Publication year
2012Source
Nature Protocols, 7, 10, (2012), pp. 1918-29ISSN
Publication type
Article / Letter to editor
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Organization
Dentistry
Journal title
Nature Protocols
Volume
vol. 7
Issue
iss. 10
Page start
p. 1918
Page end
p. 29
Subject
NCMLS 3: Tissue engineering and pathologyAbstract
Animal models that are reliably reproducible, appropriate analogs to the clinical condition they are used to investigate, and that offer minimal morbidity and periprocedural mortality to the subject, are the keystone to the preclinical development of translational technologies. For bone tissue engineering, a number of small animal models exist. Here we describe the protocol for one such model, the rat calvarial defect. This versatile model allows for evaluation of biomaterials and bone tissue engineering approaches within a reproducible, non-load-bearing orthotopic site. Crucial steps for ensuring appropriate experimental control and troubleshooting tips learned through extensive experience with this model are provided. The surgical procedure itself takes approximately 30 min to complete, with approximately 2 h of perioperative care, and tissue collection is generally performed 4-12 weeks postoperatively. Several analytical techniques are presented, which evaluate the cellular and extracellular matrix components, functionality and mineralization, including histological, mechanical and radiographic methods.
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- Faculty of Medical Sciences [90359]
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