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| Title: | Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. |
| Author(s): | Hurst, S.F.
Kidd, S.E.
Morrissey, C.O.
Snelders, E. (314361510)
Melchers, W.J.G. (074709771)
Castelli, M.V.
Mellado, E.
Simmon, K.
Petti, C.A.
Richardson, S.
Zhang, S.
Romanelli, A.M.
Wickes, B.L.
Valk, H.A. de
Klaassen, C.H.
Balajee, S.A. |
| Publication year: | 2009 |
| Document type: | Article / Letter to editor |
| Journal: | Journal of Clinical Microbiology |
| ISSN: | 0095-1137 |
| Volume: | vol. 47 |
| Issue: | iss. 5 |
| Start page: | p. 1562 |
| End page: | p. 1564 |
| Abstract: | Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method. |
| Subject: | N4i 2: Invasive mycoses and compromised host |
| Organization: | Medical Microbiology
UMCN Extern |
| Appears in Collections: | Academic bibliography
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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2066/80126
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