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Publication year
2013Source
Toxicology Letters, 221, 3, (2013), pp. 219-24ISSN
Publication type
Article / Letter to editor
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Organization
Pharmacology-Toxicology
Laboratory of Genetic, Endocrine and Metabolic Diseases
Dermatology
IMM - Institute for Molecules and Materials
Journal title
Toxicology Letters
Volume
vol. 221
Issue
iss. 3
Page start
p. 219
Page end
p. 24
Subject
IGMD 7: Iron metabolism ONCOL 5: Aetiology, screening and detection; N4i 4: Auto-immunity, transplantation and immunotherapy NCEBP 1: Molecular epidemiology; NCMLS 5: Membrane transport and intracellular motility IGMD 9: Renal disorder; NCMLS 7: Chemical and physical biology IGMD 7: Iron metabolismAbstract
Hepatic fibrosis is an adverse drug reaction of methotrexate (MTX) seen after long-term use in psoriasis patients. Currently, patients are monitored for MTX-induced hepatic fibrosis by performing liver biopsy, which is risky and burdensome for the patient, or by measuring plasma procollagen type III aminopeptide (PIIINP), which is not conclusive. The objective of this study was to identify novel predictive and preferably non-invasive biomarkers to monitor psoriasis patients for MTX-induced hepatic fibrosis. Urine samples were collected from 60 psoriasis patients treated with MTX and divided into two categories: low cumulative dose (< 1500 mg MTX) and high cumulative dose (> 1500 mg). Urinary proteins were profiled using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and identified using electrospray ionization LTQ. In urine of psoriasis patients with high cumulative MTX dose multiple proteins were identified that are associated with hepatic fibrosis, such as N-cadherin, inter-alpha-trypsin inhibitor heavy chain H4, haptoglobin and serotransferrin. These proteins may be candidate urinary biomarkers to monitor MTX-induced hepatic fibrosis. In conclusion, urinary proteome analysis identified a profile of potentially predictive biomarkers for MTX-induced hepatic fibrosis in psoriasis patients with high cumulative dose of MTX.
This item appears in the following Collection(s)
- Academic publications [238430]
- Electronic publications [122512]
- Faculty of Medical Sciences [90359]
- Faculty of Science [34989]
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