Beyond KRAS mutation status: influence of KRAS copy number status and microRNAs on clinical outcome to cetuximab in metastatic colorectal cancer patients
Publication year
2012Source
BMC Cancer, 12, (2012), article 292ISSN
Publication type
Article / Letter to editor
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Organization
Pathology
IQ Healthcare
Health Evidence
Human Genetics
Medical Oncology
Former Organization
Epidemiology, Biostatistics & HTA
Journal title
BMC Cancer
Volume
vol. 12
Subject
NCEBP 2: Evaluation of complex medical interventions; NCEBP 6: Quality of nursing and allied health care; NCMLS 2: Immune Regulation ONCOL 3: Translational research; NCMLS 6: Genetics and epigenetic pathways of disease; ONCOL 3: Translational research; ONCOL 3: Translational research NCMLS 3: Tissue engineering and pathologyAbstract
ABSTRACT: BACKGROUND: KRAS mutation is a negative predictive factor for treatment with anti-epidermal growth factor receptor (EGFR) antibodies in metastatic colorectal cancer (mCRC). Novel predictive markers are required to further improve the selection of patients for this treatment. We assessed the influence of modification of KRAS by gene copy number aberration (CNA) and microRNAs (miRNAs) in correlation to clinical outcome in mCRC patients treated with cetuximab in combination with chemotherapy and bevacizumab. METHODS: Formalin-fixed paraffin-embedded primary tumour tissue was used from 34 mCRC patients in a phase III trial, who were selected based upon their good (n = 17) or poor (n = 17) progression-free survival (PFS) upon treatment with cetuximab in combination with capecitabine, oxaliplatin, and bevacizumab. Gene copy number at the KRAS locus was assessed using high resolution genome-wide array CGH and the expression levels of 17 miRNAs targeting KRAS were determined by real-time PCR. RESULTS: Copy number loss of the KRAS locus was observed in the tumour of 5 patients who were all good responders including patients with a KRAS mutation. Copy number gains in two wild-type KRAS tumours were associated with a poor PFS. In KRAS mutated tumours increased miR-200b and decreased miR-143 expression were associated with a good PFS. In wild-type KRAS patients, miRNA expression did not correlate with PFS in a multivariate model. CONCLUSIONS: Our results indicate that the assessment of KRAS CNA and miRNAs targeting KRAS might further optimize the selection of mCRC eligible for anti-EGFR therapy.
This item appears in the following Collection(s)
- Academic publications [238441]
- Electronic publications [122537]
- Faculty of Medical Sciences [90373]
- Open Access publications [97529]
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