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| Title: | Coculture of osteoblasts and endothelial cells: optimization of culture medium and cell ratio |
| Author(s): | Ma, J. (321505948)
Beucken, J.J. van den (301085811)
Yang, F. (298982978)
Both, S.K. (297550993)
Cui, F.Z.
Pan, J.
Jansen, J.A. (070538204) |
| Publication year: | 2011 |
| Document type: | Article / Letter to editor |
| Journal: | Tissue Engineering Part C Methods |
| ISSN: | 1937-3384 |
| Volume: | vol. 17 |
| Issue: | iss. 3 |
| Start page: | p. 349 |
| End page: | p. 357 |
| Annotation: | Ma, Jinling van den Beucken, Jeroen J J P Yang, Fang Both, Sanne K Cui, Fu-Zhai Pan, Juli Jansen, John A Research Support, Non-U.S. Gov't United States Tissue Eng Part C Methods. 2011 Mar;17(3):349-57. Epub 2010 Dec 7. |
| Abstract: | Vascularization strategies in cell-based bone tissue engineering depend on optimal culture conditions. The present study aimed to determine optimal cell culture medium and cell ratio for cocultures of human marrow stromal cells (HMSCs) and human umbilical vein endothelial cells (HUVECs) in view of both osteogenic and angiogenic outcome parameters upon two-dimensional and three-dimensional culture conditions. Cultures were performed in four different media: osteoblastic cell proliferation medium, osteogenic medium (OM), endothelial medium, and a 1:1 mixture of the latter two media. Mineralization within the cocultures was observed only in OM. Subsequent experiments in OM showed that alkaline phosphatase activity, mineralization, and CD31(+) staining were highest for cocultures at a 50:50 HMSC/HUVEC ratio. Therefore, the results from the present study show that a HMSC/HUVEC coculture ratio of 50:50 in OM is the best combination to obtain both osteogenic and angiogenic differentiation. |
| Subject: | NCMLS 1C: Tissue engineering and pathology |
| Organization: | Dentistry
UMCN Extern |
| Appears in Collections: | Academic bibliography
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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2066/97727
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