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| Title: | Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples |
| Author(s): | Bencini, M.A.
Brule, A.J. van den
Claas, E.C.
Hermans, M.H.
Melchers, W.J.G. (074709771)
Noordhoek, G.T.
Salimans, M.M.
Schirm, J.
Vink, C.
Zee, A. van der
Jansen, R. (298810727) |
| Publication year: | 2007 |
| Document type: | Article / Letter to editor |
| Journal: | Journal of Clinical Microbiology |
| ISSN: | 0095-1137 |
| Volume: | vol. 45 |
| Issue: | iss. 10 |
| Start page: | p. 3390 |
| End page: | p. 3392 |
| Abstract: | Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis. |
| Subject: | NCMLS 1: Immunity, infection and tissue repair
UMCN 4.1: Microbial pathogenesis and host defense |
| Organization: | UMCN Extern
Medical Microbiology
Condensed Matter Science (HFML) |
| Appears in Collections: | Academic bibliography
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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2066/36254
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