Publication year
2003Source
The Faseb Journal, 17, 8, (2003), pp. 878-80ISSN
Publication type
Article / Letter to editor
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Organization
Biochemistry (UMC)
Physiology
Journal title
The Faseb Journal
Volume
vol. 17
Issue
iss. 8
Page start
p. 878
Page end
p. 80
Subject
UMCN 3.1: Neuromuscular development and genetic disorders; UMCN 5.3: Cellular energy metabolismAbstract
Little is known about the physiological functions of heparan sulfates (HSs), which are present in the basal lamina surrounding skeletal muscle fibers. Here, we present a new system in which HS is phenotypically knocked out by endogenous expression of epitope-specific anti-HS antibodies. Single-chain antibodies, containing an immunoglobulin leader peptide, were produced by using various expression systems. Antibodies were detected in the Golgi apparatus, the site of HS biosynthesis. Likewise, the HS-degrading enzyme heparanase was expressed. Endogenous expression of antibodies or heparanase in myoblasts resulted in HS-defective myotubes. Excitability and calcium kinetics of HS-defective myotubes were severely compromised, as determined by analysis of electrically induced calcium spikes via video-speed UV confocal laser scanning microscopy. Phenotypically knocking out of individual HS epitopes resulted in specific effects on excitability and calcium kinetics. These data indicate important roles for HSs in skeletal muscle calcium kinetics.
This item appears in the following Collection(s)
- Academic publications [238441]
- Faculty of Medical Sciences [90373]
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