF - Nucleic Acids Research VL - vol. 35 DO - https://doi.org/10.1093/nar/gkm082 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35046/35046.pdf?sequence=1 ER - TY - JOUR AU - Chirivi, R.G.S. AU - Noordman, Y.E. AU - Zee, C.E.E.M. van der AU - Hendriks, W.J.A.J. PY - 2007 UR - https://hdl.handle.net/2066/35148 AB - The neuronal protein tyrosine phosphatases encoded by mouse gene Ptprr (PTPBR7, PTP-SL, PTPPBSgamma-42 and PTPPBSgamma-37) have been implicated in mitogen-activated protein (MAP) kinase deactivation on the basis of transfection experiments. To determine their physiological role in vivo, we generated mice that lack all PTPRR isoforms. Ptprr-/- mice were viable and fertile, and not different from wildtype littermates regarding general physiology or explorative behaviour. Highest PTPRR protein levels are in cerebellum Purkinje cells, but no overt effects of PTPRR deficiency on brain morphology, Purkinje cell number or dendritic branching were detected. However, MAP kinase phosphorylation levels were significantly altered in the PTPRR-deficient cerebellum and cerebrum homogenates. Most notably, increased phospho-ERK1/2 immunostaining density was observed in the basal portion and axon hillock of Ptprr-/- Purkinje cells. Concomitantly, Ptprr-/- mice displayed ataxia characterized by defects in fine motor coordination and balance skills. Collectively, these results establish the PTPRR proteins as physiological regulators of MAP kinase signalling cascades in neuronal tissue and demonstrate their involvement in cerebellum motor function. TI - Altered MAP kinase phosphorylation and impaired motor coordination in PTPRR deficient mice. EP - 840 SN - 0022-3042 IS - iss. 3 SP - 829 JF - Journal of Neurochemistry VL - vol. 101 DO - https://doi.org/10.1111/j.1471-4159.2006.04398.x ER - TY - JOUR AU - Lokate, A.M.C. AU - Beusink, J.B. AU - Besselink, G.A. AU - Pruijn, G.J.M. AU - Schasfoort, R.B.M. PY - 2007 UR - https://hdl.handle.net/2066/35079 TI - Biomolecular interaction monitoring of autoantibodies by scanning surface plasmon resonance microarray imaging EP - 14018 SN - 0002-7863 IS - iss. 45 SP - 14013 JF - Journal of the American Chemical Society VL - vol. 129 DO - https://doi.org/10.1021/ja075103x ER - TY - JOUR AU - Beusink, J.B. AU - Lokate, A.M.C. AU - Besselink, G.A.J. AU - Pruijn, G.J.M. AU - Schasfoort, R.B.M. PY - 2007 UR - https://hdl.handle.net/2066/35124 TI - Angle-scanning SPR imaging for detection of biomolecular interactions on microarrays EP - 844 SN - 0956-5663 IS - iss. 6 SP - 839 JF - Biosensors and Bioelectronics VL - vol. Epub 2007 ER - TY - JOUR AU - Ahrman, E. AU - Gustavsson, N. AU - Hultschig, C. AU - Boelens, W.C. AU - Emanuelsson, C.S. PY - 2007 UR - https://hdl.handle.net/2066/36467 TI - Small heat shock proteins prevent aggregation of citrate synthase and bind to the N-terminal region which is absent in thermostable forms of citrate synthase EP - 666 SN - 1431-0651 IS - iss. 5 SP - 659 JF - Extremophiles VL - vol. 11 DO - https://doi.org/10.1007/s00792-007-0080-3 ER - TY - JOUR AU - Schilders, G.W. AU - Raijmakers, R. AU - Malmegrim, K.C. AU - Walle, L. Vande AU - Saelens, X. AU - Vree Egberts, W.T.M. AU - Venrooij, W.J.W. van AU - Vandenabeele, P. AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/35021 TI - Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis EP - R12-9 SN - 1478-6354 IS - iss. 1 SP - R12 JF - Arthritis Research & Therapy VL - vol. 9 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35021/35021.pdf?sequence=1 ER - TY - JOUR AU - Schilders, G.W. AU - Vree Egberts, W.T.M. AU - Raijmakers, R. AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/35047 TI - C1D is a major autoantibody target in patients with the polymyositis-scleroderma overlap syndrome EP - 2454 SN - 0004-3591 IS - iss. 7 SP - 2449 JF - Arthritis and Rheumatism VL - vol. 56 DO - https://doi.org/10.1002/art.22710 ER - TY - JOUR AU - Zendman, A.J.W. AU - Raijmakers, R. AU - Nijenhuis, S. AU - Vossenaar, E.R. AU - Tillaart, M. van den AU - Chirivi, R.G.S. AU - Raats, J.M.H. AU - Venrooij, W.J.W. van AU - Drijfhout, J.W. AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/35240 TI - ABAP: antibody-based assay for peptidylarginine deiminase activity EP - 240 SN - 0003-2697 IS - iss. 2 SP - 232 JF - Analytical Biochemistry VL - vol. 369 DO - http://dx.doi.org/10.1016/j.ab.2007.07.009 ER - TY - JOUR AU - Hof, D. AU - Hoeke, M.O. AU - Raats, J.M.H. PY - 2007 UR - https://hdl.handle.net/2066/36259 TI - Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens EP - 377 SN - 0009-9104 IS - iss. 2 SP - 367 JF - Clinical and Experimental Immunology VL - vol. 151 DO - https://doi.org/10.1111/j.1365-2249.2007.03569.x ER - TY - JOUR AU - Welting, T.J.M. AU - Peters, F.M.A. AU - Hensen, S.M.M. AU - Doorn, N.L. van AU - Kikkert, B.J. AU - Raats, J.M.H. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/34671 TI - Heterodimerization regulates RNase MRP/RNase P association, localization, and expression of Rpp20 and Rpp25 EP - 75 SN - 1355-8382 IS - iss. 1 SP - 65 JF - Rna : a Publication of the Rna Society VL - vol. 13 DO - https://doi.org/10.1261/rna.237807 ER - TY - JOUR AU - Nakashima, E. AU - Tran, J.R. AU - Welting, T.J.M. AU - Pruijn, G.J.M. AU - Hirose, Y. AU - Nishimura, G. AU - Ohashi, H. AU - Schurman, S.H. AU - Cheng, J. AU - Candotti, F. AU - Nagaraja, R. AU - Ikegawa, S. AU - Schlessinger, D. PY - 2007 UR - https://hdl.handle.net/2066/35019 TI - Cartilage hair hypoplasia mutations that lead to RMRP promoter inefficiency or RNA transcript instability EP - 2681 SN - 1552-4825 IS - iss. 22 SP - 2675 JF - American Journal of Medical Genetics. Part A VL - vol. 143 ER - TY - JOUR AU - Boros, S. AU - Wilmarth, P.A. AU - Kamps, B. AU - Jong, W.W.W. de AU - Bloemendal, H. AU - Lampi, K. AU - Boelens, W.C. PY - 2007 UR - https://hdl.handle.net/2066/36629 TI - Tissue transglutaminase catalyzes the deamidation of glutamines in lens betaB2- and betaB3-crystallins EP - 393 SN - 0014-4835 IS - iss. 2 SP - 383 JF - Experimental Eye Research VL - vol. 86 DO - https://doi.org/10.1016/j.exer.2007.11.011 ER - TY - JOUR AU - Welting, T.J.M. AU - Mattijssen, S. AU - Peters, F.M. AU - Doorn, N.L. van AU - Dekkers, L. AU - Venrooij, W.J.W. van AU - Heus, H.A. AU - Bonafe, L. AU - Pruijn, G.J.M. PY - 2007 UR - https://hdl.handle.net/2066/35020 AB - Cartilage-hair hypoplasia (CHH) is caused by mutations in the gene encoding the RNA component of RNase MRP. Currently it is unknown how these mutations affect the function of this endoribonuclease. In this study we investigated the effect of mutations in the P3 domain on protein binding and RNA folding. Our data demonstrate that a number of P3 nucleotide substitutions reduced the efficiency of its interaction with Rpp25 and Rpp20, two protein subunits binding as a heterodimer to this domain. The CHH-associated 40G>A substitution, as well as the replacement of residue 47, almost completely abrogated Rpp25 and Rpp20 binding in different assays. Also other CHH-associated P3 mutations reduced the efficiency by which the RNase MRP RNA is bound by Rpp25-Rpp20. These data demonstrate that the most important residues for binding of the Rpp25-Rpp20 dimer reside in the apical stem-loop of the P3 domain. Structural analyses by NMR not only showed that this loop may adopt a pseudo-triloop structure, but also demonstrated that the 40G>A substitution alters the folding of this part of the P3 domain. Our data are the first to provide insight into the molecular mechanism by which CHH-associated mutations affect the function of RNase MRP. TI - Cartilage-hair hypoplasia-associated mutations in the RNase MRP P3 domain affect RNA folding and ribonucleoprotein assembly EP - 466 SN - 0006-3002 IS - iss. 3 SP - 455 JF - Biochimica et Biophysica Acta VL - vol. 1783 ER - TY - JOUR AU - Malecaze, F. AU - Lubsen, N.H. AU - Serre, B. AU - Decha, A. AU - Duboue, M. AU - Penary, M. AU - Berg, D. Van den AU - Arnaud, J.D. AU - Titeux, M. AU - Kremer, E.J. AU - Couderc, B. PY - 2006 UR - https://hdl.handle.net/2066/35727 TI - Lens cell targetting for gene therapy of prevention of posterior capsule ication EP - 1429 SN - 0969-7128 IS - iss. 19 SP - 1422 JF - Gene Therapy VL - vol. 13 ER - TY - JOUR AU - Marin Vinader, L. AU - Shin, C. AU - Onnekink, C. AU - Manley, J.L. AU - Lubsen, N.H. PY - 2006 UR - https://hdl.handle.net/2066/35783 TI - Hsp27 enhances recovery of splicing as well as rephosphorylation of SRp38 after hock EP - 894 SN - 1059-1524 IS - iss. 2 SP - 886 JF - Molecular Biology of the Cell VL - vol. 17 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35783/35783.pdf?sequence=1 ER - TY - JOUR AU - Raijmakers, R. AU - Vogelzangs, J.H.P. AU - Raats, J.M.H. AU - Panzenbeck, M. AU - Corby, M. AU - Jiang, H. AU - Thibodeau, M. AU - Haynes, N. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. AU - Werneburg, B. PY - 2006 UR - https://hdl.handle.net/2066/35888 TI - Experimental autoimmune encephalomyelitis induction in peptidylarginine ase 2 knockout mice EP - 226 SN - 0021-9967 IS - iss. 2 SP - 217 JF - Journal of Comparative Neurology VL - vol. 498 ER - TY - JOUR AU - David, J.C. AU - Boelens, W.C. AU - Grongnet, J.F. PY - 2006 UR - https://hdl.handle.net/2066/34888 TI - Up-regulation of heat shock protein HSP 20 in the hippocampus as an early response to hypoxia of the newborn EP - 581 SN - 0022-3042 IS - iss. 2 SP - 570 JF - Journal of Neurochemistry VL - vol. 99 DO - http://dx.doi.org/10.1111/j.1471-4159.2006.04071.x ER - TY - JOUR AU - Smeets, B. AU - Steenbergen, M.L.M. AU - Dijkman, H.B.P.M. AU - Verrijp, K. AU - Loeke, N. te AU - Aten, J. AU - Steenbergen, E. AU - Wetzels, J.F.M. PY - 2006 UR - https://hdl.handle.net/2066/36095 AB - BACKGROUND: Thy-1.1 transgenic mice develop hypercellular focal and segmental glomerulosclerosis (FSGS) lesions that mimic human collapsing FSGS, in 7 days after injection with anti-Thy-1.1 antibodies. These lesions consist of proliferating parietal epithelial cells (PECs). We questioned whether the angiotensin converting enzyme inhibitor (ACE), captopril, could prevent the development of FSGS and if protection is related to the timing of drug administration. METHODS: First, we compared the effect of captopril treatment with angiotensin II-(ANGII) independent antihypertensive therapy (triple therapy). Second, we tested the effects of captopril administered over four different time intervals: days -7 to 0 (Ca-7>0), days -7 to 7 (Ca-7>7), days 0-7 (Ca0>7) and days 3-7 (Ca3>7) (day 0 being the day of injection of the antibody). Results : In anti-Thy-1.1 injected control (C) mice we observed dedifferentiation and activation of podocytes, reflected by loss of ASD33 and increased expression of desmin, followed by a marked accumulation of PECs forming hypercellular lesions. PECs showed an increased expression of connective tissue growth factor (CTGF). Triple therapy or captorpil pre-treatment (Ca-7>0) had no significant effect on albuminuria or FSGS. In contrast, Ca0>7 and Ca3>7 treatment significantly lowered albuminuria and attenuated development of FSGS. The latter two treatments attenuated loss of ASD33 expression by podocytes but could not prevent increased desmin expression. In addition, these treatments reduced CTGF expression by PECs and prevented PEC proliferation. CONCLUSIONS: ACE inhibition, but not triple therapy, prevents the development of FSGS, suggesting an important role for ANGII. ACE inhibition has a protective effect even when started 3 days after the initial podocyte insult, which is probably related to the ability of ACE-inhibition to block PEC activation and proliferation. TI - Angiotensin converting enzyme inhibition prevents development of collapsing focal segmental glomerulosclerosis in Thy-1.1 transgenic mice. EP - 3097 SN - 0931-0509 IS - iss. 11 SP - 3087 JF - Nephrology, Dialysis, Transplantation VL - vol. 21 DO - http://dx.doi.org/10.1093/ndt/gfl495 ER - TY - JOUR AU - Poux, C.M. AU - Chevret, P. AU - Huchon, D. AU - Jong, W.W.W. de AU - Douzery, E.J. PY - 2006 UR - https://hdl.handle.net/2066/36112 TI - Arrival and diversification of caviomorph rodents and platyrrhine primates in America EP - 244 SN - 1063-5157 IS - iss. 2 SP - 228 JF - Systematic Biology VL - vol. 55 DO - http://dx.doi.org/10.1080/10635150500481390 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Otte-Holler, I. AU - Kamps, B. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2006 UR - https://hdl.handle.net/2066/35406 AB - Small heat shock proteins Hsp20 and HspB2/B3 co-localize with Abeta deposition in senile plaques and cerebral amyloid angiopathy in Alzheimer's disease brains, respectively. It was the aim of our study to investigate if these and other sHsps bind to wild-type Abeta1-42 or the more toxic Abeta1-40 carrying the 'Dutch' mutation (22Glu-->Gln) (D-Abeta1-40), affect Abeta aggregation and thereby influence Abeta cytotoxicity. Binding affinity between sHsps and Abeta was investigated by surface plasmon resonance. Abeta aggregation was studied by using circular dichroism spectroscopy and electron microscopy. Furthermore, we used cultured cerebrovascular cells to investigate the effects of sHsps on Abeta-mediated cytotoxicity. Hsp20, Hsp27 and alphaB-crystallin, but not HspB2/B3, bound to Abeta (both D-Abeta1-40 and Abeta1-42) and reduced or completely inhibited aggregation of D-Abeta1-40 into mature fibrils but did not affect Abeta1-42 aggregation. Furthermore, these sHsps were effective inhibitors of the cerebrovascular toxicity of Abeta (both D-Abeta1-40 and Abeta1-42) in vitro. Binding affinity of the sHsps to D-Abeta1-40 correlated to the degree of inhibition of Abeta-mediated cytotoxicity and the potential to reduce Abeta beta-sheet and fibril formation. With Abeta1-42, a similar correlation between binding affinity and cytotoxicity was observed, but not with its aggregation state. In conclusion, sHsps may regulate Abeta aggregation and serve as antagonists of the biological action of Abeta, but the extent of their interaction depends on the type of sHsp and Abeta peptide. TI - Small heat shock proteins inhibit amyloid-beta protein aggregation and cerebrovascular amyloid-beta protein toxicity. EP - 78 SN - 0006-8993 IS - iss. 1 SP - 67 JF - Brain Research VL - vol. 1089 DO - http://dx.doi.org/10.1016/j.brainres.2006.03.058 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Otte-Holler, I. AU - Kamps, B. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2006 UR - https://hdl.handle.net/2066/35407 TI - Small heat shock proteins inhibit amyloid-beta protein aggregation and ovascular amyloid-beta protein toxicity EP - 78 SN - 0006-8993 IS - iss. 1 SP - 67 JF - Brain Research VL - vol. 1089 DO - http://dx.doi.org/10.1016/j.brainres.2006.03.058 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Otte-Holler, I. AU - Kamps, B. AU - Kusters, B. AU - Maat-Schieman, M.L. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2006 UR - https://hdl.handle.net/2066/35404 AB - Alzheimer's disease (AD) is characterized by pathological lesions, such as senile plaques (SPs) and cerebral amyloid angiopathy (CAA), both predominantly consisting of a proteolytic cleavage product of the amyloid-beta precursor protein (APP), the amyloid-beta peptide (Abeta). CAA is also the major pathological lesion in hereditary cerebral hemorrhage with amyloidosis of the Dutch type (HCHWA-D), caused by a mutation in the gene coding for the Abeta peptide. Several members of the small heat shock protein (sHsp) family, such as alphaB-crystallin, Hsp27, Hsp20 and HspB2, are associated with the pathological lesions of AD, and the direct interaction between sHsps and Abeta has been demonstrated in vitro. HspB8, also named Hsp22 of H11, is a recently discovered member of the sHsp family, which has chaperone activity and is observed in neuronal tissue. Furthermore, HspB8 affects protein aggregation, which has been shown by its ability to prevent formation of mutant huntingtin aggregates. The aim of this study was to investigate whether HspB8 is associated with the pathological lesions of AD and HCHWA-D and whether there are effects of HspB8 on Abeta aggregation and Abeta-mediated cytotoxicity. We observed the expression of HspB8 in classic SPs in AD brains. In addition, HspB8 was found in CAA in HCHWA-D brains, but not in AD brains. Direct interaction of HspB8 with Abeta(1-42), Abeta(1-40) and Abeta(1-40) with the Dutch mutation was demonstrated by surface plasmon resonance. Furthermore, co-incubation of HspB8 with D-Abeta(1-40) resulted in the complete inhibition of D-Abeta(1-40)-mediated death of cerebrovascular cells, likely mediated by a reduction in both the beta-sheet formation of D-Abeta(1-40) and its accumulation at the cell surface. In contrast, however, with Abeta(1-42), HspB8 neither affected beta-sheet formation nor Abeta-mediated cell death. We conclude that HspB8 might play an important role in regulating Abeta aggregation and, therefore, the development of classic SPs in AD and CAA in HCHWA-D. TI - Small heat shock protein HspB8: its distribution in Alzheimer's disease brains and its inhibition of amyloid-beta protein aggregation and cerebrovascular amyloid-beta toxicity. EP - 149 SN - 0001-6322 IS - iss. 2 SP - 139 JF - Acta Neuropathologica VL - vol. 111 DO - http://dx.doi.org/10.1007/s00401-005-0030-z ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Boelens, W.C. AU - Otte-Holler, I. AU - Kamps, B. AU - Kusters, B. AU - Maat-Schieman, M.L. AU - Waal, R.M.W. de AU - Verbeek, M.M. PY - 2006 UR - https://hdl.handle.net/2066/35405 TI - Small heat shock protein HspB8: its distribution in Alzheimer's disease brains s inhibition of amyloid-beta protein aggregation and cerebrovascular d-beta toxicity. EP - 149 SN - 0001-6322 IS - iss. 2 SP - 139 JF - Acta Neuropathologica VL - vol. 111 DO - http://dx.doi.org/10.1007/s00401-005-0030-z ER - TY - JOUR AU - Mastronardi, F.G. AU - Wood, D.D. AU - Mei, J. AU - Raijmakers, R. AU - Tseveleki, V. AU - Dosch, H.M. AU - Probert, L.C.B. AU - Moscarello, M.A. PY - 2006 UR - https://hdl.handle.net/2066/35746 TI - Increased citrullination of histone H3 in MS brain and animal models of demyelination:A role for TNF-induced PAD4 translocation EP - 11396 SN - 0270-6474 IS - iss. 44 SP - 11387 JF - The Journal of Neuroscience VL - vol. 26 DO - http://dx.doi.org/10.1523/JNEUROSCI.3349-06.2006 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35746/35746.pdf?sequence=1 ER - TY - JOUR AU - Vannini, A. AU - Cheung, K. AU - Fusconi, M. AU - Stammen-Vogelzangs, J. AU - Drenth, J.P.H. AU - Dall'aglio, A.C. AU - Bianchi, F.B. AU - Bakker-Jonges, L.E. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. AU - Zendman, A.J.W. PY - 2006 UR - https://hdl.handle.net/2066/187165 TI - Anti-CCP2 positivity in non-ra disease samples: citrulline-dependent or not? SN - 0003-4967 JF - Annals of the Rheumatic Diseases VL - vol. Sep 2006; ER - TY - JOUR AU - Bruijn, D.R.H. de AU - Allander, S.V. AU - Dijk, A.H.A. van AU - Willemse, M.P. AU - Thijssen, J. AU - Groningen, J.J.M. van AU - Meltzer, P.S. AU - Geurts van Kessel, A.H.M. PY - 2006 UR - https://hdl.handle.net/2066/35200 AB - Fusion of the SS18 and either one of the SSX genes is a hallmark of human synovial sarcoma. The SS18 and SSX genes encode nuclear proteins that exhibit opposite transcriptional activities. The SS18 protein functions as a transcriptional coactivator and is associated with the SWI/SNF complex, whereas the SSX proteins function as transcriptional corepressors and are associated with the polycomb complex. The domains involved in these opposite transcriptional activities are retained in the SS18-SSX fusion proteins. Here, we set out to determine the direct transcriptional consequences of conditional SS18-SSX2 fusion protein expression using complementary DNA microarray-based profiling. By doing so, we identified several clusters of SS18-SSX2-responsive genes, including a group of genes involved in cholesterol synthesis, which is a general characteristic of malignancy. In addition, we identified a group of SS18-SSX2-responsive genes known to be specifically deregulated in primary synovial sarcomas, including IGF2 and CD44. Furthermore, we observed an uncoupling of EGR1, JUNB, and WNT signaling in response to SS18-SSX2 expression, suggesting that the SWI/SNF-associated coactivation functions of the SS18 moiety are impaired. Finally, we found that SS18-SSX2 expression affects histone modifications in the CD44 and IGF2 promoters and DNA methylation levels in the IGF2 imprinting control region. Together, we conclude that the SS18-SSX2 fusion protein may act as a so-called transcriptional "activator-repressor," which induces downstream target gene deregulation through epigenetic mechanisms. Our results may have implications for both the development and clinical management of synovial sarcomas. (Cancer Res 2006; 66(19): 9474-82). TI - The Synovial Sarcoma-Associated SS18-SSX2 Fusion Protein Induces Epigenetic Gene (De)Regulation. EP - 9482 SN - 0008-5472 IS - iss. 19 SP - 9474 JF - Cancer Research VL - vol. 66 DO - https://doi.org/10.1158/0008-5472.CAN-05-3726 ER - TY - JOUR AU - Bronner, I.M. AU - Meulen, M.F. van der AU - Visser, M. de AU - Kalmijn, S. AU - Venrooij, W.J.W. van AU - Voskuyl, A.E. AU - Dinant, H.J. AU - Linssen, W.H. AU - Wokke, J.H.J. AU - Hogendijk, J.E. PY - 2006 UR - https://hdl.handle.net/2066/35680 TI - Long-term outcome in polymyositis and dermatomyositis EP - 1461 SN - 0003-4967 IS - iss. 11 SP - 1456 JF - Annals of the Rheumatic Diseases VL - vol. 65 DO - https://doi.org/10.1136/ard.2005.045690 ER - TY - JOUR AU - Marin Vinader, L. AU - Genesen, S.T. van AU - Lubsen, N.H. PY - 2006 UR - https://hdl.handle.net/2066/34640 TI - mRNA made during heat shock enters the first round of translation EP - 542 SN - 0006-3002 IS - iss. 11-12 SP - 535 JF - Biochimica et Biophysica Acta VL - vol. 1759 ER - TY - JOUR AU - Rheede, T. van AU - Bastiaans, T. AU - Boone, D.N. AU - Hedges, S.B. AU - Jong, W.W.W. de AU - Madsen, O. PY - 2006 UR - https://hdl.handle.net/2066/35173 TI - The platypus is in its place: nuclear genes and indels confirm the sister group on of monotremes and Therians EP - 597 SN - 0737-4038 IS - iss. 3 SP - 587 JF - Molecular Biology and Evolution VL - vol. 23 DO - https://doi.org/10.1093/molbev/msj064 ER - TY - JOUR AU - Pruijn, G.J.M. PY - 2006 UR - https://hdl.handle.net/2066/35197 TI - The RNA interference pathway: a new target for autoimmunity. EP - 110 SN - 1478-6354 IS - iss. 8 SP - 4 JF - Arthritis Research & Therapy VL - vol. 8 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35197/35197.pdf?sequence=1 ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Otte-Holler, I. AU - Wesseling, P. AU - Waal, R.M.W. de AU - Boelens, W.C. AU - Verbeek, M.M. PY - 2006 UR - https://hdl.handle.net/2066/35352 AB - The small heat shock protein family (sHsp) comprises molecular chaperones able to interact with incorrectly folded proteins. Alzheimer's disease (AD) is characterized by pathological lesions such as senile plaques (SPs), cerebral amyloid angiopathy (CAA) and neurofibrillary tangles (NFTs), predominantly consisting of the incorrectly folded proteins amyloid-beta (Abeta) and tau respectively. The aim of this study was to investigate the association of the chaperones Hsp20, HspB2, alphaB-crystallin and Hsp27 with the pathological lesions of AD brains. For this purpose, a panel of well-characterized antibodies directed against these sHsps was used in immunohistochemistry and immunoblotting. We observed extracellular expression of Hsp20, Hsp27 and HspB2 in classic SPs, and Hsp20 expression in diffuse SPs. In addition, extracellular expression of HspB2 was observed in CAA. Both Hsp27 and alphaB-crystallin were also observed in astrocytes associated with both SPs and CAA. Furthermore, none of the sHsps were observed in NFTs in AD brains. We conclude that specific sHsp species may be involved in the pathogenesis of either SPs or CAA in AD. TI - Specific association of small heat shock proteins with the pathological hallmarks of Alzheimer's disease brains. EP - 130 SN - 0305-1846 IS - iss. 2 SP - 119 JF - Neuropathology and Applied Neurobiology VL - vol. 32 DO - https://doi.org/10.1111/j.1365-2990.2006.00689.x ER - TY - JOUR AU - Wilhelmus, M.M.M. AU - Otte-Holler, I. AU - Wesseling, P. AU - Waal, R.M.W. de AU - Boelens, W.C. AU - Verbeek, M.M. PY - 2006 UR - https://hdl.handle.net/2066/35353 TI - Specific association of small heat shock proteins with the pathological rks of Alzheimer's disease brains EP - 130 SN - 0305-1846 IS - iss. 2 SP - 119 JF - Neuropathology and Applied Neurobiology VL - vol. 32 DO - https://doi.org/10.1111/j.1365-2990.2006.00689.x ER - TY - JOUR AU - Zendman, A.J.W. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2006 UR - https://hdl.handle.net/2066/34914 TI - Use and significance of anti-CCP autoantibodies in rheumatoid arthritis EP - 25 SN - 1462-0324 IS - iss. 1 SP - 20 JF - Rheumatology VL - vol. 45 ER - TY - JOUR AU - Swart, G.W.M. PY - 2006 UR - https://hdl.handle.net/2066/36038 TI - Cell 'guidling' EP - 351 SN - 1469-221X IS - iss. 4 SP - 348 JF - EMBO Reports VL - vol. 7 DO - https://doi.org/10.1038/sj.embor.7400666 ER - TY - JOUR AU - Schilders, G.W. AU - Dijk, E.L. van AU - Raijmakers, R. AU - Pruijn, G.J.M. PY - 2006 UR - https://hdl.handle.net/2066/36039 TI - Cell and molecular biology of the exosome: how to make or break an RNA EP - 208 SN - 0074-7696 SP - 159 JF - International Review of Cytology VL - vol. 251 DO - https://doi.org/10.1016/S0074-7696(06)51005-8 ER - TY - JOUR AU - Venrooij, W.J.W. van AU - Zendman, A.J.W. AU - Pruijn, G.J.M. PY - 2006 UR - https://hdl.handle.net/2066/36060 TI - Autoantibodies to citrullinated antigens in (early) rheumatoid arthritis EP - 41 SN - 1568-9972 IS - iss. 1 SP - 37 JF - Autoimmunity Reviews VL - vol. 6 DO - https://doi.org/10.1016/j.autrev.2006.03.008 ER - TY - JOUR AU - Marin Vinader, L. AU - Onnekink, C. AU - Genesen, S.T. van AU - Slingsby, C. AU - Lubsen, N.H. PY - 2006 UR - https://hdl.handle.net/2066/35742 TI - In vivo heteromer formation. Expression of soluble betaA4-crystallin requires ession of a heteromeric partner EP - 3182 SN - 1742-464X IS - iss. 14 SP - 3172 JF - Febs Journal VL - vol. 273 ER - TY - JOUR AU - Doerwald, L. AU - Genesen, S.T. van AU - Onnekink, C. AU - Marin Vinader, L. AU - Lange, F. de AU - Jong, W.W.W. de AU - Lubsen, N.H. PY - 2006 UR - https://hdl.handle.net/2066/35212 TI - The effect of alphaB-crystallin and Hsp27 on the availability of translation tion factors in heat-shocked cells EP - 743 SN - 1420-682X IS - iss. 3 SP - 735 JF - Cellular and Molecular Life Sciences VL - vol. 63 DO - https://doi.org/10.1007/s00018-005-5582-5 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Vree Egberts, W.T.M. AU - Seelig, H.P. AU - Lundberg, I.E. AU - Moutsopoulos, H.M. AU - Doria, A. AU - Mosca, M. AU - Vencovsky, J. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2006 UR - https://hdl.handle.net/2066/35996 AB - OBJECTIVES: To assess the clinical implications of autoantibodies directed against different parts of the Mi-2 beta autoantigen in patients with myositis. METHODS: A systematic assessment of the clinical, laboratory, and histological characteristics of 48 anti-Mi-2 positive patients from six European centres was made. Anti-Mi-2 autoantibodies were determined with an ELISA using four overlapping fragments spanning the entire amino acid sequence of the autoantigen. Data were compared with results for a large group of anti-Mi-2 negative patients with myositis published previously. RESULTS: Anti-Mi-2 autoantibodies were found in dermatomyositis, polymyositis, and inclusion body myositis. In general, myositis with anti-Mi-2 autoantibodies was characterised by relatively mild disease, sometimes accompanied by extra-muscular symptoms, including arthralgia, arthritis, Raynaud's phenomenon, and interstitial lung disease. Cardiac disease was not seen, and treatment response was fair. No differences were found between patients with autoantibodies to different fragments of the Mi-2 beta antigen, except for a potentially increased risk of cancer in patients with antibodies directed to the N-terminal fragment of the autoantigen. CONCLUSIONS: Anti-Mi-2 autoantibodies are not a marker of a specific subtype of myositis. No significant differences between patients with autoantibodies to different fragments of the Mi-2 beta autoantigen are found, with the possible exception of an increased risk of cancer in patients with antibodies to the N-terminal fragment. TI - Clinical characteristics of patients with myositis and autoantibodies to different fragments of the Mi-2 beta antigen. EP - 245 SN - 0003-4967 IS - iss. 2 SP - 242 JF - Annals of the Rheumatic Diseases VL - vol. 65 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Laak, H.J. ter AU - Vree Egberts, W.T.M. AU - Lundberg, I.E. AU - Moutsopoulos, H.M. AU - Vencovsky, J. AU - Doria, A. AU - Mosca, M. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2006 UR - https://hdl.handle.net/2066/36099 TI - Anti-signal recognition particle autoantibodies: marker of a necrotising hy EP - 1638 SN - 0003-4967 IS - iss. 12 SP - 1635 JF - Annals of the Rheumatic Diseases VL - vol. 65 DO - https://doi.org/10.1136/ard.2006.052191 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Laak, H.J. ter AU - Vree Egberts, W.T.M. AU - Lundberg, I.E. AU - Moutsopoulos, H.M. AU - Vencovsky, J. AU - Doria, A. AU - Mosca, M. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2006 UR - https://hdl.handle.net/2066/36100 AB - OBJECTIVE: To elucidate the clinical importance of the anti-signal recognition particle (SRP) autoantibody in patients with myositis. METHODS: Retrospective systematic assessment of the clinical, laboratory and histological characteristics of 23 anti-SRP-positive patients from six European centres. Data were compared with a large group of anti-SRP-negative patients with myositis published previously. RESULTS: Clinically, patients with anti-SRP autoantibodies often had a severe symmetric proximal muscle weakness resulting in marked disability, dysphagia and highly elevated levels of serum creatine kinase. Three patients had typical dermatomyositis rashes. The disease was associated with the occurrence of extramuscular signs and symptoms including interstitial lung disease. No association was found with an increased risk of cardiac involvement, and the disease carried a reasonably favourable prognosis with most patients responding to treatment. None of the patients had the typical histological features of myositis. Most muscle biopsy specimens showed the presence of necrotic muscle fibres and no inflammatory infiltrates. CONCLUSIONS: Anti-SRP autoantibodies are associated with a syndrome of a necrotising myopathy in the spectrum of immune-mediated myopathies that differs from typical polymyositis. Further studies are needed to elucidate the pathogenesis and to clarify the role of the anti-SRP autoantibodies in this unique disease. TI - Anti-signal recognition particle autoantibodies: marker of a necrotising myopathy. EP - 1638 SN - 0003-4967 IS - iss. 12 SP - 1635 JF - Annals of the Rheumatic Diseases VL - vol. 65 DO - https://doi.org/10.1136/ard.2006.052191 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Vree Egberts, W.T.M. AU - Seelig, H.P. AU - Lundberg, I.E. AU - Moutsopoulos, H.M. AU - Doria, A. AU - Mosca, M. AU - Vencovsky, J. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2006 UR - https://hdl.handle.net/2066/187176 TI - Clinical characteristics of patients with myositis and autoantibodies to ent fragments of the Mi-2 beta antigen EP - 245 SN - 0003-4967 IS - iss. 2 SP - 242 JF - Annals of the Rheumatic Diseases VL - vol. 65 ER - TY - JOUR AU - Roelofs, M.F. AU - Boelens, W.C. AU - Joosten, L.A.B. AU - Abdollahi-Roodsaz, S. AU - Geurts, J. AU - Wunderink, L.U. AU - Schreurs, B.W. AU - Berg, W.B. van den AU - Radstake, T.R.D.J. PY - 2006 UR - https://hdl.handle.net/2066/35791 AB - Dendritic cells (DCs) are specialized APCs that can be activated upon pathogen recognition as well as recognition of endogenous ligands, which are released during inflammation and cell stress. The recognition of exogenous and endogenous ligands depends on TLRs, which are abundantly expressed in synovial tissue from rheumatoid arthritis (RA) patients. Furthermore TLR ligands are found to be present in RA serum and synovial fluid and are significantly increased, compared with serum and synovial fluid from healthy volunteers and patients with systemic sclerosis and systemic lupus erythematosus. Identification of novel endogenous TLR ligands might contribute to the elucidation of the role of TLRs in RA and other autoimmune diseases. In this study, we investigated whether five members of the small heat shock protein (HSP) family were involved in TLR4-mediated DC activation and whether these small HSPs were present in RA synovial tissue. In vitro, monocyte-derived DCs were stimulated with recombinant alphaA crystallin, alphaB crystallin, HSP20, HSPB8, and HSP27. Using flow cytometry and multiplex cytokine assays, we showed that both alphaA crystallin and HSPB8 were able to activate DCs and that this activation was TLR4 dependent. Furthermore, Western blot and immunohistochemistry showed that HSPB8 was abundantly expressed in synovial tissue from patients with RA. With these experiments, we identified sHSP alphaA crystallin and HSPB8 as two new endogenous TLR4 ligands from which HSPB8 is abundantly expressed in RA synovial tissue. These findings suggest a role for HSPB8 during the inflammatory process in autoimmune diseases such as RA. TI - Identification of small heat shock protein B8 (HSP22) as a novel TLR4 ligand and potential involvement in the pathogenesis of rheumatoid arthritis. EP - 7027 SN - 0022-1767 IS - iss. 11 SP - 7021 JF - Journal of Immunology VL - vol. 176 DO - https://doi.org/10.4049/jimmunol.176.11.7021 ER - TY - JOUR AU - Malecaze, F. AU - Lubsen, N.H. AU - Serre, B. AU - Decha, A. AU - Duboue, M. AU - Penary, M. AU - Berg, D. AU - Arnaud, J.D. AU - Titeux, M. AU - Kremer, E.J. AU - Couderc, B. PY - 2006 UR - https://hdl.handle.net/2066/35533 TI - Prevention of posterior capsule opacification by the induction of therapeutic sis of residual lens cells EP - 448 SN - 0969-7128 IS - iss. 5 SP - 440 JF - Gene Therapy VL - vol. 13 DO - https://doi.org/10.1038/sj.gt.3302667 ER - TY - JOUR AU - Narayanan, S. AU - Kamps, B. AU - Boelens, W.C. AU - Reif, B. PY - 2006 UR - https://hdl.handle.net/2066/36141 TI - AlphaB-crystallin competes with Alzheimer's disease beta-amyloid peptide for e-peptide interactions and induces oxidation of Abeta-Met35 EP - 5946 SN - 1873-3468 IS - iss. 25 SP - 5941 JF - FEBS Letters VL - vol. 580 DO - https://doi.org/10.1016/j.febslet.2006.09.063 ER - TY - JOUR AU - Hof, D. AU - Cheung, K. AU - Roossien, H.E. AU - Pruijn, G.J.M. AU - Raats, J.M.H. PY - 2006 UR - https://hdl.handle.net/2066/36171 TI - A novel subtractive antibody phage display method to discover disease markers EP - 255 SN - 1535-9476 IS - iss. 2 SP - 245 JF - Molecular & Cellular Proteomics VL - vol. 5 DO - https://doi.org/10.1074/mcp.M500239-MC200 ER - TY - JOUR AU - Boros, S. AU - Ahrman, E. AU - Wunderink, L AU - Kamps, B. AU - Jong, W.W.W. de AU - Boelens, W.C. AU - Emanuelsson, C.S. PY - 2006 UR - https://hdl.handle.net/2066/35399 TI - Site-specific transamidation and deamidation of the small heat-shock protein by tissue transglutaminase. EP - 1052 SN - 0887-3585 IS - iss. 4 SP - 1044 JF - Proteins-Structure Function and Bioinformatics VL - vol. 62 DO - https://doi.org/10.1002/prot.20837 ER - TY - JOUR AU - Welting, T.J.M. AU - Kikkert, B.J. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2006 UR - https://hdl.handle.net/2066/35927 TI - Differential association of protein subunits with the human RNase MRP and RNase lexes EP - 1382 SN - 1355-8382 IS - iss. 7 SP - 1373 JF - Rna : a Publication of the Rna Society VL - vol. 12 DO - https://doi.org/10.1261/rna.2293906 ER - TY - JOUR AU - Wessels, E. AU - Duijsings, D.M. AU - Lanke, K.H.W. AU - Dooren, S.H.J. van AU - Jackson, C.L. AU - Melchers, W.J.G. AU - Kuppeveld, F.J.M. van PY - 2006 UR - https://hdl.handle.net/2066/35907 AB - The 3A protein of the coxsackievirus B3 (CVB3), an enterovirus that belongs to the family of the picornaviruses, inhibits endoplasmic reticulum-to-Golgi transport. Recently, we elucidated the underlying mechanism by showing that CVB3 3A interferes with ADP-ribosylation factor 1 (Arf1)-dependent COP-I recruitment to membranes by binding and inhibiting the function of GBF1, a guanine nucleotide exchange factor that is required for the activation of Arf1 (E. Wessels et al., Dev. Cell 11:191-201, 2006). Here, we show that the 3A protein of poliovirus, another enterovirus, is also able to interfere with COP-I recruitment through the same mechanism. No interference with protein transport or COP-I recruitment was observed for the 3A proteins of any of the other picornaviruses tested here (human rhinovirus [HRV], encephalomyocarditis virus, foot-and-mouth disease virus, and hepatitis A virus). We show that the 3A proteins of HRV, which are the most closely related to the enteroviruses, are unable to inhibit COP-I recruitment, due to a reduced ability to bind GBF1. When the N-terminal residues of the HRV 3A proteins are replaced by those of CVB3 3A, chimeric proteins are produced that have gained the ability to bind GBF1 and, by consequence, to inhibit protein transport. These results show that the N terminus of the CVB3 3A protein is important for binding of GBF1 and its transport-inhibiting function. Taken together, our data demonstrate that the activity of the enterovirus 3A protein to inhibit GBF1-dependent COP-I recruitment is unique among the picornaviruses. TI - Effects of picornavirus 3A Proteins on Protein Transport and GBF1-dependent COP-I recruitment. EP - 11860 SN - 0022-538X IS - iss. 23 SP - 11852 JF - Journal of Virology VL - vol. 80 DO - https://doi.org/10.1128/JVI.01225-06 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/35907/35907.pdf?sequence=1 ER - TY - JOUR AU - Lunter, P.C. AU - Kilsdonk, J.W.J. van AU - Beek, H. van AU - Cornelissen, I.M. AU - Bergers, A.M.G. AU - Willems, P.H.G.M. AU - Muijen, G.N.P. van AU - Swart, G.W.M. PY - 2005 UR - https://hdl.handle.net/2066/33324 AB - Activated leukocyte cell adhesion molecule (ALCAM/CD166/MEMD) could function as a cell surface sensor for cell density, controlling the transition between local cell proliferation and tissue invasion in melanoma progression. We have tested the hypothesis that progressive cell clustering controls the proteolytic cascade for activation of gelatinase A/matrix metalloproteinase-2 (MMP-2), which involves formation of an intermediate ternary complex of membrane type 1 MMP (MT1-MMP/MMP-14), tissue inhibitor of metalloproteinase-2 (TIMP-2), and pro-MMP-2 at the cell surface. Surprisingly, truncation of ALCAM severely impaired MMP-2 activation in a nude mouse xenograft model, in which we previously observed diminished primary tumor growth and enhanced melanoma metastasis. Comparative studies of two-dimensional monolayer and three-dimensional collagen-gel cultures revealed that extensive cell-to-cell contacts, wild-type ALCAM, and cell-to-matrix interactions were all indispensable for efficient conversion of pro-MMP-2 to its active form in metastatic melanoma cells. Truncated, dominant-negative ALCAM diminished MMP-2 activation via reduced transcript levels and decreased processing of MT1-MMP. Failure of the proteolytic cascade after selective ALCAM depletion by RNA interference was mainly due to incomplete MT1-MMP processing, which was otherwise promoted by extensive cell-to-cell contacts. These data attribute a novel signaling role to ALCAM in regulation of proteolysis and support its previously postulated sensor function in invasive growth. TI - Activated leukocyte cell adhesion molecule (ALCAM/CD166/MEMD), a novel actor in invasive growth, controls matrix metalloproteinase activity. EP - 8808 SN - 0008-5472 IS - iss. 19 SP - 8801 JF - Cancer Research VL - vol. 65 DO - http://dx.doi.org/10.1158/0008-5472.CAN-05-0378 ER - TY - JOUR AU - Raijmakers, R. AU - Vogelzangs, J.H.P. AU - Croxford, J.L. AU - Wesseling, P. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2005 UR - https://hdl.handle.net/2066/32325 AB - Immunization of mammals with central nervous system (CNS)-derived proteins or peptides induces experimental autoimmune encephalomyelitis (EAE), a disease resembling the human autoimmune disease multiple sclerosis (MS). Both diseases are accompanied by destruction of a part of the of the myelin sheaths, which surround neurites in the CNS. Previous studies in MS have described alterations in the citrullination of myelin basic protein, one of the main protein constituents of the myelin sheath. Here, we show that, also during the development of EAE in mice, hypercitrullination occurs in the areas of the spinal cord that show the highest degree of inflammation and that myelin basic protein and glial fibrillary acidic protein are among the hypercitrullinated proteins. We conclude that hypercitrullination of myelin proteins in the CNS is a common phenomenon in demyelinating disease. Hypercitrullination may cause conformational changes in proteins, so the affected proteins may be involved in the pathogenesis of CNS autoimmune disease by acting as autoreactive T-cell epitopes. This is the first report in which hypercitrullination of CNS proteins in EAE is described and in which proteins other than myelin basic protein are reported to be citrullinated during autoimmune-mediated CNS inflammation. TI - Citrullination of central nervous system proteins during the development of experimental autoimmune encephalomyelitis. EP - 253 SN - 0021-9967 IS - iss. 3 SP - 243 JF - Journal of Comparative Neurology VL - vol. 486 DO - http://dx.doi.org/10.1002/cne.20529 ER - TY - JOUR AU - Pruijn, G.J.M. AU - Vossenaar, E.R. AU - Drijfhout, J.W. AU - Venrooij, W.J.W. van AU - Zendman, A.J.W. PY - 2005 UR - https://hdl.handle.net/2066/33294 TI - Anti-CCP antibody detection facilitates early diagnosis and prognosis of rheumatoid arthritis EP - 7 SN - 1573-3971 IS - iss. 1 SP - 1 JF - Current Rheumatology Reviews VL - vol. 1 ER - TY - JOUR AU - Lundberg, K. AU - Nijenhuis, S. AU - Vossenaar, E.R. AU - Palmblad, K. AU - Venrooij, W.J.W. van AU - Klareskog, L. AU - Zendman, A.J.W. AU - Harris, H.E. PY - 2005 UR - https://hdl.handle.net/2066/32323 TI - Citrullinated proteins have increased immunogenicity and arthritogenicity and their presence in arthritic joints correlates with disease severity EP - R467 SN - 1478-6354 IS - iss. 3 SP - R458 JF - Arthritis Research & Therapy VL - vol. 7 DO - http://dx.doi.org/10.1186/ar1697 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/32323/32323.pdf?sequence=1 ER - TY - JOUR AU - Raijmakers, R. AU - Vogelzangs, J.H.P. AU - Croxford, J.L. AU - Wesseling, P. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2005 UR - https://hdl.handle.net/2066/32324 TI - Citrullination of central nervous system proteins during the development of experimental autoimmune encephalomyelitis EP - 253 SN - 0021-9967 IS - iss. 3 SP - 243 JF - Journal of Comparative Neurology VL - vol. 486 DO - http://dx.doi.org/10.1002/cne.20529 ER - TY - JOUR AU - Lunter, P.C. AU - Kilsdonk, J.W.J. van AU - Beek, H. van AU - Cornelissen, L.M.H.A. AU - Bergers, A.M.G. AU - Willems, P.H.G.M. AU - Muijen, G.N.P. van AU - Swart, G.W.M. PY - 2005 UR - https://hdl.handle.net/2066/33323 TI - Activated leukocyte cell adhesion molecule (ALCAM/CD166/MEMD), a novel actor in invasive growth, controls matrix metalloproteinase activity EP - 8808 SN - 0008-5472 IS - iss. 19 SP - 8801 JF - Cancer Research VL - vol. 65 DO - http://dx.doi.org/10.1158/0008-5472.CAN-05-0378 ER - TY - JOUR AU - Ottosson, L. AU - Salomonsson, S. AU - Hennig, J. AU - Sonesson, S.E. AU - Dorner, T. AU - Raats, J.M.H. AU - Kuchroo, V.K. AU - Sunnerhagen, M. AU - Wahren-Herlenius, M. PY - 2005 UR - https://hdl.handle.net/2066/33019 TI - Structurally derived mutations define congenital heart block-related epitipes within the 200-239 amino acid stretch of the R052 protein EP - 118 SN - 0300-9475 IS - iss. 2 SP - 109 JF - Scandinavian Journal of Immunology VL - vol. 61 DO - http://dx.doi.org/10.1111/j.0300-9475.2005.01542.x ER - TY - JOUR AU - Mahler, M. AU - Raijmakers, R. AU - Dähnrich, C. AU - Blüthner, M. AU - Fritzler, M.J. PY - 2005 UR - https://hdl.handle.net/2066/32326 TI - Clinical evaluation of autoantibodies to a novel PM/Scl peptide antigen EP - R713 SN - 1478-6354 IS - iss. 3 SP - R704 JF - Arthritis Research & Therapy VL - vol. 7 DO - http://dx.doi.org/10.1186/ar1729 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/32326/32326.pdf?sequence=1 ER - TY - JOUR AU - Marin Vinader, L. AU - Shin, C. AU - Onnekink, C. AU - Manley, J.L. AU - Lubsen, N.H. PY - 2005 UR - https://hdl.handle.net/2066/32592 TI - Hsp27 enhances recovery of splicing as well as rephosphorylation of SRp38 after heat shock EP - 894 SN - 1059-1524 IS - iss. 2 SP - 886 JF - Molecular Biology of the Cell VL - vol. 17 DO - http://dx.doi.org/10.1091/mbc.E05-07-0596 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/32592/32592.pdf?sequence=1 ER - TY - JOUR AU - Huebner, W. AU - Kidd, B.A. AU - Tomooka, B.H. AU - Lee, B.J. AU - Bruce, B. AU - Fries, J.F. AU - Sonderstrup, G. AU - Monach, P. AU - Drijfhout, J.W. AU - Venrooij, W.J.W. van AU - Utz, P.J. AU - Genovese, M.C. AU - W.H., R. PY - 2005 UR - https://hdl.handle.net/2066/33295 TI - Antigen microarrays profiling of autoantibodies in rheumatoid arthritis EP - 2655 SN - 0004-3591 IS - iss. 9 SP - 2645 JF - Arthritis and Rheumatism VL - vol. 52 DO - https://doi.org/10.1002/art.21269 ER - TY - JOUR AU - Gorgels, T.G. AU - Hu, X. AU - Scheffer, G.L. AU - Wal, A. van der AU - Toonstra, J. AU - Jong, P.T.V.M. de AU - Kuppevelt, A.H.M.S.M. van AU - Levelt, C. AU - Wolf, A. AU - Loves, W.J. AU - Scheper, R.J. AU - Peek, R. AU - Bergen, A.A.B. PY - 2005 UR - https://hdl.handle.net/2066/32422 AB - Pseudoxanthoma elasticum (PXE) is a heritable disorder of connective tissue, affecting mainly skin, eye and the cardiovascular system. PXE is characterized by dystrophic mineralization of elastic fibres. The condition is caused by loss of function mutations in ABCC6. We generated Abcc6 deficient mice (Abcc6-/-) by conventional gene targeting. As shown by light and electron microscopy Abcc6-/- mice spontaneously developed calcification of elastic fibres in blood vessel walls and in Bruch's membrane in the eye. No clear abnormalities were seen in the dermal extracellular matrix. Calcification of blood vessels was most prominent in small arteries in the cortex of the kidney, but in old mice, it occurred also in other organs and in the aorta and vena cava. Newly developed monoclonal antibodies against mouse Abcc6 localized the protein to the basolateral membranes of hepatocytes and the basal membrane in renal proximal tubules, but failed to show the protein at the pathogenic sites. Abcc6-/- mice developed a 25% reduction in plasma HDL cholesterol and an increase in plasma creatinine levels, which may be due to impaired kidney function. No changes in serum mineral balance were found. We conclude that the phenotype of the Abcc6-/- mouse shares calcification of elastic fibres with human PXE pathology, which makes this model a useful tool to further investigate the aetiology of PXE. Our data support the hypothesis that PXE is in fact a systemic disease. TI - Disruption of Abcc6 in the mouse: novel insight in the pathogenesis of pseudoxanthoma elasticum. EP - 1773 SN - 0964-6906 IS - iss. 13 SP - 1763 JF - Human Molecular Genetics VL - vol. 14 DO - https://doi.org/10.1093/hmg/ddi183 ER - TY - JOUR AU - Pruijn, G.J.M. PY - 2005 UR - https://hdl.handle.net/2066/32429 TI - Doughnuts dealing with RNA EP - 564 SN - 1545-9993 IS - iss. 7 SP - 562 JF - Nature Structural & Molecular Biology VL - vol. 12 ER - TY - JOUR AU - Schilders, G.W. AU - Raijmakers, R. AU - Raats, J.M.H. AU - Pruijn, G.J.M. PY - 2005 UR - https://hdl.handle.net/2066/32691 TI - MPP6 is an exosome-associated RNA-binding protein involved in 5.8S rRNA maturation EP - 6804 SN - 0305-1048 IS - iss. 21 SP - 6795 JF - Nucleic Acids Research VL - vol. 33 DO - https://doi.org/10.1093/nar/gki982 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/32691/32691.pdf?sequence=1 ER - TY - JOUR AU - Annunziata, O. AU - Pande, A. AU - Pande, J. AU - Ogun, O. AU - Lubsen, N.H. AU - Benedek, G.B. PY - 2005 UR - https://hdl.handle.net/2066/32805 TI - Oligomerization and phase transitions in aqueous solutions of native and truncated human beta B1-crystallin EP - 1328 SN - 0006-2960 IS - iss. 4 SP - 1316 JF - Biochemistry VL - vol. 44 DO - https://doi.org/10.1021/bi048419f ER - TY - JOUR AU - Rheede, T. van AU - Bastiaans, T. AU - Boone, D.N. AU - Hedges, S.B. AU - Jong, W.W.W. de AU - Madsen, O. PY - 2005 UR - https://hdl.handle.net/2066/33197 TI - The platypus is in its place: nuclear genes and indels confirm the siter group relation of monotremes and therians EP - 597 SN - 0737-4038 IS - iss. 3 SP - 587 JF - Molecular Biology and Evolution VL - vol. 23 DO - https://doi.org/10.1093/molbev/msj064 ER - TY - JOUR AU - Roodink, I. AU - Raats, J.M.H. AU - Van der Zwaag, B. AU - Verrijp, K. AU - Kusters, B. AU - Bokhoven, J.H.L.M. van AU - Linkels, M. AU - Waal, R.M.W. de AU - Leenders, W.P.J. PY - 2005 UR - https://hdl.handle.net/2066/32853 TI - Plexin D1 expression is induced on tumor vasculature and tumor cells: a novel target for diagnosis and therapy? EP - 8323 SN - 0008-5472 IS - iss. 18 SP - 8317 JF - Cancer Research VL - vol. 65 DO - https://doi.org/10.1158/0008-5472.CAN-04-4366 ER - TY - JOUR AU - Shimeld, S.M. AU - Purkiss, A.G. AU - Dirks, R.P.H. AU - Bateman, O.A. AU - Slingsby, C AU - Lubsen, N.H. PY - 2005 UR - https://hdl.handle.net/2066/33139 TI - Urochordate betagamma-crystallin and the evolutionary origin of the vertebrate eye lens EP - 1689 SN - 0960-9822 IS - iss. 18 SP - 1684 JF - Current Biology VL - vol. 15 DO - https://doi.org/10.1016/j.cub.2005.08.046 ER - TY - JOUR AU - Roodink, I. AU - Raats, J.M.H. AU - Zwaag, B. van der AU - Verrijp, K. AU - Kusters, B. AU - Bokhoven, J.H.L.M. van AU - Linkels, M. AU - Waal, R.M.W. de AU - Leenders, W.P.J. PY - 2005 UR - https://hdl.handle.net/2066/32854 AB - We previously reported that during mouse embryogenesis, plexin D1 (plxnD1) is expressed on neuronal and endothelial cells. Endothelial cells gradually loose plxnD1 expression during development. Here we describe, using in situ hybridization, that endothelial plxnD1 expression is regained during tumor angiogenesis in a mouse model of brain metastasis. Importantly, we found PLXND1 expression also in a number of human brain tumors, both of primary and metastatic origin. Apart from the tumor vasculature, abundant expression was also found on tumor cells. Via panning of a phage display library, we isolated two phages that carry single-domain antibodies with specific affinity towards a PLXND1-specific peptide. Immunohistochemistry with these single-domain antibodies on the same tumors that were used for in situ hybridization confirmed PLXND1 expression on the protein level. Furthermore, both these phages and the derived antibodies specifically homed to vessels in brain lesions of angiogenic melanoma in mice after i.v. injection. These results show that PLXND1 is a clinically relevant marker of tumor vasculature that can be targeted via i.v. injections. TI - Plexin D1 expression is induced on tumor vasculature and tumor cells: a novel target for diagnosis and therapy? EP - 8323 SN - 0008-5472 IS - iss. 18 SP - 8317 JF - Cancer Research VL - vol. 65 DO - https://doi.org/10.1158/0008-5472.CAN-04-4366 ER - TY - JOUR AU - Poux, C.M. AU - Madsen, O. AU - Marquard, E. AU - Vieites, D. AU - Jong, W. de AU - Vences, M. PY - 2005 UR - https://hdl.handle.net/2066/33251 TI - Asynchronous colonization of Madagascar by the four endemic clades of primates, tenrecs, carnivores, and rodents as inferred from nuclear genes EP - 730 SN - 1063-5157 IS - iss. 5 SP - 719 JF - Systematic Biology VL - vol. 54 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/33251/33251.pdf?sequence=1 ER - TY - JOUR AU - Swart, G.W.M. AU - Lunter, P.C. AU - Kilsdonk, J.W.J. van AU - Kempen, L.C.L.T. van PY - 2005 UR - https://hdl.handle.net/2066/33322 TI - Activated leukocyte cell adhesion molecule (ALCAM/CD166): signaling at the divide of melanoma cell clustering and cell migration? EP - 236 SN - 0167-7659 IS - iss. 2 SP - 223 JF - Cancer and Metastasis Reviews VL - vol. 24 DO - https://doi.org/10.1007/s10555-005-1573-0 ER - TY - JOUR AU - Swart, G.W.M. AU - Lunter, P.C. AU - Kilsdonk, J.W.J. van AU - Kempen, L.C.L.T. van PY - 2005 UR - https://hdl.handle.net/2066/33321 AB - Orchestrated modulation of cell adhesion is essential for development and homeostasis in multicellular organisms. It optimizes embedding of the cell in its dynamic environment and facilitates appropriate cell responses and intercellular communication. Chronic disturbance of this delicate equilibrium causes defects in tissue architecture and sometimes cancer. In tumor cell biology, dynamic control of adhesion molecules is important to proceed through the metastatic cascade and to allow cell release from the primary tumor, invasion of the surrounding matrix, intravasation and adhesion to vascular endothelial cells to facilitate extravasation. Intertwined and multiple adhesive interactions rather than individual interactions presumably play critical roles in neoplastic development. Yet, knowledge of the contribution of each individual adhesion molecule is essential to unravel this network of interactions. This review will focus on activated leukocyte cell adhesion molecule (ALCAM/CD166) and its role in human melanoma progression. It is hypothesized that ALCAM may function as a cell surface sensor to register local growth saturation and to regulate cellular signaling and dynamic responses. TI - Activated leukocyte cell adhesion molecule (ALCAM/CD166): signaling at the divide of melanoma cell clustering and cell migration? EP - 236 SN - 0167-7659 IS - iss. 2 SP - 223 JF - Cancer and Metastasis Reviews VL - vol. 24 DO - https://doi.org/10.1007/s10555-005-1573-0 ER - TY - JOUR AU - Stamler, R. AU - Kappe, G. AU - Boelens, W.C. AU - Slingsby, C PY - 2005 UR - https://hdl.handle.net/2066/33086 TI - Wrapping the alpha-crystallin domain fold in a chaperone assembly EP - 79 SN - 0022-2836 IS - iss. 1 SP - 68 JF - Journal of Molecular Biology VL - vol. 353 DO - https://doi.org/10.1016/j.jmb.2005.08.025 ER - TY - JOUR AU - Sundby, C. AU - Boros, S. AU - Hjernoe, K. AU - Boelens, W.C. AU - Hojrup, P. PY - 2005 UR - https://hdl.handle.net/2066/32963 TI - Screening for transglutaminase-catalyzed modifications by peptide mass finger-printing using multi-point recalibration on recognized peaks for high mass accuracy EP - 208 SN - 1524-0215 IS - iss. 3 SP - 197 JF - Journal of Biomolecular Techniques VL - vol. 16 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Brenk, L. van AU - Vree Egberts, W.T.M. AU - Kooi, E.L. van der AU - Borm, G.F. AU - Padberg, G.W.A.M. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2005 UR - https://hdl.handle.net/2066/32585 AB - Myositis specific autoantibodies (MSAs) are proven to be specific for myositis compared with other inflammatory connective tissue diseases. Their specificity compared, however, with other neuromuscular disorders, which are included in the differential diagnosis of patients in whom the diagnosis myositis is under consideration, is unknown. We prospectively screened sera from 107 patients with various neuromuscular disorders for the most common MSAs and compared the results with the findings in a group of 97 myositis patients, published previously. Special attention was paid to patients with facioscapulohumeral muscular dystrophy (FSHD), an autosomal dominant muscle disease with marked inflammation in skeletal muscle tissue.Only one patient in the neuromuscular disorders group tested positive for an MSA, compared with 41 in the myositis group, resulting in a specificity of 99%. None of the FSHD patients tested positive. We conclude that the tested MSAs are highly specific for myositis and that they are not merely associated with muscle inflammation. TI - High specificity of myositis specific autoantibodies for myositis compared with other neuromuscular disorders. EP - 537 SN - 0340-5354 IS - iss. 5 SP - 534 JF - Journal of Neurology VL - vol. 252 DO - https://doi.org/10.1007/s00415-005-0683-5 ER - TY - JOUR AU - Boros, S. AU - Ahrman, E. AU - Wunderink, L AU - Kamps, B. AU - Jong, W.W.W. de AU - Boelens, W.C. AU - Emanuelsson, C.S. PY - 2005 UR - https://hdl.handle.net/2066/32990 TI - Site-specific transamidation and deamidation of the small heat-shock protein Hsp20 by tissue transglutaminase EP - 1052 SN - 0887-3585 IS - iss. 4 SP - 1044 JF - Proteins-Structure Function and Bioinformatics VL - vol. 62 DO - https://doi.org/10.1002/prot.20837 ER - TY - JOUR AU - Hof, D. AU - Cheung, K. AU - Roossien, H.E. AU - Pruijn, G.J.M. AU - Raats, J.M.H. PY - 2005 UR - https://hdl.handle.net/2066/33360 TI - A novel subtractive antobidy phage display method to discover disease markers EP - 255 SN - 1535-9476 IS - iss. 2 SP - 245 JF - Molecular & Cellular Proteomics VL - vol. 5 DO - https://doi.org/10.1074/mcp.M500239-MC200 ER - TY - JOUR AU - Gerritsen, D.L. AU - Jongenelis, K. AU - Pot, A.M. AU - Beekman, A.T.F. AU - Eisses, A.M.H. AU - Derksen, M. AU - Kluiter, H. AU - Ribbe, M.W. PY - 2005 UR - https://hdl.handle.net/2066/39388 TI - Screening for depression among nursing home patients: diagnostic accuracy of the original 30-item and shortened versions of the Geriatric Depression Scale EP - 1074 SN - 0885-6230 IS - iss. 11 SP - 1067 JF - International Journal of Geriatric Psychiatry VL - vol. 20 DO - https://doi.org/10.1002/gps.1398 ER - TY - JOUR AU - Engelsman, J.P. den AU - Gerrits, D. AU - Jong, W.W.W. de AU - Robbins, J. AU - Kato, K. AU - Boelens, W.C. PY - 2005 UR - https://hdl.handle.net/2066/32794 TI - Nuclear import of alphaB-crystallin is phosphorylation-dependent and hampered by hyperphosphorylation of the myopathy-related mutant R120G EP - 37148 SN - 1083-351X IS - iss. 44 SP - 37139 JF - Journal of Biological Chemistry VL - vol. 280 DO - https://doi.org/10.1074/jbc.M504106200 ER - TY - JOUR AU - Farhoud, M.H. AU - Wessels, H.C.T. AU - Steenbakkers, P.J.M. AU - Mattijssen, S. AU - Wevers, R.A. AU - Engelen, B.G.M. van AU - Jetten, M.S.M. AU - Smeitink, J.A.M. AU - Heuvel, L.P.W.J. van den AU - Keltjens, J.T.M. PY - 2005 UR - https://hdl.handle.net/2066/32883 AB - Methanothermobacter thermautotrophicus is a thermophilic archaeon that produces methane as the end product of its primary metabolism. The biochemistry of methane formation has been extensively studied and is catalyzed by individual enzymes and proteins that are organized in protein complexes. Although much is known of the protein complexes involved in methanogenesis, only limited information is available on the associations of proteins involved in other cell processes of M. thermautotrophicus. To visualize and identify interacting and individual proteins of M. thermautotrophicus on a proteome-wide scale, protein preparations were separated using blue native electrophoresis followed by SDS-PAGE. A total of 361 proteins, corresponding to almost 20% of the predicted proteome, was identified using peptide mass fingerprinting after MALDI-TOF MS. All previously characterized complexes involved in energy generation could be visualized. Furthermore the expression and association of the heterodisulfide reductase and methylviologen-reducing hydrogenase complexes depended on culture conditions. Also homomeric supercomplexes of the ATP synthase stalk subcomplex and the N5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase complex were separated. Chemical cross-linking experiments confirmed that the multimerization of both complexes was not experimentally induced. A considerable number of previously uncharacterized protein complexes were reproducibly visualized. These included an exosome-like complex consisting of four exosome core subunits, which associated with a tRNA-intron endonuclease, thereby expanding the constituency of archaeal exosomes. The results presented show the presence of novel complexes and demonstrate the added value of including blue native gel electrophoresis followed by SDS-PAGE in discovering protein complexes that are involved in catabolic, anabolic, and general cell processes. TI - Protein complexes in the archaeon Methanothermobacter thermautotrophicus analyzed by blue native/SDS-PAGE and mass spectrometry. EP - 1663 SN - 1535-9476 IS - iss. 11 SP - 1653 JF - Molecular & Cellular Proteomics VL - vol. 4 DO - https://doi.org/10.1074/mcp.M500171-MCP200 ER - TY - JOUR AU - Farhoud, M.H. AU - Wessels, H.C.T. AU - Steenbakkers, P.J.M. AU - Mattijssen, S. AU - Wevers, R.A. AU - Engelen, B.G.M. van AU - Jetten, M.S.M. AU - Smeitink, J.A.M. AU - Heuvel, L.P.W.J. van den AU - Keltjens, J.T.M. PY - 2005 UR - https://hdl.handle.net/2066/32882 TI - Protein complexes in the archaeon Methanothermobacter thermautotrophicus analyzed by blue native/SDS-PAGE and mass spectrometry EP - 1663 SN - 1535-9476 IS - iss. 11 SP - 1653 JF - Molecular & Cellular Proteomics VL - vol. 4 DO - https://doi.org/10.1074/mcp.M500171-MCP200 ER - TY - JOUR AU - Hof, D. AU - Raats, J.M.H. AU - Pruijn, G.J.M. PY - 2005 UR - https://hdl.handle.net/2066/33298 TI - Apoptotic modifications affect the autoreactivity of the U1 snRNP autoantigen EP - 388 SN - 1568-9972 IS - iss. 6 SP - 380 JF - Autoimmunity Reviews VL - vol. 4 DO - https://doi.org/10.1016/j.autrev.2005.02.003 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Van Brenk, L. AU - Vree Egberts, W.T.M. AU - Kooi, E.L. van der AU - Borm, G.F. AU - Padberg, G.W.A.M. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2005 UR - https://hdl.handle.net/2066/32584 TI - High specificity of myositis specific autoantibodies for myositis compared with other neuromuscular disorders EP - 537 SN - 0340-5354 IS - iss. 5 SP - 534 JF - Journal of Neurology VL - vol. 252 DO - https://doi.org/10.1007/s00415-005-0683-5 ER - TY - JOUR AU - Raats, J.M.H. AU - Hof, D. PY - 2005 UR - https://hdl.handle.net/2066/32909 TI - Recombinant antibody expression vectors enabling double and triple immunostaining of tissue culture cells using monoclonal antibodies EP - 521 SN - 0171-9335 IS - iss. 4 SP - 517 JF - European Journal of Cell Biology VL - vol. 84 DO - https://doi.org/10.1016/j.ejcb.2004.12.025 ER - TY - JOUR AU - Hof, D. AU - Cheung, K. AU - Rooij, D.J.R.A.M. de AU - Hoogen, F.H.J. van den AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van AU - Raats, J.M.H. PY - 2005 UR - https://hdl.handle.net/2066/164896 TI - Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease EP - R309 SN - 1478-6354 IS - iss. 2 SP - R302 JF - Arthritis Research & Therapy VL - vol. 7 DO - https://doi.org/10.1186/ar1490 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/164896/164896.pdf?sequence=1 ER - TY - JOUR AU - Hof, D. AU - Cheung, K. AU - Rooij, D.J.R.A.M. de AU - Hoogen, F.H.J. van den AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van AU - Raats, J.M.H. PY - 2005 UR - https://hdl.handle.net/2066/33255 AB - Modifications occurring on autoantigens during cell death have been proposed to have a role in the initiation of autoimmune diseases. Patients suffering from mixed connective tissue disease (MCTD) produce autoantibodies directed to U1 small nuclear ribonucleoprotein (snRNP), and antibodies against a 70 kDa protein component, the U1-70K (70K) protein, are the most prominent. During apoptosis, 70K is cleaved by caspase-3 to a 40 kDa product, which remains associated with the complex. Autoantibodies preferentially recognizing the apoptotic form of 70K have been described previously, and an apoptosis-specific epitope on 70K has been identified. This study shows that 29 of 53 (54%) MCTD sera preferentially recognize the apoptotic form of 70K over intact 70K. Moreover, we show that antibodies directed to an apoptosis-specific epitope on 70K are more specifically associated with MCTD than other anti-70K antibodies, suggesting that apoptotic 70K is a better antigen for the detection of these antibodies in MCTD patients. Longitudinal analysis of 12 MCTD patients showed in several patients that early sera are relatively enriched with antibodies recognizing an apoptosis-specific epitope, and that the levels of these apoptosis-specific antibodies decrease in time. These findings indicate that the early detection of apoptotic 70K is of considerable interest for anti-U1 snRNP-positive patients. TI - Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease. EP - 9 SN - 1478-6354 IS - iss. 2 SP - R302 JF - Arthritis Research & Therapy VL - vol. 7 DO - https://doi.org/10.1186/ar1490 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/33255/33255.pdf?sequence=1 ER - TY - JOUR AU - Franck, E. AU - Madsen, O. AU - Rheede, T. van AU - Ricard, G.N.S. AU - Huynen, M.A. AU - Jong, W.W.W. de PY - 2004 UR - https://hdl.handle.net/2066/185958 TI - Evolutionary diversity of vertebrate small heat shock proteins EP - 805 SN - 0022-2844 IS - iss. 6 SP - 792 JF - Journal of Molecular Evolution VL - vol. 59 DO - http://dx.doi.org/10.1007/s00239-004-0013-z ER - TY - JOUR AU - Vossenaar, E.R. AU - Zendman, A.J.W. AU - Raijmakers, R. AU - Weiting, T.J. AU - Heijden, A.G. van der AU - Horstman, W.A.M. AU - Cheung, K. AU - Vogelzangs, J.H.P. AU - Nijenhuis, S. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2004 UR - https://hdl.handle.net/2066/58471 TI - Features of the citrullinating peptidylarginine deiminase enzymes EP - S6 SN - 1478-6354 IS - iss. suppl. 1 SP - S5 JF - Arthritis Research & Therapy VL - vol. 6 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/58471/58471.pdf?sequence=1 ER - TY - JOUR AU - Kappe, G. AU - Aquilina, J.A. AU - Wunderink, L AU - Kamps, B. AU - Robinson, C.V. AU - Garate, T AU - Boelens, W.C. AU - Jong, W.W.W. de PY - 2004 UR - https://hdl.handle.net/2066/59182 AB - Small heat shock proteins (sHSPs), which range in monomer size between 12 and 42 kDa, are characterized by a conserved C-terminal alpha-crystallin domain of 80-100 residues. They generally form large homo- or heteromeric complexes, and typically have in vitro chaperone-like activity, keeping unfolding proteins in solution. A special type of sHSP, with a duplicated alpha-crystallin domain, is present in parasitic flatworms (Platyhelminthes). Considering that an alpha-crystallin domain is essential for the oligomerization and chaperone-like properties of sHSPs, we characterized Tsp36 from the tapeworm Taenia saginata. Both wild-type Tsp36 and a mutant (Tsp36C-->R) in which the single cysteine has been replaced by arginine were expressed and purified. Far-UV CD measurements of Tsp36 were in agreement with secondary structure predictions, which indicated alpha-helical structure in the N-terminal region and the expected beta-sandwich structure for the two alpha-crystallin domains. Gel permeation chromatography and nano-ESI-MS showed that wild type Tsp36 forms dimers in a reducing environment, and tetramers in a non-reducing environment. The tetramers are stabilized by disulfide bridges involving a large proportion of the Tsp36 monomers. Tsp36C-->R exclusively occurs as dimers according to gel permeation chromatography, while the nondisulfide bonded fraction of wild type Tsp36 dissociates from tetramers into dimers under nonreducing conditions at increased temperature (43 degrees C). The tetrameric form of Tsp36 has a greater chaperone-like activity than the dimeric form. TI - Tsp36, a tapeworm small heat-shock protein with a duplicated alpha-crystallin domain, forms dimers and tetramers with good chaperone-like activity. EP - 117 SN - 0887-3585 IS - iss. 1 SP - 109 JF - Proteins-Structure Function and Bioinformatics VL - vol. 57 DO - http://dx.doi.org/10.1002/prot.20220 ER - TY - JOUR AU - Mahler, M. AU - Kessenbrock, K. AU - Raats, J.M.H. AU - Fritzler, M.J. PY - 2004 UR - https://hdl.handle.net/2066/57190 AB - Autoantibodies to the three ribosomal phospho (-P) proteins P0, P1, P2, referred to as Rib-P, are specifically found in 10-40% of patients with systemic lupus erythematosus. The variations in the observed frequency of these autoantibodies is related to a number of factors such as the test system used to detect the antibodies. Several immunoassays that were designed for research and diagnostic laboratory use have been developed. The autoantigens employed in these tests include native proteins, recombinant polypeptides, and synthetic peptides. In this study, we compared the technical and clinical accuracy of anti-Rib-P antibody assays from different commercial suppliers including ELISA systems and a novel addressable laser bead assay (from Euroimmun, MBL, Pharmacia Diagnostics, INOVA). Although the assays from all suppliers used in this study performed well in the technical part of the study, relatively poor correlations and significant differences in the clinical accuracy were found. Based on the results, we conclude that the detection of anti-Rib-P antibodies strongly depends on both the nature of the antigen and the detection system. We recommend that anti-Rib-P assays should be standardized on an international level. The Varelisa(R) Rib-P profile and the addressable laser bead Rib-P assays represent promising tools and platforms for the detection of anti-Rib-P antibodies in the future. (C) 2004 Wiley-Liss, Inc. TI - Technical and clinical evaluation of anti-ribosomal P protein immunoassays EP - 223 SN - 0887-8013 IS - iss. 4 SP - 215 JF - Journal of Clinical Laboratory Analysis VL - vol. 18 DO - http://dx.doi.org/10.1002/jcla.20026 ER - TY - JOUR AU - Lundberg, K. AU - Nijenhuis, S. AU - Vossenaar, E.R. AU - Klareskog, L. AU - Venrooij, W.J.W. van AU - Harris, H.E. PY - 2004 UR - https://hdl.handle.net/2066/60098 TI - Citrullinated proteins in arthritis: presence in joints and effects on immunogenicity EP - S7 SN - 1478-6354 SP - S7 JF - Arthritis Research & Therapy VL - vol. 6 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/60098/60098.pdf?sequence=1 ER - TY - JOUR AU - Vossenaar, E.R. AU - Despres, N. AU - Lapointe, E. AU - Heijden, A.G. van der AU - Lora, M. AU - Senshu, T. AU - Venrooij, W.J.W. van AU - Menard, H.A. PY - 2004 UR - https://hdl.handle.net/2066/58941 AB - Antibodies directed to the Sa antigen are highly specific for rheumatoid arthritis ( RA) and can be detected in approximately 40% of RA sera. The antigen, a doublet of protein bands of about 50 kDa, is present in placenta and in RA synovial tissue. Although it has been stated that the Sa antigen is citrullinated vimentin, experimental proof for this claim has never been published. In this study, we investigated the precise nature of the antigen. Peptide sequences that were obtained from highly purified Sa antigen were unique to vimentin. Recombinant vimentin, however, was not recognized by anti-Sa reference sera. In vivo, vimentin is subjected to various post-translational modifications, including citrullination. Since antibodies to citrullinated proteins are known to be highly specific for RA, we investigated whether Sa is citrullinated and found that Sa indeed is citrullinated vimentin. Anti-Sa antibodies thus belong to the family of anticitrullinated protein/peptide antibodies. The presence of the Sa antigen in RA synovial tissue, and the recent observation that vimentin is citrullinated in dying human macrophages, make citrullinated vimentin an interesting candidate autoantigen in RA and may provide new insights into the potential role of citrullinated synovial antigens and the antibodies directed to them in the pathophysiology of RA. TI - Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin EP - R150 SN - 1478-6354 IS - iss. 2 SP - R142 JF - Arthritis Research & Therapy VL - vol. 6 DO - http://dx.doi.org/10.1186/ar1149 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/58941/58941.pdf?sequence=1 ER - TY - JOUR AU - Raijmakers, R. AU - Renz, M. AU - Wiemann, C AU - Egberts, W.V. AU - Seelig, H.P. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2004 UR - https://hdl.handle.net/2066/59043 AB - OBJECTIVE: To compare the autoantigenicity of the recently described N-terminally elongated PM-Scl-75 protein with that of PM-Scl-100 and the originally defined PM-Scl-75 polypeptide, and to determine its value for analyzing sera from patients with the polymyositis (PM)/scleroderma overlap syndrome. METHODS: Serum samples obtained from patients with the PM/scleroderma overlap syndrome and from patients with several other diseases were analyzed for the presence of autoantibodies reactive with recombinant PM-Scl-100 and PM-Scl-75 (both the original and the longer form) proteins, in an enzyme-linked immunosorbent assay (ELISA). RESULTS: Autoantibodies recognizing the longer PM-Scl-75 protein isoform were detected in 28% of the patients with PM/scleroderma. This percentage is slightly higher than that for PM-Scl-100 (25%) and is significantly higher than that for the previously defined PM-Scl-75 protein (11%). In addition, we identified a significant number of patients who had anti-PM-Scl-75 but not anti-PM-Scl-100 antibodies. This finding contrasts with what has been previously reported for the shorter version of the PM-Scl-75 protein. CONCLUSION: Our data indicate that use of the long PM-Scl-75 isoform in addition to PM-Scl-100 in ELISAs significantly increases the number of patients in whom anti-PM-Scl autoantibodies can be detected. TI - PM-Scl-75 is the main autoantigen in patients with the polymyositis/scleroderma overlap syndrome. EP - 569 SN - 0004-3591 IS - iss. 2 SP - 565 JF - Arthritis and Rheumatism VL - vol. 50 DO - http://dx.doi.org/10.1002/art.20056 ER - TY - JOUR AU - Vossenaar, E.R. AU - Radstake, T.R.D.J. AU - Heijden, A.G. van der AU - Mansum, M.A. van AU - Dieteren, C.E.J. AU - Rooij, D.J.R.A.M. de AU - Barrera Rico, P. AU - Zendman, A.J.W. AU - Venrooij, W.J.W. van PY - 2004 UR - https://hdl.handle.net/2066/58497 AB - BACKGROUND: Antibodies directed to proteins containing the non-standard amino acid citrulline, are extremely specific for rheumatoid arthritis (RA). Peptidylcitrulline can be generated by post-translational conversion of arginine residues. This process, citrullination, is catalysed by a group of calcium dependent peptidylarginine deiminase (PAD) enzymes. OBJECTIVE: To investigate the expression and activity of four isotypes of PAD in peripheral blood and synovial fluid cells of patients with RA. RESULTS: The data presented here show that citrullination of proteins by PAD enzymes is a process regulated at three levels: transcription-in peripheral blood PAD2 and PAD4 mRNAs are expressed predominantly in monocytes; PAD4 mRNA is not detectable in macrophages, translation-translation of PAD2 mRNA is subject to differentiation stage-specific regulation by its 3' UTR, and activation-the PAD proteins are only activated when sufficient Ca(2+) is available. Such high Ca(2+) concentrations are normally not present in living cells. In macrophages, which are abundant in the inflamed RA synovium, vimentin is specifically citrullinated after Ca(2+) influx. CONCLUSION: PAD2 and PAD4 are the most likely candidate PAD isotypes for the citrullination of synovial proteins in RA. Our results indicate that citrullinated vimentin is a candidate autoantigen in RA. TI - Expression and activity of citrullinating peptidylarginine deiminase enzymes in monocytes and macrophages. EP - 381 SN - 0003-4967 IS - iss. 4 SP - 373 JF - Annals of the Rheumatic Diseases VL - vol. 63 ER - TY - JOUR AU - Mormann, M. AU - Rieth, H. AU - Hua, T.D. AU - Assohou-Luty, C.A. AU - Roupelieva, M. AU - Hu, S.L. AU - Kremsner, P.G. AU - Luty, J.F. AU - Kube, D. PY - 2004 UR - https://hdl.handle.net/2066/57402 AB - Interleukin-10 (IL-10), a cytokine involved in many aspects of the immune response shows interindividual variations in their expression. However, genetic variations of the 5'-flanking region of the IL-10 gene (PIL-10) are poorly characterised with respect to different stimuli. New extended haplo- and genotypes are identified present at differing frequencies in three geographically separated populations. Their influence on IL-10 expression have been assessed in vitro after stimulation of leukocytes with lipopolysaccharide (LPS), dibutyryl-cAMP or following immortalisation with Epstein-Barr virus (lymphoblastoid cell line (LCL)). Interindividual differences of IL-10 production were found to be related to single-nucleotide polymorphisms (SNP) haplotype -6752/-6208 in LCLs (P<0.02), and for haplotypes comprising SNPs -6752/-6208/-3538 after LPS stimulation (P<0.03). Carriers of the IL10.G microsatellite with 22, 24 or 26 dinucleotide repeats linked with the -1087G SNP, exhibited the highest levels of IL-10 expression. Contrasting IL-10 secretion patterns were found for IL10.R microsatellite alleles characterised by 15 dinucleotide repeats: after LPS stimulation this allele was associated with high IL-10 production (P<0.007), but with low IL-10 levels in LCLs (P< 0.038). Thus, the effects of mosaics of genetic elements in the PIL-10 on the capacity of leukocytes to produce IL-10 depend on the agent inducing IL-10 expression. TI - Mosaics of gene variations in the Interleukin-10 gene promoter affect interleukin-10 production depending on the stimulation used. EP - 255 SN - 1466-4879 IS - iss. 4 SP - 246 JF - Genes and Immunity VL - vol. 5 DO - http://dx.doi.org/10.1038/sj.gene.6364073 ER - TY - JOUR AU - Narayanan, S. AU - Boelens, W.C. AU - Reif, B. PY - 2004 UR - https://hdl.handle.net/2066/57931 TI - Interaction sites between aB crystalline and beta-amyloid as mapped by solution state NMR spectroscopy EP - 76A SN - 0006-3495 IS - iss. 1 SP - 76A JF - Biophysical Journal VL - vol. 86 ER - TY - JOUR AU - Vossenaar, E.R. AU - Depres, N. AU - Lora, M. AU - Heijden, A.G. van der AU - Lapointe, E. AU - Zendman, A.J.W. AU - Senshu, T. AU - Venrooij, W.J.W. van AU - Menard, H.A. PY - 2004 UR - https://hdl.handle.net/2066/57944 TI - The rheumatoid arthritis specific Sa antigen is citrullinated vimentin EP - S8 SN - 1478-6354 IS - iss. suppl. 1 SP - S8 JF - Arthritis Research & Therapy VL - vol. 6 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/57944/57944.pdf?sequence=1 ER - TY - JOUR AU - Rieth, H. AU - Mormann, M. AU - Luty, J.F. AU - Assohou-Luty, C.A. AU - Roupelieva, M. AU - Kremsner, P.G. AU - Kube, D. PY - 2004 UR - https://hdl.handle.net/2066/57578 AB - Interleukin-10 (IL-10) is an important multifunctional immunmodulator. There is evidence that IL-10 secretion is associated with certain genetic elements of the proximal IL-10 gene 5'-flanking region. The allelic and genotypic comparison of IL-10 expression by lipopolysaccharide (LPS)- stimulated leukocytes (PBMC) with a recently discovered distal "indel" DNA-sequence variation at - 7400 bp revealed significant inter-individual differences in the IL-10 in vitro production capacity. Homozygotes lacking the three base pairs "GGA" (- 7400del) at this gene locus are characterised by high expression of IL-10 with a median of 1690pg/ml (P 5' exoribonucleases that functions in a variety of cellular processes, all concerning the processing or degradation of RNA. Paradoxically, the previously described cDNA for the human autoantigenic exosome subunit PM/Scl-75 (Alderuccio, F., Chan, E. K., and Tan, E. M. (1991) J. Exp. Med. 173, 941-952) encodes a polypeptide that failed to interact with the exosome complex. Here, we describe the cloning of a more complete cDNA for PM/Scl-75 encoding 84 additional amino acids at its N terminus. We show that only the longer polypeptide is able to associate with the exosome complex. This interaction is most likely mediated by protein-protein interactions with two other exosome subunits, hRrp46p and hRrp41p, one of which was confirmed in a mammalian two-hybrid system. In addition we show that the putative nuclear localization signal present in the C-terminal region of PM/Scl-75 is sufficient, although not essential for nuclear localization of the protein. Moreover, the deletion of this element abrogated the nucleolar accumulation of PM/Scl-75, although its association with the exosome was not disturbed. This suggests that this basic element of PM/Scl-75 plays a role in targeting the exosome to the nucleolus. TI - The association of the human PM/Scl-75 autoantigen with the exosome is dependent on a newly identified N terminus. EP - 30704 SN - 1083-351X IS - iss. 33 SP - 30698 JF - Journal of Biological Chemistry VL - vol. 278 DO - https://doi.org/10.1074/jbc.M302488200 ER - TY - JOUR AU - Welting, T.J.M. AU - Raijmakers, R. AU - Pruijn, G.J.M. PY - 2003 UR - https://hdl.handle.net/2066/224588 AB - Autoantibodies targeting nucleolar autoantigens (ANoA) are most frequently found in sera from patients with systemic sclerosis (SSc, also designated scleroderma) or with SSc overlap syndromes. During the last decade an extensive number of nucleolar components have been identified and this allowed a more detailed analysis of the identity of nucleolar autoantigens. This review intends to give an overview of the molecular composition of the major (families of) autoantigenic nucleolar complexes, to provide some insight into their functions and to summarise the data concerning their autoantigenicity. TI - Autoantigenicity of nucleolar complexes. EP - 321 SN - 1568-9972 IS - iss. 6 SP - 313 JF - Autoimmunity Reviews VL - vol. 2 DO - https://doi.org/10.1016/S1568-9972(03)00029-6 ER - TY - JOUR AU - Eenennaam, H. van AU - Vogelzangs, J.H.P. AU - Lugtenberg, T.M. AU - Hoogen, F.H.J. van den AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2002 UR - https://hdl.handle.net/2066/138797 AB - OBJECTIVE: To characterize the molecular identity of the Th/To autoantigen, which is targeted by autoantibodies in scleroderma and which is associated with the human RNase MRP and RNase P ribonucleoprotein complexes. METHODS: Proteins immunoprecipitated by anti-Th/To+ patient antisera from biotinylated total HeLa cell extracts were analyzed by immunoblotting. The association of autoantigenic proteins with the RNase MRP complex was analyzed by reconstitution experiments and ultraviolet crosslinking. The reactivity of patient sera with all known RNase MRP/RNase P proteins was analyzed by immunoprecipitation of the individual recombinant proteins. RESULTS: The previously defined Th40 autoantigen appeared to be identical to the Rpp38 protein. Paradoxically, Rpp38 did not bind to the P3 domain of the RNase MRP RNA, as suggested by previously published data for Th40, and only half of the anti-Th/To+ sera contained anti-Rpp38 reactivity. Two other RNase MRP/RNase P subunits, Rpp20 and Rpp25, were found to interact with the P3 domain. The previously reported 40-kd species associated with this domain appeared to consist of Rpp20 and/or Rpp25 associated with a nuclease-resistant RNA fragment. Finally, we demonstrated that almost all tested anti-Th/To+ patient sera contained autoantibodies to Rpp25 and hPop1, indicating that these proteins harbor the most frequently targeted Th/To determinants. CONCLUSION: Our data unequivocally define the identity of the Th/To autoantigen and demonstrate that Th/To autoepitopes are found on several protein subunits of RNase MRP/RNase P. TI - Identity of the RNase MRP- and RNase P-associated Th/To autoantigen. EP - 3272 SN - 0004-3591 IS - iss. 12 SP - 3266 JF - Arthritis and Rheumatism VL - vol. 46 DO - http://dx.doi.org/10.1002/art.10673 ER - TY - JOUR AU - Raijmakers, R. AU - Egberts, W.V. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2002 UR - https://hdl.handle.net/2066/186665 AB - The exosome is a complex of 3'-->5' exoribonucleases, which functions in a variety of cellular processes, all requiring the processing or degradation of RNA. Here we present a model for the assembly of the six human RNase PH-like exosome subunits into a hexameric ring structure. In part, this structure is on the basis of the evolutionarily related bacterial degradosome, the core of which consists of three copies of the PNPase protein, each containing two RNase PH domains. In our model three additional exosome subunits, which contain S1 RNA-binding domains, are positioned on the outer surface of this ring. Evidence for this model was obtained by the identification of protein-protein interactions between individual exosome subunits in a mammalian two-hybrid system. In addition, the results of co-immunoprecipitation assays indicate that at least two copies of hRrp4p and hRrp41p are associated with a single exosome, suggesting that at least two of these ring structures are present in this complex. Finally, the identification of a human gene encoding the putative human counterpart of the bacterial PNPase protein is described, which suggests that the exosome is not the eukaryotic equivalent of the bacterial degradosome, although they do share similar functional activities. TI - Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring. EP - 663 SN - 0022-2836 IS - iss. 4 SP - 653 JF - Journal of Molecular Biology VL - vol. 323 DO - http://dx.doi.org/10.1016/S0022-2836(02)00947-6 ER - TY - JOUR AU - Malmegrim de Farias, K.C. AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van PY - 2002 UR - https://hdl.handle.net/2066/187317 AB - Recent studies have implicated the dying cell as a potential reservoir of modified autoantigens that may initiate and drive systemic autoimmunity in susceptible hosts. The uridine-rich small nuclear ribonucleoprotein complex is a common target for autoantibodies present in the serum of patients with systemic lupus erythematosus and SLE-overlap syndromes. Four modifications occurring in this complex during apoptosis have been described to date: the caspase-mediated cleavage of the U1-70K protein, the U1 RNA and the Sm-F protein, and the association with hyperphosphorylated SR proteins. In addition, the U1 snRNP complex has been shown to translocate from its normal subcellular localization to apoptotic bodies near the surface of cells undergoing apoptosis. This redistribution might facilitate exposure of the modified components of the U1 snRNP complex to the immune system when the clearance of apoptotic cell remnants is somehow disturbed. The modifications in the U1 snRNP components during apoptosis might represent the initial epitopes to which an immune response is generated and may be the trigger for the production of autoantibodies to this complex in patients with SLE or SLE-overlap syndromes. Therefore, it can be hypothesized that the exposure of elevated levels of apoptotically modified U1 snRNP to the immune system of a genetically susceptible individual might lead to the breaking of immunologic tolerance towards the U1 snRNP complex. TI - The fate of the U1 snRNP autoantigen during apoptosis: implications for systemic autoimmunity. EP - 712 SN - 1565-1088 IS - iss. 9 SP - 706 JF - Imaj VL - vol. 4 ER - TY - JOUR AU - Robinson, W.H. AU - DiGennaro, C. AU - Hueber, W. AU - Haab, B.B. AU - Kamachi, M. AU - Dean, E.J. AU - Fournel, S. AU - Fong, D. AU - Genovese, M.C. AU - Vegvar, H.E. de AU - Skriner, K. AU - Hirschberg, D.L. AU - Morris, R.I. AU - Muller, S. AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van AU - Smolen, J.S. AU - Brown, P. AU - Steinman, L. AU - Utz, P.J. PY - 2002 UR - https://hdl.handle.net/2066/176280 AB - We constructed miniaturized autoantigen arrays to perform large-scale multiplex characterization of autoantibody responses directed against structurally diverse autoantigens, using submicroliter quantities of clinical samples. Autoantigen microarrays were produced by attaching hundreds of proteins, peptides and other biomolecules to the surface of derivatized glass slides using a robotic arrayer. Arrays were incubated with patient serum, and spectrally resolvable fluorescent labels were used to detect autoantibody binding to specific autoantigens on the array. We describe and characterize arrays containing the major autoantigens in eight distinct human autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. This represents the first report of application of such technology to multiple human disease sera, and will enable validated detection of antibodies recognizing autoantigens including proteins, peptides, enzyme complexes, ribonucleoprotein complexes, DNA and post-translationally modified antigens. Autoantigen microarrays represent a powerful tool to study the specificity and pathogenesis of autoantibody responses, and to identify and define relevant autoantigens in human autoimmune diseases. TI - Autoantigen microarrays for multiplex characterization of autoantibody responses. EP - 301 SN - 1078-8956 IS - iss. 3 SP - 295 JF - Nature Medicine VL - vol. 8 DO - https://doi.org/10.1038/nm0302-295 ER - TY - JOUR AU - Eenennaam, H. van AU - Vogelzangs, J.H.P. AU - Bisschops, L.L.A. AU - Boome, L.C. te AU - Seelig, H.P. AU - Renz, M. AU - Brouwer, R. AU - Pluk, W.L.L.P. AU - Hoogen, F.H.J. van den AU - Rooij, D.J.R.A.M. de AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van PY - 2002 UR - https://hdl.handle.net/2066/138796 AB - Sera from patients suffering from systemic autoimmune diseases such as systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) have been shown to contain reactivities to nuclear components. Autoantibodies specifically targeting nucleolar antigens are found most frequently in patients suffering from SSc or SSc overlap syndromes. We determined the prevalence and clinical significance of autoantibodies directed to nucleolar RNA-protein complexes, the so-called small nucleolar ribonucleoprotein complexes (snoRNPs). A total of 172 patient sera with antinucleolar antibodies were analysed by immunoprecipitation. From 100 of these patients clinical information was obtained by chart review. Autoantibodies directed to snoRNPs were detected not only in patients suffering from SSc and primary Raynaud's phenomenon (RP), but also in patients suffering from SLE, rheumatoid arthritis (RA) and myositis (PM/DM). Antibodies against box C/D small snoRNPs can be subdivided in antifibrillarin positive and antifibrillarin negative reactivity. Antifibrillarin-positive patient sera were associated with a poor prognosis in comparison with antifibrillarin negative (reactivity with U3 or U8 snoRNP only) patient sera. Anti-Th/To autoantibodies were associated with SSc, primary RP and SLE and were found predominantly in patients suffering from decreased co-diffusion and oesophagus motility and xerophthalmia. For the first time autoantibodies that recognize box H/ACA snoRNPs are described, identifying this class of snoRNPs as a novel autoantigenic activity. Taken together, our data show that antinucleolar patient sera directed to small nucleolar ribonucleoprotein complexes are found frequently in other diseases than SSc and that categorization of diagnoses and clinical manifestations based on autoantibody profiles seems particularly informative in patient sera recognizing box C/D snoRNPs. TI - Autoantibodies against small nucleolar ribonucleoprotein complexes and their clinical associations. EP - 540 SN - 0009-9104 IS - iss. 3 SP - 532 JF - Clinical and Experimental Immunology VL - vol. 130 DO - https://doi.org/10.1046/j.1365-2249.2002.01991.x ER - TY - JOUR AU - Zendman, A.J.W. AU - Kraats, A.A. van AU - Hollander, A.I. den AU - Weidle, U.H. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 2002 UR - https://hdl.handle.net/2066/134191 AB - Suppression subtractive hybridization, comparing mRNA expression profiles of common nevocellular nevi and melanoma metastases, was used to identify potential markers of melanoma progression. From the metastases we isolated XAGE-1b, a 470 bp transcript of the XAGE-1 gene. In general, expression of XAGE-1b was much more prominent than expression of the longer XAGE-1 transcript, isolated from Ewing's sarcoma. The XAGE-1b open-reading frame codes for a putative protein of 81 amino acids, harboring a functional bipartite nuclear localization signal and a C-terminal acidic transcription-activation-like domain. On the nucleotide level, XAGE-1b has a 50% homology with members of the GAGE family. However, homology between the corresponding proteins is weak. Expression of XAGE-1b in normal tissues was mainly restricted to testis, while placenta and brain were sporadically positive. In human tumor cell lines as well as in human tumor lesions, expression was most frequently found in melanocytic tumors and Ewing's sarcoma. In the different stages of melanocytic tumor progression, expression was exclusively seen in melanoma metastases (38%; n = 61), while all tested common and atypical nevi (n = 10) as well as primary melanomas (n = 8) were negative. Upregulation of expression after treatment with demethylating agent 5-aza-2'-deoxycytidine was detected in 1 of 4 human melanoma cell lines tested. The XAGE-1 gene consists of 4 exons and is located on chromosome Xp11.21-Xp11.22. After transfection into COS cells, the corresponding protein can direct the coupled fluorescent protein to the nucleus, showing a distinct speckled staining aspect. Our data imply the nuclear cancer/testis-associated XAGE-1b to be a marker for late melanocytic tumor progression. TI - Characterization of XAGE-1b, a short major transcript of cancer/testis-associated gene XAGE-1, induced in melanoma metastasis. EP - 204 SN - 0020-7136 IS - iss. 2 SP - 195 JF - International Journal of Cancer VL - vol. 97 DO - https://doi.org/10.1002/ijc.1584 ER - TY - JOUR AU - Leenders, W.P.J. AU - Lubsen, N.H. AU - Altena, M.C. van AU - Clauss, M. AU - Deckers, M. AU - Lowik, C.W.G.M. AU - Breier, G. AU - Ruiter, D.J. AU - Waal, R.M.W. de PY - 2002 UR - https://hdl.handle.net/2066/121082 AB - Because of its central role in pathological angiogenesis, vascular endothelial growth factor (VEGF) has become a major target for anti-angiogenic therapies. We report here the construction of a heterodimeric antagonistic VEGF variant (HD-VEGF). In this antagonist, binding domains for the VEGF-receptors KDR/Flk-1 and Flt-1 are present at one pole of the dimer, whereas the other pole carries domain swap mutations, which prevent binding to either receptor. As HD-VEGF can only bind to monomeric receptors, it does not lead to signal transduction. Moreover, it antagonizes VEGF and possibly other members of the VEGF family, which are KDR/Flk-1 and Flt-1 ligands. We show here that HD-VEGF is a potent inhibitor of VEGF-mediated proliferation and tissue factor induction in endothelial cell cultures, requiring only a 20-fold and a 4-fold excess, respectively, to block the activity of wtVEGF completely. A 4-fold excess of HD-VEGF over wtVEGF was also sufficient to abrogate vascular permeability as determined in the Miles assay in vivo. Furthermore, HD-VEGF inhibited fetal bone angiogenesis in an ex vivo assay. Thus, HD-VEGF blocks KDR- and Flt-1-mediated VEGF activities that are crucial in the angiogenic process and is therefore a promising, multipotent compound in the treatment of angiogenesis-related diseases. TI - Design of a variant of vascular endothelial growth factor-A (VEGF-A) antagonizing KDR/Flk-1 and Flt-1. EP - 481 SN - 0023-6837 IS - iss. 4 SP - 473 JF - Laboratory Investigation VL - vol. 82 DO - https://doi.org/10.1038/labinvest.3780440 ER - TY - JOUR AU - Fouraux, M.A. AU - Bouvet, P. AU - Verkaart, S.A.J. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2002 UR - https://hdl.handle.net/2066/121576 AB - Ro RNPs are evolutionarily conserved, small cytoplasmic RNA-protein complexes with an unknown function. In human cells, Ro RNPs consist of one of the four hY RNAs and two core proteins: Ro60 and La. Recently, the association of hnRNP I and hnRNP K with particles containing hY1 and hY3 RNAs has been described. The association of three other proteins, namely calreticulin, Ro52 and RoBPI, with (subsets of) the Ro RNPs is still controversial. To gain more insight into the composition and function of the Ro RNPs, we have immunopurified these particles from HeLa cell extracts using monoclonal antibodies against Ro60 and La. Using this approach, we have identified the RNA-binding protein nucleolin as a novel subunit of Ro RNP particles containing hY1 or hY3 RNA, but not hY4 and hY5 RNA. Using an in vitro hY RNA-binding assay we established that the internal pyrimidine-rich loop of hY1 and hY3 RNA is essential for the association of nucleolin. The binding is critically dependent on the presence of all four RNP motifs of nucleolin, but not of the C-terminal RGG-box. Moreover, we demonstrate that, in contrast to nucleolin and hnRNP K, nucleolin and hnRNP I can bind simultaneously to the internal pyrimidine-rich loop of hY1 RNA. We postulate that nucleolin functions in the biogenesis and/or trafficking of hY1 and hY3 RNPs through the nucleolus and subsequent transport to the cytoplasm. TI - Nucleolin associates with a subset of the human Ro ribonucleoprotein complexes. EP - 488 SN - 0022-2836 IS - iss. 3 SP - 475 JF - Journal of Molecular Biology VL - vol. 320 DO - https://doi.org/10.1016/S0022-2836(02)00518-1 ER - TY - JOUR AU - Brouwer, R. AU - Vree Egberts, W.T.M. AU - Hengstman, G.J.D. AU - Raijmakers, R. AU - Engelen, B.G.M. van AU - Seelig, H.P. AU - Renz, M. AU - Mierau, R. AU - Genth, E. AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van PY - 2002 UR - https://hdl.handle.net/2066/186494 AB - The autoantigenic polymyositis/scleroderma (PM/Scl) complex was recently shown to be the human homologue of the yeast exosome, which is an RNA-processing complex. Our aim was to assess whether, in addition to targeting the known autoantigens PM/Scl-100 and PM/Scl-75, autoantibodies also target recently identified components of the PM/Scl complex. The prevalence of autoantibodies directed to six novel human exosome components (hRrp4p, hRrp40p, hRrp41p, hRrp42p, hRrp46p, hCsl4p) was determined in sera from patients with idiopathic inflammatory myopathy (n = 48), scleroderma (n = 11), or the PM/Scl overlap syndrome (n = 10). The sera were analyzed by enzyme-linked immunosorbent assays and western blotting using the affinity-purified recombinant proteins. Our results show that each human exosome component is recognized by autoantibodies. The hRrp4p and hRrp42p components were most frequently targeted. The presence of autoantibodies directed to the novel components of the human exosome was correlated with the presence of the anti-PM/Scl-100 autoantibody in the sera of patients with idiopathic inflammatory myopathy (IIM), as was previously found for the anti-PM/Scl-75 autoantibody. Other clear associations between autoantibody activities were not found. These results further support the conception that the autoimmune response may initially be directed to PM/Scl-100, whereas intermolecular epitope spreading may have caused the autoantibody response directed to the associated components. TI - Autoantibodies directed to novel components of the PM/Scl complex, the human exosome. EP - 138 SN - 1478-6354 IS - iss. 2 SP - 134 JF - Arthritis Research & Therapy VL - vol. 4 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/186494/186494.pdf?sequence=1 ER - TY - JOUR AU - Zendman, A.J.W. AU - Kraats, A.A. van AU - Weidle, U.H. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 2002 UR - https://hdl.handle.net/2066/187661 AB - The existence of XAGE genes was first reported after database homology searches for PAGE-like sequences identified 3 XAGE EST clusters. One of these clusters, XAGE-1, has in later studies been identified as a cancer/testis-associated gene. Here, we report the expression profiles of all 3 reported XAGE genes, as well as several splice variants of XAGE-1, in normal human tissues, Ewing's sarcoma and melanocytic tumors. We also provide the genetic structure of the corresponding genes. Moreover, by searching the databases for XAGE homologues, we identified 3 additional GAGE-like genes. RT-PCR studies showed frequent expression in melanoma metastases and Ewing's sarcoma for 2 XAGE-1-derived transcripts. XAGE-2 was expressed at lower frequency in these tissues, while XAGE-3 was seen only in normal placenta. Due to a frameshift, the largest XAGE-1 putative protein is far less homologous to GAGE-like proteins than the other XAGEs. Interestingly, all GAGE-like genes contain a large secondary open reading frame, coding for putative proteins homologues to the XAGE-1 primary protein. The XAGE family of cancer/testis-associated genes is located on chromosome Xp11.21-Xp11.22. The data outline a superfamily of GAGE-like cancer/testis antigens, consisting of at least 19 genes. TI - The XAGE family of cancer/testis-associated genes: alignment and expression profile in normal tissues, melanoma lesions and Ewing's sarcoma. EP - 369 SN - 0020-7136 IS - iss. 3 SP - 361 JF - International Journal of Cancer VL - vol. 99 DO - https://doi.org/10.1002/ijc.10371 ER - TY - JOUR AU - Granneman, S. AU - Pruijn, G.J.M. AU - Horstman, W.A.M. AU - Venrooij, W.J.W. van AU - Luhrmann, R AU - Watkins, N.J. PY - 2002 UR - https://hdl.handle.net/2066/185501 AB - The 15.5K protein directly binds to the 5' stem-loop of the U4 small nuclear RNA, the small nucleolar (sno) RNA box C/D motif, and the U3 snoRNA-specific box B/C motif. The box B/C motif has also been shown to be essential for the association of the U3 small nucleolar ribonucleoprotein-specific protein hU3-55K. We therefore set out to determine how 15.5K and hU3-55K recognize the box B/C motif. By using an in vitro assembly assay, we show that hU3-55K effectively binds a sub-fragment of the U3 snoRNA surrounding the B/C motif that we have named the U3BC RNA. The association of hU3-55K with the U3BC RNA is dependent on the binding of 15.5K to the box B/C motif. The association of hU3-55K with the U3BC RNA was found to be also dependent on a conserved RNA structure that flanks the box B/C motif. Furthermore, we show that hU3-55K, a WD 40 repeat containing protein, directly cross-links to the U3BC RNA. Our data support a new structural model of the box B/C region of the U3 snoRNA in which the box B/C motif is base-paired to form a structure highly similar to that of both the U4 5' stem-loop and the box C/D motif. TI - The hU3-55K protein requires 15.5K binding to the box B/C motif as well as flanking RNA elements for its association with the U3 small nucleolar RNA in Vitro. EP - 48500 SN - 1083-351X IS - iss. 50 SP - 48490 JF - Journal of Biological Chemistry VL - vol. 277 DO - https://doi.org/10.1074/jbc.M206631200 ER - TY - JOUR AU - Peek, R. AU - Kammerer, R.A. AU - Frank, S. AU - Otte-Holler, I. AU - Westphal, J.R. PY - 2002 UR - https://hdl.handle.net/2066/121088 AB - The human cornea-specific protein cornea-derived transcript 6 (CDT6) is a member of the angiopoietin gene family. We report the structural and functional characterization of CDT6. We demonstrate that CDT6 is a secreted protein that folds into disulfide-linked homotetramers by coiled-coil interactions. The finding that CDT6 is expressed at high levels in the avascular corneal stromal layer suggested that the protein, similar to certain angiopoietins, acts as a negative regulator of angiogenesis. To test this hypothesis and to assay the effect of the protein on a growing tissue with high vascular density, CDT6 was expressed in a mouse xenograft model. Expression of CDT6 led to a reduction in tumor growth and aberrant blood vessel formation by inducing massive fibrosis. Interestingly, expression of CDT6 also resulted in the deposition of extracellular matrix components typical for the mature corneal stromal layer. These observations strongly suggest a role as morphogen for CDT6 in inducing a corneal phenotype in vivo. TI - The angiopoietin-like factor cornea-derived transcript 6 is a putative morphogen for human cornea. EP - 693 SN - 1083-351X IS - iss. 1 SP - 686 JF - Journal of Biological Chemistry VL - vol. 277 DO - https://doi.org/10.1074/jbc.M105746200 ER - TY - JOUR AU - Debeer, P. AU - Schoenmakers, E.F.P.M. AU - Twal, W.O. AU - Argraves, W.S. AU - Smet, L. de AU - Fryns, J.P. AU - Ven, W.J.M. van de PY - 2002 UR - https://hdl.handle.net/2066/186697 AB - Molecular analysis of the reciprocal chromosomal translocation t(12;22)(p11.2;q13.3) cosegregating with a complex type of synpolydactyly showed involvement of an alternatively spliced exon of the fibulin-1 gene (FBLN1 located in 22q13.3) and the C12orf2 (HoJ-1) gene on the short arm of chromosome 12. Investigation of the possible functional involvement of the fibulin-1 protein (FBLN1) in the observed phenotype showed that FBLN1 is expressed in the extracellular matrix (ECM) in association with the digits in the developing limb. Furthermore, fibroblasts derived from patients with the complex type of synpolydactyly displayed alterations in the level of FBLN1-D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of the FBLN1-C splice variant was not perturbed in the patient fibroblasts. Based on these findings, we propose that the t(12;22) results in haploinsufficiency of the FBLN1-D variant, which could lead to the observed limb malformations. TI - The fibulin-1 gene (FBLN1) is disrupted in a t(12;22) associated with a complex type of synpolydactyly. EP - 104 SN - 0022-2593 IS - iss. 2 SP - 98 JF - Journal of Medical Genetics VL - vol. 39 DO - https://doi.org/10.1136/jmg.39.2.98 ER - TY - JOUR AU - Klundert, F.A.J.M. van de AU - Gijsen, M.L.J. AU - IJssel, P.R.L.A. van den AU - Snoeckx, L.H. AU - Jong, W.W.W. de PY - 2002 UR - https://hdl.handle.net/2066/187662 TI - aB-crystallin and hsp25 in neonatal cardiac cells - Differences in cellular localization under stress conditions. EP - 45 SN - 0171-9335 SP - 38 JF - European Journal of Cell Biology VL - vol. 75 DO - https://doi.org/10.1016/S0171-9335(98)80044-7 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Brouwer, R. AU - Vree Egberts, W.T.M. AU - Seelig, H.P. AU - Jongen, P.J.H. AU - Venrooij, W.J.W. van AU - Engelen, B.G.M. van PY - 2002 UR - https://hdl.handle.net/2066/186508 AB - The idiopathic inflammatory myopathies (IIM) are a heterogeneous group of systemic diseases that include the familiar disease entities of dermatomyositis (DM), polymyositis (PM), and inclusion body myositis (IBM). A subset of patients has unique autoantibodies which are specific for IIM (myositis specific autoantibodies; MSAs). We studied the clinical and serological characteristics of IIM in 125 Dutch patients. Sera were analysed by immunoblotting, enzyme-linked immunosorbent assay, and immunoprecipitation. The most frequently encountered MSA was the anti-Jo-1 autoantibody (20%), followed by anti-tRNAHis (6%), anti-Mi-2 (6%), and anti-SRP (4%). The presence of certain MSAs was clearly associated with specific clinical characteristics. Anti-Jo-1 and anti-tRNAHis were associated with the anti-synthetase syndrome, anti-SRP with PM with severe myalgia and arthralgia and a moderate response to immunosuppressive treatment. A novel finding was the presence of anti-Mi-2, not only in DM, but also in PM. MSAs were frequently present in DM/PM sera, but were hardly ever detected in the sera of IBM patients. The few IBM patients with MSAs demonstrated a significant response to immunosuppressive treatment. It can be concluded that MSAs define specific clinical syndromes within the spectrum of IIM and that they can assist in the differential diagnosis and treatment plan of these enigmatic disorders by virtually excluding IBM by their presence, and by potentially identifying a subgroup of steroid-responsive IBM patients. TI - Clinical and serological characteristics of 125 Dutch myositis patients. Myositis specific autoantibodies aid in the differential diagnosis of the idiopathic inflammatory myopathies. EP - 75 SN - 0340-5354 IS - iss. 1 SP - 69 JF - Journal of Neurology VL - vol. 249 DO - https://doi.org/10.1007/PL00007850 ER - TY - JOUR AU - Kempen, L.C.L.T. van AU - Nelissen, J.M.D.T. AU - Degen, W.G.J. AU - Torensma, R. AU - Weidle, U.H. AU - Bloemers, H.P.J. AU - Figdor, C.G. AU - Swart, G.W.M. PY - 2001 UR - https://hdl.handle.net/2066/27305 PB - Amer soc biochemistry molecular biology inc TI - Molecular basis for the hemophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction EP - 25790 SN - 0021-9258 IS - iss. 28 SP - 25783 JF - Journal of Biological Chemistry VL - vol. 276 ER - TY - JOUR AU - Eenennaam, H. van AU - Heijden, A.G. van der AU - Janssen, R.J.T. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2001 UR - https://hdl.handle.net/2066/143784 AB - The RNase MRP and RNase P ribonucleoprotein particles both function as endoribonucleases, have a similar RNA component, and share several protein subunits. RNase MRP has been implicated in pre-rRNA processing and mitochondrial DNA replication, whereas RNase P functions in pre-tRNA processing. Both RNase MRP and RNase P accumulate in the nucleolus of eukaryotic cells. In this report we show that for three protein subunits of the RNase MRP complex (hPop1, hPop4, and Rpp38) basic domains are responsible for their nucleolar accumulation and that they are able to accumulate in the nucleolus independently of their association with the RNase MRP and RNase P complexes. We also show that certain mutants of hPop4 accumulate in the Cajal bodies, suggesting that hPop4 traverses through these bodies to the nucleolus. Furthermore, we characterized a deletion mutant of Rpp38 that preferentially associates with the RNase MRP complex, giving a first clue about the difference in protein composition of the human RNase MRP and RNase P complexes. On the basis of all available data on nucleolar localization sequences, we hypothesize that nucleolar accumulation of proteins containing basic domains proceeds by diffusion and retention rather than by an active transport process. The existence of nucleolar localization sequences is discussed. TI - Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association. EP - 3689 SN - 1059-1524 IS - iss. 11 SP - 3680 JF - Molecular Biology of the Cell VL - vol. 12 DO - http://dx.doi.org/10.1091/mbc.12.11.3680 ER - TY - JOUR AU - Rutjes, S.A. AU - Lund, E. AU - Heijden, A.G. van der AU - Grimm, C AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2001 UR - https://hdl.handle.net/2066/143797 AB - Ro RNPs are small cytoplasmic RNA-protein complexes of unknown function that have been found in all metazoan cells studied so far. In human cells, Ro RNPs consist of one of four small RNA molecules, termed hY RNAs and at least two well-characterized proteins, Ro60 and La. In previous Xenopus laevis oocyte microinjection studies, we showed that an intact Ro60 binding site (Stem-loop 1) is a prerequisite for efficient nuclear export of hY1 RNA, whereas an intact La-binding site promotes nuclear retention (Simons et al. RNA, 1996, 2:264-273). Here we present evidence that the distal half (Stem 2) of the conserved base-paired stem structure found in all hY RNAs also plays a critical role in the export process. A minimal RNA molecule containing this region, L1S2 RNA, competes effectively for the export of full-length hY1 RNAs and is itself exported very rapidly in a Ro60-independent and RanGTP-dependent manner. Mutational analyses of this RNA shows that a 5'/3' terminal double-stranded stem structure (>10 bp) of no specific nucleotide sequence constitutes a novel nuclear export element (NEE). Cross-competition studies indicate that this type of NEE may also be involved in export of other classes of RNAs. Like full-length hY1 RNA, L1S2 RNA also competes for export of ET-202 RNA, an RNA that was selected for its efficient nuclear export in the presence of the nuclear transport inhibitor, VSV Matrix protein (Grimm et al. Proc Natl Acad Sci USA, 1997, 94:10122-10127). However, export of L1S2 RNA is strongly inhibited by VSV-M protein, showing that these RNAs use partially overlapping, but not identical export pathways. We propose that export of Y RNAs is mediated by two contiguous cis-acting elements in the 5'/3' double-stranded stem region that is conserved between different Y RNAs. TI - Identification of a novel cis-acting RNA element involved in nuclear export of hY RNAs. EP - 752 SN - 1355-8382 IS - iss. 5 SP - 741 JF - Rna : a Publication of the Rna Society VL - vol. 7 DO - http://dx.doi.org/10.1017/S1355838201002503 ER - TY - JOUR AU - Ridanpaa, M AU - Eenennaam, H. van AU - Pelin, K. AU - Chadwick, R. AU - Johnson, C AU - Yuan, B. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. AU - Salmela, R. AU - Rockas, S. AU - Makitie, O. AU - Tollervey, D. AU - Chapelle, A. dela PY - 2001 UR - https://hdl.handle.net/2066/185709 AB - The recessively inherited developmental disorder, cartilage-hair hypoplasia (CHH) is highly pleiotropic with manifestations including short stature, defective cellular immunity, and predisposition to several cancers. The endoribonuclease RNase MRP consists of an RNA molecule bound to several proteins. It has at least two functions, namely, cleavage of RNA in mitochondrial DNA synthesis and nucleolar cleaving of pre-rRNA. We describe numerous mutations in the untranslated RMRP gene that cosegregate with the CHH phenotype. Insertion mutations immediately upstream of the coding sequence silence transcription while mutations in the transcribed region do not. The association of protein subunits with RNA appears unaltered. We conclude that mutations in RMRP cause CHH by disrupting a function of RNase MRP RNA that affects multiple organ systems. TI - Mutations in the RNA component of RNase MRP cause a pleiotropic human disease, cartilage-hair hypoplasia. EP - 203 SN - 0092-8674 IS - iss. 2 SP - 195 JF - Cell VL - vol. 104 DO - https://doi.org/10.1016/S0092-8674(01)00205-7 ER - TY - JOUR AU - Zendman, A.J.W. AU - Wit, N.J. de AU - Kraats, A.A. van AU - Weidle, U.H. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 2001 UR - https://hdl.handle.net/2066/187663 AB - Vaccination-based therapy of melanoma has so far mainly focused on monovalent approaches using either melanoma differentiation antigens or cancer/testis antigens. To study the complementarity of expression from these two families of antigens recognized by T-cells, we screened 47 metastatic lesions of cutaneous melanoma for the expression of three melanoma differentiation antigens and eight cancer/testis antigens using reverse transcription-polymerase chain reaction (RT-PCR). The melanoma differentiation antigens were expressed in a somewhat higher percentage of lesions (94% positive for at least one marker) than the cancer/testis antigens (91% positive for at least one marker). Nearly all the melanoma metastases (98%) expressed at least one of the markers tested. One melanoma metastasis was negative for all the markers. Two out of 47 lesions did not express any of the three differentiation markers but expressed one or more of the cancer/testis antigens, indicating some additional potential for these antigens compared with the melanoma differentiation antigens. Therefore, we conclude that polyvalent immunotherapy using multiple epitopes from both families of antigens might increase the eligibility of melanoma patients and the efficacy of the treatment. TI - Expression profile of genes coding for melanoma differentiation antigens and cancer/testis antigens in metastatic lesions of human cutaneous melanoma. EP - 9 SN - 0960-8931 IS - iss. 5 SP - 451- JF - Melanoma Research VL - vol. 11 DO - https://doi.org/10.1097/00008390-200110000-00003 ER - TY - JOUR AU - Seidl, R. AU - Labudova, O. AU - Krapfenbauer, K. AU - Henriksson, E.W. AU - Craft, J AU - Turhani-Schatzmann, D. AU - Achsel, T AU - Bidmon, B AU - Pruijn, G.J.M. AU - Cairns, N AU - Lubec, G PY - 2001 UR - https://hdl.handle.net/2066/185604 AB - The small nuclear ribonucleoprotein 70K (snRNP 70K; U1-70 kDa) is an integral part of the spliceosome, a large RNA-protein complex catalyzing the removal of introns from nuclear pre-mRNA. snRNP is one of the best-studied essential subunits of snRNPs, is highly conserved and its inactivation was shown to result in complete inhibition of splicing. Applying subtractive hybridization, we found a sequence with 100% identity to snRNP absent in fetal Down syndrome (DS) brain. This observation made us determine snRNP-mRNA steady-state levels and protein levels in brains of adult patients with DS. snRNP-mRNA and protein levels of five individual brain regions of DS and controls each, were determined by blotting techniques. snRNP-mRNA steady state levels were significantly decreased in DS brain. Performing Western blots with monoclonal and human antibodies, snRNP protein levels were decreased in several regions of DS brain, although one monoclonal antibody did not reveal different snRNP-immunoreactivity. Although decreased snRNP-protein could be explained by decreased mRNA-steady state levels, another underlying mechanism might be suggested: snRNP is one of the death substrates rapidly cleaved during apoptosis by interleukin-1-beta-converting enzyme-like (ICE) proteases, which was well-documented by several groups. As apoptosis is unrequivocally taking place in DS brain leading to permanent cell loses, decreased snRNP-protein levels may therefore reflect decreased synthesis and increased apoptosis-related proteolytic cleavage. TI - Deficient brain snRNP70K in patients with Down syndrome. EP - 48 SN - 0173-0835 IS - iss. 1 SP - 43 JF - Electrophoresis VL - vol. 22 DO - https://doi.org/10.1002/1522-2683(200101)22:1<43 ER - TY - JOUR AU - Chen, C.Y. AU - Gherzi, R AU - Ong, S.E. AU - Chan, E.L. AU - Raijmakers, R. AU - Pruijn, G.J.M. AU - Stoecklin, G. AU - Moroni, C. AU - Mann, M. AU - Karin, M PY - 2001 UR - https://hdl.handle.net/2066/185554 AB - Inherently unstable mammalian mRNAs contain AU-rich elements (AREs) within their 3' untranslated regions. Although found 15 years ago, the mechanism by which AREs dictate rapid mRNA decay is not clear. In yeast, 3'-to-5' mRNA degradation is mediated by the exosome, a multisubunit particle. We have purified and characterized the human exosome by mass spectrometry and found its composition to be similar to its yeast counterpart. Using a cell-free RNA decay system, we demonstrate that the mammalian exosome is required for rapid degradation of ARE-containing RNAs but not for poly(A) shortening. The mammalian exosome does not recognize ARE-containing RNAs on its own. ARE recognition requires certain ARE binding proteins that can interact with the exosome and recruit it to unstable RNAs, thereby promoting their rapid degradation. TI - AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. EP - 464 SN - 0092-8674 IS - iss. 4 SP - 451 JF - Cell VL - vol. 107 DO - https://doi.org/10.1016/S0092-8674(01)00578-5 ER - TY - JOUR AU - Sowa, K.M. AU - Cavanagh, D R AU - Creasey, A.M. AU - Raats, J.M.H. AU - McBride, J. AU - Sauerwein, R.W. AU - Roeffen, W.F.G. AU - Arnot, D. PY - 2001 UR - https://hdl.handle.net/2066/186845 TI - Isolation of a monoclonal antibody from a malaria patient-derived phage display library recognising the Block 2 region of Plasmodium falciparum merozoite surface protein-1. EP - 7 SN - 0166-6851 IS - iss. 1 SP - 143- JF - Molecular and Biochemical Parasitology VL - vol. 112 DO - https://doi.org/10.1016/S0166-6851(00)00348-0 ER - TY - JOUR AU - Leenders, W.P.J. AU - Altena, M.C. van AU - Lubsen, N.H. AU - Ruiter, D.J. AU - Waal, R.M.W. de PY - 2001 UR - https://hdl.handle.net/2066/121092 AB - VEGF mutants in which Cys51 or Cys60 are converted into a serine are poor inducers of proliferation in human umbilical vein endothelial cells, but they have wild-type activity in the Miles vascular permeability assay. To assess the contribution of proliferation vs. other VEGF activities such as vascular permeability, to tumor angiogenesis and growth, C127I cells, transfected with BPV-based expression plasmids carrying wild-type or mutated VEGF cDNAs, were injected subcutaneously in BALB/c nu/nu mice. From C127I cells expressing wtVEGF(165), intensely vascularized and invasive tumors developed within 2 to 3 weeks. From cells expressing VEGF-Cys51Ser or VEGF-Cys60Ser, tumors developed only after 2 to 3 months, comparable to the time of development of control tumors, i.e., tumors from cells transfected with empty vector. Despite the late take, the VEGF-Cys51Ser and VEGF-Cys60Ser tumors developed an extensive vascular bed with an architecture comparable to that of recombinant wtVEGF-producing tumors whereas control tumors had a considerably lower vascular density. No metastases were detected in mice carrying either wtVEGF or mutant VEGF expressing tumors. Thus, because proliferation-defective VEGF-mutants cannot induce angiogenesis, we conclude that the proliferation-inducing effect of VEGF is crucial for tumor angiogenesis and growth. The hypervasculature in the tumors expressing these VEGF-mutants suggests, however, that other VEGF-activities, such as the induction of vascular permeability, strongly affects vascular density and vascular structure. Furthermore, neither overexpression of VEGF or a high vascular density or hyperpermeability of tumor vasculature is necessarily followed by metastasis. TI - In vivo activities of mutants of vascular endothelial growth factor (VEGF) with differential in vitro activities. EP - 33 SN - 0020-7136 IS - iss. 3 SP - 327- JF - International Journal of Cancer VL - vol. 91 DO - https://doi.org/10.1002/1097-0215(200002)9999:9999<::AID-IJC1052>3.3.CO;2-N ER - TY - JOUR AU - Brouwer, R. AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van PY - 2001 UR - https://hdl.handle.net/2066/186939 AB - The anti-PM/Scl autoantibodies are known to characterize a subset of autoimmune patients with myositis, scleroderma (Scl), and the PM/Scl overlap syndrome. The major autoantigens that are recognized by anti-PM/Scl autoantibodies are designated PM/Scl-100 and PM/Scl-75. These autoantigens have been reported to associate into a large complex consisting of 11 to 16 proteins and to play a role in ribosome synthesis. Recently, it was discovered that the PM/Scl complex is the human counterpart of the yeast (Saccharomyces cerevisiae) exosome, which is an RNA-processing complex consisting of 11 3' --> 5' exoribonucleases. To date, 10 human exosome components have been identified, although only some of these were studied in more detail. In this review, we discuss some recent advances in the characterization of the PM/Scl complex. TI - The human exosome: an autoantigenic complex of exoribonucleases in myositis and scleroderma. EP - 106 SN - 1478-6354 IS - iss. 2 SP - 102 JF - Arthritis Research & Therapy VL - vol. 3 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/186939/186939.pdf?sequence=1 ER - TY - JOUR AU - Brouwer, R. AU - Hengstman, G.J.D. AU - Vree Egberts, W.T.M. AU - Ehrfeld, H. AU - Bozic, B. AU - Ghirardello, A. AU - Grondal, G. AU - Hietarinta, M. AU - Isenberg, D.A. AU - Kalden, J.R. AU - Lundberg, I. AU - Moutsopoulos, H.M. AU - Roux-Lombard, P. AU - Vencovsky, J. AU - Wikman, A. AU - Seelig, H.P. AU - Engelen, B.G.M. van AU - Venrooij, W.J.W. van PY - 2001 UR - https://hdl.handle.net/2066/185568 AB - OBJECTIVE: To determine the prevalence of myositis specific autoantibodies (MSAs) and several myositis associated autoantibodies (MAAs) in a large group of patients with myositis. METHODS: A total of 417 patients with myositis from 11 European countries (198 patients with polymyositis (PM), 181 with dermatomyositis (DM), and 38 with inclusion body myositis (IBM)) were serologically analysed by immunoblot, enzyme linked immunosorbent assay (ELISA) and/or immunoprecipitation. RESULTS: Autoantibodies were found in 232 sera (56%), including 157 samples (38%) which contained MSAs. The most commonly detected MSA was anti-Jo-1 (18%). Other anti-synthetase, anti-Mi-2, and anti-SRP autoantibodies were found in 3%, 14%, and 5% of the sera, respectively. A relatively high number of anti-Mi-2 positive PM sera were found (9% of PM sera). The most commonly detected MAA was anti-Ro52 (25%). Anti-PM/Scl-100, anti-PM/Scl-75, anti-Mas, anti-Ro60, anti-La, and anti-U1 snRNP autoantibodies were present in 6%, 3%, 2%, 4%, 5%, and 6% of the sera, respectively. Remarkable associations were noticed between anti-Ro52 and anti-Jo-1 autoantibodies and, in a few sera, also between anti-Jo-1 and anti-SRP or anti-Mi-2 autoantibodies. CONCLUSIONS: The incidence of most of the tested autoantibody activities in this large group of European patients is in agreement with similar studies of Japanese and American patients. The relatively high number of PM sera with anti-Mi-2 reactivity may be explained by the use of multiple recombinant fragments spanning the complete antigen. Furthermore, our data show that some sera may contain more than one type of MSA and confirm the strong association of anti-Ro52 with anti-Jo-1 reactivity. TI - Autoantibody profiles in the sera of European patients with myositis. EP - 23 SN - 0003-4967 IS - iss. 2 SP - 116- JF - Annals of the Rheumatic Diseases VL - vol. 60 DO - https://doi.org/10.1136/ard.60.2.116 ER - TY - JOUR AU - Fabini, G AU - Raijmakers, R. AU - Hayer, S AU - Fouraux, M.A. AU - Pruijn, G.J.M. AU - Steiner, G. PY - 2001 UR - https://hdl.handle.net/2066/185801 AB - The hY RNAs are a group of four small cytoplasmic RNAs of unknown function that are stably associated with at least two proteins, Ro60 and La, to form Ro ribonucleoprotein complexes. Here we show that the heterogeneous nuclear ribonucleoproteins (hnRNP) I and K are able to associate with a subset of hY RNAs in vitro and demonstrate these interactions to occur also in vivo in a yeast three-hybrid system. Experiments performed in vitro and in vivo with deletion mutants of hY1 RNA revealed its pyrimidine-rich central loop to be involved in interactions with both hnRNP I and K and clearly showed their binding sites to be different from the Ro60 binding site. Both hY1 and hY3 RNAs coprecipitated with hnRNP I in immunoprecipitation experiments performed with HeLa S100 extracts and cell extracts from COS-1 cells transiently transfected with VSV-G-tagged hnRNP-I, respectively. Furthermore, both anti-Ro60 and anti-La antibodies coprecipitated hnRNP I, whereas coprecipitation of hnRNP K was not observed. Taken together, these data strongly suggest that hnRNP I is a stable component of a subpopulation of Ro RNPs, whereas hnRNP K may be transiently bound or interact only with (rare) Y RNAs that are devoid of Ro60 and La. Given that functions related to translation regulation have been assigned to both proteins and also to La, our findings may provide novel clues toward understanding the role of Y RNAs and their respective RNP complexes. TI - The heterogeneous nuclear ribonucleoproteins I and K interact with a subset of the ro ribonucleoprotein-associated Y RNAs in vitro and in vivo. EP - 20718 SN - 1083-351X IS - iss. 23 SP - 20711 JF - Journal of Biological Chemistry VL - vol. 276 DO - https://doi.org/10.1074/jbc.M101360200 ER - TY - JOUR AU - Brouwer, R. AU - Allmang, C. AU - Raijmakers, R. AU - Aarssen, Y.A.W.G. van AU - Egberts, W.V. AU - Petfalski, E. AU - Venrooij, W.J.W. van AU - Tollervey, D. AU - Pruijn, G.J.M. PY - 2001 UR - https://hdl.handle.net/2066/186951 AB - The yeast exosome is a complex of 3' --> 5' exoribonucleases. Sequence analysis identified putative human homologues for exosome components, although several were found only as expressed sequence tags. Here we report the cloning of full-length cDNAs, which encode putative human homologues of the Rrp40p, Rrp41p, and Rrp46p components of the exosome. Recombinant proteins were expressed and used to raise rabbit antisera. In Western blotting experiments, these decorated HeLa cell proteins of the predicted sizes. All three human proteins were enriched in the HeLa cells nucleus and nucleolus, but were also clearly detected in the cytoplasm. Size exclusion chromatography revealed that hRrp40p, hRrp41p, and hRrp46p were present in a large complex. This cofractionated with the human homologues of other exosome components, hRrp4p and PM/Scl-100. Anti-PM/Scl-positive patient sera coimmunoprecipitated hRrp40p, hRrp41p, and hRrp46p demonstrating their physical association. The immunoprecipitated complex exhibited 3' --> 5' exoribonuclease activity in vitro. hRrp41p was expressed in yeast and shown to suppress the lethality of genetic depletion of yeast Rrp41p. We conclude that hRrp40p, hRrp41p, and hRrp46p represent novel components of the human exosome complex. TI - Three novel components of the human exosome. EP - 6184 SN - 1083-351X IS - iss. 9 SP - 6177 JF - Journal of Biological Chemistry VL - vol. 276 DO - https://doi.org/10.1074/jbc.M007603200 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/186951/186951.pdf?sequence=1 ER - TY - JOUR AU - Kempen, L.C.L.T. van AU - Nelissen, J.M. AU - Degen, W.G.J. AU - Torensma, R. AU - Weidle, U.H. AU - Bloemers, H.P.J. AU - Figdor, C.G. AU - Swart, G.W.M. PY - 2001 UR - https://hdl.handle.net/2066/143807 AB - Activated leukocyte cell adhesion molecule (ALCAM/CD166), a member of the immunoglobulin superfamily with five extracellular immunoglobulin-like domains, facilitates heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. While expressed in a wide variety of tissues and cells, ALCAM is restricted to subsets of cells usually involved in dynamic growth and/or migration processes. A structure-function analysis, using two monoclonal anti-ALCAM antibodies and a series of amino-terminally deleted ALCAM constructs, revealed that homophilic cell adhesion depended on ligand binding mediated by the membrane-distal amino-terminal immunoglobulin domain and on avidity controlled by ALCAM clustering at the cell surface involving membrane-proximal immunoglobulin domains. Co-expression of a transmembrane ALCAM deletion mutant, which lacks the ligand binding domain, and endogenous wild-type ALCAM inhibited homophilic cell-cell interactions by interference with ALCAM avidity, while homophilic, soluble ligand binding remained unaltered. The extracellular structures of ALCAM thus provide two structurally and functionally distinguishable modules, one involved in ligand binding and the other in avidity. Functionality of both modules is required for stable homophilic ALCAM-ALCAM cell-cell adhesion. TI - Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction. EP - 25790 SN - 1083-351X IS - iss. 28 SP - 25783 JF - Journal of Biological Chemistry VL - vol. 276 DO - https://doi.org/10.1074/jbc.M011272200 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/143807/143807.pdf?sequence=1 ER - TY - JOUR AU - Eenennaam, H. van AU - Lugtenberg, T.M. AU - Vogelzangs, J.H.P. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2001 UR - https://hdl.handle.net/2066/216408 AB - The RNase MRP and RNase P particles both function as endoribonucleases. RNase MRP has been implicated in the processing of precursor-rRNA, whereas RNase P has been shown to function in the processing of pre-tRNA. Both ribonucleoprotein particles have an RNA component that can be folded into a similar secondary structure and share several protein components. We have identified human, rat, mouse, cow, and Drosophila homologues of the Pop5p protein subunit of the yeast RNase MRP and RNase P complexes. The human Pop5 cDNA encodes a protein of 163 amino acids with a predicted molecular mass of 18.8 kDa. Polyclonal antibodies raised against recombinant hPop5 identified a 19-kDa polypeptide in HeLa cells and showed that hPop5 is associated with both RNase MRP and RNase P. Using affinity-purified anti-hPop5 antibodies, we demonstrated that the endogenous hPop5 protein is localized in the nucleus and accumulates in the nucleolus, which is consistent with its association with RNase MRP and RNase P. Catalytically active RNase P was partially purified from HeLa cells, and hPop5 was shown to be associated with it. Finally, the evolutionarily conserved acidic C-terminal tail of hPop5 appeared to be required neither for complex formation nor for RNase P activity. TI - hPop5, a protein subunit of the human RNase MRP and RNase P endoribonucleases. EP - 31641 SN - 1083-351X IS - iss. 34 SP - 31635 JF - Journal of Biological Chemistry VL - vol. 276 DO - https://doi.org/10.1074/jbc.M103399200 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Engelen, B.G.M. van AU - Vree Egberts, W.T.M. AU - Venrooij, W.J.W. van PY - 2001 UR - https://hdl.handle.net/2066/191674 AB - Myositis-specific autoantibodies (MSAs) are found in almost half the patients with an idiopathic inflammatory myopathy (IIM). Several clinical and epidemiological studies have suggested that MSAs are associated with specific clinical characteristics. Some of these associations are well-defined and are of clinical significance ( eg, anti-Jo-1 and the anti-synthetase syndrome), others are less well established and can cause unnecessary anxiety for both patients and physicians ( eg, anti-SRP and cardiac involvement). In this review, an overview is given of the various MSAs, their biochemical background, their clinical usefulness, and the promises they hold for a better understanding of IIM. TI - Myositis-specific autoantibodies: overview and recent developments. EP - 82 SN - 1040-8711 IS - iss. 6 SP - 476- JF - Current Opinion in Rheumatology VL - vol. 13 DO - https://doi.org/10.1097/00002281-200111000-00004 ER - TY - JOUR AU - Roeffen, W.F.G. AU - Raats, J.M.H. AU - Teelen, K.A.E.M. AU - Hoet, R.M.A. AU - Eling, W.M.C. AU - Venrooij, W.J.W. van AU - Sauerwein, R.W. PY - 2001 UR - https://hdl.handle.net/2066/216407 AB - We report the first construction of two combinatorial human phage display libraries derived from malaria-immune patients. Specific single-chain Fv fragments (scFv) against Pfs48/45, a gamete surface protein of the sexual stages of Plasmodium falciparum, were selected and analyzed extensively. The selected scFv reacted with the surface of extracellular sexual forms of the parasite and showed Pfs48/45 reactivity on immunoblot. The scFv inhibit binding of human malaria sera to native Pfs48/45 from gametocytes. Moreover, the scFv bind to target epitopes of Pfs48/45 exposed in natural infections. Sequence analysis of eight scFv clones specific for epitope III of Pfs48/45 revealed that these clones could be divided into one V(H) family-derived germ-line gene (V(H)1) and two V(L) family segments (V(L)2 and V(K)I). TI - Recombinant human antibodies specific for the Pfs48/45 protein of the malaria parasite Plasmodium falciparum. EP - 19811 SN - 1083-351X IS - iss. 23 SP - 19807 JF - Journal of Biological Chemistry VL - vol. 276 DO - https://doi.org/10.1074/jbc.M100562200 ER - TY - JOUR AU - Eenennaam, H. van AU - Jarrous, N. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2000 UR - https://hdl.handle.net/2066/185867 AB - In the past decade, important advances have been made in our knowledge of the composition of human RNase MRP and RNase P complexes. Both ribonucleoprotein particles function as endonucleases and contain RNA components that are structurally related. RNase MRP has been suggested to be involved in the processing of precursor rRNA; RNase P, in the maturation of tRNA. Here we give an overview of current data on the structure and function of human RNase MRP and RNase P particles, with emphasis on their molecular composition. At present, seven protein subunits, probably all associated with both ribonucleoprotein particles, have been isolated and their corresponding cDNAs cloned. Although no known structural motifs can be identified in the amino acid sequences of these proteins, the majority is clearly rich in basic residues. For two protein subunits, a cluster of basic amino acids have been shown to be involved in nucleolar accumulation, whereas another protein, which lacks such a region, probably enters the nucleolus by way of a piggyback mechanism. The binding regions for several of the protein subunits on the RNA have been identified, and the data have been used to create a putative structural model for the RNase MRP particle. The rather obscure situation concerning the association of the autoantigenic Th-40 protein and its possible relationship with one of the subunits, Rpp38, is discussed. TI - Architecture and function of the human endonucleases RNase P and RNase MRP. EP - 272 SN - 1521-6543 IS - iss. 4 SP - 265 JF - International Union of Biochemistry and Molecular Biology Life VL - vol. 49 ER - TY - JOUR AU - Hofmann, U.B. AU - Westphal, J.R. AU - Zendman, A.J.W. AU - Becker, J.C. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 2000 UR - https://hdl.handle.net/2066/187058 TI - Expression and activation of matrix metalloproteinase-2 (MMP-2) and its co-localization with membrane-type1I matrix metalloproteinase (MT1-MMP) correlate with melanoma progression EP - 256 SN - 0022-3417 SP - 245 JF - Journal of Pathology VL - vol. 191 ER - TY - JOUR AU - Clemens, M.J. AU - Venrooij, W.J.W. van AU - Putte, L.B.A. van de PY - 2000 UR - https://hdl.handle.net/2066/186996 TI - Apoptosis and Autoimmunity EP - 133 SN - 1350-9047 SP - 131 JF - Cell Death and Differentiation VL - vol. 7 PS - 3 p. ER - TY - JOUR AU - Kok, J.B. de AU - Balken, M.R. van AU - Roelofs, R.W.H.M. AU - Aarssen, Y.A.W.G. van AU - Swinkels, D.W. AU - Klein Gunnewiek, J.M.T. PY - 2000 UR - https://hdl.handle.net/2066/187160 TI - Quantification of hTERT mRNA and telomerase activity in bladder washings of patients with recurrent urothelial carcinomas EP - 2007 SN - 0009-9147 IS - iss. 12 SP - 2003 JF - Clinical Chemistry VL - vol. 46 ER - TY - JOUR AU - Rodenburg, R.J.T. AU - Ganga, A. AU - Lent, P.L.E.M. van AU - Putte, L.B.A. van de AU - Venrooij, W.J.W. van PY - 2000 UR - https://hdl.handle.net/2066/187200 TI - The antiinflammatory drug sulfasalazine inhibits tumor necrosis factor a expression in macrophages by inducing apoptis EP - 1950 SN - 0004-3591 IS - iss. 9 SP - 1941 JF - Arthritis and Rheumatism VL - vol. 43 PS - 10 p. DO - http://dx.doi.org/10.1002/1529-0131(200009)43:9<1941::AID-ANR4>3.0.CO;2-O ER - TY - JOUR AU - Lawley, W. AU - Doherty, A AU - Denniss, S AU - Chauhan, D AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van AU - Lunec, J AU - Herbert, K PY - 2000 UR - https://hdl.handle.net/2066/184613 AB - OBJECTIVE: In vitro treatment with ultraviolet B (UVB) induces relocalization of lupus autoantigens to the cell surface. We have addressed the relationship between autoantigen relocalization, accumulation of intracellular reactive oxygen species (ROS) and the induction of apoptosis following UVA and UVB exposure. METHODS: Human primary keratinocytes were exposed in vitro to doses of UVA and UVB equivalent to 0.01-4 times the minimal erythemal dose. The cellular locations of Ro60, Ro52, Sm, U2-B" and La were determined using monoclonal antibodies. ROS accumulation and apoptosis induction were assessed using the intracellular ROS probe 2'7'-dichlorodihydrofluorescein diacetate, and the viability stains Hoechst 33342 and propidium iodide. RESULTS: UV treatment induced the relocalization of all five autoantigens investigated and an accumulation of ROS. UVA and UVB induced necrosis and apoptosis, respectively. CONCLUSION: These data suggest that both UVA and UVB induce ROS within keratinocytes but have significantly different effects upon autoantigen relocalization and cell viability. TI - Rapid lupus autoantigen relocalization and reactive oxygen species accumulation following ultraviolet irradiation of human keratinocytes. EP - 261 SN - 1462-0324 IS - iss. 3 SP - 253 JF - Rheumatology VL - vol. 39 DO - http://dx.doi.org/10.1093/rheumatology/39.3.253 ER - TY - JOUR AU - Broekhuis, C.H. AU - Neubauer, G. AU - Heijden, A.G. van der AU - Mann, M. AU - Proud, C.G. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2000 UR - https://hdl.handle.net/2066/187036 AB - The La (SS-B) autoantigen is an evolutionarily conserved phosphoprotein which plays an important role, most likely as an RNA chaperone, in various processes, such as the biosynthesis and maturation of RNA polymerase III transcripts in the cell nucleus and (internal) initiation of translation in the cytoplasm. In this study, the phosphorylation state of this protein from human HeLa and HEp-2 cells was characterized by high-resolution two-dimensional IEF/SDS-PAGE analysis, and phosphorylation sites were mapped by nanoelectrospray mass spectrometry. Furthermore, the effect of phosphorylation at the sites identified on the subcellular distribution of the protein was studied by site-directed mutagenesis. At least 14 isoelectric isoforms were discerned on 2-D gels with La protein from both types of cells. Metabolic labeling in combination with alkaline phosphatase treatment revealed that only a limited number of these isoforms could be attributed to phosphorylation. Four phosphorylation sites, Thr-302, Ser-325, Thr-362, and Ser-366, were mapped by mass spectrometric analysis of the isolated La protein from HeLa cells or the carboxy-terminal half of this protein. The analysis of mutants of La, in which the respective phosphorylated residues were replaced by either a neutral (alanine) or an acidic (aspartate) residue, by microinjection into Xenopus laevis oocytes on the one hand and transfection of HEp-2 cells on the other hand revealed that the subcellular distribution of this protein was not affected by these amino acid substitutions. These results strongly suggest that the signals that determine the subcellular distribution of this protein are not regulated by (de)phosphorylation of the target residues examined. TI - Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution. EP - 3033 SN - 0006-2960 IS - iss. 11 SP - 3023 JF - Biochemistry VL - vol. 39 DO - http://dx.doi.org/10.1021/bi992308c ER - TY - JOUR AU - Fabini, G AU - Rutjes, S.A. AU - Zimmermann, C. AU - Pruijn, G.J.M. AU - Steiner, G. PY - 2000 UR - https://hdl.handle.net/2066/186993 AB - Human Ro ribonucleoproteins (RNPs) are composed of one of the four small Y RNAs and at least two proteins, Ro60 and La; association of additional proteins including the Ro52 protein and calreticulin has been suggested, but clear-cut evidence is still lacking. Partial purification of Ro RNPs from HeLa S100 extracts allowed characterization of several subpopulations of Ro RNPs with estimated molecular masses of between 150 and 550 kDa. The majority of these complexes contained Ro60 and La, whereas only a small proportion of Ro52 appeared to be associated with Ro RNPs. To identify novel Y RNA-associated proteins in vitro, binding of cytoplasmic proteins to biotinylated Y RNAs was investigated. In these reconstitution experiments, several proteins with estimated molecular masses of 80, 68, 65, 62, 60 and 53 kDa, the latter two being immunologically distinct from Ro60 and Ro52, respectively, appeared to bind specifically to Y RNAs. Furthermore, autoantibodies to these proteins were found in sera from patients with systemic lupus erythematosus. The proteins bound preferentially to Y1 and Y3 RNA but, with the exception of the 53-kDa protein, only weakly to Y4 RNA and not at all to Y5 RNA. Coprecipitation of the 80, 68, 65, and 53-kDa proteins by antibodies to Ro60 and La was observed, suggesting that at least a proportion of the novel proteins may reside on the same particles as La and/or Ro60. Finally, the binding sites for these proteins on Y1 RNA were clearly distinct from the Ro60-binding site involving a portion of the large central loop 2, which was found to be indispensable for binding of the 80, 68, 65 and 53-kDa proteins, as well as the stem 3-loop 3 and stem 2-loop 1 regions. Interestingly, truncation of the La-binding site resulted in decreased binding of the novel proteins (but not of Ro60), indicating La to be required for efficient association. Taken together, these results suggest the existence of further subpopulations of Ro RNPs or Y RNPs, consistent with the heterogeneous characteristics observed for these particles in the biochemical fractionation experiments. TI - Analysis of the molecular composition of Ro ribonucleoprotein complexes. Identification of novel Y RNA-binding proteins. EP - 2789 SN - 0014-2956 IS - iss. 9 SP - 2778 JF - European Journal of Biochemistry VL - vol. 267 DO - https://doi.org/10.1046/j.1432-1327.2000.01298.x ER - TY - JOUR AU - Schellekens, G.A. AU - Visser, H.K.A. AU - Jong, B.A.W. de AU - Hoogen, F.H.J. van den AU - Hazes, J.M.W. AU - Breedveld, F.C. AU - Venrooij, W.J.W. van PY - 2000 UR - https://hdl.handle.net/2066/138810 TI - The diagnostic properties of rheumatoid arthritis antibodies recognizing a cyclic citrullinated peptide EP - 163 SN - 0004-3591 IS - iss. 1 SP - 155 JF - Arthritis and Rheumatism VL - vol. 43 PS - 9 p. DO - https://doi.org/10.1002/1529-0131(200001)43:1<155::AID-ANR20>3.0.CO;2-3 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Venrooij, W.J.W. van AU - Vencovsky, J. AU - Moutsopoulos, H.M. AU - Engelen, B.G.M. van PY - 2000 UR - https://hdl.handle.net/2066/187503 TI - The relative prevalence of dermatomyositis and polymyositis in Europe exhibits a latitudinal gradient. EP - 142 SN - 0003-4967 SP - 141 JF - Annals of the Rheumatic Diseases VL - vol. 59 DO - https://doi.org/10.1136/ard.59.2.141 ER - TY - JOUR AU - Westphal, J.R. AU - Hullenaar, H.G.M. van het AU - Peek, R. AU - Willems, H.W. AU - Crickard, K. AU - Crickard, U. AU - Askaa, J. AU - Clemmensen, I. AU - Ruiter, D.J. AU - Waal, R.M.W. de PY - 2000 UR - https://hdl.handle.net/2066/144743 TI - Angiogenetic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo EP - 776 SN - 0020-7136 SP - 768 JF - International Journal of Cancer VL - vol. 86 DO - https://doi.org/10.1002/(SICI)1097-0215(20000615)86:6<768::AID-IJC3>3.3.CO;2-5 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Venrooij, W.J.W. van AU - Vree Egberts, W.T.M. AU - Engelen, B.G.M. van PY - 2000 UR - https://hdl.handle.net/2066/191675 TI - Clinical and serological characteristics of myositis: myositis specific autoantibodies aid in the differential diagnosis of the idiopathic inflammatory myopathies. SN - 0028-3878 IS - iss. suppl.3 SP - A240 JF - Neurology VL - vol. 54 ER - TY - JOUR AU - Teunissen, S.W. AU - Kruithof, M.J. AU - Farris, A.D. AU - Harley, J.B. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 2000 UR - https://hdl.handle.net/2066/184433 AB - In this study, phylogenetically conserved structural features of the Ro RNP associated Y RNAs were investigated. The human, iguana, and frog Y3 and Y4 RNA sequences have been determined previously and the respective RNAs were subjected to enzymatic and chemical probing to obtain structural information. For all of the analyzed RNAs, the probing data were used to compose secondary structures, which partly deviate from previously predicted structures. Our results confirm the existence of two stem structures, which are also found at similar positions in hY1 and hY5 RNA. For the remaining parts of hY3 and hY4 RNA the secondary structures differ from those previously proposed based upon computer predictions. What might be more important is that certain parts of the RNAs appear to be flexible, i.e., to adopt several conformations. Another striking feature is that a characteristic pyrimidine-rich region, present in every Y RNA known, is single-stranded in all secondary structures. This may suggest that this region is readily available for base pairing inter-actions with other cellular nucleic acids, which might be important for the as yet unknown function of the RNAs. TI - Conserved features of Y RNAs: a comparison of experimentally derived secondary structures. EP - 619 SN - 0305-1048 IS - iss. 2 SP - 610 JF - Nucleic Acids Research VL - vol. 28 DO - http://dx.doi.org/10.1093/nar/28.2.610 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/184433/184433.pdf?sequence=1 ER - TY - JOUR AU - Kempen, L.C.L.T. van AU - Oord, J.J. van den AU - Muijen, G.N.P. van AU - Weidle, U.H. AU - Bloemers, H.P.J. AU - Swart, G.W.M. PY - 2000 UR - https://hdl.handle.net/2066/187500 TI - Activated leukocyte cell adhesion molecule/CD166, a marker of tumor progression in primary malignant melanoma of the skin EP - 774 SN - 0002-9440 SP - 769 JF - American Journal of Pathology VL - vol. 156 DO - https://doi.org/10.1016/S0002-9440(10)64943-7 ER - TY - JOUR AU - Ali, N. AU - Pruijn, G.J.M. AU - Kenan, D.J. AU - Keene, J.D. AU - Siddiqui, A. PY - 2000 UR - https://hdl.handle.net/2066/184508 AB - The 5'-noncoding region (5'-NCR) of the hepatitis C virus (HCV) RNA genome serves as an internal ribosome entry site (IRES) and mediates translation initiation in a cap-independent manner. Previously, we reported the interaction between La antigen and the HCV IRES, which appeared to occur in the context of initiator AUG. It was further shown that HCV IRES-mediated translation was stimulated in the presence of human La antigen. In this study, we have defined the cis- and trans-acting elements responsible for La-5'-NCR interactions and established the dependence of the HCV IRES efficiency on cellular La antigen. During the La-IRES interaction, initiator AUG but not the neighboring codons was found to be the direct target of La binding. The C terminus effector domain-dependent modulation of La binding to the HCV IRES is demonstrated by deletion and substitution mutagenesis of the protein. An RNA systematic evolution of ligands by exponential enrichment (SELEX), generated against La protein that selectively binds La in HeLa lysates and competes for the protein binding to the 5'-NCR, was used to demonstrate the requirement of La for the HCV IRES function in the context of mono- and dicistronic mRNAs. Sequestration of La antigen by the RNA SELEX in HeLa translation lysates blocked the HCV and poliovirus IRES-mediated translation in vitro. The functional requirement of La protein for the HCV IRES activity was further established in a liver-derived cell line and in an add-back experiment in which the inhibited IRES was rescued by recombinant human La. These results strongly argue for the novel role of La protein during selection of the initiator AUG and its participation during internal initiation of translation of the HCV RNA genome. TI - Human La antigen is required for the hepatitis C virus internal ribosome entry site-mediated translation. EP - 27540 SN - 1083-351X IS - iss. 36 SP - 27531 JF - Journal of Biological Chemistry VL - vol. 275 ER - TY - JOUR AU - Kroot, E.J.A. AU - Jong, B.A.W. de AU - Leeuwen, M.A. van AU - Swinkels, H.L. AU - Hoogen, F.H.J. van den AU - Hof, M.A. van 't AU - Putte, L.B.A. van de AU - Rijswijk, M.H. van AU - Venrooij, W.J.W. van AU - Riel, P.L.C.M. van PY - 2000 UR - https://hdl.handle.net/2066/138811 TI - The prognostic value of anticyclic citrullinated peptide antibody in patients with recent-onset rheumatoid arthritis EP - 1835 SN - 0004-3591 SP - 1831 JF - Arthritis and Rheumatism VL - vol. 43 PS - 5 p. DO - https://doi.org/10.1002/1529-0131(200008)43:8<1831::AID-ANR19>3.0.CO;2-6 ER - TY - JOUR AU - Debeer, P. AU - Schoenmakers, E.F.P.M. AU - Thoelen, R. AU - Holvoet, M. AU - Kuittinen, T. AU - Fabry, G. AU - Fryns, J.P. AU - Goodman, F.R. AU - Ven, W.J.M. van de PY - 2000 UR - https://hdl.handle.net/2066/184597 TI - Physical map of a 1.5Mb region on 12p11.2 harbouring a synpolydactyly associated chromosomal breakpoint EP - 570 SN - 1018-4813 IS - iss. 8 SP - 561 JF - European Journal of Human Genetics VL - vol. 8 DO - https://doi.org/10.1038/sj.ejhg.5200497 ER - TY - JOUR AU - Rijk, E.P.C.T. de AU - Rijk, A.F. van AU - Esch, E. van AU - Jong, W.W.W. de AU - Wesseling, P. AU - Bloemendal, H. PY - 2000 UR - https://hdl.handle.net/2066/187034 TI - Demyelination and axonal dystrophy in alpha A-crystallin transgenic mice EP - 282 SN - 0959-9673 SP - 271 JF - International Journal of Experimental Pathology VL - vol. 81 ER - TY - JOUR AU - Degen, W.G.J. AU - Aarssen, Y.A.W.G. van AU - Pruijn, G.J.M. AU - Utz, P.J. AU - Venrooij, W.J.W. van PY - 2000 UR - https://hdl.handle.net/2066/187655 AB - During apoptosis, the U1-70K protein, a component of the spliceosomal U1 snRNP complex, is specifically cleaved by the enzyme caspase-3, converting it into a C-terminally truncated 40-kDa fragment. In this study, we show that the 40-kDa U1-70K fragment is still associated with the complete U1 snRNP complex, and that no obvious modifications occur with the U1 snRNP associated proteins U1A, U1C and Sm-B/B'. Furthermore, it is described for the first time that the U1 snRNA molecule, which is the backbone of the U1 snRNP complex, is modified during apoptosis by the specific removal of the first 5 - 6 nucleotides including the 2,2, 7-trimethylguanosine (TMG) cap. The observations that U1 snRNA cleavage is very specific (no such modifications were detected for the other U snRNAs tested) and that U1 snRNA cleavage is markedly inhibited in the presence of caspase inhibitors, indicate that an apoptotically activated ribonuclease is responsible for the specific modification of U1 snRNA during apoptosis. TI - The fate of U1 snRNP during anti-Fas induced apoptosis: specific cleavage of the U1 snRNA molecule. EP - 79 SN - 1350-9047 IS - iss. 1 SP - 70 JF - Cell Death and Differentiation VL - vol. 7 DO - https://doi.org/10.1038/sj.cdd.4400617 ER - TY - JOUR AU - Weterman, M.A.J. AU - Groningen, J.J.M. van AU - Jansen, A. AU - Geurts van Kessel, A.H.M. PY - 2000 UR - https://hdl.handle.net/2066/184578 TI - Nuclear localization and transactivating capacities of the papillary renal cell carcinoma-associated TFE3 and PRCC (fusion) proteins. EP - 74 SN - 0950-9232 IS - iss. 1 SP - 69 JF - Oncogene VL - vol. 19 DO - https://doi.org/10.1038/sj.onc.1203255 ER - TY - JOUR AU - Degen, W.G.J. AU - Pruijn, G.J.M. AU - Raats, J.M.H. AU - Venrooij, W.J.W. van PY - 2000 UR - https://hdl.handle.net/2066/224004 AB - Autoimmune diseases are frequently characterized by the presence of autoantibodies directed against nucleic acid-protein complexes present in the nucleus of the cell. The mechanisms by which these autoantigenic molecules escape immunological tolerance are largely unknown, although a number of recent observations suggest that modified self-proteins generated during apoptosis may play an important role in the development of autoimmunity. It has been hypothesized that the recognition of these modified self-proteins by the immune system may promote autoantibody production. While apoptosis is specifically characterized by posttranslational modification of proteins, recent findings also show that nucleic acids are modified. This review summarizes the specific cleavages of some of these key nucleic acids, i.e. chromosomal DNA, ribosomal RNA and small structural RNAs (U1 snRNA, Y RNA), in apoptotic cells. TI - Caspase-dependent cleavage of nucleic acids. EP - 627 SN - 1350-9047 IS - iss. 7 SP - 616 JF - Cell Death and Differentiation VL - vol. 7 DO - https://doi.org/10.1038/sj.cdd.4400672 ER - TY - JOUR AU - Farris, A.D. AU - Koelsch, G. AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van AU - Harley, J.B. PY - 1999 UR - https://hdl.handle.net/2066/184732 AB - Y RNAs are small 'cytoplasmic' RNAs which are components of the Ro ribonucleoprotein (RNP) complex. The core of this complex, which is found in the cell nuclei of higher eukaryotes as well as the cytoplasm, is composed of a complex between the 60 kDa Ro protein and Y RNAs. Human cells contain four distinct Y RNAs (Y1, Y3, Y4 and Y5), while other eukaryotes contain a variable number of Y RNA homologues. When detected in a particular species, the Ro RNP has been present in every cell type within that particular organism. This characteristic, along with its high conservation among vertebrates, suggests an important function for Ro RNP in cellular metabolism; however, this function has not yet been definitively elucidated. In order to identify conserved features of Y RNA sequences and structures which may be directly involved in Ro RNP function, a phylogenetic comparative analysis of Y RNAs has been performed. Sequences of Y RNA homologues from five vertebrate species have been obtained and, together with previously published Y RNA sequences, used to predict Y RNA secondary structures. A novel RNA secondary structure comparison algorithm, the suboptimal RNA analysis program, has been developed and used in conjunction with available algorithms to find phylogenetically conserved secondary structure models for YI, Y3 and Y4 RNAs. Short, conserved sequences within the Y RNAs have been identified and are invariant among vertebrates, consistent with a direct role for Y RNAs in Ro function. A subset of these are located wholly or partially in looped regions in the Y3 and Y4 RNA predicted model structures, in accord with the possibility that these Y RNAs base pair with other cellular nucleic acids or are sites of interaction between the Ro RNP and other macromolecules. TI - Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. EP - 1078 SN - 0305-1048 IS - iss. 4 SP - 1070 JF - Nucleic Acids Research VL - vol. 27 DO - http://dx.doi.org/10.1093/nar/27.4.1070 ER - TY - JOUR AU - IJland, S.A.J. AU - Jager, M.J. AU - Heijdra, B. AU - Westphal, J.R. AU - Peek, R. PY - 1999 UR - https://hdl.handle.net/2066/184764 TI - Expression of angiogenic and immunosuppressive factors by uveal melanoma cell lines. EP - 450 SN - 0960-8931 SP - 445 JF - Melanoma Research VL - vol. 9 DO - http://dx.doi.org/10.1097/00008390-199910000-00003 ER - TY - JOUR AU - Pluk, W.L.L.P. AU - Eenennaam, H. van AU - Rutjes, S.A. AU - Pruijn, G.J.M. AU - Venrooij, W.J.W. van PY - 1999 UR - https://hdl.handle.net/2066/187504 AB - The eukaryotic nucleolus contains a large number of small RNA molecules that, in the form of small nucleolar ribonucleoprotein complexes (snoRNPs), are involved in the processing and modification of pre-rRNA. One of the snoRNPs that has been shown to possess enzymatic activity is the RNase MRP. RNase MRP is an endoribonuclease involved in the formation of the 5' end of 5.8S rRNA. In this study the association of the hPop1 protein with the RNase MRP complex was investigated. The hPop1 protein seems not to be directly bound to the RNA component, but requires nt 1-86 and 116-176 of the MRP RNA to associate with the RNase MRP complex via protein-protein interactions. UV crosslinking followed by ribonuclease treatment and immunoprecipitation with anti-Th/To antibodies revealed three human proteins of about 20, 25, and 40 kDa that can associate with the RNase MRP complex. The 20- and 25-kDa proteins appear to bind to stem-loop I of the MRP RNA whereas the 40-kDa protein requires the central part of the MRP RNA (nt 86-176) for association with the RNase MRP complex. In addition, we show that the human RNase P proteins Rpp30 and Rpp38 are also associated with the RNase MRP complex. Expression of Vesicular Stomatitis Virus- (VSV) tagged versions of these proteins in HeLa cells followed by anti-VSV immunoprecipitation resulted in coprecipitation of both RNase P and RNase MRP complexes. Furthermore, UV crosslinking followed by anti-Th/To and anti-Rpp38 immunoprecipitation revealed that the 40-kDa protein we detected in UV crosslinking is probably identical to Rpp38. TI - RNA-protein interactions in the human RNase MRP ribonucleoprotein complex. EP - 524 SN - 1355-8382 IS - iss. 4 SP - 512 JF - Rna : a Publication of the Rna Society VL - vol. 5 DO - http://dx.doi.org/10.1017/S1355838299982079 ER - TY - JOUR AU - Rutjes, S.A. AU - Utz, P.J. AU - Heijden, A.G. van der AU - Broekhuis, C.H. AU - Venrooij, W.J.W. van AU - Pruijn, G.J.M. PY - 1999 UR - https://hdl.handle.net/2066/186150 AB - In the past few years, a role for apoptotic processes in the development of autoimmune diseases has been suggested. An increasing number of cellular proteins, which are modified during apoptosis, has been described, and many of these proteins have been identified as autoantigens. We have studied the effects of apoptosis on the La protein in more detail and for the first time demonstrate that this autoantigen is rapidly dephosphorylated after the induction of apoptosis. Dephosphorylation of the La protein was observed after induction of apoptosis by several initiators and in various cell types. Furthermore, we demonstrate that at least a subset of the La protein is proteolytically cleaved in vivo, generating a 45 kDa fragment. Dephosphorylation as well as cleavage of La is inhibited by ZnSO4 as well as by several tetrapeptide caspase inhibitors, indicating that these processes require the activation of caspases. Dephosphorylation of La is inhibited by low concentrations of okadaic acid, suggesting that a PP2A-like phosphatase is involved. Generation of the 45 kDa fragment is consistent with proteolytic cleavage at amino acids 371 and/or 374. The possible significance of the apoptotic changes in the La protein for autoantibody production is discussed. TI - The La (SS-B) autoantigen, a key protein in RNA biogenesis, is dephosphorylated and cleaved early during apoptosis. EP - 986 SN - 1350-9047 IS - iss. 10 SP - 976 JF - Cell Death and Differentiation VL - vol. 6 DO - https://doi.org/10.1038/sj.cdd.4400571 ER - TY - JOUR AU - Zendman, A.J.W. AU - Cornelissen, L.M.H.A. AU - Weidle, U.H. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 1999 UR - https://hdl.handle.net/2066/187656 TI - CTp11, a novel member of the family of human cancer/testis antigens. EP - 6229 SN - 0008-5472 SP - 6223 JF - Cancer Research VL - vol. 59 ER - TY - JOUR AU - Hildebrandt, T. AU - Preiherr, J. AU - Klostermann, S. AU - Kaul, S. AU - Zendman, A.J.W. AU - Muijen, G.N.P. van AU - Weidle, U.H. PY - 1999 UR - https://hdl.handle.net/2066/184794 TI - Identification of URIM, a novel gene up-regulated in metastasis. EP - 530 SN - 0250-7005 SP - 525 JF - Anticancer Research VL - vol. 19 ER - TY - JOUR AU - Rodenburg, R.J.T. AU - Hoogen, F.H.J. van den AU - Barrera Rico, P. AU - Venrooij, W.J.W. van AU - Putte, L.B.A. van de PY - 1999 UR - https://hdl.handle.net/2066/138816 TI - Superinduction of interleukin in 8 mRNA in activated monocyte derived macrophages from rheumatoid arthritis patients. EP - 652 SN - 0003-4967 SP - 648 JF - Annals of the Rheumatic Diseases VL - vol. 58 PS - 5 p. DO - https://doi.org/10.1136/ard.58.10.648 ER - TY - JOUR AU - James, M.C. AU - Jeffrey, I.W. AU - Pruijn, G.J.M. AU - Thijssen, J.P.H. AU - Clemens, M.J. PY - 1999 UR - https://hdl.handle.net/2066/184894 AB - The La antigen is a protein which can bind both single-stranded and double-stranded forms of RNA and has regulatory effects on gene expression at the levels of transcription and translation. It was previously shown to inhibit the activation of the dsRNA-dependent protein kinase PKR by sequestering and/or unwinding double-stranded RNA. Here, we demonstrate that, as predicted by these properties, the La antigen can rescue protein synthesis in the reticulocyte lysate system from inhibition by low concentrations of dsRNA. This effect is reversed by higher concentrations of dsRNA. Using a series of deletion mutants we have investigated the structural features of the La antigen that are required for these effects. The ability to bind dsRNA is influenced by regions within both the previously characterized N-terminal RNP motif and the C-terminal half of the protein. La mutants with either N-terminal or C-terminal deletions retain the ability to inhibit the protein kinase activity of PKR and to rescue protein synthesis from inhibition by dsRNA. It is notable that sequences in the C-terminal half of the La antigen, including a phosphorylation site at Ser366, which are needed for other regulatory effects of the protein on gene expression are dispensable for the effects of La on PKR. We suggest that La regulates PKR activity solely as a result of its ability to act as an RNA-binding protein that can compete with PKR for limiting amounts of dsRNA. TI - Translational control by the La antigen. Structure requirements for rescue of the double-stranded RNA-mediated inhibition of protein synthesis. EP - 162 SN - 0014-2956 IS - iss. 1 SP - 151 JF - European Journal of Biochemistry VL - vol. 266 DO - http://dx.doi.org/10.1046/j.1432-1327.1999.00839.x ER - TY - JOUR AU - Zendman, A.J.W. AU - Cornelissen, L.M.H.A. AU - Weidle, U.H. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 1999 UR - https://hdl.handle.net/2066/187657 TI - TM7XN1, a novel human EGF-TM7-like cDNA, detected with mRNA differential display using human melanoma cell lines with different metastatic potential. EP - 298 SN - 1873-3468 SP - 292 JF - FEBS Letters VL - vol. 446 DO - https://doi.org/10.1016/S0014-5793(99)00230-6 ER - TY - JOUR AU - Hoet, R.M.A. AU - Pieffers, M. AU - Stassen, H.W.S. AU - Raats, J.M.H. AU - Wildt, R.M.T. de AU - Pruijn, G.J.M. AU - Hoogen, F.H.J. van den AU - Venrooij, W.J.W. van PY - 1999 UR - https://hdl.handle.net/2066/138818 TI - The Importance of the Light Chain for the Epitope Specificity of Human Anti-U1 Small Nuclear RNA Autoantibodies Present in Systemic Lupus Erythematosus Patients EP - 3312 SN - 0019-2805 SP - 3304 JF - Immunology VL - vol. 163 PS - 9 p. ER - TY - JOUR AU - Esch, H. van AU - Groenen, P.J.T.A. AU - Fryns, J.P. AU - Ven, W.J.M. van de AU - Devriendt, K. PY - 1999 UR - https://hdl.handle.net/2066/186156 TI - The phenotypic spectrum of the 10p deletion syndrome versus the classical DiGeorge syndrome. EP - 65 SN - 1015-8146 SP - 59 JF - Genetic Counseling VL - vol. 10 ER - TY - JOUR AU - Esch, H. van AU - Groenen, P.J.T.A. AU - Daw, S. AU - Poffyn, A. AU - Holvoet, M. AU - Scambler, P.J. AU - Fryns, J.P. AU - Ven, W.J.M. van de AU - Devriendt, K. PY - 1999 UR - https://hdl.handle.net/2066/184833 TI - Partial DiGeorge syndrome in two patients with a 10p rearrangement. EP - 276 SN - 0009-9163 SP - 269 JF - Clinical Genetics VL - vol. 55 DO - https://doi.org/10.1034/j.1399-0004.1999.550410.x ER - TY - JOUR AU - Hofmann, U.B. AU - Westphal, J.R. AU - Waas, E.T. AU - Zendman, A.J.W. AU - Cornelissen, L.M.H.A. AU - Ruiter, D.J. AU - Muijen, G.N.P. van PY - 1999 UR - https://hdl.handle.net/2066/222105 TI - Matrix metalloproteinases in human melanoma cell lines and xenografts: increased expression of activated matrix metalloproteinase-2 (MMP-2) correlates with melanoma progression EP - 782 SN - 0007-0920 IS - iss. 5 SP - 774 JF - British Journal of Cancer VL - vol. 81 DO - https://doi.org/10.1038/sj.bjc.6690763 ER - TY - JOUR AU - Rodenburg, R.J.T. AU - Hoogen, F.H.J. van den AU - Putte, L.B.A. van de AU - Venrooij, W.J.W. van PY - 1998 UR - https://hdl.handle.net/2066/138831 TI - Peripheral blood monocytes of rheumatoid arthritis patients do not express elevated TNF a, IL-1B and IL-8 mRNA levels. A comparison of monocyte isolation procedures EP - 175 SN - 0022-1759 SP - 169 JF - Journal of Immunological Methods VL - vol. 221 PS - 7 p. DO - http://dx.doi.org/10.1016/S0022-1759(98)00183-5 ER - TY - JOUR AU - Rodenburg, R.J.T. AU - Brinkhuis, R.F. AU - Peek, R. AU - Westphal, J.R. AU - Hoogen, F.H.J. van den AU - Venrooij, W.J.W. van AU - Putte, L.B.A. van de PY - 1998 UR - https://hdl.handle.net/2066/138825 TI - Expression of macrophage-derived chemokine (MDC)mRNA in macrophages is enhanced by interleukin-1B, tumor necrosis factor a, and lipopolysaccharide EP - 611 SN - 0741-5400 SP - 606 JF - Journal of Leukocyte Biology VL - vol. 63 PS - 6 p. DO - http://dx.doi.org/10.1002/jlb.63.5.606 ER - TY - JOUR AU - Hengstman, G.J.D. AU - Engelen, B.G.M. van AU - Badrising, U.A. AU - Hoogen, F.H.J. van den AU - Venrooij, W.J.W. van PY - 1998 UR - https://hdl.handle.net/2066/138834 TI - Presence of the anti-Jo1 autoantibody excludes inclusion body myositis EP - 423 SN - 0364-5134 SP - 423 JF - Annals of Neurology VL - vol. 44 PS - 1 p. ER - TY - JOUR AU - Verbraak, F.D. AU - Bruinenberg, M. AU - Horn, G.J. van den AU - Meenken, C. AU - Leij, A. van der AU - Hoyng, C.B. AU - Kijlstra, A. AU - Peek, R. PY - 1998 UR - https://hdl.handle.net/2066/188580 TI - Cytomegalovirus (CMV) strain differences between the eye and blood of AIDS patients with CMV retinitis EP - 718 SN - 0269-9370 SP - 713 JF - Aids VL - vol. 12 DO - https://doi.org/10.1097/00002030-199807000-00007 ER - TY - JOUR AU - Kuppevelt, A.H.M.S.M. van AU - Dennissen, M.A.B.A. AU - Venrooij, W.J.W. van AU - Hoet, R.M.A. AU - Veerkamp, J.H. PY - 1998 UR - https://hdl.handle.net/2066/123031 AB - Detailed analysis of various heparan sulfate (HS) species is seriously hampered by a lack of appropriate tools, such as antibodies. We adopted phage display technology to generate anti-HS antibodies. A "single pot" semisynthetic human antibody phage display library was subjected to four rounds of selection on HS from bovine kidney using panning methodology. Three different phage clones expressing anti-HS single chain variable fragment antibodies (HS4C3, HS4D10, and HS3G8) were isolated, with an amino acid sequence of the complementarity-determining region 3 of GRRLKD (VH3 gene, DP-38), SLRMNGCGAHQ (VH3 gene, DP-42), and YYHYKVN (VH1 gene, DP-8), respectively. The antibodies react with HS and heparin, but not with DNA or other glycosaminoglycans. Kd values for HS are about 0.1 microM. The three antibodies react differently toward various HS preparations and show different staining patterns on rat kidney sections, indicating recognition of different HS molecules. This also holds for two described mouse anti-HS IgMs (JM403 and 10E4; both generated by conventional hybridoma technique) and indicates the presence of at least 5 different HS species in the kidney. O- and N-sulfation are important for binding of HS to HS4C3 and HS3G8. The three single chain antibodies, but not JM403, block a basic fibroblast growth factor binding site of HS. It is concluded that phage display technology presents a powerful technique to generate antibodies specific for HS epitopes. This is the first time this technique has been successfully applied to obtain directly antibodies to (poly)saccharides. TI - Generation and application of type-specific anti-heparan sulfate antibodies using phage display technology. Further evidence for heparan sulfate heterogeneity in the kidney. EP - 12966 SN - 1083-351X IS - iss. 21 SP - 12960 JF - Journal of Biological Chemistry VL - vol. 273 DO - https://doi.org/10.1074/jbc.273.21.12960 ER - TY - JOUR AU - Kuppevelt, A.H.M.S.M. van AU - Dennissen, M.A.B.A. AU - Venrooij, W.J.W. van AU - Hoet, R.M.A. AU - Veerkamp, J.H. PY - 1998 UR - https://hdl.handle.net/2066/123030 TI - Generation and application of type-specific anti-heparan sulfate antibodies using phage display technology. Further evidence for heparan sulfate heterogeneity in the kidney EP - 12966 SN - 1083-351X SP - 12960 JF - Journal of Biological Chemistry VL - vol. 273 DO - https://doi.org/10.1074/jbc.273.21.12960 ER - TY - JOUR AU - Hoet, R.M.A. AU - Raats, J.M.H. AU - Wildt, R.M.T. de AU - Dumortier, H. AU - Muller, S.H. AU - Hoogen, F.H.J. van den AU - Venrooij, W.J.W. van PY - 1998 UR - https://hdl.handle.net/2066/138826 TI - Human monoclonal autoantibody fragments from combinatorial antibody libraries directed to the U1snRNP associated U1C protein; epitope mapping, immunolocallization and V-gene usage EP - 1055 SN - 0161-5890 SP - 1045 JF - Molecular Immunology VL - vol. 35 PS - 11 p. DO - https://doi.org/10.1016/S0161-5890(98)00093-5 ER - TY - JOUR AU - Wagener, D.J.T. AU - Vermorken, A.J.M. AU - Hansen, H.H.G. AU - Hossfeld, D.K. PY - 1998 UR - https://hdl.handle.net/2066/159297 TI - The ESMO programme of certification and training for medical oncology EP - 587 SN - 0923-7534 SP - 585 JF - Annals of Oncology VL - vol. 9 DO - https://doi.org/10.1023/A:1008276911705 ER - TY - JOUR AU - Klundert, F.A.J.M. van de AU - Smulders, R.H. AU - Gijsen, M.L.J. AU - Lindner, R.A. AU - Jaenicke, R. AU - Carver-Ward, J.A. AU - Jong, W.W.W. de PY - 1998 UR - https://hdl.handle.net/2066/186208 TI - The mammalian small heat-shock protein Hsp20 forms dimers and is a poor chaperone. EP - 1021 SN - 0014-2956 SP - 1014 JF - European Journal of Biochemistry VL - vol. 258 DO - https://doi.org/10.1046/j.1432-1327.1998.2581014.x ER - TY - JOUR AU - Brouwer, R. AU - Vree Egberts, W.T.M. AU - Jongen, P.J.H. AU - Engelen, B.G.M. van AU - Venrooij, W.J.W. van PY - 1998 UR - https://hdl.handle.net/2066/192842 TI - Anti-Frequent occurrence of anti-tRNAhis autoantibodies that recognize a conformational epitope in sera of patients with myositis EP - 1437 SN - 0004-3591 SP - 1428 JF - Arthritis and Rheumatism VL - vol. 41 DO - https://doi.org/10.1002/1529-0131(199808)41:8<1428::AID-ART12>3.0.CO;2-J ER - TY - JOUR AU - Willems, P.M.W. AU - Hoet, R.M.A. AU - Huys, E. AU - Raats, J.M.H. AU - Mensink, E.J.B.M. AU - Raymakers, R.A.P. PY - 1998 UR - https://hdl.handle.net/2066/222501 TI - Specific detection of myeloma plasma cells using anti-idiotypic single chain antibody fragments selected from a phage display library EP - 1302 SN - 0887-6924 SP - 1295 JF - Leukemia VL - vol. 12 DO - https://doi.org/10.1038/sj.leu.2401123 ER - TY - JOUR AU - Degen, W.G.J. AU - Kempen, L.C.L.T. van AU - Gijzen, E.G. AU - Groningen, G.J. AU - Kooyk, Y. van PY - 1998 UR - https://hdl.handle.net/2066/196734 TI - MEMD, a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell adhesion molecule) EP - 813 SN - 0002-9440 IS - iss. 3 SP - 805 JF - American Journal of Pathology VL - vol. 152 ER - TY - JOUR AU - Huhn, P. AU - Pruijn, G.J.M. AU - Venrooij, W.J. van AU - Bachmann, M. PY - 1997 UR - https://hdl.handle.net/2066/29563 PB - Oxford univ press TI - Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme EP - 416 SN - 0305-1048 IS - iss. 2 SP - 410 JF - Nucleic Acids Research VL - vol. 25 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/29563/29563.pdf?sequence=1 ER - TY - JOUR AU - Muchowski, P.J. AU - Bassuk, J.A. AU - Lubsen, N.H. AU - Clark, J.I. PY - 1997 UR - https://hdl.handle.net/2066/27840 PB - Amer soc biochemistry molecular biology inc TI - Human alpha B-crystallin - Small heat shock protein and molecular chaperone EP - 2582 SN - 0021-9258 IS - iss. 4 SP - 2578 JF - Journal of Biological Chemistry VL - vol. 272 ER - TY - GEN AU - Leenders, W.P.J. AU - Hinsbergh, V.W.H. van AU - Genesen, S.T. van AU - Schoenmakers, J.G.G. AU - Zoelen, E.J.J. van AU - Lubsen, N.H. PY - 1997 UR - https://hdl.handle.net/2066/24460 TI - Mutants of basic fibroblast growth factor identify different cellular response programs ER - TY - JOUR AU - Lubsen, N.H. AU - Klok, E.J. PY - 1997 UR - https://hdl.handle.net/2066/27722 PB - Lippincott-raven publ TI - Regulatory elements involved in the developmental control of rat gamma-crystallin promoters EP - 4286 SN - 0146-0404 IS - iss. 4 SP - 4286 JF - Investigative Ophthalmology and Visual Science VL - vol. 38 ER - TY - JOUR AU - Wildt, R.M.T. de AU - Ruytenbeek, R. AU - Venrooij, W.J. van AU - Hoet, R.M.A. PY - 1997 UR - https://hdl.handle.net/2066/29233 PB - Oxford univ press TI - Heavy chain CDR3 optimization of a germline encoded recombinant antibody fragment predisposed to bind the U1A protein EP - 841 SN - 0269-2139 IS - iss. 7 SP - 835 JF - Protein Engineering VL - vol. 10 ER - TY - JOUR AU - Leenders, W.P.J. AU - Genesen, S.T. van AU - Schoenmakers, J.G.G. AU - Zoelen, E.J.J. van AU - Lubsen, N.H. PY - 1997 UR - https://hdl.handle.net/2066/27662 PB - Elsevier science bv TI - Synergism between temporally distinct growth factors: bFGF, insulin and lens cell differentiation EP - 201 SN - 0925-4773 IS - iss. 2 SP - 193 JF - Mechanisms of Development VL - vol. 67 ER - TY - JOUR AU - Madsen, O. AU - Deen, P.M.T. AU - Pesole, G. AU - Saccone, C. AU - Jong, W.W. de PY - 1997 UR - https://hdl.handle.net/2066/27740 PB - Soc molecular biology evolution TI - Molecular evolution of mammalian aquaporin-2: Further evidence that elephant shrew and aardvark join the paenungulate clade EP - 371 SN - 0737-4038 IS - iss. 4 SP - 363 JF - Molecular Biology and Evolution VL - vol. 14 L1 - https://repository.ubn.ru.nl/bitstream/handle/2066/27740/27740___.PDF?sequence=1 ER - TY - JOUR AU - Brahms, H. AU - Raker, V.A. AU - Venrooij, W.J. van AU - Luhrmann, R. PY - 1997 UR - https://hdl.handle.net/2066/28974 PB - Lippincott-raven publ TI - A major, novel systemic lupus erythematosus autoantibody class recognizes the E, F, and G Sm snRNP proteins as an E-F-G complex but not in their denatured states EP - 682 SN - 0004-3591 IS - iss. 4 SP - 672 JF - Arthritis and Rheumatism VL - vol. 40 ER - TY - JOUR AU - Brunekreef, G.A. AU - Genesen, S.T. van AU - Lubsen, N.H. PY - 1997 UR - https://hdl.handle.net/2066/29001 PB - Academic press ltd TI - Sp1- and octamer-consensus sequence binding proteins during lens fibre differentiation EP - 299 SN - 0014-4835 IS - iss. 3 SP - 295 JF - Experimental Eye Research VL - vol. 64 ER - TY - JOUR AU - Boekel, M.A.M. van AU - Roll, B. 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