DSpace at RU >
University Library >
Academic bibliography >
Files in This Item:
| ||publisher's version||445.12 kB||Adobe PDF||Under Embargo
|Title: ||Complete brain-type creatine kinase deficiency in mice blocks seizure activity and affects intracellular calcium kinetics|
|Author(s): ||Streijger, F.|
Scheenen, W.J.J.M. (138281556)
Luijtelaar, E.L.J.M. van (071899855)
Oerlemans, F.T.J.J. (298974940)
Wieringa, B. (29897357X)
Zee, C.E.E.M. van der (102968268)
|Publication year: ||2010|
|Document type: ||Article / Letter to editor|
|Volume: ||vol. 51|
|Issue: ||iss. 1|
|Start page: ||p. 79|
|End page: ||p. 88|
|Related link(s): ||http://dx.doi.org/10.1111/j.1528%2D1167.2009.02182.x|
|Abstract: ||Purpose: Brain-type creatine kinase (CK-B) and ubiquitous mitochondrial creatine kinase (UbCKmit) act as components of local phosphocreatine ATP shuttles that help in the compartmentalization and maintenance of pools of high-energy phosphate molecules in both neurons and glial cells. We investigated the role of these brain-type creatine kinases during extreme energy-demanding conditions in vivo (generalized tonic–clonic seizures) and in vitro.
Methods: The physiologic response of wild-types and mice lacking both CK-B and UbCKmit (CK--/--mice) to pentylenetetrazole (PTZ)–induced seizures was measured using electroencephalography (EEG) recordings and behavioral monitoring. In vitro intracellular Ca2+ kinetics in hippocampal granule neurons were monitored upon single and repetitive depolarizations.
Results: PTZ induced in only a few CK--/-- mice PTZ seizure-like behavior, but in all wild-types a full-blown seizure. EEG analysis showed that preseizure jerking was associated with high-amplitude discharges. Wild-type EEG recordings showed continuous runs of rhythmic 4–6 Hz activity, whereas no rhythmic EEG activities were observed in the few CK--/-- mice that developed a behavioral seizure. All other CK--/-- mice displayed a sudden postictal depression without any development of a generalized seizure. Hippocampal granule neurons of CK--/-- mice displayed a higher Ca2+ removal speed following repetitive KCl-induced depolarizations.
Discussion: Deficiency for creatine kinase is affecting brain energy metabolism and will likely contribute to the disturbance of seizure development. Because CK--/-- hippocampal neurons exhibited an increase in Ca2+ removal rate of elevated intracellular levels, we conclude that altered Ca2+ clearance in CK--/-- neurons could play a role in the abnormal EEG and seizure activity.|
|Subject: ||Biological psychology|
|Subject: ||Biologische psychologie|
|Organization: ||FSW_Fac. algemeen|
SW OZ DCC BI
|Appears in Collections:||Academic bibliography|
Please use this identifier to cite or link to this item:
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.