Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.
Publication year
2009Source
Journal of Clinical Microbiology, 47, 5, (2009), pp. 1562-4ISSN
Publication type
Article / Letter to editor
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Organization
Medical Microbiology
Journal title
Journal of Clinical Microbiology
Volume
vol. 47
Issue
iss. 5
Page start
p. 1562
Page end
p. 4
Subject
N4i 2: Invasive mycoses and compromised host; NCMLS 1: Infection and autoimmunityAbstract
Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.
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- Academic publications [238431]
- Electronic publications [122508]
- Faculty of Medical Sciences [90364]
- Open Access publications [97504]
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