Comparison of two highly discriminatory molecular fingerprinting assays for analysis of multiple Aspergillus fumigatus isolates from patients with invasive aspergillosis.
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Publication year
2007Source
Journal of Clinical Microbiology, 45, 5, (2007), pp. 1415-9ISSN
Publication type
Article / Letter to editor
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Organization
Medical Microbiology
Blood Transfusion and Transplantation Immunology
Haematology
Biochemistry (UMC)
Journal title
Journal of Clinical Microbiology
Volume
vol. 45
Issue
iss. 5
Page start
p. 1415
Page end
p. 9
Subject
N4i 2: Invasive mycoses and compromised host; UMCN 1.5: Interventional oncologyAbstract
Two highly discriminatory fingerprinting assays, short tandem repeat typing and amplified fragment length polymorphism (AFLP), were compared to determine the genetic relatedness between 55 isolates of Aspergillus fumigatus obtained from 15 different patients suffering from proven invasive aspergillosis. Both techniques showed that interpatient isolates belonged to different genotypes and that intrapatient isolates from deep sites were all of the same genotype. By contrast, multiple genotypes were found among isolates originating from respiratory samples. Both techniques have specific advantages and disadvantages. AFLP is more universally applicable, but short tandem repeat analysis offers better discriminatory power and should be the preferred method for standardizing typing of clinical isolates of Aspergillus fumigatus.
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- Faculty of Medical Sciences [90359]
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