A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins
Publication year
2017Source
Methods in Molecular Biology, 1575, (2017), pp. 223-236ISSN
Publication type
Article / Letter to editor
Display more detailsDisplay less details
Organization
Biochemistry (UMC)
Journal title
Methods in Molecular Biology
Volume
vol. 1575
Page start
p. 223
Page end
p. 236
Subject
Radboudumc 19: Nanomedicine RIMLS: Radboud Institute for Molecular Life SciencesAbstract
The efficient delivery of external proteins from the external milieu to the cytosol of mammalian cells has great potential for both scientific investigations and future therapies. However, when assessing the cellular uptake of proteins, it is often difficult to distinguish between proteins that are stuck in the endosomes and those that have escaped into the cytosol. Here, we describe a method employing the prokaryotic enzyme biotin ligase that overcomes this problem and which can be employed for a highly sensitive quantification of cytosolic protein delivery.
This item appears in the following Collection(s)
- Academic publications [238441]
- Faculty of Medical Sciences [90373]
Upload full text
Use your RU credentials (u/z-number and password) to log in with SURFconext to upload a file for processing by the repository team.