Publication year
2004Source
FEMS Microbiology Letters, 238, 2, (2004), pp. 307-313ISSN
Publication type
Article / Letter to editor
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Organization
Radboud Institute for Biological and Environmental Sciences
Journal title
FEMS Microbiology Letters
Volume
vol. 238
Issue
iss. 2
Page start
p. 307
Page end
p. 313
Subject
Ecological MicrobiologyAbstract
The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium. Entodinium furca, Ophryoseolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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